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The cascade is composed of two c-di-GMP control modules, in which c-di-GMP controlled by the DgcE/PdeH pair (module I) regulates the activity of the DgcM/PdeR pair (module II), which in turn regulates activity of the transcription factor MlrA and expression of the master biofilm regulator csgD (PubMed:23708798)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 GTP = 3',3'-c-di-GMP + 2 diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24898"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:37565"},{"database":"ChEBI","id":"CHEBI:58805"}],"ecNumber":"2.7.7.65","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P31129"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P31129"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P31129"}],"value":"Binds 1 Mg(2+) ion per monomer."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31022167"}],"value":"Activated by the regulatory GTPase RdcA and its partner protein RdcB (PubMed:31022167). 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