{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P33753","secondaryAccessions":["D6VXB7","P30618","Q86ZR6","Q9HGQ5"],"uniProtkbId":"TRM2_YEAST","entryAudit":{"firstPublicDate":"1994-02-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2006-07-25","entryVersion":194,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"tRNA (uracil(54)-C(5))-methyltransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10864043"}],"value":"2.1.1.35"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"1408836"}],"value":"Rho-associated nuclease 1"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"7074684"}],"value":"Transfer RNA methyltransferase 2"}},{"fullName":{"value":"tRNA(m5U54)-methyltransferase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"7074684"}],"value":"TRM2"},"synonyms":[{"value":"NUC2"},{"value":"NUD1"},{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"1408836"}],"value":"RNC1"}],"orderedLocusNames":[{"value":"YKR056W"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10864043"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12003492"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17205207"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37181630"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7074684"}],"value":"Catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA (PubMed:10864043, PubMed:12003492, PubMed:17205207, PubMed:7074684). May also have a role in tRNA stabilization or maturation (PubMed:12003492). Also involved in the formation of 5-methyl-uridine in mRNA, which alters the elongation step in protein synthesis in a position-dependent manner, slowing amino acid addition by the ribosome (PubMed:37181630). Displays 5'-3' exonuclease activity on double-strand (ds) DNA, and endonuclease activity on single-strand (ss) DNA, characteristic of endo-exonucleases. May be involved in DNA double-strand break repair (PubMed:17205207)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"uridine(54) in tRNA + S-adenosyl-L-methionine = 5-methyluridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42712"},{"database":"Rhea","id":"RHEA-COMP:10167"},{"database":"Rhea","id":"RHEA-COMP:10193"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:65315"},{"database":"ChEBI","id":"CHEBI:74447"}],"ecNumber":"2.1.1.35","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10864043"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01024"}],"value":"Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"It is uncertain whether Met-1, Met-38, Met-71, Met-77 or Met-82 is the initiator"}],"commentType":"CAUTION"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous gene model prediction","sequence":"CAA78500.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Miscellaneous discrepancy","sequence":"CAA78500.1","note":"Cloning artifact which fused this protein with RHO4.","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"CAA81021.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"CAA82134.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"CAC01942.1","note":"Truncated N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":639,"modifier":"EXACT"}},"description":"tRNA (uracil(54)-C(5))-methyltransferase","featureId":"PRO_0000162059"},{"type":"Domain","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":228,"modifier":"EXACT"}},"description":"TRAM","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00208"}]},{"type":"Region","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"Basic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":591,"modifier":"EXACT"},"end":{"value":591,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01024"}]},{"type":"Active site","location":{"start":{"value":631,"modifier":"EXACT"},"end":{"value":631,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10015"}]},{"type":"Binding site","location":{"start":{"value":461,"modifier":"EXACT"},"end":{"value":461,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01024"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":496,"modifier":"EXACT"},"end":{"value":496,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01024"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":517,"modifier":"EXACT"},"end":{"value":517,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01024"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":564,"modifier":"EXACT"},"end":{"value":564,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01024"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Modified residue","location":{"start":{"value":92,"modifier":"EXACT"},"end":{"value":92,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17330950"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":93,"modifier":"EXACT"},"end":{"value":93,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17330950"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Mutagenesis","location":{"start":{"value":498,"modifier":"EXACT"},"end":{"value":498,"modifier":"EXACT"}},"description":"Unable to methylate tRNA.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12003492"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["D"]}},{"type":"Mutagenesis","location":{"start":{"value":591,"modifier":"EXACT"},"end":{"value":591,"modifier":"EXACT"}},"description":"Unable to methylate tRNA.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12003492"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"in Ref. 5; CAA78500","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["Y"]}}],"keywords":[{"id":"KW-0489","category":"Molecular function","name":"Methyltransferase"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0949","category":"Ligand","name":"S-adenosyl-L-methionine"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0819","category":"Biological process","name":"tRNA processing"}],"references":[{"referenceNumber":1,"citation":{"id":"8256523","citationType":"journal article","authors":["van Vliet-Reedijk J.C.","Planta R.J."],"citationCrossReferences":[{"database":"PubMed","id":"8256523"},{"database":"DOI","id":"10.1002/yea.320091015"}],"title":"The RHO4a and NUD1 genes on Saccharomyces cerevisiae chromosome XI.","publicationDate":"1993","journal":"Yeast","firstPage":"1139","lastPage":"1147","volume":"9"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"10864043","citationType":"journal article","authors":["Nordlund M.E.","Johansson J.O.M.","Von Pawel-Rammingen U.","Bystroem A.S."],"citationCrossReferences":[{"database":"PubMed","id":"10864043"},{"database":"DOI","id":"10.1017/s1355838200992422"}],"title":"Identification of the TRM2 gene encoding the tRNA(m5U54)methyltransferase of Saccharomyces cerevisiae.","publicationDate":"2000","journal":"RNA","firstPage":"844","lastPage":"860","volume":"6"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","IDENTIFICATION OF FRAMESHIFT","FUNCTION","CATALYTIC ACTIVITY"]},{"referenceNumber":3,"citation":{"id":"8196765","citationType":"journal article","authors":["Dujon B.","Alexandraki D.","Andre B.","Ansorge W.","Baladron V.","Ballesta J.P.G.","Banrevi A.","Bolle P.-A.","Bolotin-Fukuhara M.","Bossier P.","Bou G.","Boyer J.","Buitrago M.J.","Cheret G.","Colleaux L.","Daignan-Fornier B.","del Rey F.","Dion C.","Domdey H.","Duesterhoeft A.","Duesterhus S.","Entian K.-D.","Erfle H.","Esteban P.F.","Feldmann H.","Fernandes L.","Fobo G.M.","Fritz C.","Fukuhara H.","Gabel C.","Gaillon L.","Garcia-Cantalejo J.M.","Garcia-Ramirez J.J.","Gent M.E.","Ghazvini M.","Goffeau A.","Gonzalez A.","Grothues D.","Guerreiro P.","Hegemann J.H.","Hewitt N.","Hilger F.","Hollenberg C.P.","Horaitis O.","Indge K.J.","Jacquier A.","James C.M.","Jauniaux J.-C.","Jimenez A.","Keuchel H.","Kirchrath L.","Kleine K.","Koetter P.","Legrain P.","Liebl S.","Louis E.J.","Maia e Silva A.","Marck C.","Monnier A.-L.","Moestl D.","Mueller S.","Obermaier B.","Oliver S.G.","Pallier C.","Pascolo S.","Pfeiffer F.","Philippsen P.","Planta R.J.","Pohl F.M.","Pohl T.M.","Poehlmann R.","Portetelle D.","Purnelle B.","Puzos V.","Ramezani Rad M.","Rasmussen S.W.","Remacha M.A.","Revuelta J.L.","Richard G.-F.","Rieger M.","Rodrigues-Pousada C.","Rose M.","Rupp T.","Santos M.A.","Schwager C.","Sensen C.","Skala J.","Soares H.","Sor F.","Stegemann J.","Tettelin H.","Thierry A.","Tzermia M.","Urrestarazu L.A.","van Dyck L.","van Vliet-Reedijk J.C.","Valens M.","Vandenbol M.","Vilela C.","Vissers S.","von Wettstein D.","Voss H.","Wiemann S.","Xu G.","Zimmermann J.","Haasemann M.","Becker I.","Mewes H.-W."],"citationCrossReferences":[{"database":"PubMed","id":"8196765"},{"database":"DOI","id":"10.1038/369371a0"}],"title":"Complete DNA sequence of yeast chromosome XI.","publicationDate":"1994","journal":"Nature","firstPage":"371","lastPage":"378","volume":"369"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"1408836","citationType":"journal article","authors":["Chow T.Y.-K.","Perkins E.L.","Resnick M.A."],"citationCrossReferences":[{"database":"PubMed","id":"1408836"},{"database":"DOI","id":"10.1093/nar/20.19.5215"}],"title":"Yeast RNC1 encodes a chimeric protein, RhoNUC, with a human rho motif and deoxyribonuclease activity.","publicationDate":"1992","journal":"Nucleic Acids Res.","firstPage":"5215","lastPage":"5221","volume":"20"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-168"]},{"referenceNumber":6,"citation":{"id":"12844361","citationType":"journal article","authors":["Brachat S.","Dietrich F.S.","Voegeli S.","Zhang Z.","Stuart L.","Lerch A.","Gates K.","Gaffney T.D.","Philippsen P."],"citationCrossReferences":[{"database":"PubMed","id":"12844361"},{"database":"DOI","id":"10.1186/gb-2003-4-7-r45"}],"title":"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii.","publicationDate":"2003","journal":"Genome Biol.","firstPage":"R45.1","lastPage":"R45.13","volume":"4"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 552-628","IDENTIFICATION OF FRAMESHIFT"],"referenceComments":[{"value":"ATCC 204511 / S288c / AB972","type":"STRAIN"}]},{"referenceNumber":7,"citation":{"id":"7074684","citationType":"journal article","authors":["Hopper A.K.","Furukawa A.H.","Pham H.D.","Martin N.C."],"citationCrossReferences":[{"database":"PubMed","id":"7074684"},{"database":"DOI","id":"10.1016/0092-8674(82)90209-4"}],"title":"Defects in modification of cytoplasmic and mitochondrial transfer RNAs are caused by single nuclear mutations.","publicationDate":"1982","journal":"Cell","firstPage":"543","lastPage":"550","volume":"28"},"referencePositions":["FUNCTION"]},{"referenceNumber":8,"citation":{"id":"12003492","citationType":"journal article","authors":["Johansson M.J.","Bystroem A.S."],"citationCrossReferences":[{"database":"PubMed","id":"12003492"},{"database":"DOI","id":"10.1017/s1355838202027851"}],"title":"Dual function of the tRNA(m(5)U54)methyltransferase in tRNA maturation.","publicationDate":"2002","journal":"RNA","firstPage":"324","lastPage":"335","volume":"8"},"referencePositions":["FUNCTION","MUTAGENESIS OF GLY-498 AND CYS-591"]},{"referenceNumber":9,"citation":{"id":"17330950","citationType":"journal article","authors":["Li X.","Gerber S.A.","Rudner A.D.","Beausoleil S.A.","Haas W.","Villen J.","Elias J.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"17330950"},{"database":"DOI","id":"10.1021/pr060559j"}],"title":"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.","publicationDate":"2007","journal":"J. Proteome Res.","firstPage":"1190","lastPage":"1197","volume":"6"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92 AND SER-93","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"ADR376","type":"STRAIN"}]},{"referenceNumber":10,"citation":{"id":"17205207","citationType":"journal article","authors":["Choudhury S.A.","Asefa B.","Webb A.","Ramotar D.","Chow T.Y."],"citationCrossReferences":[{"database":"PubMed","id":"17205207"},{"database":"DOI","id":"10.1007/s11010-006-9386-1"}],"title":"Functional and genetic analysis of the Saccharomyces cerevisiae RNC1/TRM2: evidences for its involvement in DNA double-strand break repair.","publicationDate":"2007","journal":"Mol. Cell. Biochem.","firstPage":"215","lastPage":"226","volume":"300"},"referencePositions":["FUNCTION"]},{"referenceNumber":11,"citation":{"id":"17287358","citationType":"journal article","authors":["Chi A.","Huttenhower C.","Geer L.Y.","Coon J.J.","Syka J.E.P.","Bai D.L.","Shabanowitz J.","Burke D.J.","Troyanskaya O.G.","Hunt D.F."],"citationCrossReferences":[{"database":"PubMed","id":"17287358"},{"database":"DOI","id":"10.1073/pnas.0607084104"}],"title":"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.","publicationDate":"2007","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"2193","lastPage":"2198","volume":"104"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":12,"citation":{"id":"18407956","citationType":"journal article","authors":["Albuquerque C.P.","Smolka M.B.","Payne S.H.","Bafna V.","Eng J.","Zhou H."],"citationCrossReferences":[{"database":"PubMed","id":"18407956"},{"database":"DOI","id":"10.1074/mcp.m700468-mcp200"}],"title":"A multidimensional chromatography technology for in-depth phosphoproteome analysis.","publicationDate":"2008","journal":"Mol. Cell. Proteomics","firstPage":"1389","lastPage":"1396","volume":"7"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":13,"citation":{"id":"19779198","citationType":"journal article","authors":["Holt L.J.","Tuch B.B.","Villen J.","Johnson A.D.","Gygi S.P.","Morgan D.O."],"citationCrossReferences":[{"database":"PubMed","id":"19779198"},{"database":"DOI","id":"10.1126/science.1172867"}],"title":"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.","publicationDate":"2009","journal":"Science","firstPage":"1682","lastPage":"1686","volume":"325"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92 AND SER-93","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":14,"citation":{"id":"37181630","citationType":"journal article","authors":["Jones J.D.","Franco M.K.","Smith T.J.","Snyder L.R.","Anders A.G.","Ruotolo B.T.","Kennedy R.T.","Koutmou K.S."],"citationCrossReferences":[{"database":"PubMed","id":"37181630"},{"database":"DOI","id":"10.1039/d2cb00229a"}],"title":"Methylated guanosine and uridine modifications in S. cerevisiae mRNAs modulate translation elongation.","publicationDate":"2023","journal":"RSC Chem. Biol.","firstPage":"363","lastPage":"378","volume":"4"},"referencePositions":["FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"Z25734","properties":[{"key":"ProteinId","value":"CAA81021.1"},{"key":"Status","value":"ALT_FRAME"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AJ250970","properties":[{"key":"ProteinId","value":"CAC01942.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"Z28281","properties":[{"key":"ProteinId","value":"CAA82134.1"},{"key":"Status","value":"ALT_FRAME"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"Z14126","properties":[{"key":"ProteinId","value":"CAA78500.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY260897","properties":[{"key":"ProteinId","value":"AAP21765.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006944","properties":[{"key":"ProteinId","value":"DAA09207.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S37744","properties":[{"key":"EntryName","value":"S37744"}]},{"database":"RefSeq","id":"NP_012982.2","properties":[{"key":"NucleotideSequenceId","value":"NM_001179846.1"}]},{"database":"AlphaFoldDB","id":"P33753","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P33753","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"34187","properties":[{"key":"Interactions","value":"108"}]},{"database":"FunCoup","id":"P33753","properties":[{"key":"Number of interactors","value":"343"}]},{"database":"MINT","id":"P33753","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"4932.YKR056W","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"P33753","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YKR056W","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P33753","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YKR056W_mRNA","properties":[{"key":"ProteinId","value":"YKR056W"},{"key":"GeneId","value":"YKR056W"}]},{"database":"GeneID","id":"853930","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YKR056W","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000001764","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000001764","properties":[{"key":"GeneName","value":"TRM2"}]},{"database":"VEuPathDB","id":"FungiDB:YKR056W","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG2187","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_014689_3_2_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P33753","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"GGCKWQH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"10250660at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-32025-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"853930","properties":[{"key":"hits","value":"9 hits in 10 CRISPR screens"}]},{"database":"PRO","id":"PR:P33753","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome XI"}]},{"database":"RNAct","id":"P33753","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0051908","properties":[{"key":"GoTerm","value":"F:double-stranded DNA 5'-3' DNA exonuclease activity"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17205207"}]},{"database":"GO","id":"GO:0000014","properties":[{"key":"GoTerm","value":"F:single-stranded DNA endonuclease activity"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17205207"}]},{"database":"GO","id":"GO:0030697","properties":[{"key":"GoTerm","value":"F:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"10864043"}]},{"database":"GO","id":"GO:0006400","properties":[{"key":"GoTerm","value":"P:tRNA modification"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"10864043"}]},{"database":"CDD","id":"cd02440","properties":[{"key":"EntryName","value":"AdoMet_MTases"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.50.140:FF:000201","properties":[{"key":"EntryName","value":"TRM2p tRNA methyltransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.150:FF:000174","properties":[{"key":"EntryName","value":"TRM2p tRNA methyltransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.50.1070","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.50.140","properties":[{"key":"EntryName","value":"Nucleic acid-binding proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.150","properties":[{"key":"EntryName","value":"Vaccinia Virus protein VP39"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR030390","properties":[{"key":"EntryName","value":"MeTrfase_TrmA_AS"}]},{"database":"InterPro","id":"IPR030391","properties":[{"key":"EntryName","value":"MeTrfase_TrmA_CS"}]},{"database":"InterPro","id":"IPR012340","properties":[{"key":"EntryName","value":"NA-bd_OB-fold"}]},{"database":"InterPro","id":"IPR029063","properties":[{"key":"EntryName","value":"SAM-dependent_MTases_sf"}]},{"database":"InterPro","id":"IPR002792","properties":[{"key":"EntryName","value":"TRAM_dom"}]},{"database":"InterPro","id":"IPR025795","properties":[{"key":"EntryName","value":"tRNA_(uracil-5-)_MeTrfase"}]},{"database":"InterPro","id":"IPR010280","properties":[{"key":"EntryName","value":"U5_MeTrfase_fam"}]},{"database":"PANTHER","id":"PTHR11061","properties":[{"key":"EntryName","value":"RNA M5U METHYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11061:SF30","properties":[{"key":"EntryName","value":"TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01938","properties":[{"key":"EntryName","value":"TRAM"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05958","properties":[{"key":"EntryName","value":"tRNA_U5-meth_tr"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50249","properties":[{"key":"EntryName","value":"Nucleic acid-binding proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53335","properties":[{"key":"EntryName","value":"S-adenosyl-L-methionine-dependent methyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51687","properties":[{"key":"EntryName","value":"SAM_MT_RNA_M5U"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51622","properties":[{"key":"EntryName","value":"SAM_MT_RNA_M5U_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50926","properties":[{"key":"EntryName","value":"TRAM"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01230","properties":[{"key":"EntryName","value":"TRMA_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01231","properties":[{"key":"EntryName","value":"TRMA_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MYEQFEFSFFFFENSDNKVKYKAHLISSIKRWSIITCMRCFWTVQKSIFKARFFACRNFVKKHNYKLISTMTGSTEMVPPTMKHTVDNKRLSSPLTDSGNRRTKKPKLRKYKAKKVETTSPMGVLEFEVNDLLKSQNLSREQVLNDVTSILNDKSSTDGPIVLQYHREVKNVKVLEITSNGNGLALIDNPVETEKKQVVIIPFGLPGDVVNIKVFKTHPYYVESDLLDVVEKSPMRRDDLIRDKYFGKSSGSQLEFLTYDDQLELKRKTIMNAYKFFAPRLVAEKLLPPFDTTVASPLQFGYRTKITPHFDMPKRKQKELSVRPPLGFGQKGRPQWRKDTLDIGGHGSILDIDECVLATEVLNKGLTNERRKFEQEFKNYKKGATILLRENTTILDPSKPTLEQLTEEASRDENGDISYVEVEDKKNNVRLAKTCVTNPRQIVTEYVDGYTFNFSAGEFFQNNNSILPIVTKYVRDNLQAPAKGDDNKTKFLVDAYCGSGLFSICSSKGVDKVIGVEISADSVSFAEKNAKANGVENCRFIVGKAEKLFESIDTPSENTSVILDPPRKGCDELFLKQLAAYNPAKIIYISCNVHSQARDVEYFLKETENGSAHQIESIRGFDFFPQTHHVESVCIMKRI","length":639,"molWeight":72852,"crc64":"E7EC5B1378F7C361","md5":"EA6397A1AAB1E24DDFE531BB4AEF08D3"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SIMILARITY":1,"CAUTION":1,"SEQUENCE CAUTION":5},"countByFeatureType":{"Chain":1,"Domain":1,"Region":1,"Compositional bias":1,"Active site":2,"Binding site":4,"Modified residue":2,"Mutagenesis":2,"Sequence conflict":1},"uniParcId":"UPI000034F5D2"}}