{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P11075","secondaryAccessions":["D6VSF1","Q03960","Q04139"],"uniProtkbId":"SEC7_YEAST","entryAudit":{"firstPublicDate":"1989-07-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-11-01","entryVersion":213,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"2684655"}],"value":"ADP-ribosylation factor guanine nucleotide-exchange factor SEC7"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22516198"}],"value":"ARF-GEP SEC7"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"3042778"}],"value":"SEC7"},"orderedLocusNames":[{"value":"YDR170C"}],"orfNames":[{"value":"YD9395.01C"},{"value":"YD9489.05C"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11208097"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1729652"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1986005"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22516198"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24613384"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25220393"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26360031"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2684655"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29514977"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"3042778"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9473503"}],"value":"Guanine exchange factor that acts as an activator of ARF1 at the trans-Golgi network and is thus involved in vesicular budding and traffic between compartments of the Golgi apparatus (PubMed:11208097, PubMed:1729652, PubMed:1986005, PubMed:22516198, PubMed:24613384, PubMed:26360031, PubMed:2684655, PubMed:29514977, PubMed:3042778). Activation of Arf (ADP-ribosylation factor) GTPases is essential for vesicle formation via recruitment of cargo adapters and coat proteins necessary for Golgi trafficking (PubMed:26360031). Also plays an essential role in ER-to-Golgi traffic (PubMed:9473503). SEC7 also acts as an effector of two Rab GTPases, YPT1 and YPT31/32 (PubMed:25220393)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18832381"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22516198"}],"value":"Interacts with ARF1 (PubMed:22516198). Interacts (via C-terminus) with RSP5 ubiquitin ligase (PubMed:18832381)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22516198"}],"value":"Recruited to the trans-Golgi network by positive feedback via its HDS1 domain by interaction with activated ARF1"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1986005"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18832381"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1986005"}],"value":"Golgi apparatus","id":"SL-0132"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22516198"}],"value":"Golgi apparatus, trans-Golgi network","id":"SL-0266"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11208097"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1729652"}],"value":"Cytoplasmic vesicle, COPI-coated vesicle membrane","id":"SL-0076"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11208097"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1729652"}],"value":"Cytoplasmic vesicle, COPII-coated vesicle membrane","id":"SL-0078"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1986005"}],"value":"The highly charged acidic domain may serve a structural role to interact with lipids or proteins on the cytoplasmic surface of the Golgi apparatus"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29514977"}],"value":"The homology upstream of Sec7 (HUS) box is necessary for the allosteric activation of SEC7 by YPT31"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22516198"}],"value":"The homology downstream of Sec7 (HDS) domain 1 is required for recruitment to the trans-Golgi network via its interaction with activated ARF1"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18832381"}],"value":"A 50-amino acid region near the C-terminus called the as the C2 domain-interacting region (CIR) is required for RSP5-binding and for normal SEC7 localization"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562106"}],"value":"Present with 3670 molecules/cell in log phase SD medium"}],"commentType":"MISCELLANEOUS"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":2009,"modifier":"EXACT"}},"description":"ADP-ribosylation factor guanine nucleotide-exchange factor SEC7","featureId":"PRO_0000120214"},{"type":"Domain","location":{"start":{"value":824,"modifier":"EXACT"},"end":{"value":1010,"modifier":"EXACT"}},"description":"SEC7","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00189"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":771,"modifier":"EXACT"},"end":{"value":814,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1017,"modifier":"EXACT"},"end":{"value":1220,"modifier":"EXACT"}},"description":"HDS1 domain","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22516198"}]},{"type":"Region","location":{"start":{"value":1708,"modifier":"EXACT"},"end":{"value":1803,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1836,"modifier":"EXACT"},"end":{"value":1883,"modifier":"EXACT"}},"description":"C2 domain-interacting region (CIR)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18832381"}]},{"type":"Motif","location":{"start":{"value":653,"modifier":"EXACT"},"end":{"value":657,"modifier":"EXACT"}},"description":"HUS box","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29514977"}]},{"type":"Compositional bias","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":91,"modifier":"EXACT"},"end":{"value":118,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":134,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":144,"modifier":"EXACT"},"end":{"value":154,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":194,"modifier":"EXACT"},"end":{"value":209,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":210,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":771,"modifier":"EXACT"},"end":{"value":788,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1708,"modifier":"EXACT"},"end":{"value":1723,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1724,"modifier":"EXACT"},"end":{"value":1751,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1764,"modifier":"EXACT"},"end":{"value":1777,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":940,"modifier":"EXACT"},"end":{"value":940,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"4OIY"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Modified residue","location":{"start":{"value":212,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"15665377"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17330950"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":215,"modifier":"EXACT"},"end":{"value":215,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17330950"}]},{"type":"Modified residue","location":{"start":{"value":334,"modifier":"EXACT"},"end":{"value":334,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18407956"}]},{"type":"Modified residue","location":{"start":{"value":447,"modifier":"EXACT"},"end":{"value":447,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18407956"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":452,"modifier":"EXACT"},"end":{"value":452,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":455,"modifier":"EXACT"},"end":{"value":455,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":807,"modifier":"EXACT"},"end":{"value":807,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17330950"}]},{"type":"Modified residue","location":{"start":{"value":1226,"modifier":"EXACT"},"end":{"value":1226,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17330950"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18407956"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":1240,"modifier":"EXACT"},"end":{"value":1240,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17330950"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":1741,"modifier":"EXACT"},"end":{"value":1741,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":1752,"modifier":"EXACT"},"end":{"value":1752,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Cross-link","location":{"start":{"value":797,"modifier":"EXACT"},"end":{"value":797,"modifier":"EXACT"}},"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"22106047"}]},{"type":"Mutagenesis","location":{"start":{"value":653,"modifier":"EXACT"},"end":{"value":653,"modifier":"EXACT"}},"description":"Prevents stimulation by YPT31 and leads to lethality when ARF1 is absent.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29514977"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":655,"modifier":"EXACT"},"end":{"value":655,"modifier":"EXACT"}},"description":"Leads to lethality when ARF1 is absent.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29514977"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":188,"modifier":"EXACT"},"end":{"value":188,"modifier":"EXACT"}},"description":"in Ref. 1; AAB04031","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":399,"modifier":"EXACT"},"end":{"value":400,"modifier":"EXACT"}},"description":"in Ref. 1; AAB04031","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"FV","alternativeSequences":["LL"]}},{"type":"Sequence conflict","location":{"start":{"value":402,"modifier":"EXACT"},"end":{"value":402,"modifier":"EXACT"}},"description":"in Ref. 1; AAB04031","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["C"]}},{"type":"Sequence conflict","location":{"start":{"value":1031,"modifier":"EXACT"},"end":{"value":1034,"modifier":"EXACT"}},"description":"in Ref. 1; AAB04031","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"QQSA","alternativeSequences":["PAIC"]}},{"type":"Sequence conflict","location":{"start":{"value":1036,"modifier":"EXACT"},"end":{"value":1037,"modifier":"EXACT"}},"description":"in Ref. 1; AAB04031","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"NF","alternativeSequences":["QL"]}},{"type":"Helix","location":{"start":{"value":826,"modifier":"EXACT"},"end":{"value":829,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Helix","location":{"start":{"value":830,"modifier":"EXACT"},"end":{"value":840,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Helix","location":{"start":{"value":842,"modifier":"EXACT"},"end":{"value":851,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Beta strand","location":{"start":{"value":854,"modifier":"EXACT"},"end":{"value":856,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Helix","location":{"start":{"value":860,"modifier":"EXACT"},"end":{"value":869,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Helix","location":{"start":{"value":875,"modifier":"EXACT"},"end":{"value":883,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Helix","location":{"start":{"value":887,"modifier":"EXACT"},"end":{"value":898,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Helix","location":{"start":{"value":907,"modifier":"EXACT"},"end":{"value":915,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Helix","location":{"start":{"value":924,"modifier":"EXACT"},"end":{"value":941,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Turn","location":{"start":{"value":943,"modifier":"EXACT"},"end":{"value":945,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Helix","location":{"start":{"value":949,"modifier":"EXACT"},"end":{"value":967,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Beta strand","location":{"start":{"value":970,"modifier":"EXACT"},"end":{"value":972,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Helix","location":{"start":{"value":973,"modifier":"EXACT"},"end":{"value":976,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Helix","location":{"start":{"value":977,"modifier":"EXACT"},"end":{"value":983,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Turn","location":{"start":{"value":984,"modifier":"EXACT"},"end":{"value":987,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Helix","location":{"start":{"value":992,"modifier":"EXACT"},"end":{"value":994,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]},{"type":"Helix","location":{"start":{"value":995,"modifier":"EXACT"},"end":{"value":1007,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OIY"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0968","category":"Cellular component","name":"Cytoplasmic vesicle"},{"id":"KW-0333","category":"Cellular component","name":"Golgi apparatus"},{"id":"KW-1017","category":"PTM","name":"Isopeptide bond"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0813","category":"Biological process","name":"Transport"},{"id":"KW-0832","category":"PTM","name":"Ubl conjugation"}],"references":[{"referenceNumber":1,"citation":{"id":"3042778","citationType":"journal article","authors":["Achstetter T.","Franzusoff A.","Field C.","Schekman R."],"citationCrossReferences":[{"database":"PubMed","id":"3042778"},{"database":"DOI","id":"10.1016/s0021-9258(18)37842-6"}],"title":"SEC7 encodes an unusual, high molecular weight protein required for membrane traffic from the yeast Golgi apparatus.","publicationDate":"1988","journal":"J. Biol. Chem.","firstPage":"11711","lastPage":"11717","volume":"263"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION"]},{"referenceNumber":2,"citation":{"id":"9169867","citationType":"journal article","authors":["Jacq C.","Alt-Moerbe J.","Andre B.","Arnold W.","Bahr A.","Ballesta J.P.G.","Bargues M.","Baron L.","Becker A.","Biteau N.","Bloecker H.","Blugeon C.","Boskovic J.","Brandt P.","Brueckner M.","Buitrago M.J.","Coster F.","Delaveau T.","del Rey F.","Dujon B.","Eide L.G.","Garcia-Cantalejo J.M.","Goffeau A.","Gomez-Peris A.","Granotier C.","Hanemann V.","Hankeln T.","Hoheisel J.D.","Jaeger W.","Jimenez A.","Jonniaux J.-L.","Kraemer C.","Kuester H.","Laamanen P.","Legros Y.","Louis E.J.","Moeller-Rieker S.","Monnet A.","Moro M.","Mueller-Auer S.","Nussbaumer B.","Paricio N.","Paulin L.","Perea J.","Perez-Alonso M.","Perez-Ortin J.E.","Pohl T.M.","Prydz H.","Purnelle B.","Rasmussen S.W.","Remacha M.A.","Revuelta J.L.","Rieger M.","Salom D.","Saluz H.P.","Saiz J.E.","Saren A.-M.","Schaefer M.","Scharfe M.","Schmidt E.R.","Schneider C.","Scholler P.","Schwarz S.","Soler-Mira A.","Urrestarazu L.A.","Verhasselt P.","Vissers S.","Voet M.","Volckaert G.","Wagner G.","Wambutt R.","Wedler E.","Wedler H.","Woelfl S.","Harris D.E.","Bowman S.","Brown D.","Churcher C.M.","Connor R.","Dedman K.","Gentles S.","Hamlin N.","Hunt S.","Jones L.","McDonald S.","Murphy L.D.","Niblett D.","Odell C.","Oliver K.","Rajandream M.A.","Richards C.","Shore L.","Walsh S.V.","Barrell B.G.","Dietrich F.S.","Mulligan J.T.","Allen E.","Araujo R.","Aviles E.","Berno A.","Carpenter J.","Chen E.","Cherry J.M.","Chung E.","Duncan M.","Hunicke-Smith S.","Hyman R.W.","Komp C.","Lashkari D.","Lew H.","Lin D.","Mosedale D.","Nakahara K.","Namath A.","Oefner P.","Oh C.","Petel F.X.","Roberts D.","Schramm S.","Schroeder M.","Shogren T.","Shroff N.","Winant A.","Yelton M.A.","Botstein D.","Davis R.W.","Johnston M.","Andrews S.","Brinkman R.","Cooper J.","Ding H.","Du Z.","Favello A.","Fulton L.","Gattung S.","Greco T.","Hallsworth K.","Hawkins J.","Hillier L.W.","Jier M.","Johnson D.","Johnston L.","Kirsten J.","Kucaba T.","Langston Y.","Latreille P.","Le T.","Mardis E.","Menezes S.","Miller N.","Nhan M.","Pauley A.","Peluso D.","Rifkin L.","Riles L.","Taich A.","Trevaskis E.","Vignati D.","Wilcox L.","Wohldman P.","Vaudin M.","Wilson R.","Waterston R.","Albermann K.","Hani J.","Heumann K.","Kleine K.","Mewes H.-W.","Zollner A.","Zaccaria P."],"citationCrossReferences":[{"database":"PubMed","id":"9169867"},{"database":"DOI","id":"10.1038/387s075"}],"title":"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.","publicationDate":"1997","journal":"Nature","firstPage":"75","lastPage":"78","volume":"387"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"2684655","citationType":"journal article","authors":["Franzusoff A.","Schekman R."],"citationCrossReferences":[{"database":"PubMed","id":"2684655"},{"database":"DOI","id":"10.1002/j.1460-2075.1989.tb08410.x"}],"title":"Functional compartments of the yeast Golgi apparatus are defined by the sec7 mutation.","publicationDate":"1989","journal":"EMBO J.","firstPage":"2695","lastPage":"2702","volume":"8"},"referencePositions":["FUNCTION"]},{"referenceNumber":5,"citation":{"id":"1986005","citationType":"journal article","authors":["Franzusoff A.","Redding K.","Crosby J.","Fuller R.S.","Schekman R."],"citationCrossReferences":[{"database":"PubMed","id":"1986005"},{"database":"DOI","id":"10.1083/jcb.112.1.27"}],"title":"Localization of components involved in protein transport and processing through the yeast Golgi apparatus.","publicationDate":"1991","journal":"J. Cell Biol.","firstPage":"27","lastPage":"37","volume":"112"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","DOMAIN"]},{"referenceNumber":6,"citation":{"id":"1729652","citationType":"journal article","authors":["Franzusoff A.","Lauze E.","Howell K.E."],"citationCrossReferences":[{"database":"PubMed","id":"1729652"},{"database":"DOI","id":"10.1038/355173a0"}],"title":"Immuno-isolation of Sec7p-coated transport vesicles from the yeast secretory pathway.","publicationDate":"1992","journal":"Nature","firstPage":"173","lastPage":"175","volume":"355"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION"]},{"referenceNumber":7,"citation":{"id":"9473503","citationType":"journal article","authors":["Wolf J.","Nicks M.","Deitz S.","van Tuinen E.","Franzusoff A."],"citationCrossReferences":[{"database":"PubMed","id":"9473503"},{"database":"DOI","id":"10.1006/bbrc.1998.8084"}],"title":"An N-end rule destabilization mutant reveals pre-Golgi requirements for Sec7p in yeast membrane traffic.","publicationDate":"1998","journal":"Biochem. Biophys. Res. Commun.","firstPage":"191","lastPage":"198","volume":"243"},"referencePositions":["FUNCTION"]},{"referenceNumber":8,"citation":{"id":"11208097","citationType":"journal article","authors":["Deitz S.B.","Rambourg A.","Kepes F.","Franzusoff A."],"citationCrossReferences":[{"database":"PubMed","id":"11208097"},{"database":"DOI","id":"10.1034/j.1600-0854.2000.010209.x"}],"title":"Sec7p directs the transitions required for yeast Golgi biogenesis.","publicationDate":"2000","journal":"Traffic","firstPage":"172","lastPage":"183","volume":"1"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION"]},{"referenceNumber":9,"citation":{"id":"14690591","citationType":"journal article","authors":["Hazbun T.R.","Malmstroem L.","Anderson S.","Graczyk B.J.","Fox B.","Riffle M.","Sundin B.A.","Aranda J.D.","McDonald W.H.","Chiu C.-H.","Snydsman B.E.","Bradley P.","Muller E.G.D.","Fields S.","Baker D.","Yates J.R. III","Davis T.N."],"citationCrossReferences":[{"database":"PubMed","id":"14690591"},{"database":"DOI","id":"10.1016/s1097-2765(03)00476-3"}],"title":"Assigning function to yeast proteins by integration of technologies.","publicationDate":"2003","journal":"Mol. Cell","firstPage":"1353","lastPage":"1365","volume":"12"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY"]},{"referenceNumber":10,"citation":{"id":"14562106","citationType":"journal article","authors":["Ghaemmaghami S.","Huh W.-K.","Bower K.","Howson R.W.","Belle A.","Dephoure N.","O'Shea E.K.","Weissman J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14562106"},{"database":"DOI","id":"10.1038/nature02046"}],"title":"Global analysis of protein expression in yeast.","publicationDate":"2003","journal":"Nature","firstPage":"737","lastPage":"741","volume":"425"},"referencePositions":["LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":11,"citation":{"id":"15665377","citationType":"journal article","authors":["Gruhler A.","Olsen J.V.","Mohammed S.","Mortensen P.","Faergeman N.J.","Mann M.","Jensen O.N."],"citationCrossReferences":[{"database":"PubMed","id":"15665377"},{"database":"DOI","id":"10.1074/mcp.m400219-mcp200"}],"title":"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.","publicationDate":"2005","journal":"Mol. Cell. Proteomics","firstPage":"310","lastPage":"327","volume":"4"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"YAL6B","type":"STRAIN"}]},{"referenceNumber":12,"citation":{"id":"17330950","citationType":"journal article","authors":["Li X.","Gerber S.A.","Rudner A.D.","Beausoleil S.A.","Haas W.","Villen J.","Elias J.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"17330950"},{"database":"DOI","id":"10.1021/pr060559j"}],"title":"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.","publicationDate":"2007","journal":"J. Proteome Res.","firstPage":"1190","lastPage":"1197","volume":"6"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212; SER-215; SER-807; SER-1226 AND THR-1240","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"ADR376","type":"STRAIN"}]},{"referenceNumber":13,"citation":{"id":"18832381","citationType":"journal article","authors":["Dehring D.A.","Adler A.S.","Hosseini A.","Hicke L."],"citationCrossReferences":[{"database":"PubMed","id":"18832381"},{"database":"DOI","id":"10.1074/jbc.m806023200"}],"title":"A C-terminal sequence in the guanine nucleotide exchange factor Sec7 mediates Golgi association and interaction with the Rsp5 ubiquitin ligase.","publicationDate":"2008","journal":"J. Biol. Chem.","firstPage":"34188","lastPage":"34196","volume":"283"},"referencePositions":["FUNCTION","DOMAIN","INTERACTION WITH RSP5","SUBCELLULAR LOCATION"]},{"referenceNumber":14,"citation":{"id":"18407956","citationType":"journal article","authors":["Albuquerque C.P.","Smolka M.B.","Payne S.H.","Bafna V.","Eng J.","Zhou H."],"citationCrossReferences":[{"database":"PubMed","id":"18407956"},{"database":"DOI","id":"10.1074/mcp.m700468-mcp200"}],"title":"A multidimensional chromatography technology for in-depth phosphoproteome analysis.","publicationDate":"2008","journal":"Mol. Cell. Proteomics","firstPage":"1389","lastPage":"1396","volume":"7"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-334; SER-447 AND SER-1226","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":15,"citation":{"id":"19779198","citationType":"journal article","authors":["Holt L.J.","Tuch B.B.","Villen J.","Johnson A.D.","Gygi S.P.","Morgan D.O."],"citationCrossReferences":[{"database":"PubMed","id":"19779198"},{"database":"DOI","id":"10.1126/science.1172867"}],"title":"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.","publicationDate":"2009","journal":"Science","firstPage":"1682","lastPage":"1686","volume":"325"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212; SER-447; SER-452; SER-455; SER-1226; THR-1240; SER-1741 AND SER-1752","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":16,"citation":{"id":"22516198","citationType":"journal article","authors":["Richardson B.C.","McDonold C.M.","Fromme J.C."],"citationCrossReferences":[{"database":"PubMed","id":"22516198"},{"database":"DOI","id":"10.1016/j.devcel.2012.02.006"}],"title":"The Sec7 Arf-GEF is recruited to the trans-Golgi network by positive feedback.","publicationDate":"2012","journal":"Dev. Cell","firstPage":"799","lastPage":"810","volume":"22"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","DOMAIN","INTERACTION WITH ARF1"]},{"referenceNumber":17,"citation":{"id":"22106047","citationType":"journal article","authors":["Starita L.M.","Lo R.S.","Eng J.K.","von Haller P.D.","Fields S."],"citationCrossReferences":[{"database":"PubMed","id":"22106047"},{"database":"DOI","id":"10.1002/pmic.201100166"}],"title":"Sites of ubiquitin attachment in Saccharomyces cerevisiae.","publicationDate":"2012","journal":"Proteomics","firstPage":"236","lastPage":"240","volume":"12"},"referencePositions":["UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-797","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":18,"citation":{"id":"25220393","citationType":"journal article","authors":["McDonold C.M.","Fromme J.C."],"citationCrossReferences":[{"database":"PubMed","id":"25220393"},{"database":"DOI","id":"10.1016/j.devcel.2014.07.016"}],"title":"Four GTPases differentially regulate the Sec7 Arf-GEF to direct traffic at the trans-golgi network.","publicationDate":"2014","journal":"Dev. Cell","firstPage":"759","lastPage":"767","volume":"30"},"referencePositions":["FUNCTION"]},{"referenceNumber":19,"citation":{"id":"26360031","citationType":"journal article","authors":["Richardson B.C.","Fromme J.C."],"citationCrossReferences":[{"database":"PubMed","id":"26360031"},{"database":"DOI","id":"10.1016/bs.mcb.2015.03.020"}],"title":"Biochemical methods for studying kinetic regulation of Arf1 activation by Sec7.","publicationDate":"2015","journal":"Methods Cell Biol.","firstPage":"101","lastPage":"126","volume":"130"},"referencePositions":["FUNCTION"]},{"referenceNumber":20,"citation":{"id":"29514977","citationType":"journal article","authors":["Halaby S.L.","Fromme J.C."],"citationCrossReferences":[{"database":"PubMed","id":"29514977"},{"database":"DOI","id":"10.1074/jbc.ra117.001318"}],"title":"The HUS box is required for allosteric regulation of the Sec7 Arf-GEF.","publicationDate":"2018","journal":"J. Biol. Chem.","firstPage":"6682","lastPage":"6691","volume":"293"},"referencePositions":["FUNCTION","DOMAIN","MUTAGENESIS OF ASN-653 AND ASP-655"]},{"referenceNumber":21,"citation":{"id":"24613384","citationType":"journal article","authors":["Qiu B.","Zhang K.","Wang S.","Sun F."],"citationCrossReferences":[{"database":"PubMed","id":"24613384"},{"database":"DOI","id":"10.1016/j.bbrc.2014.02.125"}],"title":"C-terminal motif within Sec7 domain regulates guanine nucleotide exchange activity via tuning protein conformation.","publicationDate":"2014","journal":"Biochem. Biophys. Res. Commun.","firstPage":"380","lastPage":"386","volume":"446"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 824-1010 IN COMPLEX WITH MG(2+)","FUNCTION"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"4OIY"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"J03918","properties":[{"key":"ProteinId","value":"AAB04031.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"Z46727","properties":[{"key":"ProteinId","value":"CAA86696.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"Z47813","properties":[{"key":"ProteinId","value":"CAA87801.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006938","properties":[{"key":"ProteinId","value":"DAA12011.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S49764","properties":[{"key":"EntryName","value":"S49764"}]},{"database":"RefSeq","id":"NP_010454.3","properties":[{"key":"NucleotideSequenceId","value":"NM_001180477.3"}]},{"database":"PDB","id":"4OIY","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.50 A"},{"key":"Chains","value":"A/B=824-1010"}]},{"database":"PDBsum","id":"4OIY","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P11075","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P11075","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"32221","properties":[{"key":"Interactions","value":"307"}]},{"database":"DIP","id":"DIP-830N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P11075","properties":[{"key":"Number of interactors","value":"1254"}]},{"database":"IntAct","id":"P11075","properties":[{"key":"Interactions","value":"64"}]},{"database":"MINT","id":"P11075","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"4932.YDR170C","properties":[{"key":"Description","value":"-"}]},{"database":"CarbonylDB","id":"P11075","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"P11075","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YDR170C","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P11075","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YDR170C_mRNA","properties":[{"key":"ProteinId","value":"YDR170C"},{"key":"GeneId","value":"YDR170C"}]},{"database":"GeneID","id":"851748","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YDR170C","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000002577","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000002577","properties":[{"key":"GeneName","value":"SEC7"}]},{"database":"VEuPathDB","id":"FungiDB:YDR170C","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG0929","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_000691_1_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P11075","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"EVMCAYI","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"18431at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-29759-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"851748","properties":[{"key":"hits","value":"1 hit in 10 CRISPR screens"}]},{"database":"EvolutionaryTrace","id":"P11075","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P11075","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome IV"}]},{"database":"RNAct","id":"P11075","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0030663","properties":[{"key":"GoTerm","value":"C:COPI-coated vesicle membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1986005"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"1986005"}]},{"database":"GO","id":"GO:0012507","properties":[{"key":"GoTerm","value":"C:ER to Golgi transport vesicle membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005794","properties":[{"key":"GoTerm","value":"C:Golgi apparatus"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18832381"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1986005"}]},{"database":"GO","id":"GO:0000139","properties":[{"key":"GoTerm","value":"C:Golgi membrane"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005798","properties":[{"key":"GoTerm","value":"C:Golgi-associated vesicle"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"1729652"}]},{"database":"GO","id":"GO:0005770","properties":[{"key":"GoTerm","value":"C:late endosome"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12006663"}]},{"database":"GO","id":"GO:0005802","properties":[{"key":"GoTerm","value":"C:trans-Golgi network"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"1986005"}]},{"database":"GO","id":"GO:0005085","properties":[{"key":"GoTerm","value":"F:guanyl-nucleotide exchange factor activity"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"10430582"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"9539714"}]},{"database":"GO","id":"GO:0000045","properties":[{"key":"GoTerm","value":"P:autophagosome assembly"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"20444982"}]},{"database":"GO","id":"GO:0140455","properties":[{"key":"GoTerm","value":"P:cytoplasm protein quality control"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"37640758"}]},{"database":"GO","id":"GO:0006888","properties":[{"key":"GoTerm","value":"P:endoplasmic reticulum to Golgi vesicle-mediated transport"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"9473503"}]},{"database":"GO","id":"GO:0006891","properties":[{"key":"GoTerm","value":"P:intra-Golgi vesicle-mediated transport"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"3042778"}]},{"database":"GO","id":"GO:0032012","properties":[{"key":"GoTerm","value":"P:regulation of ARF protein signal transduction"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00171","properties":[{"key":"EntryName","value":"Sec7"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.1000.11:FF:000003","properties":[{"key":"EntryName","value":"Brefeldin A-inhibited guanine nucleotide-exchange protein 1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.220.20:FF:000002","properties":[{"key":"EntryName","value":"Brefeldin A-inhibited guanine nucleotide-exchange protein 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.220.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.1000.11","properties":[{"key":"EntryName","value":"Arf Nucleotide-binding Site Opener,domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR016024","properties":[{"key":"EntryName","value":"ARM-type_fold"}]},{"database":"InterPro","id":"IPR032629","properties":[{"key":"EntryName","value":"DCB_dom"}]},{"database":"InterPro","id":"IPR015403","properties":[{"key":"EntryName","value":"Mon2/Sec7/BIG1-like_HDS"}]},{"database":"InterPro","id":"IPR032691","properties":[{"key":"EntryName","value":"Mon2/Sec7/BIG1-like_HUS"}]},{"database":"InterPro","id":"IPR046455","properties":[{"key":"EntryName","value":"Sec7/BIG1-like_C"}]},{"database":"InterPro","id":"IPR023394","properties":[{"key":"EntryName","value":"Sec7_C_sf"}]},{"database":"InterPro","id":"IPR000904","properties":[{"key":"EntryName","value":"Sec7_dom"}]},{"database":"InterPro","id":"IPR035999","properties":[{"key":"EntryName","value":"Sec7_dom_sf"}]},{"database":"PANTHER","id":"PTHR10663","properties":[{"key":"EntryName","value":"GUANYL-NUCLEOTIDE EXCHANGE FACTOR"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10663:SF375","properties":[{"key":"EntryName","value":"LD29171P"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF20252","properties":[{"key":"EntryName","value":"BIG2_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF16213","properties":[{"key":"EntryName","value":"DCB"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01369","properties":[{"key":"EntryName","value":"Sec7"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF09324","properties":[{"key":"EntryName","value":"Sec7-like_HDS"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF12783","properties":[{"key":"EntryName","value":"Sec7-like_HUS"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00222","properties":[{"key":"EntryName","value":"Sec7"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48371","properties":[{"key":"EntryName","value":"ARM repeat"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48425","properties":[{"key":"EntryName","value":"Sec7 domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50190","properties":[{"key":"EntryName","value":"SEC7"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSEQNSVVNAEKGDGEISSNVETASSVNPSVKPQNAIKEEAKETNGEDQKCKGPENAGSTAETKETSNDATNGMKTPEETEDTNDKRHDDEGEDGDEDEDEDEDEDEDNGDEDDEDVDSSSSETSSEDGEDSESVSGESTESSSGEDEESDESDGNTSNSSSGDESGSEEEEEEEEEEEEEENAGEPAIAHQDSVPTNDSTAPRSTHTRNISLSSNGSNTNSTIILVKTTLETILNDKDIKKNSNAQKAIERTLQKFKEFDPQTTNNPHYVDSILVFEALRASCRTKSSKVQSLALDCLSKLFSFRSLDETLLVNPPDSLASNDQRQDAADGITPPPKQKIIDAAIDTISDCFQGEGTDDRVELQIVRALSSCILEEDSSSLCHGASLLKAIRTIYNVFVFSLNPSNQGIAQATLTQIISSVYDKIDLKQSTSSAVSLSTKNHQQQSAIELSEASENAETPAPLTLENMDKLNDDEERLMDAQQPDSIAITNQDLAVKDAFLVFRVMAKICAKPLETELDMRSHAVRSKLLSLHIIYSIIKDHIDVFLSHNIFLPGKERVCFIDSIRQYLRLVLSRNAASPLAPVFEVTLEIMWLLIANLRADFVKEIPVFLTEIYFPISELTTSTSQQKRYFLSVIQRICNDPRTLVEFYLNYDCNPGMPNVMEITVDYLTRLALTRVEITQTQRSYYDEQISKSLSTYNFSQLPLLTSSNLSSSPDVGQVNLLFPLDFALKMVSLNCIVSVLRSLSSWAHKALNPNTHTANKVLLNTTSSARQESRSSLSNDVRSSIMTSNDDFKPTYEDEESRSLSSQNIDADDPTQFENLKLRKTALSECIAIFNNKPKKAIPVLIKKGFLKDDSPISIAKWLLETEGLDMAAVGDYLGEGDDKNIAIMHAFVDEFDFTGMSIVDALRSFLQSFRLPGEGQKIDRFMLKFAERFVDQNPGVFSKADTAYVLSYSLIMLNTDLHSSQIKNKMSLQEFLENNEGIDNGRDLPRDFLEGLFNEIANNEIKLISEQHQAMLSGDTNLVQQQQSAFNFFNSRDLTREAYNQVSKEISSKTELVFKNLNKNKGGPDVYYAASHVEHVKSIFETLWMSFLAALTPPFKDYDDIDTTNKCLEGLKISIKIASTFRINDARTSFVGALVQFCNLQNLEEIKVKNVNAMVILLEVALSEGNYLEGSWKDILLVVSQMERLQLISKGIDRDTVPDVAQARVANPRVSYESSRSNNTSFFDVWGKKATPTELAQEKHHNQTLSPEISKFISSSELVVLMDNIFTKSSELSGNAIVDFIKALTAVSLEEIESSENASTPRMFSLQKMVDVCYYNMDRIKLEWTPLWAVMGKAFNKIATNSNLAVVFFAIDSLRQLSMRFLDIEELSGFEFQHDFLKPFEYTVQNSGNTEVQEMIIECFRNFILTKSESIKSGWKPILESLQYTARSSTESIVLKTQLLVSNDIVTNHFENVFSQEDAFSELVGVFREITKNKRFQKLSLHALESLRKMTQNVADICFYNENKTEEERKHNDALLRGKDIFQDVWFPMLFCFNDTIMTAEDLEVRSRALNYMFDALVAYGGKFNDDFWEKICKKLLFPIFGVLSKHWEVNQFNSHDDLSVWLSTTLIQALRNLIALFTHYFESLNRMLDGFLGLLVSCICQENDTIARIGRSCLQQLILQNVSKFNEYHWNQIGDVFDKLFDLTTANELFDYDPLQQGRKSSVSHHQTTNDTSQHSDDDSNDRRENDSNISETVERAHQEESSEDVGGDMVETLNGQTKLNNGNSVPTVKDELNPKPASLSIPKKTKHMKRNESNEDIRRRINIKNSIVVKCVLQLLMIELLNELFENEDFAHCIPYKEAIRITRLLEKSYEFSRDFNEDYGLRTRLVEARVVDKIPNLLKQETSAAAVLLDIMFQLYLNDDEKKADLITRLITICIQVVEGYVSLDDRTMERSINAWRSVIVEILQGYYEFDDEDFRLYCPAMYALVIQILDKSVPTELRHAIKQFLSRVGELYLSTD","length":2009,"molWeight":226886,"crc64":"02B2D370DD2E4661","md5":"DF72C59FCF50FD890000D8DC6797AF38"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DOMAIN":4,"MISCELLANEOUS":1},"countByFeatureType":{"Chain":1,"Domain":1,"Region":5,"Motif":1,"Compositional bias":13,"Binding site":1,"Modified residue":11,"Cross-link":1,"Mutagenesis":2,"Sequence conflict":5,"Helix":13,"Beta strand":2,"Turn":2},"uniParcId":"UPI0000135756"}}