{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P28320","secondaryAccessions":["D6VXJ3"],"uniProtkbId":"YJU2_YEAST","entryAudit":{"firstPublicDate":"1992-12-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1992-12-01","entryVersion":175,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03226"}],"value":"Splicing factor YJU2"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03226"}],"value":"YJU2"},"synonyms":[{"value":"CWC16"}],"orderedLocusNames":[{"value":"YKL095W"}],"orfNames":[{"value":"YKL442"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03226"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19935684"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23438600"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27445308"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27459055"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29146871"}],"value":"Part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA (PubMed:19935684, PubMed:27445308, PubMed:27459055, PubMed:29146871). Plays a role (via N-terminus) in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'-intermediates (PubMed:23438600, PubMed:27459055). Further stabilizes spliceosome conformation for 3'-splice site docking (via C-terminus) promoting exon ligation (PubMed:29146871)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03226"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11884590"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19935684"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23438600"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27445308"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27459055"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29146871"}],"value":"Component of the spliceosome. Present in the activated B complex, the catalytically activated B* complex which catalyzes the branching, the catalytic step 1 C complex catalyzing the exon ligation, and the postcatalytic P complex containing the ligated exons (mRNA) and the excised lariat intron (PubMed:19935684, PubMed:27445308, PubMed:27459055, PubMed:29146871). Interacts (via C-terminus) with CLF1 (PubMed:23438600). Interacts (via N-terminus) with SYF1 (PubMed:23438600). Interacts with U2 snRNA; this interaction is direct (PubMed:23438600). Identified in the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2 (PubMed:11884590)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P28320","intActId":"EBI-26795"},"interactantTwo":{"uniProtKBAccession":"Q04048","geneName":"SYF1","intActId":"EBI-540"},"numberOfExperiments":3,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03226"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562095"}],"value":"Nucleus","id":"SL-0191"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1626433"}],"value":"Expressed in exponential-phase cells grown in rich medium"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562106"}],"value":"Present with 2250 molecules/cell in log phase SD medium"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03226"}],"value":"Belongs to the CWC16 family. YJU2 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":278,"modifier":"EXACT"}},"description":"Splicing factor YJU2","featureId":"PRO_0000203163"},{"type":"Region","location":{"start":{"value":228,"modifier":"EXACT"},"end":{"value":278,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Motif","location":{"start":{"value":242,"modifier":"EXACT"},"end":{"value":258,"modifier":"EXACT"}},"description":"Nuclear localization signal","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"1626433"}]},{"type":"Motif","location":{"start":{"value":260,"modifier":"EXACT"},"end":{"value":278,"modifier":"EXACT"}},"description":"Nuclear localization signal","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"1626433"}]},{"type":"Binding site","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03226"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27445308"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27459055"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03226"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27445308"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27459055"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03226"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27445308"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27459055"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":91,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03226"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27445308"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27459055"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Helix","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":30,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5GMK"}]},{"type":"Beta strand","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5GMK"}]},{"type":"Beta strand","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5GMK"}]},{"type":"Beta strand","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5GMK"}]},{"type":"Beta strand","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5GMK"}]},{"type":"Beta strand","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":78,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5GMK"}]},{"type":"Beta strand","location":{"start":{"value":82,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5GMK"}]},{"type":"Beta strand","location":{"start":{"value":89,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5GMK"}]},{"type":"Beta strand","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5GMK"}]},{"type":"Beta strand","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5GMK"}]},{"type":"Helix","location":{"start":{"value":167,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y88"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0507","category":"Biological process","name":"mRNA processing"},{"id":"KW-0508","category":"Biological process","name":"mRNA splicing"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0747","category":"Cellular component","name":"Spliceosome"},{"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"1626433","citationType":"journal article","authors":["Forrova H.","Kolarov J.","Ghislain M.","Goffeau A."],"citationCrossReferences":[{"database":"PubMed","id":"1626433"},{"database":"DOI","id":"10.1002/yea.320080509"}],"title":"Sequence of the novel essential gene YJU2 and two flanking reading frames located within a 3.2 kb EcoRI fragment from chromosome X of Saccharomyces cerevisiae.","publicationDate":"1992","journal":"Yeast","firstPage":"419","lastPage":"422","volume":"8"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","INDUCTION","MOTIF"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"8256524","citationType":"journal article","authors":["Pallier C.","Valens M.","Puzos V.","Fukuhara H.","Cheret G.","Sor F.","Bolotin-Fukuhara M."],"citationCrossReferences":[{"database":"PubMed","id":"8256524"},{"database":"DOI","id":"10.1002/yea.320091016"}],"title":"DNA sequence analysis of a 17 kb fragment of yeast chromosome XI physically localizes the MRB1 gene and reveals eight new open reading frames, including a homologue of the KIN1/KIN2 and SNF1 protein kinases.","publicationDate":"1993","journal":"Yeast","firstPage":"1149","lastPage":"1155","volume":"9"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"8196765","citationType":"journal article","authors":["Dujon B.","Alexandraki D.","Andre B.","Ansorge W.","Baladron V.","Ballesta J.P.G.","Banrevi A.","Bolle P.-A.","Bolotin-Fukuhara M.","Bossier P.","Bou G.","Boyer J.","Buitrago M.J.","Cheret G.","Colleaux L.","Daignan-Fornier B.","del Rey F.","Dion C.","Domdey H.","Duesterhoeft A.","Duesterhus S.","Entian K.-D.","Erfle H.","Esteban P.F.","Feldmann H.","Fernandes L.","Fobo G.M.","Fritz C.","Fukuhara H.","Gabel C.","Gaillon L.","Garcia-Cantalejo J.M.","Garcia-Ramirez J.J.","Gent M.E.","Ghazvini M.","Goffeau A.","Gonzalez A.","Grothues D.","Guerreiro P.","Hegemann J.H.","Hewitt N.","Hilger F.","Hollenberg C.P.","Horaitis O.","Indge K.J.","Jacquier A.","James C.M.","Jauniaux J.-C.","Jimenez A.","Keuchel H.","Kirchrath L.","Kleine K.","Koetter P.","Legrain P.","Liebl S.","Louis E.J.","Maia e Silva A.","Marck C.","Monnier A.-L.","Moestl D.","Mueller S.","Obermaier B.","Oliver S.G.","Pallier C.","Pascolo S.","Pfeiffer F.","Philippsen P.","Planta R.J.","Pohl F.M.","Pohl T.M.","Poehlmann R.","Portetelle D.","Purnelle B.","Puzos V.","Ramezani Rad M.","Rasmussen S.W.","Remacha M.A.","Revuelta J.L.","Richard G.-F.","Rieger M.","Rodrigues-Pousada C.","Rose M.","Rupp T.","Santos M.A.","Schwager C.","Sensen C.","Skala J.","Soares H.","Sor F.","Stegemann J.","Tettelin H.","Thierry A.","Tzermia M.","Urrestarazu L.A.","van Dyck L.","van Vliet-Reedijk J.C.","Valens M.","Vandenbol M.","Vilela C.","Vissers S.","von Wettstein D.","Voss H.","Wiemann S.","Xu G.","Zimmermann J.","Haasemann M.","Becker I.","Mewes H.-W."],"citationCrossReferences":[{"database":"PubMed","id":"8196765"},{"database":"DOI","id":"10.1038/369371a0"}],"title":"Complete DNA sequence of yeast chromosome XI.","publicationDate":"1994","journal":"Nature","firstPage":"371","lastPage":"378","volume":"369"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"11884590","citationType":"journal article","authors":["Ohi M.D.","Link A.J.","Ren L.","Jennings J.L.","McDonald W.H.","Gould K.L."],"citationCrossReferences":[{"database":"PubMed","id":"11884590"},{"database":"DOI","id":"10.1128/mcb.22.7.2011-2024.2002"}],"title":"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs.","publicationDate":"2002","journal":"Mol. Cell. Biol.","firstPage":"2011","lastPage":"2024","volume":"22"},"referencePositions":["IDENTIFICATION IN THE CWC COMPLEX","IDENTIFICATION BY MASS SPECTROMETRY"]},{"referenceNumber":6,"citation":{"id":"14690591","citationType":"journal article","authors":["Hazbun T.R.","Malmstroem L.","Anderson S.","Graczyk B.J.","Fox B.","Riffle M.","Sundin B.A.","Aranda J.D.","McDonald W.H.","Chiu C.-H.","Snydsman B.E.","Bradley P.","Muller E.G.D.","Fields S.","Baker D.","Yates J.R. III","Davis T.N."],"citationCrossReferences":[{"database":"PubMed","id":"14690591"},{"database":"DOI","id":"10.1016/s1097-2765(03)00476-3"}],"title":"Assigning function to yeast proteins by integration of technologies.","publicationDate":"2003","journal":"Mol. Cell","firstPage":"1353","lastPage":"1365","volume":"12"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY"]},{"referenceNumber":7,"citation":{"id":"14562095","citationType":"journal article","authors":["Huh W.-K.","Falvo J.V.","Gerke L.C.","Carroll A.S.","Howson R.W.","Weissman J.S.","O'Shea E.K."],"citationCrossReferences":[{"database":"PubMed","id":"14562095"},{"database":"DOI","id":"10.1038/nature02026"}],"title":"Global analysis of protein localization in budding yeast.","publicationDate":"2003","journal":"Nature","firstPage":"686","lastPage":"691","volume":"425"},"referencePositions":["SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":8,"citation":{"id":"14562106","citationType":"journal article","authors":["Ghaemmaghami S.","Huh W.-K.","Bower K.","Howson R.W.","Belle A.","Dephoure N.","O'Shea E.K.","Weissman J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14562106"},{"database":"DOI","id":"10.1038/nature02046"}],"title":"Global analysis of protein expression in yeast.","publicationDate":"2003","journal":"Nature","firstPage":"737","lastPage":"741","volume":"425"},"referencePositions":["LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":9,"citation":{"id":"19935684","citationType":"journal article","authors":["Warkocki Z.","Odenwaelder P.","Schmitzova J.","Platzmann F.","Stark H.","Urlaub H.","Ficner R.","Fabrizio P.","Luehrmann R."],"citationCrossReferences":[{"database":"PubMed","id":"19935684"},{"database":"DOI","id":"10.1038/nsmb.1729"}],"title":"Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components.","publicationDate":"2009","journal":"Nat. Struct. Mol. Biol.","firstPage":"1237","lastPage":"1243","volume":"16"},"referencePositions":["IDENTIFICATION IN THE ACTIVATED B COMPLEX","IDENTIFICATION BY MASS SPECTROMETRY","FUNCTION"]},{"referenceNumber":10,"citation":{"id":"23438600","citationType":"journal article","authors":["Chiang T.W.","Cheng S.C."],"citationCrossReferences":[{"database":"PubMed","id":"23438600"},{"database":"DOI","id":"10.1128/mcb.00035-13"}],"title":"A weak spliceosome-binding domain of Yju2 functions in the first step and bypasses Prp16 in the second step of splicing.","publicationDate":"2013","journal":"Mol. Cell. Biol.","firstPage":"1746","lastPage":"1755","volume":"33"},"referencePositions":["FUNCTION","INTERACTION WITH SYF1","INTERACTION WITH CLF1"]},{"referenceNumber":11,"citation":{"id":"27459055","citationType":"journal article","authors":["Galej W.P.","Wilkinson M.E.","Fica S.M.","Oubridge C.","Newman A.J.","Nagai K."],"citationCrossReferences":[{"database":"PubMed","id":"27459055"},{"database":"DOI","id":"10.1038/nature19316"}],"title":"Cryo-EM structure of the spliceosome immediately after branching.","publicationDate":"2016","journal":"Nature","firstPage":"197","lastPage":"201","volume":"537"},"referencePositions":["STRUCTURE BY ELECTRON MICROSCOPY (3.80 ANGSTROMS) IN COMPLEX WITH ZINC IONS","FUNCTION","IDENTIFICATION IN C COMPLEX","IDENTIFICATION BY MASS SPECTROMETRY"]},{"referenceNumber":12,"citation":{"id":"27445308","citationType":"journal article","authors":["Wan R.","Yan C.","Bai R.","Huang G.","Shi Y."],"citationCrossReferences":[{"database":"PubMed","id":"27445308"},{"database":"DOI","id":"10.1126/science.aag2235"}],"title":"Structure of a yeast catalytic step I spliceosome at 3.4 A resolution.","publicationDate":"2016","journal":"Science","firstPage":"895","lastPage":"904","volume":"353"},"referencePositions":["STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS) IN COMPLEX WITH ZINC IONS","FUNCTION","IDENTIFICATION IN C COMPLEX"]},{"referenceNumber":13,"citation":{"id":"29146871","citationType":"journal article","authors":["Wilkinson M.E.","Fica S.M.","Galej W.P.","Norman C.M.","Newman A.J.","Nagai K."],"citationCrossReferences":[{"database":"PubMed","id":"29146871"},{"database":"DOI","id":"10.1126/science.aar3729"}],"title":"Postcatalytic spliceosome structure reveals mechanism of 3'-splice site selection.","publicationDate":"2017","journal":"Science","firstPage":"1283","lastPage":"1288","volume":"358"},"referencePositions":["STRUCTURE BY ELECTRON MICROSCOPY (3.70 ANGSTROMS)","FUNCTION","IDENTIFICATION IN P COMPLEX","IDENTIFICATION BY MASS SPECTROMETRY"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X66245","properties":[{"key":"ProteinId","value":"CAA46970.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"X71133","properties":[{"key":"ProteinId","value":"CAA50462.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"Z28095","properties":[{"key":"ProteinId","value":"CAA81933.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006944","properties":[{"key":"ProteinId","value":"DAA09063.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S25355","properties":[{"key":"EntryName","value":"S25355"}]},{"database":"RefSeq","id":"NP_012828.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001179661.1"}]},{"database":"PDB","id":"5GMK","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.40 A"},{"key":"Chains","value":"F=1-278"}]},{"database":"PDB","id":"5LJ3","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.80 A"},{"key":"Chains","value":"D=1-278"}]},{"database":"PDB","id":"5LJ5","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.80 A"},{"key":"Chains","value":"D=1-278"}]},{"database":"PDB","id":"5Y88","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.70 A"},{"key":"Chains","value":"R=1-278"}]},{"database":"PDB","id":"6EXN","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.70 A"},{"key":"Chains","value":"D=1-278"}]},{"database":"PDB","id":"6J6Q","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.70 A"},{"key":"Chains","value":"F=1-278"}]},{"database":"PDBsum","id":"5GMK","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5LJ3","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5LJ5","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5Y88","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"6EXN","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"6J6Q","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P28320","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-0692","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-3979","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-4055","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-4057","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-6817","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-9525","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P28320","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"34038","properties":[{"key":"Interactions","value":"388"}]},{"database":"ComplexPortal","id":"CPX-1651","properties":[{"key":"EntryName","value":"PRP19-associated complex"}]},{"database":"DIP","id":"DIP-1915N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P28320","properties":[{"key":"Number of interactors","value":"819"}]},{"database":"IntAct","id":"P28320","properties":[{"key":"Interactions","value":"76"}]},{"database":"MINT","id":"P28320","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"4932.YKL095W","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YKL095W","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P28320","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YKL095W_mRNA","properties":[{"key":"ProteinId","value":"YKL095W"},{"key":"GeneId","value":"YKL095W"}]},{"database":"GeneID","id":"853767","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YKL095W","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000001578","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000001578","properties":[{"key":"GeneName","value":"YJU2"}]},{"database":"VEuPathDB","id":"FungiDB:YKL095W","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG2989","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_053603_2_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P28320","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"NNDYQCE","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"674963at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-31886-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"853767","properties":[{"key":"hits","value":"0 hits in 10 CRISPR screens"}]},{"database":"PRO","id":"PR:P28320","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome XI"}]},{"database":"RNAct","id":"P28320","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"14562095"}]},{"database":"GO","id":"GO:0000974","properties":[{"key":"GoTerm","value":"C:Prp19 complex"},{"key":"GoEvidenceType","value":"IPI:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"11884590"}]},{"database":"GO","id":"GO:0071006","properties":[{"key":"GoTerm","value":"C:U2-type catalytic step 1 spliceosome"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23438600"}]},{"database":"GO","id":"GO:0071007","properties":[{"key":"GoTerm","value":"C:U2-type catalytic step 2 spliceosome"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23438600"}]},{"database":"GO","id":"GO:0030620","properties":[{"key":"GoTerm","value":"F:U2 snRNA binding"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23438600"}]},{"database":"GO","id":"GO:0000349","properties":[{"key":"GoTerm","value":"P:generation of catalytic spliceosome for first transesterification step"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23438600"}]},{"database":"GO","id":"GO:0000350","properties":[{"key":"GoTerm","value":"P:generation of catalytic spliceosome for second transesterification step"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23438600"}]},{"database":"GO","id":"GO:0045292","properties":[{"key":"GoTerm","value":"P:mRNA cis splicing, via spliceosome"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0000398","properties":[{"key":"GoTerm","value":"P:mRNA splicing, via spliceosome"},{"key":"GoEvidenceType","value":"NAS:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23742842"}]},{"database":"HAMAP","id":"MF_03226","properties":[{"key":"EntryName","value":"YJU2"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR007590","properties":[{"key":"EntryName","value":"Saf4/Yju2"}]},{"database":"InterPro","id":"IPR043701","properties":[{"key":"EntryName","value":"Yju2"}]},{"database":"PANTHER","id":"PTHR12111","properties":[{"key":"EntryName","value":"SPLICING FACTOR YJU2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12111:SF1","properties":[{"key":"EntryName","value":"SPLICING FACTOR YJU2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04502","properties":[{"key":"EntryName","value":"Saf4_Yju2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSERKAINKYYPPDYNPLEAEKLSRKMAKKLKTMNKSHASIRLMTPFSMRCLECNEYIPKSRKFNGKKELLKEKYLDSIKIYRLTISCPRCANSIAFRTDPGNSDYVMEVGGVRNYVPQKPNDDLNAKTAVESIDETLQRLVREKEMEQNEKMGIKEQADDKMDLLEKRLAKIQQEQEDDEELENLRKKNLEMSQRAEMINRSKHAQQEKAVTTDDLDNLVDQVFDNHRQRTNKPGNNNDEKRTPLFNPTSTKGKIQKKSSVRTNPLGIVIKRGKSLK","length":278,"molWeight":32312,"crc64":"B57F3A173A2113FD","md5":"D41FB18787AF7C5935DA0D4D63114A87"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":1,"Motif":2,"Binding site":4,"Helix":2,"Beta strand":9},"uniParcId":"UPI00000531F4"}}