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This region of DNA acts as an antitoxin to toxin RalR, a DNase, but it seems to be sRNA RalA that has the antitoxin activity and not this putative protein. Therefore the identity of this as a protein-coding gene has been cast into doubt (PubMed:24748661)"}],"commentType":"FUNCTION"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P33230","intActId":"EBI-562744"},"interactantTwo":{"uniProtKBAccession":"P75728","geneName":"ubiF","intActId":"EBI-562588"},"numberOfExperiments":2,"organismDiffer":false}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22343303"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24748661"}],"value":"Mutants show increased numbers of double-strand breaks (PubMed:22343303). The single ralR and double ralR-ralA mutant have increased sensitivity to the antibiotic fosfomycin"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"Double-strand break reduction protein","featureId":"PRO_0000168901"},{"type":"Mutagenesis","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"No protein should be translated, DNA region still acts as an antitoxin to RalR.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24748661"}],"alternativeSequence":{"originalSequence":"M","alternativeSequences":["T"]}},{"type":"Mutagenesis","location":{"start":{"value":9,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"No protein should be translated, DNA region still acts as an antitoxin to RalR.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24748661"}],"alternativeSequence":{}}],"keywords":[{"id":"KW-0046","category":"Biological process","name":"Antibiotic resistance"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"9097039","citationType":"journal article","authors":["Aiba H.","Baba T.","Fujita K.","Hayashi K.","Inada T.","Isono K.","Itoh T.","Kasai H.","Kashimoto K.","Kimura S.","Kitakawa M.","Kitagawa M.","Makino K.","Miki T.","Mizobuchi K.","Mori H.","Mori T.","Motomura K.","Nakade S.","Nakamura Y.","Nashimoto H.","Nishio Y.","Oshima T.","Saito N.","Sampei G.","Seki Y.","Sivasundaram S.","Tagami H.","Takeda J.","Takemoto K.","Takeuchi Y.","Wada C.","Yamamoto Y.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9097039"},{"database":"DOI","id":"10.1093/dnares/3.6.363"}],"title":"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.","publicationDate":"1996","journal":"DNA Res.","firstPage":"363","lastPage":"377","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. 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