{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P37542","uniProtkbId":"YABA_BACSU","entryAudit":{"firstPublicDate":"1994-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1994-10-01","entryVersion":137,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01159"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12060778"}],"value":"Replication initiation control protein YabA"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01159"}],"value":"yabA"},"orderedLocusNames":[{"value":"BSU00330"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01159"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12060778"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15927750"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16461910"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18506095"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19081080"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21895792"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23909787"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28166228"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36053906"}],"value":"Involved in control of chromosome replication initiation. Inhibits the cooperative binding of DnaA to the oriC region (PubMed:21895792). Thus negatively regulating initiation of chromosome replication (PubMed:12060778, PubMed:15927750, PubMed:16461910, PubMed:18506095). Inhibits the ability of DnaA-ATP to form a helix on DNA; does not disassemble preformed DnaA-DNA helices in vitro (PubMed:23909787). Decreases the residence time of DnaA on the chromosome at its binding sites (oriC, replication forks and (probably) promoter-binding sites) (PubMed:28166228). Tethers DnaA to the replication machinery via the DNA polymerase beta sliding clamp subunit (dnaN) (PubMed:19081080). Associates with oriC and other DnaA targets on the chromosome in a DnaA-dependent manner (PubMed:21895792)"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01159"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26615189"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"16461910"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01159"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26615189"}],"value":"Binds one Zn(2+) ion per subunit (PubMed:26615189)."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12060778"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16461910"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18506095"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23909787"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26615189"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29619013"}],"value":"Homotetramer (PubMed:16461910). Interacts with both DnaA (PubMed:12060778, PubMed:16461910, PubMed:18506095, PubMed:23909787, PubMed:29619013) and DnaN, possibly acting as a bridge between these two proteins (PubMed:12060778, PubMed:16461910, PubMed:18506095, PubMed:29619013). The N-terminus (residues 1-62) assembles as a head-to-head dimer of dimers with a single long alpha helix, and is probably responsible for tetramerization of the whole protein (PubMed:26615189). The interaction sites for DnaA and DnaN overlap; thus DnaA and DnaN associate with different subunits of the YabA tetramer (PubMed:26615189). Interacts with AcuB and TlpA (PubMed:12060778, PubMed:16461910, PubMed:29619013)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P37542","intActId":"EBI-5243764"},"interactantTwo":{"uniProtKBAccession":"P05648","geneName":"dnaA","intActId":"EBI-2014722"},"numberOfExperiments":4,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P37542","intActId":"EBI-5243764"},"interactantTwo":{"uniProtKBAccession":"P05649","geneName":"dnaN","intActId":"EBI-5244587"},"numberOfExperiments":4,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P37542","intActId":"EBI-5243764"},"interactantTwo":{"uniProtKBAccession":"P37542","geneName":"yabA","intActId":"EBI-5243764"},"numberOfExperiments":6,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01159"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15927750"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16461910"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18506095"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19737352"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21170359"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26615189"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28166228"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29619013"}],"value":"Localizes in tight foci, most of which correspond to the replisome at mid-cell throughout the cell cycle (PubMed:15927750, PubMed:16461910, PubMed:19737352, PubMed:21170359, PubMed:26615189, PubMed:28166228, PubMed:29619013). When replication is blocked foci are also lost (PubMed:19737352). Correct positioning relies on interactions with both DnaA and DnaN, tested by mutagenesis (PubMed:16461910, PubMed:18506095). Positioning does not rely on DnaA, tested in a dnaA deletion strain (PubMed:19737352). Positioning does not rely on the C-terminus of SSB (ssbA) (PubMed:21170359)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01159"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15927750"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16461910"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19737352"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21170359"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26615189"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29619013"}],"value":"Cytoplasm, nucleoid","id":"SL-0187"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29619013"}],"value":"Constitutively transcribed (PubMed:29619013)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26615189"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"16461910"}],"value":"The N-terminus is probably responsible for self-association (Probable) (PubMed:16461910). The C-terminal domain (residues 62-119) interacts with DnaA or DnaN but not both at the same time (PubMed:26615189)"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29619013"}],"value":"Phosphorylation of YabA by YabT on Thr-71 enhances the cell's commitment to sporulation, possibly by increasing Spo0A-phosphorylation in cells (PubMed:29619013). The same phosphorylation event probably prevents biofilm formation (PubMed:29619013)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12060778"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15927750"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16461910"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19081080"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19737352"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21895792"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28166228"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29619013"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35576203"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36053906"}],"value":"Greatly reduced growth rate, abnormal nucleoid distribution with increased replication origins per cell and increased cell length (PubMed:12060778, PubMed:19737352, PubMed:36053906). Asynchronous and over initiation of DNA replication (PubMed:15927750, PubMed:16461910). DnaA is no longer tethered to the DNA replication machinery but instead to oriC, allowing it to initiate replication more often (PubMed:19081080). Increased half-life of DnaA binding to oriC (PubMed:28166228). Little or no effect on gene expression during exponetial growth or after replication arrest (PubMed:19737352). Increased amounts of DnaA associate with oriC and other tested DnaA boxes (PubMed:21895792). Significant decrease in heat-resistant spore formation, decrease in biofilm formation (PubMed:29619013). Sensitive to DNA damage (PubMed:35576203). A double temperature-sensitive dnaA1-yabA deletion is synthetically lethal at 37 degrees Celsius in rich medium, has severely impaired growth in minimal medium with greater replication initiation and SOS response induction (PubMed:36053906)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01159"}],"value":"Belongs to the YabA family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Replication initiation control protein YabA","featureId":"PRO_0000211906"},{"type":"Coiled coil","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26615189"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5DOL"}]},{"type":"Binding 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Decreased ability to disrupt DnaA oligomers.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16461910"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23909787"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["D"]}},{"type":"Mutagenesis","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":86,"modifier":"EXACT"}},"description":"Still self interacts, still interacts with DnaN, no longer interacts with DnaA, only residual localization.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16461910"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["P"]}},{"type":"Mutagenesis","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":94,"modifier":"EXACT"}},"description":"Still self interacts, still interacts with DnaN, no longer interacts with DnaA, only residual 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