{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P38677","secondaryAccessions":["Q7RV80"],"uniProtkbId":"CMLE_NEUCR","entryAudit":{"firstPublicDate":"1995-02-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-01-23","entryVersion":135,"sequenceVersion":3},"annotationScore":4.0,"organism":{"scientificName":"Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)","taxonId":367110,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Sordariomycetes","Sordariomycetidae","Sordariales","Sordariaceae","Neurospora"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Carboxy-cis,cis-muconate cyclase"},"ecNumbers":[{"value":"5.5.1.5"}]},"alternativeNames":[{"fullName":{"value":"3-carboxy-cis,cis-muconate lactonizing enzyme"},"shortNames":[{"value":"CMLE"}]}]},"genes":[{"orfNames":[{"value":"NCU04071"}]}],"comments":[{"texts":[{"value":"Catalyzes a syn cycloisomerization. Also possesses mle activity"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 3-carboxy-cis,cis-muconate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:14977"},{"database":"ChEBI","id":"CHEBI:57496"},{"database":"ChEBI","id":"CHEBI:57976"}],"ecNumber":"5.5.1.5"}},{"texts":[{"value":"Aromatic compound metabolism; beta-ketoadipate pathway; 3-carboxy-cis,cis-muconate from 3-carboxy-2,5-dihydro-5-oxofuran-2-acetate: step 1/1"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11937053"}],"value":"Homotetramer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the cycloisomerase 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8132467"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":366,"modifier":"EXACT"}},"description":"Carboxy-cis,cis-muconate cyclase","featureId":"PRO_0000171127"},{"type":"Active site","location":{"start":{"value":149,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11937053"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1JOF"}]},{"type":"Active site","location":{"start":{"value":213,"modifier":"EXACT"},"end":{"value":213,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11937053"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1JOF"}]},{"type":"Site","location":{"start":{"value":197,"modifier":"EXACT"},"end":{"value":197,"modifier":"EXACT"}},"description":"Increases nucleophilicity of an active site residue","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11937053"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1JOF"}]},{"type":"Site","location":{"start":{"value":275,"modifier":"EXACT"},"end":{"value":275,"modifier":"EXACT"}},"description":"Increases nucleophilicity of an active site residue","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11937053"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":14,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Turn","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":52,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Helix","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":102,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":118,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":141,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":148,"modifier":"EXACT"},"end":{"value":153,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":157,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Turn","location":{"start":{"value":165,"modifier":"EXACT"},"end":{"value":168,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":169,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":181,"modifier":"EXACT"},"end":{"value":188,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":205,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Turn","location":{"start":{"value":213,"modifier":"EXACT"},"end":{"value":216,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":217,"modifier":"EXACT"},"end":{"value":223,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Turn","location":{"start":{"value":225,"modifier":"EXACT"},"end":{"value":227,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":230,"modifier":"EXACT"},"end":{"value":240,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Turn","location":{"start":{"value":249,"modifier":"EXACT"},"end":{"value":251,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":263,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":267,"modifier":"EXACT"},"end":{"value":278,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":284,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":296,"modifier":"EXACT"},"end":{"value":304,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":315,"modifier":"EXACT"},"end":{"value":317,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":324,"modifier":"EXACT"},"end":{"value":328,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":330,"modifier":"EXACT"},"end":{"value":332,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":334,"modifier":"EXACT"},"end":{"value":341,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":344,"modifier":"EXACT"},"end":{"value":352,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]},{"type":"Beta strand","location":{"start":{"value":358,"modifier":"EXACT"},"end":{"value":365,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1JOF"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0413","category":"Molecular function","name":"Isomerase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"8132467","citationType":"journal article","authors":["Mazur P.","Henzel W.J.","Mattoo S.","Kozarich J.W."],"citationCrossReferences":[{"database":"PubMed","id":"8132467"},{"database":"DOI","id":"10.1128/jb.176.6.1718-1728.1994"}],"title":"3-carboxy-cis,cis-muconate lactonizing enzyme from Neurospora crassa: an alternate cycloisomerase motif.","publicationDate":"1994","journal":"J. Bacteriol.","firstPage":"1718","lastPage":"1728","volume":"176"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","PROTEIN SEQUENCE OF 2-41","CHARACTERIZATION"],"referenceComments":[{"value":"ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"12712197","citationType":"journal article","authors":["Galagan J.E.","Calvo S.E.","Borkovich K.A.","Selker E.U.","Read N.D.","Jaffe D.B.","FitzHugh W.","Ma L.-J.","Smirnov S.","Purcell S.","Rehman B.","Elkins T.","Engels R.","Wang S.","Nielsen C.B.","Butler J.","Endrizzi M.","Qui D.","Ianakiev P.","Bell-Pedersen D.","Nelson M.A.","Werner-Washburne M.","Selitrennikoff C.P.","Kinsey J.A.","Braun E.L.","Zelter A.","Schulte U.","Kothe G.O.","Jedd G.","Mewes H.-W.","Staben C.","Marcotte E.","Greenberg D.","Roy A.","Foley K.","Naylor J.","Stange-Thomann N.","Barrett R.","Gnerre S.","Kamal M.","Kamvysselis M.","Mauceli E.W.","Bielke C.","Rudd S.","Frishman D.","Krystofova S.","Rasmussen C.","Metzenberg R.L.","Perkins D.D.","Kroken S.","Cogoni C.","Macino G.","Catcheside D.E.A.","Li W.","Pratt R.J.","Osmani S.A.","DeSouza C.P.C.","Glass N.L.","Orbach M.J.","Berglund J.A.","Voelker R.","Yarden O.","Plamann M.","Seiler S.","Dunlap J.C.","Radford A.","Aramayo R.","Natvig D.O.","Alex L.A.","Mannhaupt G.","Ebbole D.J.","Freitag M.","Paulsen I.","Sachs M.S.","Lander E.S.","Nusbaum C.","Birren B.W."],"citationCrossReferences":[{"database":"PubMed","id":"12712197"},{"database":"DOI","id":"10.1038/nature01554"}],"title":"The genome sequence of the filamentous fungus Neurospora crassa.","publicationDate":"2003","journal":"Nature","firstPage":"859","lastPage":"868","volume":"422"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"11937053","citationType":"journal article","authors":["Kajander T.","Merckel M.C.","Thompson A.","Deacon A.M.","Mazur P.","Kozarich J.W.","Goldman A."],"citationCrossReferences":[{"database":"PubMed","id":"11937053"},{"database":"DOI","id":"10.1016/s0969-2126(02)00744-x"}],"title":"The structure of Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme, a beta propeller cycloisomerase.","publicationDate":"2002","journal":"Structure","firstPage":"483","lastPage":"492","volume":"10"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)","FUNCTION","SUBUNIT","ACTIVE SITE"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"1JOF"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L27538","properties":[{"key":"ProteinId","value":"AAA21020.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"CM002241","properties":[{"key":"ProteinId","value":"EAA28450.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"A55525","properties":[{"key":"EntryName","value":"A55525"}]},{"database":"PDB","id":"1JOF","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.50 A"},{"key":"Chains","value":"A/B/C/D/E/F/G/H=2-366"}]},{"database":"PDBsum","id":"1JOF","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P38677","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P38677","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"367110.P38677","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"367110-P38677","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"EAA28450","properties":[{"key":"ProteinId","value":"EAA28450"},{"key":"GeneId","value":"NCU04071"}]},{"database":"KEGG","id":"ncr:NCU04071","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"FungiDB:NCU04071","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_045869_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P38677","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"VQNYEYQ","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"1715191at2759","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00157","properties":[{"key":"RectionId","value":"UER00308"}]},{"database":"EvolutionaryTrace","id":"P38677","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001805","properties":[{"key":"Component","value":"Chromosome 5, Linkage Group VI"}]},{"database":"GO","id":"GO:0017057","properties":[{"key":"GoTerm","value":"F:6-phosphogluconolactonase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0047768","properties":[{"key":"GoTerm","value":"F:carboxy-cis,cis-muconate cyclase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0042952","properties":[{"key":"GoTerm","value":"P:beta-ketoadipate pathway"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"FunFam","id":"2.130.10.10:FF:000244","properties":[{"key":"EntryName","value":"Carboxy-cis,cis-muconate cyclase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.130.10.10","properties":[{"key":"EntryName","value":"YVTN repeat-like/Quinoprotein amine dehydrogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050282","properties":[{"key":"EntryName","value":"Cycloisomerase_2"}]},{"database":"InterPro","id":"IPR019405","properties":[{"key":"EntryName","value":"Lactonase_7-beta_prop"}]},{"database":"InterPro","id":"IPR015943","properties":[{"key":"EntryName","value":"WD40/YVTN_repeat-like_dom_sf"}]},{"database":"PANTHER","id":"PTHR30344","properties":[{"key":"EntryName","value":"6-PHOSPHOGLUCONOLACTONASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30344:SF4","properties":[{"key":"EntryName","value":"CYCLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G11580)-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF10282","properties":[{"key":"EntryName","value":"Lactonase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF75011","properties":[{"key":"EntryName","value":"3-carboxy-cis,cis-mucoante lactonizing enzyme"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPLHHLMIGTWTPPGAIFTVQFDDEKLTCKLIKRTEIPQDEPISWMTFDHERKNIYGAAMKKWSSFAVKSPTEIVHEASHPIGGHPRANDADTNTRAIFLLAAKQPPYAVYANPFYKFAGYGNVFSVSETGKLEKNVQNYEYQENTGIHGMVFDPTETYLYSADLTANKLWTHRKLASGEVELVGSVDAPDPGDHPRWVAMHPTGNYLYALMEAGNRICEYVIDPATHMPVYTHHSFPLIPPGIPDRDPETGKGLYRADVCALTFSGKYMFASSRANKFELQGYIAGFKLRDCGSIEKQLFLSPTPTSGGHSNAVSPCPWSDEWMAITDDQEGWLEIYRWKDEFLHRVARVRIPEPGFGMNAIWYD","length":366,"molWeight":41329,"crc64":"E71BA50509E72873","md5":"3F297741E0EC639E12B6FD4C95D3F578"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Initiator methionine":1,"Chain":1,"Active site":2,"Site":2,"Beta strand":29,"Turn":5,"Helix":1},"uniParcId":"UPI000016892D"}}