{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P31660","secondaryAccessions":["P77217","Q2MC91"],"uniProtkbId":"PRPC_ECOLI","entryAudit":{"firstPublicDate":"1993-07-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-11-01","entryVersion":172,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9579066"}],"value":"2-methylcitrate synthase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9579066"}],"value":"2-MCS"},{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9579066"}],"value":"MCS"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12473114"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9325432"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9579066"}],"value":"2.3.3.5"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12473114"}],"value":"(2S,3S)-2-methylcitrate synthase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"8508809"}],"value":"Citrate synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9325432"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9579066"}],"value":"2.3.3.16"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9579066"}],"value":"prpC"},"synonyms":[{"value":"yahS"},{"value":"yzzD"}],"orderedLocusNames":[{"value":"b0333"},{"value":"JW0324"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12473114"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28956599"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8508809"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9325432"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9579066"}],"value":"Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the Claisen condensation of propionyl-CoA and oxaloacetate (OAA) to yield 2-methylcitrate (2-MC) and CoA. Also catalyzes the condensation of oxaloacetate with acetyl-CoA to yield citrate but with a lower specificity"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"propanoyl-CoA + oxaloacetate + H2O = (2S,3S)-2-methylcitrate + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:23780"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16452"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57392"},{"database":"ChEBI","id":"CHEBI:58853"}],"ecNumber":"2.3.3.5","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12473114"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9325432"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9579066"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16845"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16452"},{"database":"ChEBI","id":"CHEBI:16947"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"}],"ecNumber":"2.3.3.16","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9325432"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9579066"}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":0.11,"unit":"umol/min/mg","enzyme":"enzyme with acetyl-CoA as substrate (at pH 8 and 35 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9579066"}]},{"velocity":0.33,"unit":"umol/min/mg","enzyme":"enzyme with propionyl-CoA as substrate (at pH 8 and 35 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9579066"}]}],"michaelisConstants":[{"constant":5.0,"unit":"uM","substrate":"oxaloacetate (at pH 8 and 35 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9579066"}]},{"constant":17.0,"unit":"uM","substrate":"propionyl-CoA (at pH 7.4)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9325432"}]},{"constant":37.0,"unit":"uM","substrate":"propionyl-CoA (at pH 8 and 35 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9579066"}]},{"constant":101.0,"unit":"uM","substrate":"acetyl-CoA (at pH 8 and 35 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9579066"}]}]},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9325432"}],"value":"Optimum pH is 9."}]},"temperatureDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9325432"}],"value":"Optimum temperature is between 45 and 50 degrees Celsius."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9325432"}],"value":"Organic acid metabolism; propanoate degradation"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9325432"}],"value":"Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9579066"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12473114"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15805526"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22579471"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9325432"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9579066"}],"value":"By propionate, but not acetate or glucose. Expression of prpBCDE operon is regulated by PrpR, CRP and a variety of sugars such as arabinose, galactose, glucose mannose and xylose"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the citrate synthase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12473114"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"28956599"}],"value":"There is uncertainty concerning the 2-methylcitrate stereochemistry. Brock et al. report a (2S,3S) stereochemistry, but Reddick et al. determined that the 2-methylcitrate has either (2S,3R) or (2R,3S) stereochemistry"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":389,"modifier":"EXACT"}},"description":"2-methylcitrate synthase","featureId":"PRO_0000169981"},{"type":"Active site","location":{"start":{"value":235,"modifier":"EXACT"},"end":{"value":235,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O34002"}]},{"type":"Active site","location":{"start":{"value":274,"modifier":"EXACT"},"end":{"value":274,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O34002"}]},{"type":"Active site","location":{"start":{"value":325,"modifier":"EXACT"},"end":{"value":325,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O34002"}]},{"type":"Binding site","location":{"start":{"value":82,"modifier":"EXACT"},"end":{"value":82,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"I6Y9Q3"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":200,"modifier":"EXACT"},"end":{"value":200,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"I6Y9Q3"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":268,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57287"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O34002"}],"ligand":{"name":"CoA","id":"ChEBI:CHEBI:57287"}},{"type":"Binding site","location":{"start":{"value":283,"modifier":"EXACT"},"end":{"value":283,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"I6Y9Q3"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":350,"modifier":"EXACT"},"end":{"value":350,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"I6Y9Q3"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":369,"modifier":"EXACT"},"end":{"value":369,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"I6Y9Q3"}],"ligand":{"name":"substrate"}}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0816","category":"Biological process","name":"Tricarboxylic acid cycle"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-B1UJ6TNQ0SAS1","citationType":"submission","authors":["Chung E.","Allen E.","Araujo R.","Aparicio A.M.","Davis K.","Duncan M.","Federspiel N.","Hyman R.","Kalman S.","Komp C.","Kurdi O.","Lew H.","Lin D.","Namath A.","Oefner P.","Roberts D.","Schramm S.","Davis R.W."],"title":"Sequence of minutes 4-25 of Escherichia coli.","publicationDate":"JAN-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"8508809","citationType":"journal article","authors":["Patton A.J.","Hough D.W.","Towner P.","Danson M.J."],"citationCrossReferences":[{"database":"PubMed","id":"8508809"},{"database":"DOI","id":"10.1111/j.1432-1033.1993.tb17898.x"}],"title":"Does Escherichia coli possess a second citrate synthase gene?","publicationDate":"1993","journal":"Eur. J. Biochem.","firstPage":"75","lastPage":"81","volume":"214"},"referencePositions":["PROTEIN SEQUENCE OF 18-54","FUNCTION"]},{"referenceNumber":5,"citation":{"id":"9325432","citationType":"journal article","authors":["Textor S.","Wendisch V.F.","de Graaf A.A.","Mueller U.","Linder M.I.","Linder D.","Buckel W."],"citationCrossReferences":[{"database":"PubMed","id":"9325432"},{"database":"DOI","id":"10.1007/s002030050518"}],"title":"Propionate oxidation in Escherichia coli: evidence for operation of a methylcitrate cycle in bacteria.","publicationDate":"1997","journal":"Arch. Microbiol.","firstPage":"428","lastPage":"436","volume":"168"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","INDUCTION"]},{"referenceNumber":6,"citation":{"id":"9579066","citationType":"journal article","authors":["Gerike U.","Hough D.W.","Russell N.J.","Dyall-Smith M.L.","Danson M.J."],"citationCrossReferences":[{"database":"PubMed","id":"9579066"},{"database":"DOI","id":"10.1099/00221287-144-4-929"}],"title":"Citrate synthase and 2-methylcitrate synthase: structural, functional and evolutionary relationships.","publicationDate":"1998","journal":"Microbiology","firstPage":"929","lastPage":"935","volume":"144"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","INDUCTION","SUBSTRATE SPECIFICITY","SUBUNIT"]},{"referenceNumber":7,"citation":{"id":"12473114","citationType":"journal article","authors":["Brock M.","Maerker C.","Schuetz A.","Voelker U.","Buckel W."],"citationCrossReferences":[{"database":"PubMed","id":"12473114"},{"database":"DOI","id":"10.1046/j.1432-1033.2002.03336.x"}],"title":"Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase.","publicationDate":"2002","journal":"Eur. J. Biochem.","firstPage":"6184","lastPage":"6194","volume":"269"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","INDUCTION"]},{"referenceNumber":8,"citation":{"id":"15805526","citationType":"journal article","authors":["Lee S.K.","Newman J.D.","Keasling J.D."],"citationCrossReferences":[{"database":"PubMed","id":"15805526"},{"database":"DOI","id":"10.1128/jb.187.8.2793-2800.2005"}],"title":"Catabolite repression of the propionate catabolic genes in Escherichia coli and Salmonella enterica: evidence for involvement of the cyclic AMP receptor protein.","publicationDate":"2005","journal":"J. Bacteriol.","firstPage":"2793","lastPage":"2800","volume":"187"},"referencePositions":["INDUCTION"]},{"referenceNumber":9,"citation":{"id":"22579471","citationType":"journal article","authors":["Park J.M.","Vinuselvi P.","Lee S.K."],"citationCrossReferences":[{"database":"PubMed","id":"22579471"},{"database":"DOI","id":"10.1016/j.gene.2012.04.074"}],"title":"The mechanism of sugar-mediated catabolite repression of the propionate catabolic genes in Escherichia coli.","publicationDate":"2012","journal":"Gene","firstPage":"116","lastPage":"121","volume":"504"},"referencePositions":["INDUCTION"]},{"referenceNumber":10,"citation":{"id":"28956599","citationType":"journal article","authors":["Reddick J.J.","Sirkisoon S.","Dahal R.A.","Hardesty G.","Hage N.E.","Booth W.T.","Quattlebaum A.L.","Mills S.N.","Meadows V.G.","Adams S.L.H.","Doyle J.S.","Kiel B.E."],"citationCrossReferences":[{"database":"PubMed","id":"28956599"},{"database":"DOI","id":"10.1021/acs.biochem.7b00778"}],"title":"First biochemical characterization of a methylcitric acid cycle from Bacillus subtilis strain 168.","publicationDate":"2017","journal":"Biochemistry","firstPage":"5698","lastPage":"5711","volume":"56"},"referencePositions":["FUNCTION","DISCUSSION OF STEREOCHEMISTRY"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U73857","properties":[{"key":"ProteinId","value":"AAB18057.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC73436.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE76115.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"E64760","properties":[{"key":"EntryName","value":"E64760"}]},{"database":"RefSeq","id":"NP_414867.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001285927.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P31660","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P31660","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4259809","properties":[{"key":"Interactions","value":"10"}]},{"database":"DIP","id":"DIP-10579N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P31660","properties":[{"key":"Number of interactors","value":"527"}]},{"database":"IntAct","id":"P31660","properties":[{"key":"Interactions","value":"2"}]},{"database":"STRING","id":"511145.b0333","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P31660","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b0333","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC73436","properties":[{"key":"ProteinId","value":"AAC73436"},{"key":"GeneId","value":"b0333"}]},{"database":"GeneID","id":"947528","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW0324","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b0333","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_01630","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_24800","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.1944","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB1706","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0372","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_025068_2_1_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P31660","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"NEAAMDM","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9800864at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P31660","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:G6198-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:G6198-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"2.3.3.5","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"UniPathway","id":"UPA00223","properties":[{"key":"RectionId","value":"UER00717"}]},{"database":"UniPathway","id":"UPA00946","properties":[{"key":"RectionId","value":"-"}]},{"database":"PRO","id":"PR:P31660","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P31660","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0050440","properties":[{"key":"GoTerm","value":"F:2-methylcitrate synthase activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"9579066"}]},{"database":"GO","id":"GO:0036440","properties":[{"key":"GoTerm","value":"F:citrate synthase activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"9325432"}]},{"database":"GO","id":"GO:0042803","properties":[{"key":"GoTerm","value":"F:protein homodimerization activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"8508809"}]},{"database":"GO","id":"GO:0005975","properties":[{"key":"GoTerm","value":"P:carbohydrate metabolic process"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0019543","properties":[{"key":"GoTerm","value":"P:propionate catabolic process"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"9579066"}]},{"database":"GO","id":"GO:0006099","properties":[{"key":"GoTerm","value":"P:tricarboxylic acid cycle"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd06117","properties":[{"key":"EntryName","value":"Ec2MCS_like_1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.230.10:FF:000003","properties":[{"key":"EntryName","value":"Citrate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.580.10:FF:000004","properties":[{"key":"EntryName","value":"Citrate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.580.10","properties":[{"key":"EntryName","value":"Citrate Synthase, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.230.10","properties":[{"key":"EntryName","value":"Cytochrome P450-Terp, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR011278","properties":[{"key":"EntryName","value":"2-MeCitrate/Citrate_synth_II"}]},{"database":"InterPro","id":"IPR016142","properties":[{"key":"EntryName","value":"Citrate_synth-like_lrg_a-sub"}]},{"database":"InterPro","id":"IPR016143","properties":[{"key":"EntryName","value":"Citrate_synth-like_sm_a-sub"}]},{"database":"InterPro","id":"IPR002020","properties":[{"key":"EntryName","value":"Citrate_synthase"}]},{"database":"InterPro","id":"IPR019810","properties":[{"key":"EntryName","value":"Citrate_synthase_AS"}]},{"database":"InterPro","id":"IPR024176","properties":[{"key":"EntryName","value":"Citrate_synthase_bac-typ"}]},{"database":"InterPro","id":"IPR036969","properties":[{"key":"EntryName","value":"Citrate_synthase_sf"}]},{"database":"NCBIfam","id":"TIGR01800","properties":[{"key":"EntryName","value":"cit_synth_II"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009006","properties":[{"key":"EntryName","value":"PRK12351.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11739","properties":[{"key":"EntryName","value":"CITRATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11739:SF25","properties":[{"key":"EntryName","value":"CITRATE SYNTHASE-RELATED PROTEIN DDB_G0287281"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00285","properties":[{"key":"EntryName","value":"Citrate_synt"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF001369","properties":[{"key":"EntryName","value":"Citrate_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00143","properties":[{"key":"EntryName","value":"CITRTSNTHASE"}]},{"database":"SUPFAM","id":"SSF48256","properties":[{"key":"EntryName","value":"Citrate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00480","properties":[{"key":"EntryName","value":"CITRATE_SYNTHASE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHYRGYDILDLAKHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPAASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERIQPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSDMYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPVYTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNMMGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ","length":389,"molWeight":43102,"crc64":"02B779E7AD4581C3","md5":"D7CBD5CAF74A75301DA0D8B5CAE459F3"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":2,"SUBUNIT":1,"INDUCTION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Chain":1,"Active site":3,"Binding site":6},"uniParcId":"UPI0000132346"}}