{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P14540","secondaryAccessions":["D6VXM7"],"uniProtkbId":"ALF_YEAST","entryAudit":{"firstPublicDate":"1990-01-01","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2007-01-23","entryVersion":224,"sequenceVersion":3},"annotationScore":5.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Fructose-bisphosphate aldolase"},"shortNames":[{"value":"FBP aldolase"},{"value":"FBPA"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2647491"}],"value":"4.1.2.13"}]},"alternativeNames":[{"fullName":{"value":"Fructose-1,6-bisphosphate aldolase"}}]},"genes":[{"geneName":{"value":"FBA1"},"orderedLocusNames":[{"value":"YKL060C"}],"orfNames":[{"value":"YKL320"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:14729"},{"database":"ChEBI","id":"CHEBI:32966"},{"database":"ChEBI","id":"CHEBI:57642"},{"database":"ChEBI","id":"CHEBI:59776"}],"ecNumber":"4.1.2.13","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2647491"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution."}]}},{"texts":[{"value":"Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4"}],"commentType":"PATHWAY"},{"texts":[{"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562106"}],"value":"Present with 1020000 molecules/cell in log phase SD medium"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the class II fructose-bisphosphate aldolase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8935650"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":359,"modifier":"EXACT"}},"description":"Fructose-bisphosphate aldolase","featureId":"PRO_0000178762"},{"type":"Active site","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Binding site","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":111,"modifier":"EXACT"},"end":{"value":111,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":145,"modifier":"EXACT"},"end":{"value":145,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":175,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":227,"modifier":"EXACT"},"end":{"value":227,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":228,"modifier":"EXACT"},"end":{"value":228,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57642"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"dihydroxyacetone phosphate","id":"ChEBI:CHEBI:57642"}},{"type":"Binding site","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":265,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":266,"modifier":"EXACT"},"end":{"value":268,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57642"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"dihydroxyacetone phosphate","id":"ChEBI:CHEBI:57642"}},{"type":"Binding site","location":{"start":{"value":287,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57642"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"dihydroxyacetone phosphate","id":"ChEBI:CHEBI:57642"}},{"type":"Modified residue","location":{"start":{"value":11,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17287358"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17330950"}]},{"type":"Modified residue","location":{"start":{"value":56,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":76,"modifier":"EXACT"},"end":{"value":76,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18407956"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18407956"}]},{"type":"Modified residue","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":147,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17287358"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18407956"}]},{"type":"Modified residue","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17330950"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18407956"}]},{"type":"Modified residue","location":{"start":{"value":179,"modifier":"EXACT"},"end":{"value":179,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17287358"}]},{"type":"Modified residue","location":{"start":{"value":268,"modifier":"EXACT"},"end":{"value":268,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17287358"}]},{"type":"Modified residue","location":{"start":{"value":290,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18407956"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":310,"modifier":"EXACT"},"end":{"value":310,"modifier":"EXACT"}},"description":"Phosphotyrosine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17330950"}]},{"type":"Modified residue","location":{"start":{"value":313,"modifier":"EXACT"},"end":{"value":313,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17330950"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18407956"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Cross-link","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"22106047"}]},{"type":"Cross-link","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"22106047"}]},{"type":"Cross-link","location":{"start":{"value":85,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"22106047"}]},{"type":"Cross-link","location":{"start":{"value":308,"modifier":"EXACT"},"end":{"value":308,"modifier":"EXACT"}},"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"22106047"}]}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0324","category":"Biological process","name":"Glycolysis"},{"id":"KW-1017","category":"PTM","name":"Isopeptide bond"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0832","category":"PTM","name":"Ubl conjugation"},{"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"2647491","citationType":"journal article","authors":["Schwelberger H.G.","Kohlwein S.D.","Paltauf F."],"citationCrossReferences":[{"database":"PubMed","id":"2647491"},{"database":"DOI","id":"10.1111/j.1432-1033.1989.tb14648.x"}],"title":"Molecular cloning, primary structure and disruption of the structural gene of aldolase from Saccharomyces cerevisiae.","publicationDate":"1989","journal":"Eur. J. Biochem.","firstPage":"301","lastPage":"308","volume":"180"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":2,"citation":{"id":"8196765","citationType":"journal article","authors":["Dujon B.","Alexandraki D.","Andre B.","Ansorge W.","Baladron V.","Ballesta J.P.G.","Banrevi A.","Bolle P.-A.","Bolotin-Fukuhara M.","Bossier P.","Bou G.","Boyer J.","Buitrago M.J.","Cheret G.","Colleaux L.","Daignan-Fornier B.","del Rey F.","Dion C.","Domdey H.","Duesterhoeft A.","Duesterhus S.","Entian K.-D.","Erfle H.","Esteban P.F.","Feldmann H.","Fernandes L.","Fobo G.M.","Fritz C.","Fukuhara H.","Gabel C.","Gaillon L.","Garcia-Cantalejo J.M.","Garcia-Ramirez J.J.","Gent M.E.","Ghazvini M.","Goffeau A.","Gonzalez A.","Grothues D.","Guerreiro P.","Hegemann J.H.","Hewitt N.","Hilger F.","Hollenberg C.P.","Horaitis O.","Indge K.J.","Jacquier A.","James C.M.","Jauniaux J.-C.","Jimenez A.","Keuchel H.","Kirchrath L.","Kleine K.","Koetter P.","Legrain P.","Liebl S.","Louis E.J.","Maia e Silva A.","Marck C.","Monnier A.-L.","Moestl D.","Mueller S.","Obermaier B.","Oliver S.G.","Pallier C.","Pascolo S.","Pfeiffer F.","Philippsen P.","Planta R.J.","Pohl F.M.","Pohl T.M.","Poehlmann R.","Portetelle D.","Purnelle B.","Puzos V.","Ramezani Rad M.","Rasmussen S.W.","Remacha M.A.","Revuelta J.L.","Richard G.-F.","Rieger M.","Rodrigues-Pousada C.","Rose M.","Rupp T.","Santos M.A.","Schwager C.","Sensen C.","Skala J.","Soares H.","Sor F.","Stegemann J.","Tettelin H.","Thierry A.","Tzermia M.","Urrestarazu L.A.","van Dyck L.","van Vliet-Reedijk J.C.","Valens M.","Vandenbol M.","Vilela C.","Vissers S.","von Wettstein D.","Voss H.","Wiemann S.","Xu G.","Zimmermann J.","Haasemann M.","Becker I.","Mewes H.-W."],"citationCrossReferences":[{"database":"PubMed","id":"8196765"},{"database":"DOI","id":"10.1038/369371a0"}],"title":"Complete DNA sequence of yeast chromosome XI.","publicationDate":"1994","journal":"Nature","firstPage":"371","lastPage":"378","volume":"369"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"8091862","citationType":"journal article","authors":["Rasmussen S.W."],"citationCrossReferences":[{"database":"PubMed","id":"8091862"},{"database":"DOI","id":"10.1002/yea.320100008"}],"title":"Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins.","publicationDate":"1994","journal":"Yeast","firstPage":"S63","lastPage":"S68","volume":"10"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"1698168","citationType":"journal article","authors":["Gatignol A.","Dassain M.","Tiraby G."],"citationCrossReferences":[{"database":"PubMed","id":"1698168"},{"database":"DOI","id":"10.1016/0378-1119(90)90159-o"}],"title":"Cloning of Saccharomyces cerevisiae promoters using a probe vector based on phleomycin resistance.","publicationDate":"1990","journal":"Gene","firstPage":"35","lastPage":"41","volume":"91"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-96"]},{"referenceNumber":6,"citation":{"id":"CI-CDGECIG83EA0A","citationType":"thesis","authors":["Jack R.S."],"publicationDate":"1973","institute":"University of Cambridge","address":"United Kingdom"},"referencePositions":["PRELIMINARY PROTEIN SEQUENCE OF 2-40"]},{"referenceNumber":7,"citation":{"id":"7895733","citationType":"journal article","authors":["Garrels J.I.","Futcher B.","Kobayashi R.","Latter G.I.","Schwender B.","Volpe T.","Warner J.R.","McLaughlin C.S."],"citationCrossReferences":[{"database":"PubMed","id":"7895733"},{"database":"DOI","id":"10.1002/elps.11501501210"}],"title":"Protein identifications for a Saccharomyces cerevisiae protein database.","publicationDate":"1994","journal":"Electrophoresis","firstPage":"1466","lastPage":"1486","volume":"15"},"referencePositions":["PROTEIN SEQUENCE OF 333-339"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":8,"citation":{"id":"8935650","citationType":"journal article","authors":["Norbeck J.","Blomberg A."],"citationCrossReferences":[{"database":"PubMed","id":"8935650"},{"database":"DOI","id":"10.1111/j.1574-6968.1996.tb08073.x"}],"title":"Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae.","publicationDate":"1996","journal":"FEMS Microbiol. Lett.","firstPage":"1","lastPage":"8","volume":"137"},"referencePositions":["PROTEIN SEQUENCE OF 2-21"],"referenceComments":[{"value":"ATCC 38531 / Y41","type":"STRAIN"}]},{"referenceNumber":9,"citation":{"id":"14562106","citationType":"journal article","authors":["Ghaemmaghami S.","Huh W.-K.","Bower K.","Howson R.W.","Belle A.","Dephoure N.","O'Shea E.K.","Weissman J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14562106"},{"database":"DOI","id":"10.1038/nature02046"}],"title":"Global analysis of protein expression in yeast.","publicationDate":"2003","journal":"Nature","firstPage":"737","lastPage":"741","volume":"425"},"referencePositions":["LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":10,"citation":{"id":"17330950","citationType":"journal article","authors":["Li X.","Gerber S.A.","Rudner A.D.","Beausoleil S.A.","Haas W.","Villen J.","Elias J.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"17330950"},{"database":"DOI","id":"10.1021/pr060559j"}],"title":"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.","publicationDate":"2007","journal":"J. Proteome Res.","firstPage":"1190","lastPage":"1197","volume":"6"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-11; THR-150; TYR-310 AND SER-313","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"ADR376","type":"STRAIN"}]},{"referenceNumber":11,"citation":{"id":"17287358","citationType":"journal article","authors":["Chi A.","Huttenhower C.","Geer L.Y.","Coon J.J.","Syka J.E.P.","Bai D.L.","Shabanowitz J.","Burke D.J.","Troyanskaya O.G.","Hunt D.F."],"citationCrossReferences":[{"database":"PubMed","id":"17287358"},{"database":"DOI","id":"10.1073/pnas.0607084104"}],"title":"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.","publicationDate":"2007","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"2193","lastPage":"2198","volume":"104"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-11; SER-147; THR-179 AND SER-268","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":12,"citation":{"id":"18407956","citationType":"journal article","authors":["Albuquerque C.P.","Smolka M.B.","Payne S.H.","Bafna V.","Eng J.","Zhou H."],"citationCrossReferences":[{"database":"PubMed","id":"18407956"},{"database":"DOI","id":"10.1074/mcp.m700468-mcp200"}],"title":"A multidimensional chromatography technology for in-depth phosphoproteome analysis.","publicationDate":"2008","journal":"Mol. Cell. Proteomics","firstPage":"1389","lastPage":"1396","volume":"7"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76; SER-96; SER-147; THR-150; THR-290 AND SER-313","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":13,"citation":{"id":"19779198","citationType":"journal article","authors":["Holt L.J.","Tuch B.B.","Villen J.","Johnson A.D.","Gygi S.P.","Morgan D.O."],"citationCrossReferences":[{"database":"PubMed","id":"19779198"},{"database":"DOI","id":"10.1126/science.1172867"}],"title":"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.","publicationDate":"2009","journal":"Science","firstPage":"1682","lastPage":"1686","volume":"325"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56; SER-63; SER-76; SER-83; THR-290 AND SER-313","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":14,"citation":{"id":"22814378","citationType":"journal article","authors":["Van Damme P.","Lasa M.","Polevoda B.","Gazquez C.","Elosegui-Artola A.","Kim D.S.","De Juan-Pardo E.","Demeyer K.","Hole K.","Larrea E.","Timmerman E.","Prieto J.","Arnesen T.","Sherman F.","Gevaert K.","Aldabe R."],"citationCrossReferences":[{"database":"PubMed","id":"22814378"},{"database":"DOI","id":"10.1073/pnas.1210303109"}],"title":"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.","publicationDate":"2012","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"12449","lastPage":"12454","volume":"109"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":15,"citation":{"id":"22106047","citationType":"journal article","authors":["Starita L.M.","Lo R.S.","Eng J.K.","von Haller P.D.","Fields S."],"citationCrossReferences":[{"database":"PubMed","id":"22106047"},{"database":"DOI","id":"10.1002/pmic.201100166"}],"title":"Sites of ubiquitin attachment in Saccharomyces cerevisiae.","publicationDate":"2012","journal":"Proteomics","firstPage":"236","lastPage":"240","volume":"12"},"referencePositions":["UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-27; LYS-73; LYS-85 AND LYS-308","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X15003","properties":[{"key":"ProteinId","value":"CAA33111.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"Z28060","properties":[{"key":"ProteinId","value":"CAA81897.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"X75781","properties":[{"key":"ProteinId","value":"CAA53412.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"M32026","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006944","properties":[{"key":"ProteinId","value":"DAA09097.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S07855","properties":[{"key":"EntryName","value":"ADBY2"}]},{"database":"RefSeq","id":"NP_012863.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001179626.1"}]},{"database":"AlphaFoldDB","id":"P14540","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P14540","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"34073","properties":[{"key":"Interactions","value":"217"}]},{"database":"DIP","id":"DIP-4702N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P14540","properties":[{"key":"Number of interactors","value":"986"}]},{"database":"IntAct","id":"P14540","properties":[{"key":"Interactions","value":"125"}]},{"database":"MINT","id":"P14540","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"4932.YKL060C","properties":[{"key":"Description","value":"-"}]},{"database":"MoonDB","id":"P14540","properties":[{"key":"Type","value":"Curated"}]},{"database":"MoonProt","id":"P14540","properties":[{"key":"Description","value":"-"}]},{"database":"CarbonylDB","id":"P14540","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"P14540","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YKL060C","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P14540","properties":[{"key":"Description","value":"-"}]},{"database":"TopDownProteomics","id":"P14540","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YKL060C_mRNA","properties":[{"key":"ProteinId","value":"YKL060C"},{"key":"GeneId","value":"YKL060C"}]},{"database":"GeneID","id":"853805","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YKL060C","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000001543","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000001543","properties":[{"key":"GeneName","value":"FBA1"}]},{"database":"VEuPathDB","id":"FungiDB:YKL060C","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG4153","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_036923_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P14540","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"PRTWGKL","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"35652at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:YKL060C-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.1.2.13","properties":[{"key":"OrganismId","value":"984"}]},{"database":"SABIO-RK","id":"P14540","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00109","properties":[{"key":"RectionId","value":"UER00183"}]},{"database":"BioGRID-ORCS","id":"853805","properties":[{"key":"hits","value":"0 hits in 10 CRISPR screens"}]},{"database":"CD-CODE","id":"0F56F502","properties":[{"key":"EntryName","value":"G-body"}]},{"database":"CD-CODE","id":"E03F929F","properties":[{"key":"EntryName","value":"Stress granule"}]},{"database":"PRO","id":"PR:P14540","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome XI"}]},{"database":"RNAct","id":"P14540","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"11914276"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16806052"}]},{"database":"GO","id":"GO:0005739","properties":[{"key":"GoTerm","value":"C:mitochondrion"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16806052"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16962558"}]},{"database":"GO","id":"GO:0004332","properties":[{"key":"GoTerm","value":"F:fructose-bisphosphate aldolase activity"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"2647491"}]},{"database":"GO","id":"GO:1904408","properties":[{"key":"GoTerm","value":"F:melatonin binding"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"31708896"}]},{"database":"GO","id":"GO:0008270","properties":[{"key":"GoTerm","value":"F:zinc ion binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0061621","properties":[{"key":"GoTerm","value":"P:canonical glycolysis"},{"key":"GoEvidenceType","value":"IMP:FlyBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"8435847"}]},{"database":"GO","id":"GO:0006094","properties":[{"key":"GoTerm","value":"P:gluconeogenesis"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"6384192"}]},{"database":"GO","id":"GO:0006096","properties":[{"key":"GoTerm","value":"P:glycolytic process"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"6384192"}]},{"database":"CDD","id":"cd00946","properties":[{"key":"EntryName","value":"FBP_aldolase_IIA"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.70:FF:000013","properties":[{"key":"EntryName","value":"Class II fructose-bisphosphate aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR000771","properties":[{"key":"EntryName","value":"FBA_II"}]},{"database":"InterPro","id":"IPR006411","properties":[{"key":"EntryName","value":"Fruct_bisP_bact"}]},{"database":"NCBIfam","id":"TIGR00167","properties":[{"key":"EntryName","value":"cbbA"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01520","properties":[{"key":"EntryName","value":"FruBisAldo_II_A"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF006628","properties":[{"key":"EntryName","value":"PRK09197.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30559:SF0","properties":[{"key":"EntryName","value":"FRUCTOSE-BISPHOSPHATE ALDOLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30559","properties":[{"key":"EntryName","value":"FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01116","properties":[{"key":"EntryName","value":"F_bP_aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF001359","properties":[{"key":"EntryName","value":"F_bP_aldolase_II"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00602","properties":[{"key":"EntryName","value":"ALDOLASE_CLASS_II_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00806","properties":[{"key":"EntryName","value":"ALDOLASE_CLASS_II_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGVEQILKRKTGVIVGEDVHNLFTYAKEHKFAIPAINVTSSSTAVAALEAARDSKSPIILQTSNGGAAYFAGKGISNEGQNASIKGAIAAAHYIRSIAPAYGIPVVLHSDHCAKKLLPWFDGMLEADEAYFKEHGEPLFSSHMLDLSEETDEENISTCVKYFKRMAAMDQWLEMEIGITGGEEDGVNNENADKEDLYTKPEQVYNVYKALHPISPNFSIAAAFGNCHGLYAGDIALRPEILAEHQKYTREQVGCKEEKPLFLVFHGGSGSTVQEFHTGIDNGVVKVNLDTDCQYAYLTGIRDYVLNKKDYIMSPVGNPEGPEKPNKKFFDPRVWVREGEKTMGAKITKSLETFRTTNTL","length":359,"molWeight":39621,"crc64":"C67E61BA5C7E8E4C","md5":"719BFF1A0E6095A1CCC892C10798D16B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"PATHWAY":1,"SUBUNIT":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Initiator methionine":1,"Chain":1,"Active site":1,"Binding site":9,"Modified residue":13,"Cross-link":4},"uniParcId":"UPI000004F935"}}