{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P32701","secondaryAccessions":["P76789","Q2M6P3"],"uniProtkbId":"PDEC_ECOLI","entryAudit":{"firstPublicDate":"1993-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-11-01","entryVersion":153,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Probable cyclic di-GMP phosphodiesterase PdeC"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P21514"}],"value":"3.1.4.52"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26148715"}],"value":"pdeC"},"synonyms":[{"value":"yjcC"}],"orderedLocusNames":[{"value":"b4061"},{"value":"JW4022"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P21514"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19460094"}],"value":"Phosphodiesterase (PDE) that catalyzes the hydrolysis of cyclic-di-GMP (c-di-GMP) to 5'-pGpG (By similarity). Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria. Overexpression reduces biofilm formation (PubMed:19460094)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3',3'-c-di-GMP + H2O = 5'-phosphoguanylyl(3'->5')guanosine + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24902"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:58754"},{"database":"ChEBI","id":"CHEBI:58805"}],"ecNumber":"3.1.4.52","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P21514"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19332833"}],"value":"Expressed during transition into stationary phase, at both 28 and 37 degrees Celsius. Expression is RpoS dependent"}],"commentType":"INDUCTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":528,"modifier":"EXACT"}},"description":"Probable cyclic di-GMP phosphodiesterase PdeC","featureId":"PRO_0000169719"},{"type":"Transmembrane","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":242,"modifier":"EXACT"},"end":{"value":262,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Domain","location":{"start":{"value":268,"modifier":"EXACT"},"end":{"value":520,"modifier":"EXACT"}},"description":"EAL","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00074"}]}],"keywords":[{"id":"KW-0973","category":"Ligand","name":"c-di-GMP"},{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"8265357","citationType":"journal article","authors":["Blattner F.R.","Burland V.D.","Plunkett G. III","Sofia H.J.","Daniels D.L."],"citationCrossReferences":[{"database":"PubMed","id":"8265357"},{"database":"DOI","id":"10.1093/nar/21.23.5408"}],"title":"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.","publicationDate":"1993","journal":"Nucleic Acids Res.","firstPage":"5408","lastPage":"5417","volume":"21"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"19332833","citationType":"journal article","authors":["Sommerfeldt N.","Possling A.","Becker G.","Pesavento C.","Tschowri N.","Hengge R."],"citationCrossReferences":[{"database":"PubMed","id":"19332833"},{"database":"DOI","id":"10.1099/mic.0.024257-0"}],"title":"Gene expression patterns and differential input into curli fimbriae regulation of all GGDEF/EAL domain proteins in Escherichia coli.","publicationDate":"2009","journal":"Microbiology","firstPage":"1318","lastPage":"1331","volume":"155"},"referencePositions":["INDUCTION","RPOS-DEPENDENCE"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"19460094","citationType":"journal article","authors":["Boehm A.","Steiner S.","Zaehringer F.","Casanova A.","Hamburger F.","Ritz D.","Keck W.","Ackermann M.","Schirmer T.","Jenal U."],"citationCrossReferences":[{"database":"PubMed","id":"19460094"},{"database":"DOI","id":"10.1111/j.1365-2958.2009.06739.x"}],"title":"Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress.","publicationDate":"2009","journal":"Mol. Microbiol.","firstPage":"1500","lastPage":"1516","volume":"72"},"referencePositions":["FUNCTION IN BIOFILM FORMATION"],"referenceComments":[{"value":"K12 / AB400","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"26148715","citationType":"journal article","authors":["Hengge R.","Galperin M.Y.","Ghigo J.M.","Gomelsky M.","Green J.","Hughes K.T.","Jenal U.","Landini P."],"citationCrossReferences":[{"database":"PubMed","id":"26148715"},{"database":"DOI","id":"10.1128/jb.00424-15"}],"title":"Systematic nomenclature for GGDEF and EAL domain-containing cyclic di-GMP turnover proteins of Escherichia coli.","publicationDate":"2015","journal":"J. Bacteriol.","firstPage":"7","lastPage":"11","volume":"198"},"referencePositions":["NOMENCLATURE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U00006","properties":[{"key":"ProteinId","value":"AAC43155.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC77031.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE78063.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"D65214","properties":[{"key":"EntryName","value":"D65214"}]},{"database":"RefSeq","id":"NP_418485.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000019548.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P32701","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P32701","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4260840","properties":[{"key":"Interactions","value":"9"}]},{"database":"DIP","id":"DIP-12548N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P32701","properties":[{"key":"Number of interactors","value":"60"}]},{"database":"IntAct","id":"P32701","properties":[{"key":"Interactions","value":"6"}]},{"database":"STRING","id":"511145.b4061","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b4061","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC77031","properties":[{"key":"ProteinId","value":"AAC77031"},{"key":"GeneId","value":"b4061"}]},{"database":"GeneID","id":"948568","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW4022","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b4061","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_21945","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|511145.12.peg.4182","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB1882","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG4943","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_000445_131_1_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P32701","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"RLIAHFY","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9812358at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P32701","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:EG11938-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:EG11938-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"3.1.4.52","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"PRO","id":"PR:P32701","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15919996"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"29514851"}]},{"database":"GO","id":"GO:0071111","properties":[{"key":"GoTerm","value":"F:cyclic-guanylate-specific phosphodiesterase activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"29514851"}]},{"database":"GO","id":"GO:1900190","properties":[{"key":"GoTerm","value":"P:regulation of single-species biofilm formation"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd01948","properties":[{"key":"EntryName","value":"EAL"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.450:FF:000010","properties":[{"key":"EntryName","value":"Cyclic diguanylate phosphodiesterase (EAL) domain protein"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.450","properties":[{"key":"EntryName","value":"EAL domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR024744","properties":[{"key":"EntryName","value":"CSS-motif_dom"}]},{"database":"InterPro","id":"IPR001633","properties":[{"key":"EntryName","value":"EAL_dom"}]},{"database":"InterPro","id":"IPR035919","properties":[{"key":"EntryName","value":"EAL_sf"}]},{"database":"PANTHER","id":"PTHR33121:SF60","properties":[{"key":"EntryName","value":"CYCLIC DI-GMP PHOSPHODIESTERASE PDEC-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33121","properties":[{"key":"EntryName","value":"CYCLIC DI-GMP PHOSPHODIESTERASE PDEF"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF12792","properties":[{"key":"EntryName","value":"CSS-motif"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00563","properties":[{"key":"EntryName","value":"EAL"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00052","properties":[{"key":"EntryName","value":"EAL"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF141868","properties":[{"key":"EntryName","value":"EAL domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50883","properties":[{"key":"EntryName","value":"EAL"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRTFAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDNHFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVYSTKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPARELTRGQ","length":528,"molWeight":60801,"crc64":"F6E4819954912F31","md5":"1E90AF2A760A07849FB213A40F495859"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1},"countByFeatureType":{"Chain":1,"Transmembrane":2,"Domain":1},"uniParcId":"UPI000013B531"}}