{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0DXE6","uniProtkbId":"CGDB_MICSX","entryAudit":{"firstPublicDate":"2024-10-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2024-10-02","entryVersion":7,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Microbacterium sp","taxonId":51671,"lineage":["Bacteria","Bacillati","Actinomycetota","Actinomycetes","Micrococcales","Microbacteriaceae","Microbacterium"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"3'-dehydrocarminate deglycosidase beta subunit"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"4.1.99.28"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34728636"}],"value":"C-deglycosylation enzyme beta subunit"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34728636"}],"value":"C-glycoside deglycosidase beta subunit"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34728636"}],"value":"CGD beta subunit"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34728636"}],"value":"MiCGD beta"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34728636"}],"value":"carC"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"Carbon-carbon bond-cleaving enzyme which participates in a carminate degradation pathway (PubMed:34728636). Cleaves the C-C bond in 3'-dehydrocarminate to form kermesate (PubMed:34728636). Also shows weak activity with other C-glycosides, such as 3''-dehydropuerarin (3''-oxo-puerarin), 3''-dehydroisoorientin (3''-oxo-homoorientin) and 3'-dehydromangiferin (3'-oxo-mangiferin) (PubMed:34728636)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3'-dehydrocarminate + H(+) = kermesate + 1,5-anhydro-D-erythro-hex-1-en-3-ulose","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:76567"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:149530"},{"database":"ChEBI","id":"CHEBI:192513"},{"database":"ChEBI","id":"CHEBI:195275"}],"ecNumber":"4.1.99.28","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"Activity is strongly reduced in the presence of chelating agents"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":0.39,"unit":"mM","substrate":"3'-dehydrocarminate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}]},{"constant":7.5,"unit":"mM","substrate":"3''-dehydropuerarin","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}]},{"constant":11.0,"unit":"mM","substrate":"3''-dehydroisoorientin","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}]},{"constant":5.4,"unit":"mM","substrate":"3'-dehydromangiferin","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"kcat is 23 min(-1) with 3'-dehydrocarminate as substrate. kcat is 0.12 min(-1) with 3''-dehydropuerarin as substrate. kcat is 0.024 min(-1) with 3''-dehydroisoorientin as substrate. kcat is 0.16 min(-1) with 3'-dehydromangiferin as substrate."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"Optimum pH is 7.5."}]},"temperatureDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"Optimum temperature is around 40 degrees Celsius."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"Heterodimer composed of an alpha subunit (CarB) and a beta subunit (CarC)"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the C-glycoside deglycosidase beta subunit family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"3'-dehydrocarminate deglycosidase beta subunit","featureId":"PRO_0000461031"}],"keywords":[{"id":"KW-0119","category":"Biological process","name":"Carbohydrate metabolism"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"}],"references":[{"referenceNumber":1,"citation":{"id":"34728636","citationType":"journal article","authors":["Mori T.","Kumano T.","He H.","Watanabe S.","Senda M.","Moriya T.","Adachi N.","Hori S.","Terashita Y.","Kawasaki M.","Hashimoto Y.","Awakawa T.","Senda T.","Abe I.","Kobayashi M."],"citationCrossReferences":[{"database":"PubMed","id":"34728636"},{"database":"DOI","id":"10.1038/s41467-021-26585-1"}],"title":"C-Glycoside metabolism in the gut and in nature: Identification, characterization, structural analyses and distribution of C-C bond-cleaving enzymes.","publicationDate":"2021","journal":"Nat. Commun.","firstPage":"6294","lastPage":"6294","volume":"12"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION","CATALYTIC ACTIVITY","COFACTOR","ACTIVITY REGULATION","BIOPHYSICOCHEMICAL PROPERTIES","SUBUNIT"],"referenceComments":[{"value":"5-2b","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LC387600","properties":[{"key":"ProteinId","value":"BBE36492.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"P0DXE6","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0DXE6","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ag:BBE36492","properties":[{"key":"Description","value":"-"}]},{"database":"InterPro","id":"IPR045959","properties":[{"key":"EntryName","value":"CGDB"}]},{"database":"Pfam","id":"PF19906","properties":[{"key":"EntryName","value":"CGDB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFRERIIVQDSLVADESGSAFRIRLPWYRALPLSTIEELSVTVDGTAFDPARLRIAVNDGEWALAEAQLRTDDVWFVLDDATVRLPGLVLDAGAHEVQATLSMRIPYLPVAGKPLSMAETDQKRMDVKELTA","length":132,"molWeight":14683,"crc64":"CC5C42B95A52C776","md5":"210A403C715EE5A73B15F37D559CFDC7"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"ACTIVITY REGULATION":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI003390784B"}}