{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P20061","secondaryAccessions":["A8KAC5","Q8WV77"],"uniProtkbId":"TCO1_HUMAN","entryAudit":{"firstPublicDate":"1991-02-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-05-01","entryVersion":204,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Transcobalamin-1"},"shortNames":[{"value":"TC-1"}]},"alternativeNames":[{"fullName":{"value":"Haptocorrin"},"shortNames":[{"value":"HC"}]},{"fullName":{"value":"Protein R"}},{"fullName":{"value":"Transcobalamin I"},"shortNames":[{"value":"TC I"},{"value":"TCI"}]}],"flag":"Precursor"},"genes":[{"geneName":{"value":"TCN1"},"synonyms":[{"value":"TC1"}]}],"comments":[{"texts":[{"value":"Binds vitamin B12 with femtomolar affinity and protects it from the acidic environment of the stomach"}],"commentType":"FUNCTION"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P20061","intActId":"EBI-2557232"},"interactantTwo":{"uniProtKBAccession":"Q8WXH2","geneName":"JPH3","intActId":"EBI-1055254"},"numberOfExperiments":3,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2777761"}],"value":"Produced by the salivary glands of the oral cavity, in response to ingestion of food. Major constituent of secondary granules in neutrophils"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"value":"Contains about 30% carbohydrates"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the eukaryotic cobalamin transport proteins family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":""},{"type":"Chain","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":433,"modifier":"EXACT"}},"description":"Transcobalamin-1","featureId":"PRO_0000005561"},{"type":"Region","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":310,"modifier":"EXACT"}},"description":"Globular N-terminal alpha domain"},{"type":"Region","location":{"start":{"value":311,"modifier":"EXACT"},"end":{"value":332,"modifier":"EXACT"}},"description":"Flexible linker"},{"type":"Region","location":{"start":{"value":333,"modifier":"EXACT"},"end":{"value":433,"modifier":"EXACT"}},"description":"Globular C-terminal beta domain"},{"type":"Binding site","location":{"start":{"value":142,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17439"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4KKI"}],"ligand":{"name":"cyanocob(III)alamin","id":"ChEBI:CHEBI:17439"}},{"type":"Binding site","location":{"start":{"value":186,"modifier":"EXACT"},"end":{"value":186,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17439"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4KKI"}],"ligand":{"name":"cyanocob(III)alamin","id":"ChEBI:CHEBI:17439"}},{"type":"Binding site","location":{"start":{"value":240,"modifier":"EXACT"},"end":{"value":240,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17439"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4KKI"}],"ligand":{"name":"cyanocob(III)alamin","id":"ChEBI:CHEBI:17439"}},{"type":"Binding site","location":{"start":{"value":289,"modifier":"EXACT"},"end":{"value":289,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17439"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4KKI"}],"ligand":{"name":"cyanocob(III)alamin","id":"ChEBI:CHEBI:17439"}},{"type":"Binding site","location":{"start":{"value":385,"modifier":"EXACT"},"end":{"value":386,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17439"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4KKI"}],"ligand":{"name":"cyanocob(III)alamin","id":"ChEBI:CHEBI:17439"}},{"type":"Binding site","location":{"start":{"value":402,"modifier":"EXACT"},"end":{"value":404,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17439"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4KKI"}],"ligand":{"name":"cyanocob(III)alamin","id":"ChEBI:CHEBI:17439"}},{"type":"Binding site","location":{"start":{"value":411,"modifier":"EXACT"},"end":{"value":411,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17439"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4KKI"}],"ligand":{"name":"cyanocob(III)alamin","id":"ChEBI:CHEBI:17439"}},{"type":"Binding site","location":{"start":{"value":433,"modifier":"EXACT"},"end":{"value":433,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17439"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4KKI"}],"ligand":{"name":"cyanocob(III)alamin","id":"ChEBI:CHEBI:17439"}},{"type":"Glycosylation","location":{"start":{"value":160,"modifier":"EXACT"},"end":{"value":160,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":216,"modifier":"EXACT"},"end":{"value":216,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16335952"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16740002"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":316,"modifier":"EXACT"},"end":{"value":316,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":337,"modifier":"EXACT"},"end":{"value":337,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":343,"modifier":"EXACT"},"end":{"value":343,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":349,"modifier":"EXACT"},"end":{"value":349,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":354,"modifier":"EXACT"},"end":{"value":354,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":369,"modifier":"EXACT"},"end":{"value":369,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16740002"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"}],"featureId":""},{"type":"Disulfide bond","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":265,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"}]},{"type":"Disulfide bond","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":308,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"}]},{"type":"Disulfide bond","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":197,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"}]},{"type":"Disulfide bond","location":{"start":{"value":388,"modifier":"EXACT"},"end":{"value":393,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"}]},{"type":"Natural variant","location":{"start":{"value":35,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"in dbSNP:rs34528912","featureCrossReferences":[{"database":"dbSNP","id":"rs34528912"}],"featureId":"VAR_031923","alternativeSequence":{"originalSequence":"R","alternativeSequences":["H"]}},{"type":"Natural variant","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"in dbSNP:rs34324219","featureCrossReferences":[{"database":"dbSNP","id":"rs34324219"}],"featureId":"VAR_031924","alternativeSequence":{"originalSequence":"D","alternativeSequences":["Y"]}},{"type":"Sequence conflict","location":{"start":{"value":119,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"in Ref. 1; AAA61058","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"I","alternativeSequences":["T"]}},{"type":"Helix","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":45,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Beta strand","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKJ"}]},{"type":"Helix","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Turn","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":92,"modifier":"EXACT"},"end":{"value":102,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":111,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":118,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Turn","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":144,"modifier":"EXACT"},"end":{"value":156,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":169,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":175,"modifier":"EXACT"},"end":{"value":177,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":185,"modifier":"EXACT"},"end":{"value":203,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":213,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Beta strand","location":{"start":{"value":238,"modifier":"EXACT"},"end":{"value":240,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Turn","location":{"start":{"value":241,"modifier":"EXACT"},"end":{"value":243,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":244,"modifier":"EXACT"},"end":{"value":252,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":255,"modifier":"EXACT"},"end":{"value":257,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Turn","location":{"start":{"value":260,"modifier":"EXACT"},"end":{"value":262,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":277,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Turn","location":{"start":{"value":278,"modifier":"EXACT"},"end":{"value":281,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":284,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":299,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Beta strand","location":{"start":{"value":333,"modifier":"EXACT"},"end":{"value":353,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":358,"modifier":"EXACT"},"end":{"value":368,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Helix","location":{"start":{"value":370,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Beta strand","location":{"start":{"value":375,"modifier":"EXACT"},"end":{"value":379,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Beta strand","location":{"start":{"value":381,"modifier":"EXACT"},"end":{"value":389,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Turn","location":{"start":{"value":396,"modifier":"EXACT"},"end":{"value":399,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Beta strand","location":{"start":{"value":400,"modifier":"EXACT"},"end":{"value":406,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Turn","location":{"start":{"value":415,"modifier":"EXACT"},"end":{"value":417,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]},{"type":"Beta strand","location":{"start":{"value":426,"modifier":"EXACT"},"end":{"value":432,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4KKI"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0170","category":"Ligand","name":"Cobalt"},{"id":"KW-0171","category":"Biological process","name":"Cobalt transport"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-0406","category":"Biological process","name":"Ion transport"},{"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"2777761","citationType":"journal article","authors":["Johnston J.","Bollekens J.","Allen R.H.","Berliner N."],"citationCrossReferences":[{"database":"PubMed","id":"2777761"},{"database":"DOI","id":"10.1016/s0021-9258(18)71539-1"}],"title":"Structure of the cDNA encoding transcobalamin I, a neutrophil granule protein.","publicationDate":"1989","journal":"J. Biol. Chem.","firstPage":"15754","lastPage":"15757","volume":"264"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","TISSUE SPECIFICITY"]},{"referenceNumber":2,"citation":{"id":"14702039","citationType":"journal article","authors":["Ota T.","Suzuki Y.","Nishikawa T.","Otsuki T.","Sugiyama T.","Irie R.","Wakamatsu A.","Hayashi K.","Sato H.","Nagai K.","Kimura K.","Makita H.","Sekine M.","Obayashi M.","Nishi T.","Shibahara T.","Tanaka T.","Ishii S.","Yamamoto J.","Saito K.","Kawai Y.","Isono Y.","Nakamura Y.","Nagahari K.","Murakami K.","Yasuda T.","Iwayanagi T.","Wagatsuma M.","Shiratori A.","Sudo H.","Hosoiri T.","Kaku Y.","Kodaira H.","Kondo H.","Sugawara M.","Takahashi M.","Kanda K.","Yokoi T.","Furuya T.","Kikkawa E.","Omura Y.","Abe K.","Kamihara K.","Katsuta N.","Sato K.","Tanikawa M.","Yamazaki M.","Ninomiya K.","Ishibashi T.","Yamashita H.","Murakawa K.","Fujimori K.","Tanai H.","Kimata M.","Watanabe M.","Hiraoka S.","Chiba Y.","Ishida S.","Ono Y.","Takiguchi S.","Watanabe S.","Yosida M.","Hotuta T.","Kusano J.","Kanehori K.","Takahashi-Fujii A.","Hara H.","Tanase T.-O.","Nomura Y.","Togiya S.","Komai F.","Hara R.","Takeuchi K.","Arita M.","Imose N.","Musashino K.","Yuuki H.","Oshima A.","Sasaki N.","Aotsuka S.","Yoshikawa Y.","Matsunawa H.","Ichihara T.","Shiohata N.","Sano S.","Moriya S.","Momiyama H.","Satoh N.","Takami S.","Terashima Y.","Suzuki O.","Nakagawa S.","Senoh A.","Mizoguchi H.","Goto Y.","Shimizu F.","Wakebe H.","Hishigaki H.","Watanabe T.","Sugiyama A.","Takemoto M.","Kawakami B.","Yamazaki M.","Watanabe K.","Kumagai A.","Itakura S.","Fukuzumi Y.","Fujimori Y.","Komiyama M.","Tashiro H.","Tanigami A.","Fujiwara T.","Ono T.","Yamada K.","Fujii Y.","Ozaki K.","Hirao M.","Ohmori Y.","Kawabata A.","Hikiji T.","Kobatake N.","Inagaki H.","Ikema Y.","Okamoto S.","Okitani R.","Kawakami T.","Noguchi S.","Itoh T.","Shigeta K.","Senba T.","Matsumura K.","Nakajima Y.","Mizuno T.","Morinaga M.","Sasaki M.","Togashi T.","Oyama M.","Hata H.","Watanabe M.","Komatsu T.","Mizushima-Sugano J.","Satoh T.","Shirai Y.","Takahashi Y.","Nakagawa K.","Okumura K.","Nagase T.","Nomura N.","Kikuchi H.","Masuho Y.","Yamashita R.","Nakai K.","Yada T.","Nakamura Y.","Ohara O.","Isogai T.","Sugano S."],"citationCrossReferences":[{"database":"PubMed","id":"14702039"},{"database":"DOI","id":"10.1038/ng1285"}],"title":"Complete sequencing and characterization of 21,243 full-length human cDNAs.","publicationDate":"2004","journal":"Nat. Genet.","firstPage":"40","lastPage":"45","volume":"36"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Trachea","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"16554811","citationType":"journal article","authors":["Taylor T.D.","Noguchi H.","Totoki Y.","Toyoda A.","Kuroki Y.","Dewar K.","Lloyd C.","Itoh T.","Takeda T.","Kim D.-W.","She X.","Barlow K.F.","Bloom T.","Bruford E.","Chang J.L.","Cuomo C.A.","Eichler E.","FitzGerald M.G.","Jaffe D.B.","LaButti K.","Nicol R.","Park H.-S.","Seaman C.","Sougnez C.","Yang X.","Zimmer A.R.","Zody M.C.","Birren B.W.","Nusbaum C.","Fujiyama A.","Hattori M.","Rogers J.","Lander E.S.","Sakaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16554811"},{"database":"DOI","id":"10.1038/nature04632"}],"title":"Human chromosome 11 DNA sequence and analysis including novel gene identification.","publicationDate":"2006","journal":"Nature","firstPage":"497","lastPage":"500","volume":"440"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":4,"citation":{"id":"CI-5GBDQ6B103N1E","citationType":"submission","authors":["Mural R.J.","Istrail S.","Sutton G.G.","Florea L.","Halpern A.L.","Mobarry C.M.","Lippert R.","Walenz B.","Shatkay H.","Dew I.","Miller J.R.","Flanigan M.J.","Edwards N.J.","Bolanos R.","Fasulo D.","Halldorsson B.V.","Hannenhalli S.","Turner R.","Yooseph S.","Lu F.","Nusskern D.R.","Shue B.C.","Zheng X.H.","Zhong F.","Delcher A.L.","Huson D.H.","Kravitz S.A.","Mouchard L.","Reinert K.","Remington K.A.","Clark A.G.","Waterman M.S.","Eichler E.E.","Adams M.D.","Hunkapiller M.W.","Myers E.W.","Venter J.C."],"publicationDate":"JUL-2005","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":5,"citation":{"id":"16335952","citationType":"journal article","authors":["Liu T.","Qian W.-J.","Gritsenko M.A.","Camp D.G. II","Monroe M.E.","Moore R.J.","Smith R.D."],"citationCrossReferences":[{"database":"PubMed","id":"16335952"},{"database":"DOI","id":"10.1021/pr0502065"}],"title":"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.","publicationDate":"2005","journal":"J. Proteome Res.","firstPage":"2070","lastPage":"2080","volume":"4"},"referencePositions":["GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-216"],"referenceComments":[{"value":"Plasma","type":"TISSUE"}]},{"referenceNumber":6,"citation":{"id":"16740002","citationType":"journal article","authors":["Ramachandran P.","Boontheung P.","Xie Y.","Sondej M.","Wong D.T.","Loo J.A."],"citationCrossReferences":[{"database":"PubMed","id":"16740002"},{"database":"DOI","id":"10.1021/pr050492k"}],"title":"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry.","publicationDate":"2006","journal":"J. Proteome Res.","firstPage":"1493","lastPage":"1503","volume":"5"},"referencePositions":["GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-216 AND ASN-369"],"referenceComments":[{"value":"Saliva","type":"TISSUE"}]},{"referenceNumber":7,"citation":{"id":"23846701","citationType":"journal article","authors":["Furger E.","Frei D.C.","Schibli R.","Fischer E.","Prota A.E."],"citationCrossReferences":[{"database":"PubMed","id":"23846701"},{"database":"DOI","id":"10.1074/jbc.m113.483271"}],"title":"Structural basis for universal corrinoid recognition by the cobalamin transport protein haptocorrin.","publicationDate":"2013","journal":"J. Biol. Chem.","firstPage":"25466","lastPage":"25476","volume":"288"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH CYANOCOBALAMIN","DISULFIDE BONDS","COBALAMIN-BINDING SITES","GLYCOSYLATION AT ASN-216; ASN-316; ASN-337; ASN-343; ASN-349; ASN-354 AND ASN-369"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"4KKI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4KKJ"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"J05068","properties":[{"key":"ProteinId","value":"AAA61058.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK292990","properties":[{"key":"ProteinId","value":"BAF85679.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AP002347","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CH471076","properties":[{"key":"ProteinId","value":"EAW73861.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"CCDS","id":"CCDS7978.1","properties":[{"key":"Description","value":"-"}]},{"database":"PIR","id":"A34227","properties":[{"key":"EntryName","value":"A34227"}]},{"database":"RefSeq","id":"NP_001053.2","properties":[{"key":"NucleotideSequenceId","value":"NM_001062.4"}]},{"database":"PDB","id":"4KKI","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.35 A"},{"key":"Chains","value":"A=1-433"}]},{"database":"PDB","id":"4KKJ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"A=1-433"}]},{"database":"PDBsum","id":"4KKI","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4KKJ","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"112807","properties":[{"key":"Interactions","value":"14"}]},{"database":"FunCoup","id":"P20061","properties":[{"key":"Number of interactors","value":"74"}]},{"database":"IntAct","id":"P20061","properties":[{"key":"Interactions","value":"10"}]},{"database":"NDEx","id":"IQUERY-CP-TCN1","properties":[{"key":"Evidences","value":"2 NDEx IQuery Curated Pathways"}]},{"database":"STRING","id":"9606.ENSP00000257264","properties":[{"key":"Description","value":"-"}]},{"database":"DrugBank","id":"DB00115","properties":[{"key":"GenericName","value":"Cyanocobalamin"}]},{"database":"DrugBank","id":"DB00200","properties":[{"key":"GenericName","value":"Hydroxocobalamin"}]},{"database":"DrugCentral","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"GlyConnect","id":"P20061","properties":[{"key":"glycosylation","value":"10 N-Linked glycans (2 sites)"}]},{"database":"GlyCosmos","id":"P20061","properties":[{"key":"glycosylation","value":"8 sites, 10 glycans"}]},{"database":"GlyGen","id":"P20061","properties":[{"key":"glycosylation","value":"9 sites, 11 N-linked glycans (3 sites)"}]},{"database":"iPTMnet","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"BioMuta","id":"TCN1","properties":[{"key":"Description","value":"-"}]},{"database":"DMDM","id":"146345530","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"9606-ENSP00000257264","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"PRIDE","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"53719","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"27835","properties":[{"key":"antibodies","value":"203 antibodies from 22 providers"}]},{"database":"DNASU","id":"6947","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENST00000257264.4","properties":[{"key":"ProteinId","value":"ENSP00000257264.3"},{"key":"GeneId","value":"ENSG00000134827.8"}]},{"database":"GeneID","id":"6947","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hsa:6947","properties":[{"key":"Description","value":"-"}]},{"database":"MANE-Select","id":"ENST00000257264.4","properties":[{"key":"ProteinId","value":"ENSP00000257264.3"},{"key":"RefSeqNucleotideId","value":"NM_001062.4"},{"key":"RefSeqProteinId","value":"NP_001053.2"}]},{"database":"UCSC","id":"uc001noj.3","properties":[{"key":"OrganismName","value":"human"}]},{"database":"AGR","id":"HGNC:11652","properties":[{"key":"Description","value":"-"}]},{"database":"ClinPGx","id":"PA36403","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"6947","properties":[{"key":"Description","value":"-"}]},{"database":"DisGeNET","id":"6947","properties":[{"key":"Description","value":"-"}]},{"database":"GeneCards","id":"TCN1","properties":[{"key":"Description","value":"-"}]},{"database":"HGNC","id":"HGNC:11652","properties":[{"key":"GeneName","value":"TCN1"}]},{"database":"HPA","id":"ENSG00000134827","properties":[{"key":"ExpressionPatterns","value":"Tissue enriched (salivary)"}]},{"database":"MalaCards","id":"TCN1","properties":[{"key":"Description","value":"-"}]},{"database":"MIM","id":"189905","properties":[{"key":"Type","value":"gene"}]},{"database":"OpenTargets","id":"ENSG00000134827","properties":[{"key":"Description","value":"-"}]},{"database":"Orphanet","id":"2967","properties":[{"key":"Disease","value":"Haptocorrin deficiency"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000134827","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502QT7B","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00530000063370","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_052188_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"TMNQSKY","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"6343110at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PAN-GO","id":"P20061","properties":[{"key":"Number of GO annotations","value":"3 GO annotations based on evolutionary models"}]},{"database":"PhylomeDB","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"PathwayCommons","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-HSA-6798695","properties":[{"key":"PathwayName","value":"Neutrophil degranulation"}]},{"database":"Reactome","id":"R-HSA-9758881","properties":[{"key":"PathwayName","value":"Uptake of dietary cobalamins into enterocytes"}]},{"database":"Reactome","id":"R-HSA-9758890","properties":[{"key":"PathwayName","value":"Transport of RCbl within the body"}]},{"database":"SignaLink","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"6947","properties":[{"key":"hits","value":"11 hits in 1160 CRISPR screens"}]},{"database":"ChiTaRS","id":"TCN1","properties":[{"key":"OrganismName","value":"human"}]},{"database":"EvolutionaryTrace","id":"P20061","properties":[{"key":"Description","value":"-"}]},{"database":"GeneWiki","id":"Haptocorrin","properties":[{"key":"Description","value":"-"}]},{"database":"GenomeRNAi","id":"6947","properties":[{"key":"Description","value":"-"}]},{"database":"Pharos","id":"P20061","properties":[{"key":"DevelopmentLevel","value":"Tbio"}]},{"database":"PRO","id":"PR:P20061","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 11"}]},{"database":"RNAct","id":"P20061","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSG00000134827","properties":[{"key":"ExpressionPatterns","value":"Expressed in pancreatic ductal cell and 125 other cell types or tissues"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"HDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"16502470"}]},{"database":"GO","id":"GO:0035580","properties":[{"key":"GoTerm","value":"C:specific granule lumen"},{"key":"GoEvidenceType","value":"TAS:Reactome"}]},{"database":"GO","id":"GO:1904724","properties":[{"key":"GoTerm","value":"C:tertiary granule lumen"},{"key":"GoEvidenceType","value":"TAS:Reactome"}]},{"database":"GO","id":"GO:0140355","properties":[{"key":"GoTerm","value":"F:cargo receptor ligand activity"},{"key":"GoEvidenceType","value":"EXP:Reactome"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22547309"}]},{"database":"GO","id":"GO:0031419","properties":[{"key":"GoTerm","value":"F:cobalamin binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0140313","properties":[{"key":"GoTerm","value":"F:molecular sequestering activity"},{"key":"GoEvidenceType","value":"EXP:Reactome"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846701"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27411955"}]},{"database":"GO","id":"GO:0015889","properties":[{"key":"GoTerm","value":"P:cobalamin transport"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"DisProt","id":"DP02797","properties":[{"key":"Description","value":"-"}]},{"database":"FunFam","id":"1.50.10.20:FF:000048","properties":[{"key":"EntryName","value":"Transcobalamin 1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.170.130.30:FF:000003","properties":[{"key":"EntryName","value":"Transcobalamin 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.170.130.30","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002157","properties":[{"key":"EntryName","value":"Cbl-bd_prot"}]},{"database":"InterPro","id":"IPR051588","properties":[{"key":"EntryName","value":"Cobalamin_Transport"}]},{"database":"InterPro","id":"IPR027954","properties":[{"key":"EntryName","value":"Transcobalamin-like_C"}]},{"database":"PANTHER","id":"PTHR10559:SF13","properties":[{"key":"EntryName","value":"TRANSCOBALAMIN-1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10559","properties":[{"key":"EntryName","value":"TRANSCOBALAMIN-1/GASTRIC INTRINSIC FACTOR"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01122","properties":[{"key":"EntryName","value":"Cobalamin_bind"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF14478","properties":[{"key":"EntryName","value":"DUF4430"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00468","properties":[{"key":"EntryName","value":"COBALAMIN_BINDING"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MRQSHQLPLVGLLLFSFIPSQLCEICEVSEENYIRLKPLLNTMIQSNYNRGTSAVNVVLSLKLVGIQIQTLMQKMIQQIKYNVKSRLSDVSSGELALIILALGVCRNAEENLIYDYHLIDKLENKFQAEIENMEAHNGTPLTNYYQLSLDVLALCLFNGNYSTAEVVNHFTPENKNYYFGSQFSVDTGAMAVLALTCVKKSLINGQIKADEGSLKNISIYTKSLVEKILSEKKENGLIGNTFSTGEAMQALFVSSDYYNENDWNCQQTLNTVLTEISQGAFSNPNAAAQVLPALMGKTFLDINKDSSCVSASGNFNISADEPITVTPPDSQSYISVNYSVRINETYFTNVTVLNGSVFLSVMEKAQKMNDTIFGFTMEERSWGPYITCIQGLCANNNDRTYWELLSGGEPLSQGAGSYVVRNGENLEVRWSKY","length":433,"molWeight":48207,"crc64":"CF1052BE3DBA929A","md5":"F7A920090EAA51DADE9600AB93347C44"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"PTM":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Region":3,"Binding site":8,"Glycosylation":8,"Disulfide bond":4,"Natural variant":2,"Sequence conflict":1,"Helix":21,"Beta strand":7,"Turn":7},"uniParcId":"UPI000013CF55"}}