{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P19624","uniProtkbId":"PDXA_ECOLI","entryAudit":{"firstPublicDate":"1991-02-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1991-02-01","entryVersion":197,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"}],"value":"4-hydroxythreonine-4-phosphate dehydrogenase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026039"}],"value":"1.1.1.262"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"}],"value":"4-(phosphohydroxy)-L-threonine dehydrogenase"}}]},"genes":[{"geneName":{"value":"pdxA"},"orderedLocusNames":[{"value":"b0052"},{"value":"JW0051"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026039"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}],"value":"Catalyzes the NAD(P)-dependent oxidation of 4-(phosphooxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphooxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"4-(phosphooxy)-L-threonine + NAD(+) = 3-amino-2-oxopropyl phosphate + CO2 + NADH","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:32275"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:57279"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:58452"}],"ecNumber":"1.1.1.262","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026039"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12896974"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}},{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12896974"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Co(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12896974"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:48828"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12896974"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}],"value":"Binds 1 divalent metal cation per subunit. Can use ions such as Zn(2+), Mg(2+) or Co(2+)."}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":2.8,"unit":"umol/min/mg","enzyme":"enzyme with 4-(phosphooxy)-L-threonine as substrate (at 0.1 M Tris-HCl and pH 7.5)","evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}]},{"velocity":0.66,"unit":"umol/min/mg","enzyme":"enzyme with 4-(phosphooxy)-L-threonine as substrate (at 0.1 M phosphate buffer and pH 7.5)","evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}]}],"michaelisConstants":[{"constant":85.0,"unit":"uM","substrate":"4-(phosphooxy)-L-threonine (at 0.1 M Tris-HCl and pH 7.5)","evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}]},{"constant":113.0,"unit":"uM","substrate":"4-(phosphooxy)-L-threonine (at 0.1 M phosphate buffer and pH 7.5)","evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"}]}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"}],"value":"Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12896974"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P19624","intActId":"EBI-1112869"},"interactantTwo":{"uniProtKBAccession":"P19624","geneName":"pdxA","intActId":"EBI-1112869"},"numberOfExperiments":3,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12896974"}],"value":"The active site is located at the dimer interface"}],"commentType":"MISCELLANEOUS"},{"texts":[{"value":"According to PubMed:15026039, PdxA may catalyze both oxidation and decarboxylation reactions that directly lead to AHAP"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the PdxA family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":329,"modifier":"EXACT"}},"description":"4-hydroxythreonine-4-phosphate dehydrogenase","featureId":"PRO_0000188804"},{"type":"Binding site","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":136,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12896974"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":137,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12896974"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60240"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12896974"}],"ligand":{"name":"a divalent metal cation","id":"ChEBI:CHEBI:60240","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60240"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12896974"}],"ligand":{"name":"a divalent metal cation","id":"ChEBI:CHEBI:60240","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":266,"modifier":"EXACT"},"end":{"value":266,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60240"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12896974"}],"ligand":{"name":"a divalent metal cation","id":"ChEBI:CHEBI:60240","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":274,"modifier":"EXACT"},"end":{"value":274,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12896974"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":283,"modifier":"EXACT"},"end":{"value":283,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12896974"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":292,"modifier":"EXACT"},"end":{"value":292,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00536"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12896974"}],"ligand":{"name":"substrate"}},{"type":"Helix","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":5,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PS7"}]},{"type":"Beta strand","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":10,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":18,"modifier":"EXACT"},"end":{"value":25,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Beta strand","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":40,"modifier":"EXACT"},"end":{"value":49,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Beta strand","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":67,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Beta strand","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":93,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Beta strand","location":{"start":{"value":114,"modifier":"EXACT"},"end":{"value":118,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":123,"modifier":"EXACT"},"end":{"value":128,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Beta strand","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":157,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Beta strand","location":{"start":{"value":159,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":169,"modifier":"EXACT"},"end":{"value":171,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":172,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":178,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Beta strand","location":{"start":{"value":202,"modifier":"EXACT"},"end":{"value":206,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":210,"modifier":"EXACT"},"end":{"value":216,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":222,"modifier":"EXACT"},"end":{"value":226,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":227,"modifier":"EXACT"},"end":{"value":235,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Beta strand","location":{"start":{"value":239,"modifier":"EXACT"},"end":{"value":244,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":246,"modifier":"EXACT"},"end":{"value":249,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":255,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Beta strand","location":{"start":{"value":259,"modifier":"EXACT"},"end":{"value":265,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":266,"modifier":"EXACT"},"end":{"value":274,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Beta strand","location":{"start":{"value":278,"modifier":"EXACT"},"end":{"value":280,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Beta strand","location":{"start":{"value":282,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Helix","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":303,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]},{"type":"Turn","location":{"start":{"value":304,"modifier":"EXACT"},"end":{"value":306,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PS7"}]},{"type":"Helix","location":{"start":{"value":312,"modifier":"EXACT"},"end":{"value":328,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1PTM"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0170","category":"Ligand","name":"Cobalt"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0520","category":"Ligand","name":"NAD"},{"id":"KW-0521","category":"Ligand","name":"NADP"},{"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"id":"KW-0664","category":"Biological process","name":"Pyridoxine biosynthesis"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"2670894","citationType":"journal article","authors":["Roa B.B.","Connolly D.M.","Winkler M.E."],"citationCrossReferences":[{"database":"PubMed","id":"2670894"},{"database":"DOI","id":"10.1128/jb.171.9.4767-4777.1989"}],"title":"Overlap between pdxA and ksgA in the complex pdxA-ksgA-apaG-apaH operon of Escherichia coli K-12.","publicationDate":"1989","journal":"J. Bacteriol.","firstPage":"4767","lastPage":"4777","volume":"171"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"K12","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"1630901","citationType":"journal article","authors":["Yura T.","Mori H.","Nagai H.","Nagata T.","Ishihama A.","Fujita N.","Isono K.","Mizobuchi K.","Nakata A."],"citationCrossReferences":[{"database":"PubMed","id":"1630901"},{"database":"DOI","id":"10.1093/nar/20.13.3305"}],"title":"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region.","publicationDate":"1992","journal":"Nucleic Acids Res.","firstPage":"3305","lastPage":"3308","volume":"20"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-10D5JKK6I98A1","citationType":"journal article","authors":["Cane D.E.","Hsiung Y.","Cornish J.A.","Robinson J.K.","Spenser I.D."],"citationCrossReferences":[{"database":"DOI","id":"10.1021/ja9742085"}],"title":"Biosynthesis of vitamin B6: the oxidation of 4-hydroxy-L-threonine 4-phosphate by pdxA.","publicationDate":"1998","journal":"J. Am. Chem. Soc.","firstPage":"1936","lastPage":"1937","volume":"120"},"referencePositions":["FUNCTION","BIOPHYSICOCHEMICAL PROPERTIES","COFACTOR"]},{"referenceNumber":6,"citation":{"id":"15026039","citationType":"journal article","authors":["Banks J.","Cane D.E."],"citationCrossReferences":[{"database":"PubMed","id":"15026039"},{"database":"DOI","id":"10.1016/j.bmcl.2004.01.065"}],"title":"Biosynthesis of vitamin B6: direct identification of the product of the PdxA-catalyzed oxidation of 4-hydroxy-L-threonine-4-phosphate using electrospray ionization mass spectrometry.","publicationDate":"2004","journal":"Bioorg. Med. Chem. Lett.","firstPage":"1633","lastPage":"1636","volume":"14"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY"]},{"referenceNumber":7,"citation":{"id":"12896974","citationType":"journal article","authors":["Sivaraman J.","Li Y.","Banks J.","Cane D.E.","Matte A.","Cygler M."],"citationCrossReferences":[{"database":"PubMed","id":"12896974"},{"database":"DOI","id":"10.1074/jbc.m306344200"}],"title":"Crystal structure of Escherichia coli PdxA, an enzyme involved in the pyridoxal phosphate biosynthesis pathway.","publicationDate":"2003","journal":"J. Biol. Chem.","firstPage":"43682","lastPage":"43690","volume":"278"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS) IN COMPLEX WITH SUBSTRATE AND ZINC IONS","COFACTOR","SUBUNIT"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"M68521","properties":[{"key":"ProteinId","value":"AAA24305.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC73163.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAB96619.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"JV0026","properties":[{"key":"EntryName","value":"BVECXA"}]},{"database":"RefSeq","id":"NP_414594.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000241277.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"PDB","id":"1PS6","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.25 A"},{"key":"Chains","value":"A/B=2-329"}]},{"database":"PDB","id":"1PS7","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.47 A"},{"key":"Chains","value":"A/B/C/D=1-329"}]},{"database":"PDB","id":"1PTM","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.96 A"},{"key":"Chains","value":"A/B=1-329"}]},{"database":"PDBsum","id":"1PS6","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1PS7","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1PTM","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P19624","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P19624","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4261013","properties":[{"key":"Interactions","value":"9"}]},{"database":"BioGRID","id":"849317","properties":[{"key":"Interactions","value":"5"}]},{"database":"DIP","id":"DIP-10448N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P19624","properties":[{"key":"Number of interactors","value":"409"}]},{"database":"IntAct","id":"P19624","properties":[{"key":"Interactions","value":"5"}]},{"database":"STRING","id":"511145.b0052","properties":[{"key":"Description","value":"-"}]},{"database":"DrugBank","id":"DB02609","properties":[{"key":"GenericName","value":"4-Hydroxy-L-Threonine-5-Monophosphate"}]},{"database":"jPOST","id":"P19624","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b0052","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC73163","properties":[{"key":"ProteinId","value":"AAC73163"},{"key":"GeneId","value":"b0052"}]},{"database":"GeneID","id":"944919","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW0051","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b0052","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_00270","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.2231","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB0685","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG1995","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_040168_1_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P19624","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"FDIAYQN","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9801783at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P19624","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:PDXA-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:PDXA-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"1.1.1.262","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"UniPathway","id":"UPA00244","properties":[{"key":"RectionId","value":"UER00312"}]},{"database":"EvolutionaryTrace","id":"P19624","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P19624","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P19624","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0050570","properties":[{"key":"GoTerm","value":"F:4-hydroxythreonine-4-phosphate dehydrogenase activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15026039"}]},{"database":"GO","id":"GO:0050897","properties":[{"key":"GoTerm","value":"F:cobalt ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0042802","properties":[{"key":"GoTerm","value":"F:identical protein binding"},{"key":"GoEvidenceType","value":"IPI:IntAct"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"24561554"},{"evidenceCode":"ECO:0000353","source":"PubMed","id":"24627523"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0051287","properties":[{"key":"GoTerm","value":"F:NAD binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0042803","properties":[{"key":"GoTerm","value":"F:protein homodimerization activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12896974"}]},{"database":"GO","id":"GO:0008270","properties":[{"key":"GoTerm","value":"F:zinc ion binding"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12896974"}]},{"database":"GO","id":"GO:0042823","properties":[{"key":"GoTerm","value":"P:pyridoxal 5'-phosphate biosynthetic process"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"1537800"},{"evidenceCode":"ECO:0000315","source":"PubMed","id":"2670894"}]},{"database":"GO","id":"GO:0008615","properties":[{"key":"GoTerm","value":"P:pyridoxine biosynthetic process"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"1537800"},{"evidenceCode":"ECO:0000315","source":"PubMed","id":"2670894"}]},{"database":"FunFam","id":"3.40.718.10:FF:000010","properties":[{"key":"EntryName","value":"4-hydroxythreonine-4-phosphate dehydrogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.718.10","properties":[{"key":"EntryName","value":"Isopropylmalate Dehydrogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00536","properties":[{"key":"EntryName","value":"PdxA"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR037510","properties":[{"key":"EntryName","value":"PdxA"}]},{"database":"InterPro","id":"IPR005255","properties":[{"key":"EntryName","value":"PdxA_fam"}]},{"database":"NCBIfam","id":"TIGR00557","properties":[{"key":"EntryName","value":"pdxA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30004","properties":[{"key":"EntryName","value":"4-HYDROXYTHREONINE-4-PHOSPHATE DEHYDROGENASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30004:SF5","properties":[{"key":"EntryName","value":"4-HYDROXYTHREONINE-4-PHOSPHATE DEHYDROGENASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04166","properties":[{"key":"EntryName","value":"PdxA"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53659","properties":[{"key":"EntryName","value":"Isocitrate/Isopropylmalate dehydrogenase-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLTNRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRAPVTAGQLAVENGHYVVETLARACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRVALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHMGTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQGFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ","length":329,"molWeight":35114,"crc64":"44E1FEE233C86233","md5":"1CFADFA6EE50881AF75C0C606A5C991A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBUNIT":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":2,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":8,"Helix":18,"Beta strand":12,"Turn":1},"uniParcId":"UPI00001314F5"}}