{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P38777","secondaryAccessions":["D3DKZ7"],"uniProtkbId":"FSH1_YEAST","entryAudit":{"firstPublicDate":"1995-02-01","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"1995-02-01","entryVersion":168,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Family of serine hydrolases 1"},"ecNumbers":[{"value":"3.1.-.-"}]}},"genes":[{"geneName":{"value":"FSH1"},"orderedLocusNames":[{"value":"YHR049W"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14645503"}],"value":"Serine hydrolase of unknown specificity"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562095"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562095"}],"value":"Nucleus","id":"SL-0191"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562106"}],"value":"Present with 14600 molecules/cell in log phase SD medium"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the AB hydrolase 3 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":243,"modifier":"EXACT"}},"description":"Family of serine hydrolases 1","featureId":"PRO_0000212580"},{"type":"Active site","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"15802654"}]},{"type":"Active site","location":{"start":{"value":183,"modifier":"EXACT"},"end":{"value":183,"modifier":"EXACT"}},"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"15802654"}]},{"type":"Active site","location":{"start":{"value":218,"modifier":"EXACT"},"end":{"value":218,"modifier":"EXACT"}},"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"15802654"}]},{"type":"Helix","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":6,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Beta strand","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Helix","location":{"start":{"value":18,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Helix","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Beta strand","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":42,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Beta strand","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":47,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Helix","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Helix","location":{"start":{"value":59,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Beta strand","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":75,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Helix","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Helix","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Beta strand","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Helix","location":{"start":{"value":111,"modifier":"EXACT"},"end":{"value":126,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Beta strand","location":{"start":{"value":134,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Beta strand","location":{"start":{"value":145,"modifier":"EXACT"},"end":{"value":147,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Beta strand","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":157,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Helix","location":{"start":{"value":159,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Turn","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Beta strand","location":{"start":{"value":174,"modifier":"EXACT"},"end":{"value":180,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Beta strand","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":186,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Helix","location":{"start":{"value":188,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Turn","location":{"start":{"value":202,"modifier":"EXACT"},"end":{"value":204,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Turn","location":{"start":{"value":206,"modifier":"EXACT"},"end":{"value":208,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Beta strand","location":{"start":{"value":209,"modifier":"EXACT"},"end":{"value":219,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]},{"type":"Helix","location":{"start":{"value":224,"modifier":"EXACT"},"end":{"value":238,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YCD"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0719","category":"Molecular function","name":"Serine esterase"}],"references":[{"referenceNumber":1,"citation":{"id":"8091229","citationType":"journal article","authors":["Johnston M.","Andrews S.","Brinkman R.","Cooper J.","Ding H.","Dover J.","Du Z.","Favello A.","Fulton L.","Gattung S.","Geisel C.","Kirsten J.","Kucaba T.","Hillier L.W.","Jier M.","Johnston L.","Langston Y.","Latreille P.","Louis E.J.","Macri C.","Mardis E.","Menezes S.","Mouser L.","Nhan M.","Rifkin L.","Riles L.","St Peter H.","Trevaskis E.","Vaughan K.","Vignati D.","Wilcox L.","Wohldman P.","Waterston R.","Wilson R.","Vaudin M."],"citationCrossReferences":[{"database":"PubMed","id":"8091229"},{"database":"DOI","id":"10.1126/science.8091229"}],"title":"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.","publicationDate":"1994","journal":"Science","firstPage":"2077","lastPage":"2082","volume":"265"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"17322287","citationType":"journal article","authors":["Hu Y.","Rolfs A.","Bhullar B.","Murthy T.V.S.","Zhu C.","Berger M.F.","Camargo A.A.","Kelley F.","McCarron S.","Jepson D.","Richardson A.","Raphael J.","Moreira D.","Taycher E.","Zuo D.","Mohr S.","Kane M.F.","Williamson J.","Simpson A.J.G.","Bulyk M.L.","Harlow E.","Marsischky G.","Kolodner R.D.","LaBaer J."],"citationCrossReferences":[{"database":"PubMed","id":"17322287"},{"database":"DOI","id":"10.1101/gr.6037607"}],"title":"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.","publicationDate":"2007","journal":"Genome Res.","firstPage":"536","lastPage":"543","volume":"17"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"14645503","citationType":"journal article","authors":["Baxter S.M.","Rosenblum J.S.","Knutson S.","Nelson M.R.","Montimurro J.S.","Di Gennaro J.A.","Speir J.A.","Burbaum J.J.","Fetrow J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14645503"},{"database":"DOI","id":"10.1074/mcp.m300082-mcp200"}],"title":"Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast.","publicationDate":"2004","journal":"Mol. Cell. Proteomics","firstPage":"209","lastPage":"225","volume":"3"},"referencePositions":["FUNCTION"]},{"referenceNumber":5,"citation":{"id":"14562095","citationType":"journal article","authors":["Huh W.-K.","Falvo J.V.","Gerke L.C.","Carroll A.S.","Howson R.W.","Weissman J.S.","O'Shea E.K."],"citationCrossReferences":[{"database":"PubMed","id":"14562095"},{"database":"DOI","id":"10.1038/nature02026"}],"title":"Global analysis of protein localization in budding yeast.","publicationDate":"2003","journal":"Nature","firstPage":"686","lastPage":"691","volume":"425"},"referencePositions":["SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":6,"citation":{"id":"14562106","citationType":"journal article","authors":["Ghaemmaghami S.","Huh W.-K.","Bower K.","Howson R.W.","Belle A.","Dephoure N.","O'Shea E.K.","Weissman J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14562106"},{"database":"DOI","id":"10.1038/nature02046"}],"title":"Global analysis of protein expression in yeast.","publicationDate":"2003","journal":"Nature","firstPage":"737","lastPage":"741","volume":"425"},"referencePositions":["LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":7,"citation":{"id":"15802654","citationType":"journal article","authors":["Quevillon-Cheruel S.","Leulliot N.","Graille M.","Hervouet N.","Coste F.","Benedetti H.","Zelwer C.","Janin J.","Van Tilbeurgh H."],"citationCrossReferences":[{"database":"PubMed","id":"15802654"},{"database":"DOI","id":"10.1110/ps.051415905"}],"title":"Crystal structure of yeast YHR049W/FSH1, a member of the serine hydrolase family.","publicationDate":"2005","journal":"Protein Sci.","firstPage":"1350","lastPage":"1356","volume":"14"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS)"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U00062","properties":[{"key":"ProteinId","value":"AAB68901.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY558283","properties":[{"key":"ProteinId","value":"AAS56609.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006934","properties":[{"key":"ProteinId","value":"DAA06741.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S46732","properties":[{"key":"EntryName","value":"S46732"}]},{"database":"RefSeq","id":"NP_011915.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001179179.1"}]},{"database":"PDB","id":"1YCD","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.70 A"},{"key":"Chains","value":"A/B=1-243"}]},{"database":"PDBsum","id":"1YCD","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P38777","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P38777","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"36481","properties":[{"key":"Interactions","value":"72"}]},{"database":"DIP","id":"DIP-5101N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P38777","properties":[{"key":"Number of interactors","value":"229"}]},{"database":"STRING","id":"4932.YHR049W","properties":[{"key":"Description","value":"-"}]},{"database":"ESTHER","id":"yeast-FSH1","properties":[{"key":"FamilyName","value":"FSH1"}]},{"database":"iPTMnet","id":"P38777","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YHR049W","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P38777","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YHR049W_mRNA","properties":[{"key":"ProteinId","value":"YHR049W"},{"key":"GeneId","value":"YHR049W"}]},{"database":"GeneID","id":"856445","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YHR049W","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000001091","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000001091","properties":[{"key":"GeneName","value":"FSH1"}]},{"database":"VEuPathDB","id":"FungiDB:YHR049W","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG2551","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_051938_2_3_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P38777","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"FEHPGGH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"2094269at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-31104-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"856445","properties":[{"key":"hits","value":"0 hits in 10 CRISPR screens"}]},{"database":"EvolutionaryTrace","id":"P38777","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P38777","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome VIII"}]},{"database":"RNAct","id":"P38777","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"14562095"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"14562095"}]},{"database":"GO","id":"GO:0016787","properties":[{"key":"GoTerm","value":"F:hydrolase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0016788","properties":[{"key":"GoTerm","value":"F:hydrolase activity, acting on ester bonds"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32182256"}]},{"database":"GO","id":"GO:0004622","properties":[{"key":"GoTerm","value":"F:phosphatidylcholine lysophospholipase A1 activity"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32920715"}]},{"database":"GO","id":"GO:0006915","properties":[{"key":"GoTerm","value":"P:apoptotic process"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"31363875"}]},{"database":"FunFam","id":"3.40.50.1820:FF:000279","properties":[{"key":"EntryName","value":"Family of serine hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1820","properties":[{"key":"EntryName","value":"alpha/beta hydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR029058","properties":[{"key":"EntryName","value":"AB_hydrolase_fold"}]},{"database":"InterPro","id":"IPR005645","properties":[{"key":"EntryName","value":"FSH-like_dom"}]},{"database":"InterPro","id":"IPR050593","properties":[{"key":"EntryName","value":"LovG"}]},{"database":"PANTHER","id":"PTHR48070","properties":[{"key":"EntryName","value":"ESTERASE OVCA2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR48070:SF9","properties":[{"key":"EntryName","value":"FAMILY OF SERINE HYDROLASES 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03959","properties":[{"key":"EntryName","value":"FSH1"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53474","properties":[{"key":"EntryName","value":"alpha/beta-Hydrolases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTVQIPKLLFLHGFLQNGKVFSEKSSGIRKLLKKANVQCDYIDAPVLLEKKDLPFEMDDEKWQATLDADVNRAWFYHSEISHELDISEGLKSVVDHIKANGPYDGIVGFSQGAALSSIITNKISELVPDHPQFKVSVVISGYSFTEPDPEHPGELRITEKFRDSFAVKPDMKTKMIFIYGASDQAVPSVRSKYLYDIYLKAQNGNKEKVLAYEHPGGHMVPNKKDIIRPIVEQITSSLQEASE","length":243,"molWeight":27339,"crc64":"C4B379487695426F","md5":"87F27FD7FBC38C42BC9C8F864DF42EDC"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":3,"Helix":11,"Beta strand":11,"Turn":3},"uniParcId":"UPI000013B2A5"}}