{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P30137","secondaryAccessions":["Q2M8T0"],"uniProtkbId":"THIE_ECOLI","entryAudit":{"firstPublicDate":"1993-04-01","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"1993-04-01","entryVersion":187,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Thiamine-phosphate synthase"},"shortNames":[{"value":"TP synthase"},{"value":"TPS"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}],"value":"2.5.1.3"}]},"alternativeNames":[{"fullName":{"value":"Thiamine-phosphate pyrophosphorylase"},"shortNames":[{"value":"TMP pyrophosphorylase"},{"value":"TMP-PPase"}]}]},"genes":[{"geneName":{"value":"thiE"},"orderedLocusNames":[{"value":"b3993"},{"value":"JW3957"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15292217"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}],"value":"Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:47844"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:37575"},{"database":"ChEBI","id":"CHEBI:57841"},{"database":"ChEBI","id":"CHEBI:62899"}],"ecNumber":"2.5.1.3","evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:47848"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:37575"},{"database":"ChEBI","id":"CHEBI:57841"},{"database":"ChEBI","id":"CHEBI:62890"}],"ecNumber":"2.5.1.3","evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"4-methyl-5-(2-phosphooxyethyl)-thiazole + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + H(+) = thiamine phosphate + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:22328"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:37575"},{"database":"ChEBI","id":"CHEBI:57841"},{"database":"ChEBI","id":"CHEBI:58296"}],"ecNumber":"2.5.1.3","evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 1 Mg(2+) ion per subunit."}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":1.0,"unit":"uM","substrate":"HMP-PP","evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}]},{"constant":2.0,"unit":"uM","substrate":"THZ-P","evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}]}]}},{"texts":[{"value":"Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1"}],"commentType":"PATHWAY"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":23014.8,"molWeightError":0.0,"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10082377"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the thiamine-phosphate synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"Thiamine-phosphate synthase","featureId":"PRO_0000157010"},{"type":"Binding site","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57841"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine","id":"ChEBI:CHEBI:57841"}},{"type":"Binding site","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57841"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine","id":"ChEBI:CHEBI:57841"}},{"type":"Binding site","location":{"start":{"value":70,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":89,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":108,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57841"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine","id":"ChEBI:CHEBI:57841"}},{"type":"Binding site","location":{"start":{"value":134,"modifier":"EXACT"},"end":{"value":136,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:62899"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate","id":"ChEBI:CHEBI:62899"}},{"type":"Binding site","location":{"start":{"value":137,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57841"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine","id":"ChEBI:CHEBI:57841"}},{"type":"Binding site","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:62899"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate","id":"ChEBI:CHEBI:62899"}},{"type":"Binding site","location":{"start":{"value":186,"modifier":"EXACT"},"end":{"value":187,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:62899"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate","id":"ChEBI:CHEBI:62899"}}],"keywords":[{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0784","category":"Biological process","name":"Thiamine biosynthesis"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"8432721","citationType":"journal article","authors":["Vander Horn P.B.","Backstrom A.D.","Stewart V.","Begley T.P."],"citationCrossReferences":[{"database":"PubMed","id":"8432721"},{"database":"DOI","id":"10.1128/jb.175.4.982-992.1993"}],"title":"Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12.","publicationDate":"1993","journal":"J. Bacteriol.","firstPage":"982","lastPage":"992","volume":"175"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"K12","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"8265357","citationType":"journal article","authors":["Blattner F.R.","Burland V.D.","Plunkett G. III","Sofia H.J.","Daniels D.L."],"citationCrossReferences":[{"database":"PubMed","id":"8265357"},{"database":"DOI","id":"10.1093/nar/21.23.5408"}],"title":"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.","publicationDate":"1993","journal":"Nucleic Acids Res.","firstPage":"5408","lastPage":"5417","volume":"21"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-1BM49UEH13HSC","citationType":"journal article","authors":["Backstrom A.D.","Austin R.","McMordie S.","Begley T.P."],"title":"Biosynthesis of thiamin I: the function of the thiE gene product.","publicationDate":"1995","journal":"J. Am. Chem. Soc.","firstPage":"2351","lastPage":"2352","volume":"117"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","KINETIC PARAMETERS"]},{"referenceNumber":6,"citation":{"id":"10082377","citationType":"journal article","authors":["Kelleher N.L.","Taylor S.V.","Grannis D.","Kinsland C.","Chiu H.-J.","Begley T.P.","McLafferty F.W."],"citationCrossReferences":[{"database":"PubMed","id":"10082377"},{"database":"DOI","id":"10.1002/pro.5560070815"}],"title":"Efficient sequence analysis of the six gene products (7-74 kDa) from the Escherichia coli thiamin biosynthetic operon by tandem high-resolution mass spectrometry.","publicationDate":"1998","journal":"Protein Sci.","firstPage":"1796","lastPage":"1801","volume":"7"},"referencePositions":["MASS SPECTROMETRY"]},{"referenceNumber":7,"citation":{"id":"15292217","citationType":"journal article","authors":["Lawhorn B.G.","Gerdes S.Y.","Begley T.P."],"citationCrossReferences":[{"database":"PubMed","id":"15292217"},{"database":"DOI","id":"10.1074/jbc.m404284200"}],"title":"A genetic screen for the identification of thiamin metabolic genes.","publicationDate":"2004","journal":"J. Biol. Chem.","firstPage":"43555","lastPage":"43559","volume":"279"},"referencePositions":["FUNCTION IN THIAMINE METABOLISM"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"M88701","properties":[{"key":"ProteinId","value":"AAB95617.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00006","properties":[{"key":"ProteinId","value":"AAC43091.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC76967.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE77326.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S35118","properties":[{"key":"EntryName","value":"S35118"}]},{"database":"RefSeq","id":"NP_418421.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000284615.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P30137","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P30137","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4259333","properties":[{"key":"Interactions","value":"22"}]},{"database":"DIP","id":"DIP-10983N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P30137","properties":[{"key":"Number of interactors","value":"658"}]},{"database":"IntAct","id":"P30137","properties":[{"key":"Interactions","value":"4"}]},{"database":"STRING","id":"511145.b3993","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b3993","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC76967","properties":[{"key":"ProteinId","value":"AAC76967"},{"key":"GeneId","value":"b3993"}]},{"database":"GeneID","id":"75205511","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"948491","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW3957","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b3993","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_21570","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.2718","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB1545","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0352","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_018272_3_3_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P30137","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"QDFYHIK","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PhylomeDB","id":"P30137","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:THIE-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:THIE-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"SABIO-RK","id":"P30137","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00060","properties":[{"key":"RectionId","value":"UER00141"}]},{"database":"PRO","id":"PR:P30137","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"4580841"}]},{"database":"GO","id":"GO:0046872","properties":[{"key":"GoTerm","value":"F:metal ion binding"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"4580841"}]},{"database":"GO","id":"GO:0004789","properties":[{"key":"GoTerm","value":"F:thiamine-phosphate diphosphorylase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0009228","properties":[{"key":"GoTerm","value":"P:thiamine biosynthetic process"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"10382260"}]},{"database":"GO","id":"GO:0009229","properties":[{"key":"GoTerm","value":"P:thiamine diphosphate biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd00564","properties":[{"key":"EntryName","value":"TMP_TenI"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.70:FF:000064","properties":[{"key":"EntryName","value":"Thiamine-phosphate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00097","properties":[{"key":"EntryName","value":"TMP_synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR036206","properties":[{"key":"EntryName","value":"ThiamineP_synth_sf"}]},{"database":"InterPro","id":"IPR022998","properties":[{"key":"EntryName","value":"ThiamineP_synth_TenI"}]},{"database":"InterPro","id":"IPR034291","properties":[{"key":"EntryName","value":"TMP_synthase"}]},{"database":"NCBIfam","id":"NF002904","properties":[{"key":"EntryName","value":"PRK03512.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00693","properties":[{"key":"EntryName","value":"thiE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR20857","properties":[{"key":"EntryName","value":"THIAMINE-PHOSPHATE PYROPHOSPHORYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR20857:SF15","properties":[{"key":"EntryName","value":"THIAMINE-PHOSPHATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02581","properties":[{"key":"EntryName","value":"TMP-TENI"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51391","properties":[{"key":"EntryName","value":"Thiamin phosphate synthase"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MYQPDFPPVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRRDEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAGLRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYPTVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE","length":211,"molWeight":23015,"crc64":"C731953E46BC33E1","md5":"24082B5FE5A87C8B67F7CF654AA084DB"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":3,"COFACTOR":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"MASS SPECTROMETRY":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":9},"uniParcId":"UPI0000136DCF"}}