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Positively regulates proBA promoter activity specifically under glucose-inducing conditions and also post-transcriptionally up-regulates proBA mRNA levels (PubMed:39932325). Also acts as an RNA-binding protein which interacts with the RNA subunit of the ribonuclease P (RNase P) complex and negatively regulates the pre-tRNA processing activity of the complex (PubMed:38050972)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38050972"}],"value":"Interacts with the RNA subunit of the ribonuclease P (RNase P) complex; binds with very high affinity close to the region of the RNA subunit that is required for binding of pre-tRNAs; the interaction results in reduced RNase P complex activity and is not mediated by RNase P complex member RnpA"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30355672"}],"value":"Cytoplasm, nucleoid","id":"SL-0187"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30355672"}],"value":"Induced by glucose"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the YlxR/RnpM family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"Nucleoid-associated protein YlxR","featureId":"PRO_0000049629"},{"type":"Domain","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":82,"modifier":"EXACT"}},"description":"YlxR","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Mutagenesis","location":{"start":{"value":11,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"Severely reduces binding to the RNA subunit of the RNase P complex but residual is binding retained; when associated with W-15.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38050972"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["Y"]}},{"type":"Mutagenesis","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":13,"modifier":"EXACT"}},"description":"Abolishes binding to the RNA subunit of the RNase P complex; when associated with W-53.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38050972"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["Y"]}},{"type":"Mutagenesis","location":{"start":{"value":15,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"Severely reduces binding to the RNA subunit of the RNase P complex but residual is binding retained; when associated with Y-11.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38050972"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["W"]}},{"type":"Mutagenesis","location":{"start":{"value":53,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"Abolishes binding to the RNA subunit of the RNase P complex; when associated with Y-13.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38050972"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["W"]}}],"keywords":[{"id":"KW-0010","category":"Molecular function","name":"Activator"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0678","category":"Molecular function","name":"Repressor"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"}],"references":[{"referenceNumber":1,"citation":{"id":"8491709","citationType":"journal article","authors":["Shazand K.","Tucker J.","Grunberg-Manago M.","Rabinowitz J.C.","Leighton T."],"citationCrossReferences":[{"database":"PubMed","id":"8491709"},{"database":"DOI","id":"10.1128/jb.175.10.2880-2887.1993"}],"title":"Similar organization of the nusA-infB operon in Bacillus subtilis and Escherichia coli.","publicationDate":"1993","journal":"J. 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