{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P31847","secondaryAccessions":["P37951"],"uniProtkbId":"YPUA_BACSU","entryAudit":{"firstPublicDate":"1993-07-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1994-02-01","entryVersion":114,"sequenceVersion":2},"annotationScore":2.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Uncharacterized protein YpuA"}},"alternativeNames":[{"fullName":{"value":"ORFX19"}}]},"genes":[{"geneName":{"value":"ypuA"},"orderedLocusNames":[{"value":"BSU23370"}]}],"comments":[{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22882210"}],"value":"Present in detergent-resistant membrane (DRM) fractions that may be equivalent to eukaryotic membrane rafts; these rafts include proteins involved in signaling, molecule trafficking and protein secretion"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22882210"}],"value":"Cell membrane","id":"SL-0039"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22882210"}],"value":"Membrane raft","id":"SL-0370"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17434969"}],"value":"Transcribed under partial control of SigM ECF sigma factor (PubMed:17434969)"}],"commentType":"INDUCTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"Uncharacterized protein YpuA","featureId":"PRO_0000049726"}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"7934829","citationType":"journal article","authors":["Sorokin A.V.","Zumstein E.","Azevedo V.","Ehrlich S.D.","Serror P."],"citationCrossReferences":[{"database":"PubMed","id":"7934829"},{"database":"DOI","id":"10.1111/j.1365-2958.1993.tb02670.x"}],"title":"The organization of the Bacillus subtilis 168 chromosome region between the spoVA and serA genetic loci, based on sequence data.","publicationDate":"1993","journal":"Mol. Microbiol.","firstPage":"385","lastPage":"395","volume":"10"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / NRRL NRS-744 / VKM B-501","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9384377","citationType":"journal article","authors":["Kunst F.","Ogasawara N.","Moszer I.","Albertini A.M.","Alloni G.","Azevedo V.","Bertero M.G.","Bessieres P.","Bolotin A.","Borchert S.","Borriss R.","Boursier L.","Brans A.","Braun M.","Brignell S.C.","Bron S.","Brouillet S.","Bruschi C.V.","Caldwell B.","Capuano V.","Carter N.M.","Choi S.-K.","Codani J.-J.","Connerton I.F.","Cummings N.J.","Daniel R.A.","Denizot F.","Devine K.M.","Duesterhoeft A.","Ehrlich S.D.","Emmerson P.T.","Entian K.-D.","Errington J.","Fabret C.","Ferrari E.","Foulger D.","Fritz C.","Fujita M.","Fujita Y.","Fuma S.","Galizzi A.","Galleron N.","Ghim S.-Y.","Glaser P.","Goffeau A.","Golightly E.J.","Grandi G.","Guiseppi G.","Guy B.J.","Haga K.","Haiech J.","Harwood C.R.","Henaut A.","Hilbert H.","Holsappel S.","Hosono S.","Hullo M.-F.","Itaya M.","Jones L.-M.","Joris B.","Karamata D.","Kasahara Y.","Klaerr-Blanchard M.","Klein C.","Kobayashi Y.","Koetter P.","Koningstein G.","Krogh S.","Kumano M.","Kurita K.","Lapidus A.","Lardinois S.","Lauber J.","Lazarevic V.","Lee S.-M.","Levine A.","Liu H.","Masuda S.","Mauel C.","Medigue C.","Medina N.","Mellado R.P.","Mizuno M.","Moestl D.","Nakai S.","Noback M.","Noone D.","O'Reilly M.","Ogawa K.","Ogiwara A.","Oudega B.","Park S.-H.","Parro V.","Pohl T.M.","Portetelle D.","Porwollik S.","Prescott A.M.","Presecan E.","Pujic P.","Purnelle B.","Rapoport G.","Rey M.","Reynolds S.","Rieger M.","Rivolta C.","Rocha E.","Roche B.","Rose M.","Sadaie Y.","Sato T.","Scanlan E.","Schleich S.","Schroeter R.","Scoffone F.","Sekiguchi J.","Sekowska A.","Seror S.J.","Serror P.","Shin B.-S.","Soldo B.","Sorokin A.","Tacconi E.","Takagi T.","Takahashi H.","Takemaru K.","Takeuchi M.","Tamakoshi A.","Tanaka T.","Terpstra P.","Tognoni A.","Tosato V.","Uchiyama S.","Vandenbol M.","Vannier F.","Vassarotti A.","Viari A.","Wambutt R.","Wedler E.","Wedler H.","Weitzenegger T.","Winters P.","Wipat A.","Yamamoto H.","Yamane K.","Yasumoto K.","Yata K.","Yoshida K.","Yoshikawa H.-F.","Zumstein E.","Yoshikawa H.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"9384377"},{"database":"DOI","id":"10.1038/36786"}],"title":"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.","publicationDate":"1997","journal":"Nature","firstPage":"249","lastPage":"256","volume":"390"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"1368705","citationType":"journal article","authors":["Yamamoto J.","Shimizu M.","Yamane K."],"citationCrossReferences":[{"database":"PubMed","id":"1368705"},{"database":"DOI","id":"10.1271/bbb1961.55.1615"}],"title":"Molecular cloning and analysis of nucleotide sequence of the Bacillus subtilis lysA gene region using B. subtilis phage vectors and a multi-copy plasmid, pUB110.","publicationDate":"1991","journal":"Agric. Biol. Chem.","firstPage":"1615","lastPage":"1626","volume":"55"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 113-290"]},{"referenceNumber":4,"citation":{"id":"8022278","citationType":"journal article","authors":["Herrler M.","Bang H.","Marahiel M.A."],"citationCrossReferences":[{"database":"PubMed","id":"8022278"},{"database":"DOI","id":"10.1111/j.1365-2958.1994.tb00384.x"}],"title":"Cloning and characterization of ppiB, a Bacillus subtilis gene which encodes a cyclosporin A-sensitive peptidyl-prolyl cis-trans isomerase.","publicationDate":"1994","journal":"Mol. Microbiol.","firstPage":"1073","lastPage":"1083","volume":"11"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 113-290"],"referenceComments":[{"value":"168 / JH642","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"17434969","citationType":"journal article","authors":["Jervis A.J.","Thackray P.D.","Houston C.W.","Horsburgh M.J.","Moir A."],"citationCrossReferences":[{"database":"PubMed","id":"17434969"},{"database":"DOI","id":"10.1128/jb.00130-07"}],"title":"SigM-responsive genes of Bacillus subtilis and their promoters.","publicationDate":"2007","journal":"J. Bacteriol.","firstPage":"4534","lastPage":"4538","volume":"189"},"referencePositions":["INDUCTION"],"referenceComments":[{"value":"168 / 1604","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"22882210","citationType":"journal article","authors":["Yepes A.","Schneider J.","Mielich B.","Koch G.","Garcia-Betancur J.C.","Ramamurthi K.S.","Vlamakis H.","Lopez D."],"citationCrossReferences":[{"database":"PubMed","id":"22882210"},{"database":"DOI","id":"10.1111/j.1365-2958.2012.08205.x"}],"title":"The biofilm formation defect of a Bacillus subtilis flotillin-defective mutant involves the protease FtsH.","publicationDate":"2012","journal":"Mol. Microbiol.","firstPage":"457","lastPage":"471","volume":"86"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY","SUBCELLULAR LOCATION"],"referenceComments":[{"value":"168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / NRRL NRS-744 / VKM B-501","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L09228","properties":[{"key":"ProteinId","value":"AAA67474.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL009126","properties":[{"key":"ProteinId","value":"CAB14269.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"D90189","properties":[{"key":"ProteinId","value":"BAA14212.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"X73898","properties":[{"key":"ProteinId","value":"CAA52102.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S45536","properties":[{"key":"EntryName","value":"JU0473"}]},{"database":"RefSeq","id":"NP_390218.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"RefSeq","id":"WP_009967658.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"AlphaFoldDB","id":"P31847","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P31847","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P31847","properties":[{"key":"Number of interactors","value":"25"}]},{"database":"STRING","id":"224308.BSU23370","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"224308-BSU23370","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"938939","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAB14269","properties":[{"key":"ProteinId","value":"CAB14269"},{"key":"GeneId","value":"BSU_23370"}]},{"database":"GeneID","id":"938939","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bsu:BSU23370","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|224308.179.peg.2546","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG4086","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"P31847","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9810153at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P31847","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"BSUB:BSU23370-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001570","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P31847","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0045121","properties":[{"key":"GoTerm","value":"C:membrane raft"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22882210"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22882210"}]},{"database":"InterPro","id":"IPR009343","properties":[{"key":"EntryName","value":"DUF1002"}]},{"database":"Pfam","id":"PF06207","properties":[{"key":"EntryName","value":"DUF1002"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKKIWIGMLAAAVLLLMVPKVSLADAAVGDVIVTLGADLSESDKQKVLDEMNVPDNATTVTVTNKEEHEYLGKYISNAQIGSRAISSSSITIAKKGSGLNVETHNISGITDEMYLNALMTAGVKDAKVYVTAPFEVSGTAALTGLIKAYEVSSDEAISEDVKQVANQELVTTSELGDKIGNENAAALIAKIKEEFAKNGVPDNKADIEKQVDDAASDLNVTLTDSQKNQLVSLFNKMKNADIDWGQVSDQLDKAKDKITKFIESDEGKNFIQKVIDFFVSIWNAIVSIFK","length":290,"molWeight":31295,"crc64":"06F145CBB96DCDC3","md5":"CC01CC88287AC314E496A65DAE9F2194"},"extraAttributes":{"countByCommentType":{"SUBCELLULAR LOCATION":1,"INDUCTION":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI000006068F"}}