{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P36434","uniProtkbId":"VSH2_HAEPA","entryAudit":{"firstPublicDate":"1994-06-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1994-06-01","entryVersion":84,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Haemophilus parainfluenzae","taxonId":729,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Pasteurellales","Pasteurellaceae","Haemophilus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12654995"}],"value":"Type II nicking enzyme V.HpaIIP"},"shortNames":[{"value":"V.HpaII"}],"ecNumbers":[{"value":"3.1.-.-"}]},"alternativeNames":[{"fullName":{"value":"HpaII very short patch repair endonuclease"}}]},"genes":[{"geneName":{"value":"vsr"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"7514149"}],"value":"hpaIIV"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P09184"}],"value":"May nick HpaII sequences that contain T/G mispairs resulting from m5C-deamination. If unrepaired, these mismatches can lead to C-to-T transition mutations. The very short patch (VSP) repair process counteracts the mutagenic process by repairing the mismatches in favor of the G-containing strand. This enzyme is an endonuclease that nicks double-stranded DNA within the sequence CTGG next to the thymidine residue that is mismatched to 2'-deoxyguanosine. The incision is mismatch-dependent and strand-specific"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the Vsr family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":141,"modifier":"EXACT"}},"description":"Type II nicking enzyme V.HpaIIP","featureId":"PRO_0000200290"}],"keywords":[{"id":"KW-0227","category":"Biological process","name":"DNA damage"},{"id":"KW-0234","category":"Biological process","name":"DNA repair"},{"id":"KW-0255","category":"Molecular function","name":"Endonuclease"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0540","category":"Molecular function","name":"Nuclease"},{"id":"KW-0680","category":"Biological process","name":"Restriction system"}],"references":[{"referenceNumber":1,"citation":{"id":"7514149","citationType":"journal article","authors":["Kulakauskas S.","Barsomian J.M.","Lubys A.","Roberts 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