{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P27189","secondaryAccessions":["P27184"],"uniProtkbId":"TRAC4_ECOLX","entryAudit":{"firstPublicDate":"1992-08-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1992-08-01","entryVersion":95,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Escherichia coli","taxonId":562,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"DNA primase TraC"},"ecNumbers":[{"value":"2.7.7.-"}]},"alternativeNames":[{"fullName":{"value":"Replication primase"}}]},"genes":[{"geneName":{"value":"traC"}}],"comments":[{"texts":[{"value":"Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single-stranded template DNA"}],"commentType":"FUNCTION"},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative initiation"],"isoforms":[{"name":{"value":"TraC-1"},"isoformIds":["P27189-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"TraC-2"},"isoformIds":["P27189-2"],"sequenceIds":["VSP_018716"],"isoformSequenceStatus":"Described"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1061,"modifier":"EXACT"}},"description":"DNA primase TraC","featureId":"PRO_0000007251"},{"type":"Domain","location":{"start":{"value":850,"modifier":"EXACT"},"end":{"value":938,"modifier":"EXACT"}},"description":"Toprim","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00995"}]},{"type":"Region","location":{"start":{"value":1034,"modifier":"EXACT"},"end":{"value":1061,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Alternative sequence","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":315,"modifier":"EXACT"}},"description":"in isoform TraC-2","evidences":[{"evidenceCode":"ECO:0000305"}],"featureId":"VSP_018716","alternativeSequence":{}}],"geneLocations":[{"value":"IncP-alpha RP4","geneEncodingType":"Plasmid"}],"keywords":[{"id":"KW-0024","category":"Coding sequence diversity","name":"Alternative initiation"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0235","category":"Biological process","name":"DNA replication"},{"id":"KW-0240","category":"Cellular component","name":"DNA-directed RNA polymerase"},{"id":"KW-0548","category":"Molecular function","name":"Nucleotidyltransferase"},{"id":"KW-0614","category":"Technical term","name":"Plasmid"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"1818755","citationType":"journal article","authors":["Miele L.","Strack B.","Kruft V.","Lanka E."],"citationCrossReferences":[{"database":"PubMed","id":"1818755"},{"database":"DOI","id":"10.3109/10425179109039685"}],"title":"Gene organization and nucleotide sequence of the primase region of IncP plasmids RP4 and R751.","publicationDate":"1991","journal":"DNA Seq.","firstPage":"145","lastPage":"162","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","PROTEIN SEQUENCE OF 1-14 AND 316-333"],"referenceComments":[{"value":"ATCC 33694 / HB101","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X59793","properties":[{"key":"ProteinId","value":"CAA42455.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"X59793","properties":[{"key":"ProteinId","value":"CAA42456.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S37667","properties":[{"key":"EntryName","value":"S37667"}]},{"database":"RefSeq","id":"WP_011205813.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_JAVAIN010000003.1"}],"isoformId":"P27189-1"},{"database":"AlphaFoldDB","id":"P27189","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P27189","properties":[{"key":"Description","value":"-"}]},{"database":"BindingDB","id":"P27189","properties":[{"key":"Description","value":"-"}]},{"database":"ChEMBL","id":"CHEMBL1075082","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0003697","properties":[{"key":"GoTerm","value":"F:single-stranded DNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd01029","properties":[{"key":"EntryName","value":"TOPRIM_primases"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR013610","properties":[{"key":"EntryName","value":"ArdC_N"}]},{"database":"InterPro","id":"IPR043764","properties":[{"key":"EntryName","value":"DUF5710"}]},{"database":"InterPro","id":"IPR041459","properties":[{"key":"EntryName","value":"MPTase-PolyVal"}]},{"database":"InterPro","id":"IPR034154","properties":[{"key":"EntryName","value":"TOPRIM_DnaG/twinkle"}]},{"database":"InterPro","id":"IPR006171","properties":[{"key":"EntryName","value":"TOPRIM_dom"}]},{"database":"Pfam","id":"PF08401","properties":[{"key":"EntryName","value":"ArdcN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF18974","properties":[{"key":"EntryName","value":"DUF5710"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF18818","properties":[{"key":"EntryName","value":"MPTase-PolyVal"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13362","properties":[{"key":"EntryName","value":"Toprim_3"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00493","properties":[{"key":"EntryName","value":"TOPRIM"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50880","properties":[{"key":"EntryName","value":"TOPRIM"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAEVKKPFHEQVAERLIEQLKAGTAPWQKPWEPGMPGSFIPLNPTTGKRYKGINAIQLMAQGHADPRWMTYKQAAAAGAQVRRGEKGTPIQYWKFSEEQTKTDEQTGKPVLDANGDPVKVTVQLERPRVFFATVFNAEQIDGLPPLERKEQTWSAVERAEHILAASGATIRHGEHDRAFYRPSTDSIHLPDKGQFPSADNYYATALHELGHWTGHPSRLDRDLAHPFGSEGYAKEELRAEIASMILGDELGIGHDPGQHAAYVGSWIKALQEDPLEIFRAAADAEKIQDFVLAFEQKQIQEQTTQQAIEPAQGATMEQQQDQVARPAIAPADELIAQTLRMYRAGAEPAEGNQSLAALTETTLGFELPADWTGRVQVQANVEVEHDGERSVVPAGDREPEFWGVYANHAWGGHQWLADFAGPDAQTNAEALADRLAVIDAYATANEYEQAAKFARIHEERVRRDPNSTDEDRVAAKEARKAAEGTAMLHDEDLQRRIADYEREQQEMAQAMNAAEQPAAAQAPAKPERAYLNVPFKEKDEVKALGARWDRQERAWYVPAGVDPAPFAKWAREGATAAVEARAEAQPTQPTAERPNAAQERVYLAVPYGERQVAKAAGAQWDKVAKSWYAGPNADMGKLQRWLPDNVPTQQSPAVTPEDEFAEALKSMGCVVTPGGEHPIMDGKKHRIETEGDKKGEKSGFYVGHLDGHPAGYIKNNRTGVEMKWKAKGYALDPAEKAKMQAEAAAKLAARAEEQERQHEATAQRIGRQAQSLVPITEPTPYLRDKGLQVHAGVLTDQEGQKTYIPAYDADGKQWTMQYIQEDGTKRFAKDSRKEGCFHVVGGMDALAAAPALVIGEGYATAATVAEALGHATVAAFDSGNLQAVAEALHAKFPDKPVVIAGDDDRQVQITQGVNPGRTKAQEAAKAVGGKAIFPIFAPGENAYPKELPPITPENYRNHLHAEKRLADAAAGKVQLSEADTAKLKESLLNDGQLAALSNMKKHTDFNDLSERSSLGKDGVERQVRSAVGKVLLDEGQRQKVQQLKQQDIEQQEQRQRRARTY","length":1061,"molWeight":116722,"crc64":"ABC344D2811B9B31","md5":"80E30E7CE78EC80F0C3C6122D2681279"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"ALTERNATIVE PRODUCTS":2},"countByFeatureType":{"Chain":1,"Domain":1,"Region":1,"Alternative sequence":1},"uniParcId":"UPI0000137336"}}