ID MIME_HUMAN Reviewed; 298 AA. AC P20774; Q6FIB0; Q9UF90; Q9UNK5; DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1991, sequence version 1. DT 02-OCT-2024, entry version 214. DE RecName: Full=Mimecan; DE AltName: Full=Osteoglycin; DE AltName: Full=Osteoinductive factor; DE Short=OIF; DE Flags: Precursor; GN Name=OGN; Synonyms=OIF, SLRR3A; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=2372374; DOI=10.1089/dna.1990.9.303; RA Madisen L., Neubauer M., Plowman G., Rosen D.M., Segarini P.R., Dasch J.R., RA Thompson A.Y., Ziman J., Bentz H., Purchio A.F.; RT "Molecular cloning of a novel bone-forming compound: osteoinductive RT factor."; RL DNA Cell Biol. 9:303-309(1990). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RA Grover J., Rodriguez E., Roughley P.J.; RL Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=10802664; DOI=10.1038/75664; RA Pellegata N.S., Dieguez-Lucena J.L., Joensuu T., Lau S., Montgomery K.T., RA Krahe R., Kivelae T., Kucherlapati R., Forsius H., de la Chapelle A.; RT "Mutations in KERA, encoding keratocan, cause cornea plana."; RL Nat. Genet. 25:91-95(2000). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Pituitary; RX PubMed=10931946; DOI=10.1073/pnas.160270997; RA Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., RA Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., RA Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M., Zhou J., Xu S.-H., Gu J., RA Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., RA Chen M.-D., Chen J.-L.; RT "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis RT and full-length cDNA cloning."; RL Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Uterus; RX PubMed=11230166; DOI=10.1101/gr.gr1547r; RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B., RA Klein M., Poustka A.; RT "Towards a catalog of human genes and proteins: sequencing and analysis of RT 500 novel complete protein coding human cDNAs."; RL Genome Res. 11:422-435(2001). RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.; RT "Cloning of human full open reading frames in Gateway(TM) system entry RT vector (pDONR201)."; RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. RN [7] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=15164053; DOI=10.1038/nature02465; RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., RA Dunham I.; RT "DNA sequence and analysis of human chromosome 9."; RL Nature 429:369-374(2004). RN [8] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Testis; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [9] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-54. RX PubMed=10373482; DOI=10.1074/jbc.274.26.18693; RA Tasheva E.S., Funderburgh M.L., McReynolds J., Funderburgh J.L., RA Conrad G.W.; RT "The bovine mimecan gene. Molecular cloning and characterization of two RT major RNA transcripts generated by alternative use of two splice acceptor RT sites in the third exon."; RL J. Biol. Chem. 274:18693-18701(1999). RN [10] RP PROTEIN SEQUENCE OF 21-35. RX PubMed=15340161; DOI=10.1110/ps.04682504; RA Zhang Z., Henzel W.J.; RT "Signal peptide prediction based on analysis of experimentally verified RT cleavage sites."; RL Protein Sci. 13:2819-2824(2004). RN [11] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 156-298. RC TISSUE=Uterus; RX PubMed=17974005; DOI=10.1186/1471-2164-8-399; RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., RA Wiemann S., Schupp I.; RT "The full-ORF clone resource of the German cDNA consortium."; RL BMC Genomics 8:399-399(2007). RN [12] RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-258. RC TISSUE=Liver; RX PubMed=19159218; DOI=10.1021/pr8008012; RA Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.; RT "Glycoproteomics analysis of human liver tissue by combination of multiple RT enzyme digestion and hydrazide chemistry."; RL J. Proteome Res. 8:651-661(2009). RN [13] RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-80, AND STRUCTURE OF RP CARBOHYDRATES. RC TISSUE=Cerebrospinal fluid; RX PubMed=19838169; DOI=10.1038/nmeth.1392; RA Nilsson J., Rueetschi U., Halim A., Hesse C., Carlsohn E., Brinkmalm G., RA Larson G.; RT "Enrichment of glycopeptides for glycan structure and attachment site RT identification."; RL Nat. Methods 6:809-811(2009). RN [14] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Liver; RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014; RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., RA Ye M., Zou H.; RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver RT phosphoproteome."; RL J. Proteomics 96:253-262(2014). CC -!- FUNCTION: Induces bone formation in conjunction with TGF-beta-1 or TGF- CC beta-2. {ECO:0000250|UniProtKB:P19879}. CC -!- INTERACTION: CC P20774; P46108: CRK; NbExp=2; IntAct=EBI-1753690, EBI-886; CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular CC matrix {ECO:0000250|UniProtKB:Q8MJF1}. CC -!- TISSUE SPECIFICITY: Bone. CC -!- PTM: O-glycosylated with a core 1 or possibly core 8 glycan. CC {ECO:0000269|PubMed:19838169}. CC -!- PTM: Contains keratan sulfate. {ECO:0000250|UniProtKB:P19879}. CC -!- SIMILARITY: Belongs to the small leucine-rich proteoglycan (SLRP) CC family. SLRP class III subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF192483; AAF19364.1; -; Genomic_DNA. DR EMBL; AF192478; AAF19364.1; JOINED; Genomic_DNA. DR EMBL; AF192479; AAF19364.1; JOINED; Genomic_DNA. DR EMBL; AF192480; AAF19364.1; JOINED; Genomic_DNA. DR EMBL; AF192481; AAF19364.1; JOINED; Genomic_DNA. DR EMBL; AF192482; AAF19364.1; JOINED; Genomic_DNA. DR EMBL; AF202167; AAF69109.1; -; mRNA. DR EMBL; AF100758; AAD43022.1; -; mRNA. DR EMBL; AL110267; CAB53706.1; -; mRNA. DR EMBL; CR533516; CAG38547.1; -; mRNA. DR EMBL; AL137848; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; BC037273; AAH37273.1; -; mRNA. DR EMBL; BC095443; AAH95443.1; -; mRNA. DR EMBL; AF112465; AAD40453.1; -; Genomic_DNA. DR EMBL; AL133114; CAB61417.2; -; mRNA. DR CCDS; CCDS6695.1; -. DR PIR; B35272; B35272. DR RefSeq; NP_054776.1; NM_014057.4. DR RefSeq; NP_077727.3; NM_024416.4. DR RefSeq; NP_148935.1; NM_033014.3. DR AlphaFoldDB; P20774; -. DR SMR; P20774; -. DR BioGRID; 111019; 13. DR IntAct; P20774; 12. DR STRING; 9606.ENSP00000364711; -. DR GlyConnect; 767; 13 N-Linked glycans (1 site), 1 O-Linked glycan (1 site). DR GlyCosmos; P20774; 3 sites, 14 glycans. DR GlyGen; P20774; 4 sites, 12 N-linked glycans (1 site), 2 O-linked glycans (1 site). DR iPTMnet; P20774; -. DR PhosphoSitePlus; P20774; -. DR SwissPalm; P20774; -. DR BioMuta; OGN; -. DR DMDM; 129078; -. DR REPRODUCTION-2DPAGE; IPI00025465; -. DR jPOST; P20774; -. DR MassIVE; P20774; -. DR PaxDb; 9606-ENSP00000262551; -. DR PeptideAtlas; P20774; -. DR ProteomicsDB; 53784; -. DR Antibodypedia; 2122; 327 antibodies from 34 providers. DR DNASU; 4969; -. DR Ensembl; ENST00000262551.8; ENSP00000262551.4; ENSG00000106809.11. DR Ensembl; ENST00000375561.10; ENSP00000364711.5; ENSG00000106809.11. DR Ensembl; ENST00000707237.1; ENSP00000516801.1; ENSG00000291350.1. DR Ensembl; ENST00000707238.1; ENSP00000516802.1; ENSG00000291350.1. DR GeneID; 4969; -. DR KEGG; hsa:4969; -. DR MANE-Select; ENST00000375561.10; ENSP00000364711.5; NM_014057.5; NP_054776.1. DR UCSC; uc004asa.4; human. DR AGR; HGNC:8126; -. DR CTD; 4969; -. DR DisGeNET; 4969; -. DR GeneCards; OGN; -. DR HGNC; HGNC:8126; OGN. DR HPA; ENSG00000106809; Tissue enhanced (cervix, ovary). DR MIM; 602383; gene. DR neXtProt; NX_P20774; -. DR OpenTargets; ENSG00000106809; -. DR PharmGKB; PA31913; -. DR VEuPathDB; HostDB:ENSG00000106809; -. DR eggNOG; KOG0619; Eukaryota. DR GeneTree; ENSGT00940000157238; -. DR HOGENOM; CLU_067583_1_0_1; -. DR InParanoid; P20774; -. DR OMA; RIIHLQF; -. DR OrthoDB; 1080036at2759; -. DR PhylomeDB; P20774; -. DR TreeFam; TF351924; -. DR PathwayCommons; P20774; -. DR Reactome; R-HSA-2022854; Keratan sulfate biosynthesis. DR Reactome; R-HSA-2022857; Keratan sulfate degradation. DR Reactome; R-HSA-3656225; Defective CHST6 causes MCDC1. DR Reactome; R-HSA-3656243; Defective ST3GAL3 causes MCT12 and EIEE15. DR Reactome; R-HSA-3656244; Defective B4GALT1 causes B4GALT1-CDG (CDG-2d). DR SignaLink; P20774; -. DR BioGRID-ORCS; 4969; 10 hits in 1140 CRISPR screens. DR ChiTaRS; OGN; human. DR GeneWiki; OGN_(gene); -. DR GenomeRNAi; 4969; -. DR Pharos; P20774; Tbio. DR PRO; PR:P20774; -. DR Proteomes; UP000005640; Chromosome 9. DR RNAct; P20774; protein. DR Bgee; ENSG00000106809; Expressed in right coronary artery and 176 other cell types or tissues. DR ExpressionAtlas; P20774; baseline and differential. DR GO; GO:0062023; C:collagen-containing extracellular matrix; HDA:BHF-UCL. DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB. DR GO; GO:0031012; C:extracellular matrix; IBA:GO_Central. DR GO; GO:0005576; C:extracellular region; HDA:BHF-UCL. DR GO; GO:0005615; C:extracellular space; HDA:BHF-UCL. DR GO; GO:1903561; C:extracellular vesicle; HDA:UniProtKB. DR GO; GO:0005796; C:Golgi lumen; TAS:Reactome. DR GO; GO:0043202; C:lysosomal lumen; TAS:Reactome. DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW. DR GO; GO:0061975; P:articular cartilage development; IBA:GO_Central. DR GO; GO:0060348; P:bone development; IBA:GO_Central. DR GO; GO:0048662; P:negative regulation of smooth muscle cell proliferation; ISS:UniProtKB. DR Gene3D; 3.80.10.10; Ribonuclease Inhibitor; 1. DR InterPro; IPR001611; Leu-rich_rpt. DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp. DR InterPro; IPR032675; LRR_dom_sf. DR InterPro; IPR043547; Mimecan/Epiphycan/Opticin. DR PANTHER; PTHR46269; EPIPHYCAN-RELATED; 1. DR PANTHER; PTHR46269:SF1; MIMECAN; 1. DR Pfam; PF13855; LRR_8; 1. DR SMART; SM00369; LRR_TYP; 4. DR SUPFAM; SSF52058; L domain-like; 1. DR PROSITE; PS51450; LRR; 4. PE 1: Evidence at protein level; KW Direct protein sequencing; Disulfide bond; Extracellular matrix; KW Glycoprotein; Growth factor; Leucine-rich repeat; Proteoglycan; KW Proteomics identification; Reference proteome; Repeat; Secreted; Signal. FT SIGNAL 1..20 FT /evidence="ECO:0000269|PubMed:15340161" FT CHAIN 21..298 FT /note="Mimecan" FT /id="PRO_0000032760" FT REPEAT 112..131 FT /note="LRR 1" FT REPEAT 132..155 FT /note="LRR 2" FT REPEAT 156..179 FT /note="LRR 3" FT REPEAT 180..199 FT /note="LRR 4" FT REPEAT 200..225 FT /note="LRR 5" FT REPEAT 226..246 FT /note="LRR 6" FT REPEAT 247..277 FT /note="LRR 7" FT CARBOHYD 80 FT /note="O-linked (GalNAc...) threonine" FT /evidence="ECO:0000269|PubMed:19838169" FT CARBOHYD 88 FT /note="N-linked (GlcNAc...) (keratan sulfate) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 214 FT /note="N-linked (GlcNAc...) (keratan sulfate) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 258 FT /note="N-linked (GlcNAc...) (keratan sulfate) asparagine" FT /evidence="ECO:0000269|PubMed:19159218" FT DISULFID 255..288 FT /evidence="ECO:0000250|UniProtKB:P19879" SQ SEQUENCE 298 AA; 33922 MW; F4590785C3849F83 CRC64; MKTLQSTLLL LLLVPLIKPA PPTQQDSRII YDYGTDNFEE SIFSQDYEDK YLDGKNIKEK ETVIIPNEKS LQLQKDEAIT PLPPKKENDE MPTCLLCVCL SGSVYCEEVD IDAVPPLPKE SAYLYARFNK IKKLTAKDFA DIPNLRRLDF TGNLIEDIED GTFSKLSLLE ELSLAENQLL KLPVLPPKLT LFNAKYNKIK SRGIKANAFK KLNNLTFLYL DHNALESVPL NLPESLRVIH LQFNNIASIT DDTFCKANDT SYIRDRIEEI RLEGNPIVLG KHPNSFICLK RLPIGSYF //