{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0DXZ1","uniProtkbId":"DGCN_PSEAS","entryAudit":{"firstPublicDate":"2025-04-09","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2025-04-09","entryVersion":5,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Pseudoalteromonas sp","taxonId":53249,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Alteromonadales","Pseudoalteromonadaceae","Pseudoalteromonas"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"D-glutamate N-acetyltransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36690779"}],"value":"2.3.1.312"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"36690779"}],"value":"dgcN"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36690779"}],"value":"N-acetyltransferase involved in a deamination-independent D-glutamate degradation pathway, named the DgcN-DgcA pathway (PubMed:36690779). Catalyzes the transfer of the acetyl moiety from acetyl-CoA to D-glutamate to generate N-acetyl-D-glutamate (PubMed:36690779). Can also acetylate D-aspartate and D-glutamine, with lower efficiency (PubMed:36690779). Has low activity with D-asparagine (PubMed:36690779). Cannot use succinyl-CoA (PubMed:36690779)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"D-glutamate + acetyl-CoA = N-acetyl-D-glutamate + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:76495"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29986"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"},{"database":"ChEBI","id":"CHEBI:195260"}],"ecNumber":"2.3.1.312","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36690779"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:76496"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36690779"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"D-aspartate + acetyl-CoA = N-acetyl-D-aspartate + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:76551"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29990"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"},{"database":"ChEBI","id":"CHEBI:195274"}],"ecNumber":"2.3.1.312","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36690779"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"D-glutamine + acetyl-CoA = N-acetyl-D-glutamine + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:76547"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"},{"database":"ChEBI","id":"CHEBI:58000"},{"database":"ChEBI","id":"CHEBI:143880"}],"ecNumber":"2.3.1.312","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36690779"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36690779"}],"value":"Amino-acid degradation"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36690779"}],"value":"Expression is up-regulated in the presence of D-glutamate (PubMed:36690779). Transcriptionally regulated by DgcR (PubMed:36690779)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36690779"}],"value":"The growth of the deletion mutant is significantly reduced with D-glutamate as the sole nitrogen source"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the N-acetyltransferase DgcN family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":332,"modifier":"EXACT"}},"description":"D-glutamate N-acetyltransferase","featureId":"PRO_0000462124"}],"keywords":[{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"36690779","citationType":"journal article","authors":["Yu Y.","Wang P.","Cao H.Y.","Teng Z.J.","Zhu Y.","Wang M.","McMinn A.","Chen Y.","Xiang H.","Zhang Y.Z.","Chen X.L.","Zhang Y.Q."],"citationCrossReferences":[{"database":"PubMed","id":"36690779"},{"database":"DOI","id":"10.1038/s41396-023-01364-6"}],"title":"Novel D-glutamate catabolic pathway in marine Proteobacteria and halophilic archaea.","publicationDate":"2023","journal":"ISME J.","firstPage":"537","lastPage":"548","volume":"17"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION","CATALYTIC ACTIVITY","INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"CF6-2","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"ON505816","properties":[{"key":"ProteinId","value":"USH59575.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"P0DXZ1","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0DXZ1","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ag:USH59575","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"2.3.1.312","properties":[{"key":"OrganismId","value":"18954"}]},{"database":"Gene3D","id":"3.40.50.720","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-like Domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR011669","properties":[{"key":"EntryName","value":"DgcN-like"}]},{"database":"InterPro","id":"IPR035086","properties":[{"key":"EntryName","value":"DgcN-like_C"}]},{"database":"InterPro","id":"IPR035402","properties":[{"key":"EntryName","value":"DgcN-like_N"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"NCBIfam","id":"NF041892","properties":[{"key":"EntryName","value":"DgcN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR40690:SF1","properties":[{"key":"EntryName","value":"DUF1611 DOMAIN-CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR40690","properties":[{"key":"EntryName","value":"GLL3100 PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07755","properties":[{"key":"EntryName","value":"DUF1611"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17396","properties":[{"key":"EntryName","value":"DUF1611_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF026760","properties":[{"key":"EntryName","value":"UCP026760"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MQIKPPYLLFIGDATDKLSIKMAQSLADWRAELCVGELSVNGCTVSTGLNQCSICEAAKLGAKTFVLGFANSGGVLDKKWLPIISEAIENGMNIVSGLHDKLTNFDELVSLSQKHNTNLLDIRHPSTAFATGKGYKRKGKRLLTVGTDCSVGKMYTSLSLEKAMKEQNIDVDFRATGQCGILISGSGVAIDCVIADFISGAAESLSPDAEETHWDIIEGQGSLSHPAFAGVSLGLLHGSQPDALVICHALNRTHMRGLLHTSFPSIETTIELNIAAAKLTNPDVQVVGISVNTSSVSIEQGNEICERLSQTFGVPCVDPLRDGVDSIVANLC","length":332,"molWeight":35313,"crc64":"75E7BBE10F00CE62","md5":"989AADF84560C1A160AD1B6A377FF9EC"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":3,"PATHWAY":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI001EF07447"}}