{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P31677","uniProtkbId":"OTSA_ECOLI","entryAudit":{"firstPublicDate":"1993-07-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-01-23","entryVersion":181,"sequenceVersion":3},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"3131312"}],"value":"Trehalose-6-phosphate synthase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"3131312"}],"value":"TPS"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"20077550"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"3131312"}],"value":"2.4.1.15"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Alpha,alpha-trehalose-phosphate synthase [UDP-forming]"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"3131312"}],"value":"Osmoregulatory trehalose synthesis protein A"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"3131312"}],"value":"OtsA"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"UDP-glucose-glucosephosphate glucosyltransferase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"3131312"}],"value":"otsA"},"orderedLocusNames":[{"value":"b1896"},{"value":"JW5312"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12105274"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1310094"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20077550"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"3131312"}],"value":"Catalyzes the transfer of glucose from UDP-alpha-D-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor. Essential for viability of the cells at low temperatures and at elevated osmotic strength"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-trehalose 6-phosphate + UDP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:18889"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:58223"},{"database":"ChEBI","id":"CHEBI:58429"},{"database":"ChEBI","id":"CHEBI:58885"},{"database":"ChEBI","id":"CHEBI:61548"}],"ecNumber":"2.4.1.15","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"20077550"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"3131312"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20077550"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"3131312"}],"value":"Activated by potassium and other monovalent cations at 0.25 M, but partially inhibited at greater concentrations (PubMed:3131312). Inhibited by validoxylamine A 6'-O-phosphate (PubMed:20077550)"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":1.7,"unit":"mM","substrate":"UDP-Glc","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20077550"}]},{"constant":7.3,"unit":"mM","substrate":"Glc-6-P","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20077550"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20077550"}],"value":"kcat is 34 sec(-1) for glucosyltransferase activity."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"3131312"}],"value":"Glycan biosynthesis; trehalose biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12498887"}],"value":"Homotetramer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12105274"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1310094"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1744047"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"3131312"}],"value":"By cold-shock, osmotic-shock and during the transition to stationary phase. Expression is partially dependent on RpoS"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"3131312"}],"value":"Mutants are viable, but osmotically sensitive in minimal media and sensitive to cold shock"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyltransferase 20 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed"},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":474,"modifier":"EXACT"}},"description":"Trehalose-6-phosphate synthase","featureId":"PRO_0000122489"},{"type":"Binding site","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":10,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:61548"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12498887"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20077550"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1GZ5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2WTX"}],"ligand":{"name":"D-glucose 6-phosphate","id":"ChEBI:CHEBI:61548"}},{"type":"Binding site","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58885"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12498887"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"14570926"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"20077550"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1GZ5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2WTX"}],"ligand":{"name":"UDP-alpha-D-glucose","id":"ChEBI:CHEBI:58885"}},{"type":"Binding site","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:61548"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12498887"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20077550"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1GZ5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2WTX"}],"ligand":{"name":"D-glucose 6-phosphate","id":"ChEBI:CHEBI:61548"}},{"type":"Binding site","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:61548"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12498887"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20077550"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1GZ5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2WTX"}],"ligand":{"name":"D-glucose 6-phosphate","id":"ChEBI:CHEBI:61548"}},{"type":"Binding site","location":{"start":{"value":263,"modifier":"EXACT"},"end":{"value":263,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58885"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12498887"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"14570926"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"20077550"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1GZ5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1UQT"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1UQU"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2WTX"}],"ligand":{"name":"UDP-alpha-D-glucose","id":"ChEBI:CHEBI:58885"}},{"type":"Binding site","location":{"start":{"value":268,"modifier":"EXACT"},"end":{"value":268,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58885"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12498887"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"14570926"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"20077550"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1GZ5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1UQT"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1UQU"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2WTX"}],"ligand":{"name":"UDP-alpha-D-glucose","id":"ChEBI:CHEBI:58885"}},{"type":"Binding site","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:61548"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12498887"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20077550"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1GZ5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2WTX"}],"ligand":{"name":"D-glucose 6-phosphate","id":"ChEBI:CHEBI:61548"}},{"type":"Binding site","location":{"start":{"value":340,"modifier":"EXACT"},"end":{"value":340,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58885"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12498887"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"14570926"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"20077550"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1GZ5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1UQT"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1UQU"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2WTX"}],"ligand":{"name":"UDP-alpha-D-glucose","id":"ChEBI:CHEBI:58885"}},{"type":"Binding site","location":{"start":{"value":366,"modifier":"EXACT"},"end":{"value":370,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58885"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12498887"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"14570926"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"20077550"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1GZ5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1UQT"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1UQU"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2WTX"}],"ligand":{"name":"UDP-alpha-D-glucose","id":"ChEBI:CHEBI:58885"}},{"type":"Site","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":86,"modifier":"EXACT"}},"description":"Involved in alpha anomer selectivity","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12498887"}]},{"type":"Site","location":{"start":{"value":156,"modifier":"EXACT"},"end":{"value":156,"modifier":"EXACT"}},"description":"Involved in alpha anomer selectivity","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12498887"}]},{"type":"Beta strand","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":10,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":18,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1GZ5"}]},{"type":"Helix","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":47,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":93,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":100,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":118,"modifier":"EXACT"},"end":{"value":120,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":137,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":153,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":157,"modifier":"EXACT"},"end":{"value":160,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2WTX"}]},{"type":"Helix","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":170,"modifier":"EXACT"},"end":{"value":177,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":180,"modifier":"EXACT"},"end":{"value":187,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":188,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":204,"modifier":"EXACT"},"end":{"value":207,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Turn","location":{"start":{"value":208,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":214,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":217,"modifier":"EXACT"},"end":{"value":223,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":230,"modifier":"EXACT"},"end":{"value":238,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":240,"modifier":"EXACT"},"end":{"value":242,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":243,"modifier":"EXACT"},"end":{"value":251,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Turn","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":254,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":261,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":266,"modifier":"EXACT"},"end":{"value":268,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":270,"modifier":"EXACT"},"end":{"value":283,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":285,"modifier":"EXACT"},"end":{"value":287,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Turn","location":{"start":{"value":288,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":291,"modifier":"EXACT"},"end":{"value":296,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":298,"modifier":"EXACT"},"end":{"value":300,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6JAK"}]},{"type":"Helix","location":{"start":{"value":305,"modifier":"EXACT"},"end":{"value":325,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":332,"modifier":"EXACT"},"end":{"value":336,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":342,"modifier":"EXACT"},"end":{"value":351,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":353,"modifier":"EXACT"},"end":{"value":357,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":360,"modifier":"EXACT"},"end":{"value":363,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":366,"modifier":"EXACT"},"end":{"value":374,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":382,"modifier":"EXACT"},"end":{"value":386,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":390,"modifier":"EXACT"},"end":{"value":393,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Beta strand","location":{"start":{"value":398,"modifier":"EXACT"},"end":{"value":401,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":406,"modifier":"EXACT"},"end":{"value":417,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":421,"modifier":"EXACT"},"end":{"value":437,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]},{"type":"Helix","location":{"start":{"value":440,"modifier":"EXACT"},"end":{"value":453,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UQT"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0328","category":"Molecular function","name":"Glycosyltransferase"},{"id":"KW-0630","category":"Ligand","name":"Potassium"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0346","category":"Biological process","name":"Stress response"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"8045430","citationType":"journal article","authors":["Kaasen I.","McDougall J.","Stroem A.R."],"citationCrossReferences":[{"database":"PubMed","id":"8045430"},{"database":"DOI","id":"10.1016/0378-1119(94)90316-6"}],"title":"Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex.","publicationDate":"1994","journal":"Gene","firstPage":"9","lastPage":"15","volume":"145"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","PARTIAL PROTEIN SEQUENCE"],"referenceComments":[{"value":"K12 / CSH7","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9097040","citationType":"journal article","authors":["Itoh T.","Aiba H.","Baba T.","Fujita K.","Hayashi K.","Inada T.","Isono K.","Kasai H.","Kimura S.","Kitakawa M.","Kitagawa M.","Makino K.","Miki T.","Mizobuchi K.","Mori H.","Mori T.","Motomura K.","Nakade S.","Nakamura Y.","Nashimoto H.","Nishio Y.","Oshima T.","Saito N.","Sampei G.","Seki Y.","Sivasundaram S.","Tagami H.","Takeda J.","Takemoto K.","Wada C.","Yamamoto Y.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9097040"},{"database":"DOI","id":"10.1093/dnares/3.6.379"}],"title":"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.","publicationDate":"1996","journal":"DNA Res.","firstPage":"379","lastPage":"392","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-6QKQP44VCDA36","citationType":"submission","authors":["Estep P.","O'Keeffe T.","Robison K.","Church G.M."],"publicationDate":"MAY-1995","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 430-474"],"referenceComments":[{"value":"K12 / EMG2","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"3131312","citationType":"journal article","authors":["Giaever H.M.","Styrvold O.B.","Kaasen I.","Stroem A.R."],"citationCrossReferences":[{"database":"PubMed","id":"3131312"},{"database":"DOI","id":"10.1128/jb.170.6.2841-2849.1988"}],"title":"Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli.","publicationDate":"1988","journal":"J. Bacteriol.","firstPage":"2841","lastPage":"2849","volume":"170"},"referencePositions":["FUNCTION AS A TREHALOSE-6-PHOSPHATE SYNTHASE","CATALYTIC ACTIVITY","ACTIVITY REGULATION","DISRUPTION PHENOTYPE","INDUCTION","NOMENCLATURE","PATHWAY"]},{"referenceNumber":7,"citation":{"id":"1744047","citationType":"journal article","authors":["Hengge-Aronis R.","Klein W.","Lange R.","Rimmele M.","Boos W."],"citationCrossReferences":[{"database":"PubMed","id":"1744047"},{"database":"DOI","id":"10.1128/jb.173.24.7918-7924.1991"}],"title":"Trehalose synthesis genes are controlled by the putative sigma factor encoded by rpoS and are involved in stationary-phase thermotolerance in Escherichia coli.","publicationDate":"1991","journal":"J. Bacteriol.","firstPage":"7918","lastPage":"7924","volume":"173"},"referencePositions":["INDUCTION"]},{"referenceNumber":8,"citation":{"id":"1310094","citationType":"journal article","authors":["Kaasen I.","Falkenberg P.","Styrvold O.B.","Strom A.R."],"citationCrossReferences":[{"database":"PubMed","id":"1310094"},{"database":"DOI","id":"10.1128/jb.174.3.889-898.1992"}],"title":"Molecular cloning and physical mapping of the otsBA genes, which encode the osmoregulatory trehalose pathway of Escherichia coli: evidence that transcription is activated by katF (AppR).","publicationDate":"1992","journal":"J. Bacteriol.","firstPage":"889","lastPage":"898","volume":"174"},"referencePositions":["FUNCTION AS A TREHALOSE-6-PHOSPHATE SYNTHASE","INDUCTION"]},{"referenceNumber":9,"citation":{"id":"12105274","citationType":"journal article","authors":["Kandror O.","DeLeon A.","Goldberg A.L."],"citationCrossReferences":[{"database":"PubMed","id":"12105274"},{"database":"DOI","id":"10.1073/pnas.142314099"}],"title":"Trehalose synthesis is induced upon exposure of Escherichia coli to cold and is essential for viability at low temperatures.","publicationDate":"2002","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"9727","lastPage":"9732","volume":"99"},"referencePositions":["FUNCTION AS A TREHALOSE-6-PHOSPHATE SYNTHASE","INDUCTION"],"referenceComments":[{"value":"K12 / MC4100 / ATCC 35695 / DSM 6574","type":"STRAIN"}]},{"referenceNumber":10,"citation":{"id":"12498887","citationType":"journal article","authors":["Gibson R.P.","Turkenburg J.P.","Charnock S.J.","Lloyd R.","Davies G.J."],"citationCrossReferences":[{"database":"PubMed","id":"12498887"},{"database":"DOI","id":"10.1016/s1074-5521(02)00292-2"}],"title":"Insights into trehalose synthesis provided by the structure of the retaining glucosyltransferase OtsA.","publicationDate":"2002","journal":"Chem. Biol.","firstPage":"1337","lastPage":"1346","volume":"9"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.43 ANGSTROMS) OF 1-457 IN COMPLEX WITH SUBSTRATE ANALOG AND GLUCOSE-6-PHOSPHATE","SUBUNIT"]},{"referenceNumber":11,"citation":{"id":"14570926","citationType":"journal article","authors":["Gibson R.P.","Tarling C.A.","Roberts S.","Withers S.G.","Davies G.J."],"citationCrossReferences":[{"database":"PubMed","id":"14570926"},{"database":"DOI","id":"10.1074/jbc.m307643200"}],"title":"The donor subsite of trehalose-6-phosphate synthase: binary complexes with UDP-glucose and UDP-2-deoxy-2-fluoro-glucose at 2 A resolution.","publicationDate":"2004","journal":"J. Biol. Chem.","firstPage":"1950","lastPage":"1955","volume":"279"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG"]},{"referenceNumber":12,"citation":{"id":"20077550","citationType":"journal article","authors":["Errey J.C.","Lee S.S.","Gibson R.P.","Martinez Fleites C.","Barry C.S.","Jung P.M.","O'Sullivan A.C.","Davis B.G.","Davies G.J."],"citationCrossReferences":[{"database":"PubMed","id":"20077550"},{"database":"DOI","id":"10.1002/anie.200905096"}],"title":"Mechanistic insight into enzymatic glycosyl transfer with retention of configuration through analysis of glycomimetic inhibitors.","publicationDate":"2010","journal":"Angew. Chem. Int. Ed. Engl.","firstPage":"1234","lastPage":"1237","volume":"49"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOGS","FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","ACTIVITY REGULATION","REACTION MECHANISM"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X69160","properties":[{"key":"ProteinId","value":"CAA48913.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC74966.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA15717.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U27211","properties":[{"key":"ProteinId","value":"AAA68604.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"I83402","properties":[{"key":"EntryName","value":"I83402"}]},{"database":"RefSeq","id":"NP_416410.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001295646.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"PDB","id":"1GZ5","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.43 A"},{"key":"Chains","value":"A/B/C/D=2-457"}]},{"database":"PDB","id":"1UQT","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.00 A"},{"key":"Chains","value":"A/B=1-474"}]},{"database":"PDB","id":"1UQU","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.00 A"},{"key":"Chains","value":"A/B=1-474"}]},{"database":"PDB","id":"2WTX","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"A/B/C/D=1-474"}]},{"database":"PDB","id":"6JAK","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.41 A"},{"key":"Chains","value":"A/B/C/D=2-456"}]},{"database":"PDBsum","id":"1GZ5","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1UQT","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1UQU","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2WTX","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"6JAK","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P31677","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P31677","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4259473","properties":[{"key":"Interactions","value":"17"}]},{"database":"DIP","id":"DIP-10417N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P31677","properties":[{"key":"Number of interactors","value":"271"}]},{"database":"IntAct","id":"P31677","properties":[{"key":"Interactions","value":"10"}]},{"database":"STRING","id":"511145.b1896","properties":[{"key":"Description","value":"-"}]},{"database":"DrugBank","id":"DB02007","properties":[{"key":"GenericName","value":"alpha-D-glucose 6-phosphate"}]},{"database":"DrugBank","id":"DB03366","properties":[{"key":"GenericName","value":"Imidazole"}]},{"database":"DrugBank","id":"DB01861","properties":[{"key":"GenericName","value":"Uridine diphosphate glucose"}]},{"database":"DrugBank","id":"DB03435","properties":[{"key":"GenericName","value":"Uridine-5'-Diphosphate"}]},{"database":"DrugBank","id":"DB03488","properties":[{"key":"GenericName","value":"Uridine-5'-diphosphate-2-deoxy-2-fluoro-alpha-D-galactose"}]},{"database":"CAZy","id":"GT20","properties":[{"key":"FamilyName","value":"Glycosyltransferase Family 20"}]},{"database":"jPOST","id":"P31677","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b1896","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC74966","properties":[{"key":"ProteinId","value":"AAC74966"},{"key":"GeneId","value":"b1896"}]},{"database":"GeneID","id":"93776199","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"946405","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW5312","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b1896","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_10770","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.353","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB1701","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0380","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_002351_7_1_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P31677","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"NRTIWPL","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9815690at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P31677","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:TREHALOSE6PSYN-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:TREHALOSE6PSYN-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"2.4.1.15","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"BRENDA","id":"3.1.3.12","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"UniPathway","id":"UPA00299","properties":[{"key":"RectionId","value":"-"}]},{"database":"EvolutionaryTrace","id":"P31677","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P31677","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P31677","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0003825","properties":[{"key":"GoTerm","value":"F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"11807274"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"3131312"}]},{"database":"GO","id":"GO:0070417","properties":[{"key":"GoTerm","value":"P:cellular response to cold"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"12105274"}]},{"database":"GO","id":"GO:0006974","properties":[{"key":"GoTerm","value":"P:DNA damage response"},{"key":"GoEvidenceType","value":"IEP:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000270","source":"PubMed","id":"11967071"}]},{"database":"GO","id":"GO:0006970","properties":[{"key":"GoTerm","value":"P:response to osmotic stress"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"3131312"}]},{"database":"GO","id":"GO:0006950","properties":[{"key":"GoTerm","value":"P:response to stress"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"16000787"}]},{"database":"GO","id":"GO:0005992","properties":[{"key":"GoTerm","value":"P:trehalose biosynthetic process"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"3131312"},{"evidenceCode":"ECO:0000315","source":"PubMed","id":"8837441"}]},{"database":"CDD","id":"cd03788","properties":[{"key":"EntryName","value":"GT20_TPS"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.2000:FF:000024","properties":[{"key":"EntryName","value":"Trehalose-6-phosphate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.2000","properties":[{"key":"EntryName","value":"Glycogen Phosphorylase B"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR001830","properties":[{"key":"EntryName","value":"Glyco_trans_20"}]},{"database":"InterPro","id":"IPR012766","properties":[{"key":"EntryName","value":"Trehalose_OtsA"}]},{"database":"NCBIfam","id":"NF007513","properties":[{"key":"EntryName","value":"PRK10117.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR02400","properties":[{"key":"EntryName","value":"trehalose_OtsA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10788:SF106","properties":[{"key":"EntryName","value":"BCDNA.GH08860"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10788","properties":[{"key":"EntryName","value":"TREHALOSE-6-PHOSPHATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00982","properties":[{"key":"EntryName","value":"Glyco_transf_20"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53756","properties":[{"key":"EntryName","value":"UDP-Glycosyltransferase/glycogen phosphorylase"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSGETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPAWDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPTPEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRSAESQQRDKVATFPKLA","length":474,"molWeight":53611,"crc64":"63FFB6F938056F95","md5":"1446729BEC3DA4AE5A0B090583DEF8A9"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"ACTIVITY REGULATION":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBUNIT":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Initiator methionine":1,"Chain":1,"Binding site":9,"Site":2,"Beta strand":17,"Helix":27,"Turn":3},"uniParcId":"UPI00001680E7"}}