{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P23883","secondaryAccessions":["P78250","Q5H774"],"uniProtkbId":"PUUC_ECOLI","entryAudit":{"firstPublicDate":"1991-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-11-01","entryVersion":169,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15590624"}],"value":"Gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15590624"}],"value":"Gamma-Glu-gamma-aminobutyraldehyde dehydrogenase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15590624"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"22636776"}],"value":"1.2.1.99"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"1840553"}],"value":"Aldehyde dehydrogenase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"1840553"}],"value":"ALDH"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22636776"}],"value":"Aminobutyraldehyde dehydrogenase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22636776"}],"value":"ABDH"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22636776"}],"value":"1.2.1.19"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22636776"}],"value":"Succinate-semialdehyde dehydrogenase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22636776"}],"value":"SSDH"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22636776"}],"value":"1.2.1.24"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15590624"}],"value":"puuC"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"1840553"}],"value":"aldH"}],"orderedLocusNames":[{"value":"b1300"},{"value":"JW1293"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15590624"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22636776"}],"value":"Dehydrogenase involved in the degradation of putrescine (PubMed:15590624, PubMed:22636776). Acts as a non-specific aldehyde dehydrogenase that can catalyze three separate reactions in putrescine catabolism: the oxidation of gamma-glutamyl-gamma-aminobutyraldehyde to gamma-glutamyl-gamma-aminobutyrate (gamma-Glu-GABA), the oxidation 4-aminobutanal (gamma-aminobutyraldehyde) to 4-aminobutanoate (gamma-aminobutyrate or GABA) and the oxidation of succinate semialdehyde to succinate (PubMed:15590624, PubMed:22636776). In vitro, it exhibits broad substrate specificity for various aliphatic and aromatic aldehydes (PubMed:18668238). It can use both NAD(+) and NADP(+), but NAD(+) is significantly preferred over NADP(+) for many aldehydes (PubMed:18668238). Among the aldehydes tested, 3-hydroxypropanal (3-hydroxypropionaldehyde or 3-HPA) is the best substrate, followed by 3-methylbutanal (isovaleraldehyde), propanal (propionaldehyde), butanal (butyraldehyde), pentanal (valeraldehyde), benzaldehyde, furfural (2-furaldehyde) and acetaldehyde (PubMed:18668238). The enzyme cannot use formaldehyde (PubMed:18668238). It might play a role in propionate and/or acetic acid metabolisms in addition to its function in putrescine metabolism (PubMed:18668238)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"4-(gamma-L-glutamylamino)butanal + NAD(+) + H2O = 4-(gamma-L-glutamylamino)butanoate + NADH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42340"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:58800"},{"database":"ChEBI","id":"CHEBI:61508"}],"ecNumber":"1.2.1.99","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15590624"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"22636776"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:42341"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15590624"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"22636776"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"4-(gamma-L-glutamylamino)butanal + NADP(+) + H2O = 4-(gamma-L-glutamylamino)butanoate + NADPH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:28410"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:58800"},{"database":"ChEBI","id":"CHEBI:61508"}],"ecNumber":"1.2.1.99","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15590624"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:28411"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15590624"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"4-aminobutanal + NAD(+) + H2O = 4-aminobutanoate + NADH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:19105"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:58264"},{"database":"ChEBI","id":"CHEBI:59888"}],"ecNumber":"1.2.1.19","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22636776"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:19106"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22636776"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"succinate semialdehyde + NAD(+) + H2O = succinate + NADH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:13217"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30031"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57706"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"1.2.1.24","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22636776"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:13218"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22636776"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-hydroxypropanal + NAD(+) + H2O = 3-hydroxypropanoate + NADH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:59432"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16510"},{"database":"ChEBI","id":"CHEBI:17871"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-hydroxypropanal + NADP(+) + H2O = 3-hydroxypropanoate + NADPH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:59428"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16510"},{"database":"ChEBI","id":"CHEBI:17871"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-methylbutanal + NAD(+) + H2O = 3-methylbutanoate + NADH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:76655"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16638"},{"database":"ChEBI","id":"CHEBI:48942"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-methylbutanal + NADP(+) + H2O = 3-methylbutanoate + NADPH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:76651"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16638"},{"database":"ChEBI","id":"CHEBI:48942"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"propanal + NAD(+) + H2O = propanoate + NADH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:67256"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17153"},{"database":"ChEBI","id":"CHEBI:17272"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"propanal + NADP(+) + H2O = propanoate + NADPH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:27918"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17153"},{"database":"ChEBI","id":"CHEBI:17272"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"butanal + NAD(+) + H2O = butanoate + NADH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:69088"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15743"},{"database":"ChEBI","id":"CHEBI:17968"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"butanal + NADP(+) + H2O = butanoate + NADPH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:76659"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15743"},{"database":"ChEBI","id":"CHEBI:17968"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"pentanal + NAD(+) + H2O = pentanoate + NADH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:69092"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:31011"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:84069"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"pentanal + NADP(+) + H2O = pentanoate + NADPH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:76663"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:31011"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:84069"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"benzaldehyde + NAD(+) + H2O = benzoate + NADH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11840"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16150"},{"database":"ChEBI","id":"CHEBI:17169"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"benzaldehyde + NADP(+) + H2O = benzoate + NADPH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21660"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16150"},{"database":"ChEBI","id":"CHEBI:17169"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"furfural + NAD(+) + H2O = 2-furoate + NADH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:76667"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16739"},{"database":"ChEBI","id":"CHEBI:34768"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"furfural + NADP(+) + H2O = 2-furoate + NADPH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:76671"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16739"},{"database":"ChEBI","id":"CHEBI:34768"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"acetaldehyde + NAD(+) + H2O = acetate + NADH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25294"},{"database":"ChEBI","id":"CHEBI:15343"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30089"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"1840553"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"acetaldehyde + NADP(+) + H2O = acetate + NADPH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25298"},{"database":"ChEBI","id":"CHEBI:15343"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30089"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"1840553"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}],"value":"Activity is enhanced in the presence of disulfide reductants such as dithiothreitol (DTT) or 2-mercaptoethanol (PubMed:18668238). Activity is completely inhibited in the presence of heavy metals (mercury, silver) or sodium hydrogensulfite, and strongly inhibited in the presence of lithium, copper and zinc ions (PubMed:18668238). Slightly inhibited by potassium, sodium and ammonium ions (PubMed:18668238)"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":32.1,"unit":"umol/min/mg","enzyme":"enzyme with 3-hydroxypropanal as substrate (with NAD at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"velocity":5.5,"unit":"umol/min/mg","enzyme":"enzyme with 3-hydroxypropanal as substrate (with NADP at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"velocity":30.67,"unit":"umol/min/mg","enzyme":"enzyme with 3-methylbutanal as substrate (with NAD at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"velocity":27.35,"unit":"umol/min/mg","enzyme":"enzyme with propanal as substrate (with NAD at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"velocity":30.07,"unit":"umol/min/mg","enzyme":"enzyme with butanal as substrate (with NAD at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"velocity":29.47,"unit":"umol/min/mg","enzyme":"enzyme with pentanal as substrate (with NAD at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"velocity":19.51,"unit":"umol/min/mg","enzyme":"enzyme with benzaldehyde as substrate (with NADP at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"velocity":12.39,"unit":"umol/min/mg","enzyme":"enzyme with acetaldehyde as substrate (with NAD at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"velocity":0.3,"unit":"umol/min/mg","enzyme":"enzyme with 3-hydroxypropanoate as substrate (with NADH at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"velocity":30.1,"unit":"umol/min/mg","enzyme":"enzyme with NAD as substrate (with 3-hydroxypropanal at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}],"michaelisConstants":[{"constant":0.49,"unit":"mM","substrate":"3-hydroxypropanal (with NAD at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"constant":0.29,"unit":"mM","substrate":"3-hydroxypropanal (with NADP at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"constant":0.68,"unit":"mM","substrate":"3-methylbutanal (with NAD at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"constant":1.21,"unit":"mM","substrate":"propanal (with NAD at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"constant":0.97,"unit":"mM","substrate":"butanal (with NAD at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"constant":0.24,"unit":"mM","substrate":"pentanal (with NAD at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"constant":5.37,"unit":"mM","substrate":"benzaldehyde (with NADP at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"constant":1.0,"unit":"mM","substrate":"acetaldehyde (with NAD at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"constant":0.12,"unit":"mM","substrate":"3-hydroxypropanoate (with NADH at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]},{"constant":0.06,"unit":"mM","substrate":"NAD (with 3-hydroxypropanal at pH 8 and at 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}],"value":"kcat is 28.54 sec(-1) with 3-hydroxypropanal as substrate (in the presence of NAD) (PubMed:18668238). kcat is 4.91 sec(-1) with 3-hydroxypropanal as substrate (in the presence of NADP) (PubMed:18668238). kcat is 27.31 sec(-1) with 3-methylbutanal as substrate (in the presence of NAD) (PubMed:18668238). kcat is 24.35 sec(-1) with propanal as substrate (in the presence of NAD) (PubMed:18668238). kcat is 26.95 sec(-1) with butanal as substrate (in the presence of NAD) (PubMed:18668238). kcat is 26.24 sec(-1) with pentanal as substrate (in the presence of NAD) (PubMed:18668238). kcat is 17.37 sec(-1) with benzaldehyde as substrate (in the presence of NADP) (PubMed:18668238). kcat is 11.03 sec(-1) with acetaldehyde as substrate (in the presence of NAD) (PubMed:18668238). kcat is 0.26 sec(-1) with 3-hydroxypropanoate as substrate (in the presence of NADH) (PubMed:18668238). kcat is 26.79 sec(-1) with NAD as substrate (in the presence of 3-hydroxypropanal) (PubMed:18668238)."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}],"value":"Optimum pH is 8 (PubMed:18668238). Activity is highly sensitive to pH variation, and an increase or decrease of one pH unit from the optimal attenuates more than 70% of activity (PubMed:18668238). At pH 6.0, retains only 6% of the activity (PubMed:18668238). At pH.5.0, the activity is completely abolished (PubMed:18668238)."}]},"temperatureDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18668238"}],"value":"Optimum temperature is 37 degrees Celsius (PubMed:18668238). 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