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SAGA acts as a general cofactor required for essentially all RNA polymerase II transcription (PubMed:10026213, PubMed:10864329, PubMed:15647753, PubMed:25216679, PubMed:28918903). At the promoters, SAGA is required for transcription pre-initiation complex (PIC) recruitment. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (via core/TAF module) and promoter selectivity, interaction with transcription activators (via Tra1/SPT module), and chromatin modification through histone acetylation (via HAT module) and deubiquitination (via DUB module) (PubMed:22224423). SAGA preferentially acetylates histones H3 (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac) and H2B and deubiquitinates histone H2B (PubMed:10026213). SAGA interacts with DNA via upstream activating sequences (UASs) (PubMed:28918903). Also identified in a modified version of SAGA named SALSA or SLIK (PubMed:15647753). The cleavage of SPT7 and the absence of the SPT8 subunit in SLIK neither drive any major conformational differences in its structure compared with SAGA, nor significantly affect HAT, DUB, or DNA-binding activities (PubMed:33864814). SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PubMed:21685874). Component of the ADA histone acetyltransferase complex, which preferentially acetylates nucleosomal histones H3 (to form H3K14ac and H3K18ac) and H2B (PubMed:10026213). SGF29 is also required for heterochromatin boundary formation function, prevention of the spread of gene silencing at the HMR locus (PubMed:24307402)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12052880"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15647753"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21685874"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21734642"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22224423"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25216679"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33864814"}],"value":"Component of the 1.8 MDa SAGA (Spt-Ada-Gcn5 acetyltransferase) complex, which is composed of 19 subunits TRA1, SPT7, TAF5, NGG1/ADA3, SGF73, SPT20/ADA5, SPT8, TAF12, TAF6, HFI1/ADA1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10, TAF9, SGF11 and SUS1 (PubMed:12052880). The SAGA complex is composed of 4 modules, namely the HAT (histone acetyltransferase) module (GCN5, ADA2, NGG1/ADA3 and SGF29), the DUB (deubiquitinating) module (UBP8, SGF11, SGF73 and SUS1), the core or TAF (TBP-associated factor) module (TAF5, TAF6, TAF9, TAF10 and TAF12), and the Tra1 or SPT (Suppressor of Ty) module (TRA1, HFI1/ADA1, SPT3, SPT7, SPT8 and SPT20/ADA5). The Tra1/SPT module binds activators, the core module recruits TBP (TATA-binding protein), the HAT module contains the histone H3 acetyltransferase GCN5, and the DUB module comprises the histone H2B deubiquitinase UBP8 (PubMed:25216679). Also identified in an altered form of SAGA, named SALSA (SAGA altered, Spt8 absent) or SLIK (SAGA-like) complex, which contains a C-terminal truncated form of SPT7 and is missing SPT8 (PubMed:15647753). However, it has been shown that the SAGA and SAGA-like SALSA/SLIK transcriptional coactivators are structurally and biochemically equivalent (PubMed:33864814). Interacts with dimethylated and trimethylated 'Lys-4' of histone H3 (H3K4me2 and H3K4me3), with a preference for the trimethylated form (H3K4me3) (PubMed:21685874). Component of the 0.8 MDa ADA complex, a HAT complex distinct from SAGA, which at least consists of ADA2, NGG1/ADA3, AHC1, AHC2, SGF29 and GCN5 (PubMed:21734642, PubMed:22224423)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P25554","intActId":"EBI-21678"},"interactantTwo":{"uniProtKBAccession":"Q12060","geneName":"HFI1","intActId":"EBI-8287"},"numberOfExperiments":10,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P25554","intActId":"EBI-21678"},"interactantTwo":{"uniProtKBAccession":"P32494","geneName":"NGG1","intActId":"EBI-2192"},"numberOfExperiments":6,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P25554","intActId":"EBI-21678"},"interactantTwo":{"uniProtKBAccession":"P68431","geneName":"H3C12","intActId":"EBI-79722"},"numberOfExperiments":11,"organismDiffer":true}]},{"commentType":"SUBCELLULAR 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