{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P33607","secondaryAccessions":["P78254"],"uniProtkbId":"NUOL_ECOLI","entryAudit":{"firstPublicDate":"1994-02-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-11-01","entryVersion":168,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"NADH-quinone oxidoreductase subunit L"},"ecNumbers":[{"value":"7.1.1.-"}]},"alternativeNames":[{"fullName":{"value":"NADH dehydrogenase I subunit L"}},{"fullName":{"value":"NDH-1 subunit L"}},{"fullName":{"value":"NUO12"}}]},"genes":[{"geneName":{"value":"nuoL"},"orderedLocusNames":[{"value":"b2278"},{"value":"JW2273"}]}],"comments":[{"texts":[{"value":"NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a quinone + NADH + 5 H(+)(in) = a quinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:57888"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:24646"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:132124"}]}},{"texts":[{"value":"Composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Cell inner membrane","id":"SL-0037"},"topology":{"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the complex I subunit 5 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":613,"modifier":"EXACT"}},"description":"NADH-quinone oxidoreductase subunit L","featureId":"PRO_0000118213"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":6,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":31,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":53,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":100,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":116,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":134,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":228,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":229,"modifier":"EXACT"},"end":{"value":248,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":249,"modifier":"EXACT"},"end":{"value":267,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":268,"modifier":"EXACT"},"end":{"value":281,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":282,"modifier":"EXACT"},"end":{"value":300,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":306,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":307,"modifier":"EXACT"},"end":{"value":325,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":326,"modifier":"EXACT"},"end":{"value":338,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":339,"modifier":"EXACT"},"end":{"value":356,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":357,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":374,"modifier":"EXACT"},"end":{"value":397,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":398,"modifier":"EXACT"},"end":{"value":413,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":414,"modifier":"EXACT"},"end":{"value":437,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":438,"modifier":"EXACT"},"end":{"value":454,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":455,"modifier":"EXACT"},"end":{"value":472,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":473,"modifier":"EXACT"},"end":{"value":494,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":495,"modifier":"EXACT"},"end":{"value":514,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":515,"modifier":"EXACT"},"end":{"value":589,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":590,"modifier":"EXACT"},"end":{"value":607,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":608,"modifier":"EXACT"},"end":{"value":613,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Sequence conflict","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"in Ref. 1; CAA48371","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"in Ref. 1; CAA48371","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"FIGVDFFANGEQ","alternativeSequences":["LSALISSLTASK"]}},{"type":"Sequence conflict","location":{"start":{"value":182,"modifier":"EXACT"},"end":{"value":182,"modifier":"EXACT"}},"description":"in Ref. 1; CAA48371","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["R"]}},{"type":"Sequence conflict","location":{"start":{"value":208,"modifier":"EXACT"},"end":{"value":208,"modifier":"EXACT"}},"description":"in Ref. 1; CAA48371","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["L"]}},{"type":"Turn","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":5,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Turn","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":57,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7S"}]},{"type":"Beta strand","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Beta strand","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":106,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Turn","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Beta strand","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":154,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":192,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":197,"modifier":"EXACT"},"end":{"value":207,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Beta strand","location":{"start":{"value":208,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z80"}]},{"type":"Helix","location":{"start":{"value":213,"modifier":"EXACT"},"end":{"value":230,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Beta strand","location":{"start":{"value":233,"modifier":"EXACT"},"end":{"value":235,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7P61"}]},{"type":"Helix","location":{"start":{"value":239,"modifier":"EXACT"},"end":{"value":242,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":247,"modifier":"EXACT"},"end":{"value":251,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":256,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":258,"modifier":"EXACT"},"end":{"value":260,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":261,"modifier":"EXACT"},"end":{"value":268,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":270,"modifier":"EXACT"},"end":{"value":274,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":277,"modifier":"EXACT"},"end":{"value":299,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":304,"modifier":"EXACT"},"end":{"value":324,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":328,"modifier":"EXACT"},"end":{"value":355,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Beta strand","location":{"start":{"value":356,"modifier":"EXACT"},"end":{"value":359,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Turn","location":{"start":{"value":362,"modifier":"EXACT"},"end":{"value":364,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Turn","location":{"start":{"value":369,"modifier":"EXACT"},"end":{"value":371,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":373,"modifier":"EXACT"},"end":{"value":386,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Turn","location":{"start":{"value":390,"modifier":"EXACT"},"end":{"value":392,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":394,"modifier":"EXACT"},"end":{"value":408,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":412,"modifier":"EXACT"},"end":{"value":437,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":454,"modifier":"EXACT"},"end":{"value":463,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":466,"modifier":"EXACT"},"end":{"value":470,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Turn","location":{"start":{"value":476,"modifier":"EXACT"},"end":{"value":478,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":480,"modifier":"EXACT"},"end":{"value":486,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":487,"modifier":"EXACT"},"end":{"value":512,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Beta strand","location":{"start":{"value":513,"modifier":"EXACT"},"end":{"value":515,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7ZCI"}]},{"type":"Helix","location":{"start":{"value":517,"modifier":"EXACT"},"end":{"value":524,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":526,"modifier":"EXACT"},"end":{"value":536,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":538,"modifier":"EXACT"},"end":{"value":540,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":541,"modifier":"EXACT"},"end":{"value":545,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":546,"modifier":"EXACT"},"end":{"value":550,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":551,"modifier":"EXACT"},"end":{"value":560,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":564,"modifier":"EXACT"},"end":{"value":583,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":584,"modifier":"EXACT"},"end":{"value":586,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":591,"modifier":"EXACT"},"end":{"value":611,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0520","category":"Ligand","name":"NAD"},{"id":"KW-0874","category":"PTM","name":"Quinone"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"7690854","citationType":"journal article","authors":["Weidner U.","Geier S.","Ptock A.","Friedrich T.","Leif H.","Weiss H."],"citationCrossReferences":[{"database":"PubMed","id":"7690854"},{"database":"DOI","id":"10.1006/jmbi.1993.1488"}],"title":"The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I.","publicationDate":"1993","journal":"J. Mol. Biol.","firstPage":"109","lastPage":"122","volume":"233"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"K12 / AN387","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9205837","citationType":"journal article","authors":["Yamamoto Y.","Aiba H.","Baba T.","Hayashi K.","Inada T.","Isono K.","Itoh T.","Kimura S.","Kitagawa M.","Makino K.","Miki T.","Mitsuhashi N.","Mizobuchi K.","Mori H.","Nakade S.","Nakamura Y.","Nashimoto H.","Oshima T.","Oyama S.","Saito N.","Sampei G.","Satoh Y.","Sivasundaram S.","Tagami H.","Takahashi H.","Takeda J.","Takemoto K.","Uehara K.","Wada C.","Yamagata S.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9205837"},{"database":"DOI","id":"10.1093/dnares/4.2.91"}],"title":"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.","publicationDate":"1997","journal":"DNA Res.","firstPage":"91","lastPage":"113","volume":"4"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"15919996","citationType":"journal article","authors":["Daley D.O.","Rapp M.","Granseth E.","Melen K.","Drew D.","von Heijne G."],"citationCrossReferences":[{"database":"PubMed","id":"15919996"},{"database":"DOI","id":"10.1126/science.1109730"}],"title":"Global topology analysis of the Escherichia coli inner membrane proteome.","publicationDate":"2005","journal":"Science","firstPage":"1321","lastPage":"1323","volume":"308"},"referencePositions":["TOPOLOGY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X68301","properties":[{"key":"ProteinId","value":"CAA48371.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC75338.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA16106.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"D64999","properties":[{"key":"EntryName","value":"D64999"}]},{"database":"RefSeq","id":"NP_416781.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001056643.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"PDB","id":"7NYH","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.60 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7NYR","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.30 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7NYU","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.80 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7NYV","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.70 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7P61","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.20 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7P62","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.60 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7P63","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.40 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7P64","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.50 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7P69","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7P7C","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.40 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7P7E","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7P7J","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7P7K","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.10 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7P7L","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7P7M","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.20 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7Z7R","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.36 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7Z7S","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.40 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7Z7T","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.10 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7Z7V","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.29 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7Z80","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.93 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7Z83","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.88 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7Z84","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.87 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7ZC5","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"7ZCI","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.69 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"9Q8I","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.35 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"9TAJ","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"9TAK","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.80 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"9TAM","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.96 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDB","id":"9TAO","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.61 A"},{"key":"Chains","value":"L=1-613"}]},{"database":"PDBsum","id":"7NYH","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7NYR","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7NYU","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7NYV","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P61","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P62","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P63","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P64","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P69","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7C","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7E","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7J","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7K","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7L","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7M","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7R","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7S","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7T","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7V","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z80","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z83","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z84","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7ZC5","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7ZCI","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9Q8I","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAJ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAK","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAM","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAO","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P33607","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-52895","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P33607","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4260888","properties":[{"key":"Interactions","value":"94"}]},{"database":"ComplexPortal","id":"CPX-243","properties":[{"key":"EntryName","value":"Respiratory chain complex I"}]},{"database":"DIP","id":"DIP-10388N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P33607","properties":[{"key":"Number of interactors","value":"345"}]},{"database":"IntAct","id":"P33607","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"511145.b2278","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"3.D.1.1.1","properties":[{"key":"FamilyName","value":"the h+ or na+-translocating nadh dehydrogenase (ndh) family"}]},{"database":"jPOST","id":"P33607","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b2278","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC75338","properties":[{"key":"ProteinId","value":"AAC75338"},{"key":"GeneId","value":"b2278"}]},{"database":"GeneID","id":"945540","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW2273","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b2278","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_12715","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.4458","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB2016","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG1009","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_007100_6_2_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P33607","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"LIGFWQH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9811798at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P33607","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:NUOL-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:NUOL-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P33607","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P33607","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16079137"}]},{"database":"GO","id":"GO:0030964","properties":[{"key":"GoTerm","value":"C:NADH dehydrogenase complex"},{"key":"GoEvidenceType","value":"IDA:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"7607227"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15919996"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17309111"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"7607227"}]},{"database":"GO","id":"GO:0045271","properties":[{"key":"GoTerm","value":"C:respiratory chain complex I"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17722886"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0042773","properties":[{"key":"GoTerm","value":"P:ATP synthesis coupled electron transport"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0015990","properties":[{"key":"GoTerm","value":"P:electron transport coupled proton transport"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"21417432"}]},{"database":"GO","id":"GO:0022904","properties":[{"key":"GoTerm","value":"P:respiratory electron transport chain"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18316732"}]},{"database":"FunFam","id":"1.20.5.2700:FF:000001","properties":[{"key":"EntryName","value":"NADH-quinone oxidoreductase, L subunit"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.5.2700","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR018393","properties":[{"key":"EntryName","value":"NADHpl_OxRdtase_5_subgr"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"InterPro","id":"IPR003945","properties":[{"key":"EntryName","value":"NU5C-like"}]},{"database":"InterPro","id":"IPR001516","properties":[{"key":"EntryName","value":"Proton_antipo_N"}]},{"database":"NCBIfam","id":"TIGR01974","properties":[{"key":"EntryName","value":"NDH_I_L"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF005141","properties":[{"key":"EntryName","value":"PRK06590.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42829","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42829:SF2","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00662","properties":[{"key":"EntryName","value":"Proton_antipo_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01434","properties":[{"key":"EntryName","value":"NADHDHGNASE5"}]},{"database":"PRINTS","id":"PR01435","properties":[{"key":"EntryName","value":"NPOXDRDTASE5"}]}],"sequence":{"value":"MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALVTAFIGVDFFANGEQTYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMYASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTDPKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWATLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMTPEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFHLMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLVTAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKGVTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLAAWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLNSMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR","length":613,"molWeight":66438,"crc64":"7B768F6D4F2668A1","md5":"8186956538AFCCC908488F644A376133"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":15,"Transmembrane":14,"Sequence conflict":4,"Turn":7,"Helix":33,"Beta strand":7},"uniParcId":"UPI0000130A14"}}