{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P11519","uniProtkbId":"FLMC1_ECOLI","entryAudit":{"firstPublicDate":"1989-10-01","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"1989-10-01","entryVersion":86,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Protein FlmC"}},"alternativeNames":[{"fullName":{"value":"F-plasmid maintenance protein C"}}]},"genes":[{"geneName":{"value":"flmC"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"3049248"}],"value":"ORF70"}],"orderedLocusNames":[{"value":"ECOK12F065"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"3049248"}],"value":"Component of a type I toxin-antitoxin (TA) system (Probable). Either this protein or sequences upstream of it are required for translation of downstream flmA; this could be translationally coupled to flmA (PubMed:3049248)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"3049248"}],"value":"Part of the flmC-flmA operon; the antisense antitoxin RNA flmB of this system overlaps the ribosome-binding site and first 3 codons of this gene on the opposite strand"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"3049248"}],"value":"A plasmid unable to express the start codon of this protein can be lost without detriment to the bacteria, when normally the plasmid is not lost due to the Flm plasmid maintenance system"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"Protein FlmC","featureId":"PRO_0000068355"}],"geneLocations":[{"value":"F","geneEncodingType":"Plasmid"}],"keywords":[{"id":"KW-0614","category":"Technical term","name":"Plasmid"},{"id":"KW-1277","category":"Biological process","name":"Toxin-antitoxin system"}],"references":[{"referenceNumber":1,"citation":{"id":"3049248","citationType":"journal article","authors":["Loh S.M.","Cram D.S.","Skurray R.A."],"citationCrossReferences":[{"database":"PubMed","id":"3049248"},{"database":"DOI","id":"10.1016/0378-1119(88)90362-9"}],"title":"Nucleotide sequence and transcriptional analysis of a third function (Flm) involved in F-plasmid maintenance.","publicationDate":"1988","journal":"Gene","firstPage":"259","lastPage":"268","volume":"66"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"K12 / AB1157","type":"STRAIN"},{"value":"F","type":"PLASMID"}]},{"referenceNumber":2,"citation":{"id":"CI-CTLQDQV8LTQ4B","citationType":"submission","authors":["Shimizu H.","Saitoh Y.","Suda Y.","Uehara K.","Sampei G.","Mizobuchi K."],"title":"Complete nucleotide sequence of the F plasmid: its implications for organization and diversification of plasmid genomes.","publicationDate":"APR-2000","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / CR63","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF106329","properties":[{"key":"ProteinId","value":"AAA99215.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP001918","properties":[{"key":"ProteinId","value":"BAA97935.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"JS0433","properties":[{"key":"EntryName","value":"JS0433"}]},{"database":"AlphaFoldDB","id":"P11519","properties":[{"key":"Description","value":"-"}]},{"database":"InterPro","id":"IPR035273","properties":[{"key":"EntryName","value":"DUF5431"}]},{"database":"NCBIfam","id":"NF010277","properties":[{"key":"EntryName","value":"PRK13720.1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17496","properties":[{"key":"EntryName","value":"DUF5431"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLRQHQDSLLLRFAQGEEGHETTTQLSCLVCVDRVSHTVDIHLSDTKIAVRDSLQRRIQGGGGFHGLRIR","length":70,"molWeight":7894,"crc64":"DABF895A00DCF3B6","md5":"D98D348135DC476BFFDC3FA374D56F77"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI000012A8E4"}}