{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P26370","secondaryAccessions":["D6VRI1"],"uniProtkbId":"UGA3_YEAST","entryAudit":{"firstPublicDate":"1992-08-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1992-08-01","entryVersion":179,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Transcriptional activator protein UGA3"}}},"genes":[{"geneName":{"value":"UGA3"},"orderedLocusNames":[{"value":"YDL170W"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2109179"}],"value":"GABA-dependent positive regulation of genes required for catabolism of GABA (UGA4, UGA1, and UGA2)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2109179"}],"value":"UGA3 proteins associate in oligomers, at least in the presence of inducer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2109179"}],"value":"Nucleus","id":"SL-0191"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562106"}],"value":"Present with 1200 molecules/cell in log phase SD medium"}],"commentType":"MISCELLANEOUS"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":528,"modifier":"EXACT"}},"description":"Transcriptional activator protein UGA3","featureId":"PRO_0000114986"},{"type":"DNA binding","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"Zn(2)-C6 fungal-type","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00227"}]},{"type":"Motif","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"Nuclear localization signal","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Mutagenesis","location":{"start":{"value":224,"modifier":"EXACT"},"end":{"value":224,"modifier":"EXACT"}},"description":"Constitutive activity; when associated with R-451.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2109179"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["R"]}},{"type":"Mutagenesis","location":{"start":{"value":451,"modifier":"EXACT"},"end":{"value":451,"modifier":"EXACT"}},"description":"Loss of activity. Constitutive activity; when associated with R-224.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2109179"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["R"]}}],"keywords":[{"id":"KW-0010","category":"Molecular function","name":"Activator"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"},{"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"2109179","citationType":"journal article","authors":["Andre B."],"citationCrossReferences":[{"database":"PubMed","id":"2109179"},{"database":"DOI","id":"10.1007/bf00260493"}],"title":"The UGA3 gene regulating the GABA catabolic pathway in Saccharomyces cerevisiae codes for a putative zinc-finger protein acting on RNA amount.","publicationDate":"1990","journal":"Mol. Gen. Genet.","firstPage":"269","lastPage":"276","volume":"220"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION","SUBCELLULAR LOCATION","SUBUNIT","MUTAGENESIS OF GLY-224 AND GLY-451"],"referenceComments":[{"value":"Sigma 1278B","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9169867","citationType":"journal article","authors":["Jacq C.","Alt-Moerbe J.","Andre B.","Arnold W.","Bahr A.","Ballesta J.P.G.","Bargues M.","Baron L.","Becker A.","Biteau N.","Bloecker H.","Blugeon C.","Boskovic J.","Brandt P.","Brueckner M.","Buitrago M.J.","Coster F.","Delaveau T.","del Rey F.","Dujon B.","Eide L.G.","Garcia-Cantalejo J.M.","Goffeau A.","Gomez-Peris A.","Granotier C.","Hanemann V.","Hankeln T.","Hoheisel J.D.","Jaeger W.","Jimenez A.","Jonniaux J.-L.","Kraemer C.","Kuester H.","Laamanen P.","Legros Y.","Louis E.J.","Moeller-Rieker S.","Monnet A.","Moro M.","Mueller-Auer S.","Nussbaumer B.","Paricio N.","Paulin L.","Perea J.","Perez-Alonso M.","Perez-Ortin J.E.","Pohl T.M.","Prydz H.","Purnelle B.","Rasmussen S.W.","Remacha M.A.","Revuelta J.L.","Rieger M.","Salom D.","Saluz H.P.","Saiz J.E.","Saren A.-M.","Schaefer M.","Scharfe M.","Schmidt E.R.","Schneider C.","Scholler P.","Schwarz S.","Soler-Mira A.","Urrestarazu L.A.","Verhasselt P.","Vissers S.","Voet M.","Volckaert G.","Wagner G.","Wambutt R.","Wedler E.","Wedler H.","Woelfl S.","Harris D.E.","Bowman S.","Brown D.","Churcher C.M.","Connor R.","Dedman K.","Gentles S.","Hamlin N.","Hunt S.","Jones L.","McDonald S.","Murphy L.D.","Niblett D.","Odell C.","Oliver K.","Rajandream M.A.","Richards C.","Shore L.","Walsh S.V.","Barrell B.G.","Dietrich F.S.","Mulligan J.T.","Allen E.","Araujo R.","Aviles E.","Berno A.","Carpenter J.","Chen E.","Cherry J.M.","Chung E.","Duncan M.","Hunicke-Smith S.","Hyman R.W.","Komp C.","Lashkari D.","Lew H.","Lin D.","Mosedale D.","Nakahara K.","Namath A.","Oefner P.","Oh C.","Petel F.X.","Roberts D.","Schramm S.","Schroeder M.","Shogren T.","Shroff N.","Winant A.","Yelton M.A.","Botstein D.","Davis R.W.","Johnston M.","Andrews S.","Brinkman R.","Cooper J.","Ding H.","Du Z.","Favello A.","Fulton L.","Gattung S.","Greco T.","Hallsworth K.","Hawkins J.","Hillier L.W.","Jier M.","Johnson D.","Johnston L.","Kirsten J.","Kucaba T.","Langston Y.","Latreille P.","Le T.","Mardis E.","Menezes S.","Miller N.","Nhan M.","Pauley A.","Peluso D.","Rifkin L.","Riles L.","Taich A.","Trevaskis E.","Vignati D.","Wilcox L.","Wohldman P.","Vaudin M.","Wilson R.","Waterston R.","Albermann K.","Hani J.","Heumann K.","Kleine K.","Mewes H.-W.","Zollner A.","Zaccaria P."],"citationCrossReferences":[{"database":"PubMed","id":"9169867"},{"database":"DOI","id":"10.1038/387s075"}],"title":"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.","publicationDate":"1997","journal":"Nature","firstPage":"75","lastPage":"78","volume":"387"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"CI-A7P9CQ3RSA76N","citationType":"submission","authors":["Lindner Z."],"publicationDate":"NOV-1994","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 453-528"],"referenceComments":[{"value":"ATCC 38626 / AH22 / NRRL Y-12843","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"14562106","citationType":"journal article","authors":["Ghaemmaghami S.","Huh W.-K.","Bower K.","Howson R.W.","Belle A.","Dephoure N.","O'Shea E.K.","Weissman J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14562106"},{"database":"DOI","id":"10.1038/nature02046"}],"title":"Global analysis of protein expression in yeast.","publicationDate":"2003","journal":"Nature","firstPage":"737","lastPage":"741","volume":"425"},"referencePositions":["LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X51664","properties":[{"key":"ProteinId","value":"CAA35976.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"Z67750","properties":[{"key":"ProteinId","value":"CAA91576.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"Z74218","properties":[{"key":"ProteinId","value":"CAA98744.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"X82846","properties":[{"key":"ProteinId","value":"CAA58049.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006938","properties":[{"key":"ProteinId","value":"DAA11691.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S14944","properties":[{"key":"EntryName","value":"S14944"}]},{"database":"RefSeq","id":"NP_010111.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001180230.1"}]},{"database":"AlphaFoldDB","id":"P26370","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P26370","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"31895","properties":[{"key":"Interactions","value":"85"}]},{"database":"FunCoup","id":"P26370","properties":[{"key":"Number of interactors","value":"379"}]},{"database":"STRING","id":"4932.YDL170W","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"P26370","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YDL170W","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P26370","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YDL170W_mRNA","properties":[{"key":"ProteinId","value":"YDL170W"},{"key":"GeneId","value":"YDL170W"}]},{"database":"GeneID","id":"851384","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YDL170W","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000002329","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000002329","properties":[{"key":"GeneName","value":"UGA3"}]},{"database":"VEuPathDB","id":"FungiDB:YDL170W","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502RIXM","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_015493_1_2_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P26370","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"MPGTIDP","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"5419315at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-29561-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"851384","properties":[{"key":"hits","value":"3 hits in 13 CRISPR screens"}]},{"database":"PRO","id":"PR:P26370","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome IV"}]},{"database":"RNAct","id":"P26370","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"19159085"}]},{"database":"GO","id":"GO:0001228","properties":[{"key":"GoTerm","value":"F:DNA-binding transcription activator activity, RNA polymerase II-specific"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21515579"}]},{"database":"GO","id":"GO:0003700","properties":[{"key":"GoTerm","value":"F:DNA-binding transcription factor activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0000978","properties":[{"key":"GoTerm","value":"F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12235130"}]},{"database":"GO","id":"GO:0000976","properties":[{"key":"GoTerm","value":"F:transcription cis-regulatory region binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0008270","properties":[{"key":"GoTerm","value":"F:zinc ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0019740","properties":[{"key":"GoTerm","value":"P:nitrogen utilization"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"7899074"}]},{"database":"GO","id":"GO:0045944","properties":[{"key":"GoTerm","value":"P:positive regulation of transcription by RNA polymerase II"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"7899074"}]},{"database":"CDD","id":"cd00067","properties":[{"key":"EntryName","value":"GAL4"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"4.10.240.10","properties":[{"key":"EntryName","value":"Zn(2)-C6 fungal-type DNA-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR021858","properties":[{"key":"EntryName","value":"Fun_TF"}]},{"database":"InterPro","id":"IPR036864","properties":[{"key":"EntryName","value":"Zn2-C6_fun-type_DNA-bd_sf"}]},{"database":"InterPro","id":"IPR001138","properties":[{"key":"EntryName","value":"Zn2Cys6_DnaBD"}]},{"database":"PANTHER","id":"PTHR37534","properties":[{"key":"EntryName","value":"TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR37534:SF7","properties":[{"key":"EntryName","value":"TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11951","properties":[{"key":"EntryName","value":"Fungal_trans_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00172","properties":[{"key":"EntryName","value":"Zn_clus"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00066","properties":[{"key":"EntryName","value":"GAL4"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF57701","properties":[{"key":"EntryName","value":"Zn2/Cys6 DNA-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00463","properties":[{"key":"EntryName","value":"ZN2_CY6_FUNGAL_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50048","properties":[{"key":"EntryName","value":"ZN2_CY6_FUNGAL_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNYGVEKLKLKYSKHGCITCKIRKKRCSEDKPVCRDCRRLSFPCIYISESVDKQSLKKIKADIQHQLISKKRKHAPDSAQKAAVATRTRRVGSDEQDNQVYLSKPLEDCISQKLDSMGLQLYNYYRSHLANIISIAPMNQNYYLNIFLPMAHENDGILFAILAWSANHLSISSSNELRKDEIFVNLANKYTYMSLSHLKTNEGSSACAKLGFLYSLAQILILCGSEICQGDVKFWKILLNIGKNLIENHVGKDVSRILTTTTEEPSLEERIIFPNFNSVVKYWLIVNFIYHDILNFNTTSFPIEQYEKFFQRDQNSLPSSANFIESIDSPIEEIDPLIGINKPILLLLGQVTNLTRFLQTMEQEEMLEHGDKILSLQVEIYKLQPSLMALEHLDDEKKFYYLELFEIMKISTLMFFQLTLLKIDKDSLELQILRNKLDSKLDKVIGTFLEGSLCFPLFIYGVCIQVEDMEKKIDLEAKFDDILKRYKCYNFQNARLLIRKIWQNEADGISEHDLVHMIDELDYNINFA","length":528,"molWeight":61224,"crc64":"DFA04DDE29937942","md5":"02EA14A22623673A1FEE8A3B22268D16"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1},"countByFeatureType":{"Chain":1,"DNA binding":1,"Motif":1,"Mutagenesis":2},"uniParcId":"UPI0000137AE8"}}