{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P37661","secondaryAccessions":["Q2M7K7"],"uniProtkbId":"EPTB_ECOLI","entryAudit":{"firstPublicDate":"1994-10-01","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2001-12-19","entryVersion":167,"sequenceVersion":3},"annotationScore":4.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Kdo(2)-lipid A phosphoethanolamine 7''-transferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15795227"}],"value":"2.7.8.42"}]},"alternativeNames":[{"fullName":{"value":"Phosphoethanolamine transferase EptB"}}]},"genes":[{"geneName":{"value":"eptB"},"synonyms":[{"value":"yhjW"}],"orderedLocusNames":[{"value":"b3546"},{"value":"JW5660"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15795227"}],"value":"Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (Kdo) residue of a Kdo(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group function as pEtN donors and the reaction releases diacylglycerol"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A (E. coli) + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine = 7-O-[2-aminoethoxy(hydroxy)phosphoryl]-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A + a 1,2-diacyl-sn-glycerol","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24698"},{"database":"ChEBI","id":"CHEBI:17815"},{"database":"ChEBI","id":"CHEBI:58540"},{"database":"ChEBI","id":"CHEBI:60085"},{"database":"ChEBI","id":"CHEBI:64612"}],"ecNumber":"2.7.8.42","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15795227"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA (E. coli) + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine = 7-O-[2-aminoethoxy(hydroxy)phosphoryl]-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA (E. coli) + a 1,2-diacyl-sn-glycerol","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:46908"},{"database":"ChEBI","id":"CHEBI:17815"},{"database":"ChEBI","id":"CHEBI:60365"},{"database":"ChEBI","id":"CHEBI:64612"},{"database":"ChEBI","id":"CHEBI:87107"}],"ecNumber":"2.7.8.42","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15795227"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Ca(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15795227"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29108"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15795227"}],"value":"Inhibited by calcium concentrations higher than 1 mM"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15795227"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15795227"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the phosphoethanolamine transferase family. EptB subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":563,"modifier":"EXACT"}},"description":"Kdo(2)-lipid A phosphoethanolamine 7''-transferase","featureId":"PRO_0000209148"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":9,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":30,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":70,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":80,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":118,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":139,"modifier":"EXACT"},"end":{"value":159,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":160,"modifier":"EXACT"},"end":{"value":180,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":181,"modifier":"EXACT"},"end":{"value":563,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]}],"keywords":[{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0441","category":"Biological process","name":"Lipid A biosynthesis"},{"id":"KW-0444","category":"Biological process","name":"Lipid biosynthesis"},{"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"id":"KW-0448","category":"Biological process","name":"Lipopolysaccharide biosynthesis"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"8041620","citationType":"journal article","authors":["Sofia H.J.","Burland V.","Daniels D.L.","Plunkett G. III","Blattner F.R."],"citationCrossReferences":[{"database":"PubMed","id":"8041620"},{"database":"DOI","id":"10.1093/nar/22.13.2576"}],"title":"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.","publicationDate":"1994","journal":"Nucleic Acids Res.","firstPage":"2576","lastPage":"2586","volume":"22"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]","SEQUENCE REVISION TO C-TERMINUS"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"9298646","citationType":"journal article","authors":["Link A.J.","Robison K.","Church G.M."],"citationCrossReferences":[{"database":"PubMed","id":"9298646"},{"database":"DOI","id":"10.1002/elps.1150180807"}],"title":"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.","publicationDate":"1997","journal":"Electrophoresis","firstPage":"1259","lastPage":"1313","volume":"18"},"referencePositions":["PROTEIN SEQUENCE OF 220-231"],"referenceComments":[{"value":"K12 / EMG2","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"15795227","citationType":"journal article","authors":["Reynolds C.M.","Kalb S.R.","Cotter R.J.","Raetz C.R.H."],"citationCrossReferences":[{"database":"PubMed","id":"15795227"},{"database":"DOI","id":"10.1074/jbc.m500964200"}],"title":"A phosphoethanolamine transferase specific for the outer 3-deoxy-D-manno-octulosonic acid residue of Escherichia coli lipopolysaccharide. Identification of the eptB gene and Ca2+ hypersensitivity of an eptB deletion mutant.","publicationDate":"2005","journal":"J. Biol. Chem.","firstPage":"21202","lastPage":"21211","volume":"280"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","COFACTOR","ACTIVITY REGULATION","SUBCELLULAR LOCATION"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"15919996","citationType":"journal article","authors":["Daley D.O.","Rapp M.","Granseth E.","Melen K.","Drew D.","von Heijne G."],"citationCrossReferences":[{"database":"PubMed","id":"15919996"},{"database":"DOI","id":"10.1126/science.1109730"}],"title":"Global topology analysis of the Escherichia coli inner membrane proteome.","publicationDate":"2005","journal":"Science","firstPage":"1321","lastPage":"1323","volume":"308"},"referencePositions":["TOPOLOGY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U00039","properties":[{"key":"ProteinId","value":"AAB18523.1"},{"key":"Status","value":"ALT_FRAME"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC76570.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE77749.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"D65153","properties":[{"key":"EntryName","value":"D65153"}]},{"database":"RefSeq","id":"NP_418002.2","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001269197.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P37661","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P37661","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4262537","properties":[{"key":"Interactions","value":"8"}]},{"database":"BioGRID","id":"852376","properties":[{"key":"Interactions","value":"3"}]},{"database":"FunCoup","id":"P37661","properties":[{"key":"Number of interactors","value":"47"}]},{"database":"IntAct","id":"P37661","properties":[{"key":"Interactions","value":"5"}]},{"database":"STRING","id":"511145.b3546","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P37661","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b3546","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC76570","properties":[{"key":"ProteinId","value":"AAC76570"},{"key":"GeneId","value":"b3546"}]},{"database":"GeneID","id":"948068","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW5660","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b3546","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_19225","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.3168","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB2176","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG2194","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_018534_1_1_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P37661","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"FAMQSEV","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9786870at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P37661","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:EG12267-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:EG12267-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"2.7.8.42","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"PRO","id":"PR:P37661","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P37661","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15795227"}]},{"database":"GO","id":"GO:0043838","properties":[{"key":"GoTerm","value":"F:phosphatidylethanolamine:Kdo2-lipid A phosphoethanolamine transferase activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15795227"}]},{"database":"GO","id":"GO:0009245","properties":[{"key":"GoTerm","value":"P:lipid A biosynthetic process"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15795227"}]},{"database":"GO","id":"GO:0009244","properties":[{"key":"GoTerm","value":"P:lipopolysaccharide core region biosynthetic process"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd16017","properties":[{"key":"EntryName","value":"LptA"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.720.10:FF:000030","properties":[{"key":"EntryName","value":"Phosphoethanolamine transferase eptB"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.720.10","properties":[{"key":"EntryName","value":"Alkaline Phosphatase, subunit A"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR017850","properties":[{"key":"EntryName","value":"Alkaline_phosphatase_core_sf"}]},{"database":"InterPro","id":"IPR012549","properties":[{"key":"EntryName","value":"EptA-like_N"}]},{"database":"InterPro","id":"IPR058130","properties":[{"key":"EntryName","value":"PEA_transf_C"}]},{"database":"InterPro","id":"IPR040423","properties":[{"key":"EntryName","value":"PEA_transferase"}]},{"database":"InterPro","id":"IPR000917","properties":[{"key":"EntryName","value":"Sulfatase_N"}]},{"database":"NCBIfam","id":"NF008593","properties":[{"key":"EntryName","value":"PRK11560.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30443","properties":[{"key":"EntryName","value":"INNER MEMBRANE PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30443:SF3","properties":[{"key":"EntryName","value":"KDO(2)-LIPID A PHOSPHOETHANOLAMINE 7''-TRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08019","properties":[{"key":"EntryName","value":"EptA_B_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00884","properties":[{"key":"EntryName","value":"Sulfatase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53649","properties":[{"key":"EntryName","value":"Alkaline phosphatase-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWKGISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYGIIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRSLAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAWARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKLAQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDLYAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLIILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVIDQVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQAFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN","length":563,"molWeight":63804,"crc64":"40CF0E6A31779230","md5":"DF841A9ECB8BD49056C65C4EC0CAA48D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":1,"ACTIVITY REGULATION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":6,"Transmembrane":5},"uniParcId":"UPI000013B29D"}}