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Both reactions are activated by ATP and 2-ketoglutarate, via the binding of these effector molecules to the substrates PII and PII-UMP"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":3.0,"unit":"uM","substrate":"GlnB protein","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9737855"}]},{"constant":40.0,"unit":"uM","substrate":"UTP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9737855"}]},{"constant":2.3,"unit":"uM","substrate":"uridylyl-[protein-PII] (in the absence of glutamine)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9737855"}]},{"constant":0.82,"unit":"uM","substrate":"uridylyl-[protein-PII] (in the presence of 2.5 mM glutamine)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9737855"}]}],"note":{"texts":[{"value":"kcat is 137 min(-1) for the UTase reaction. kcat is 2.7 min(-1) for the UR reaction in the absence of glutamine, and 6.5 min(-1) in the presence of 2.5 mM glutamine."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"6130097"}],"value":"Monomer. 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The uridylyl-removing reaction proceeds with rapid equilibrium binding of substrate and random release of products (PubMed:9737855)"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00277"}],"value":"Belongs to the GlnD family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":890,"modifier":"EXACT"}},"description":"Bifunctional uridylyltransferase/uridylyl-removing enzyme","featureId":"PRO_0000192732"},{"type":"Domain","location":{"start":{"value":468,"modifier":"EXACT"},"end":{"value":590,"modifier":"EXACT"}},"description":"HD","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01175"}]},{"type":"Domain","location":{"start":{"value":709,"modifier":"EXACT"},"end":{"value":789,"modifier":"EXACT"}},"description":"ACT 1","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00277"}]},{"type":"Domain","location":{"start":{"value":816,"modifier":"EXACT"},"end":{"value":890,"modifier":"EXACT"}},"description":"ACT 2","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00277"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":349,"modifier":"EXACT"}},"description":"Uridylyltransferase"},{"type":"Region","location":{"start":{"value":350,"modifier":"EXACT"},"end":{"value":708,"modifier":"EXACT"}},"description":"Uridylyl-removing"},{"type":"Mutagenesis","location":{"start":{"value":93,"modifier":"EXACT"},"end":{"value":93,"modifier":"EXACT"}},"description":"Loss of UTase activity, while no significant effect on UR activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20363937"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["L","V"]}},{"type":"Mutagenesis","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":94,"modifier":"EXACT"}},"description":"Loss of UTase activity, while no significant effect on UR activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20363937"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["D"]}},{"type":"Mutagenesis","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"Loss of UTase activity, while no significant effect on UR activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20363937"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A","V","Y"]}},{"type":"Mutagenesis","location":{"start":{"value":514,"modifier":"EXACT"},"end":{"value":515,"modifier":"EXACT"}},"description":"Loss of UR activity, while no significant effect on UTase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20363937"}],"alternativeSequence":{"originalSequence":"HD","alternativeSequences":["AA","QN"]}},{"type":"Sequence conflict","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":47,"modifier":"EXACT"}},"description":"in Ref. 1; 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