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CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophage (PubMed:37968393). The CD-NTase protein synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals (PubMed:37968393). The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection (PubMed:37968393). The effector for this system is downstream Cap15 (PubMed:37968393). A type I-B CBASS system (PubMed:32839535)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37968393"}],"value":"Cyclic dinucleotide synthase that catalyzes the synthesis of 3',2'-cyclic GMP-AMP (cGAMP) from GTP and ATP upon activation by viral-derived cabRNA (PubMed:37968393). Binds cabRNA via positive charges in its N-terminus (PubMed:37968393)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37968393"}],"value":"Protects S.aureus against phage infection. When the CBASS operon (cdnE-cap15) is introduced in S.aureus strain RN4220 there is strong protection against lytic DNA phages 80alpha-vir and phi-NM1-gamma-6 but little to no protection against phages phi-NM4-gamma-4 or phi-12-gamma-3 (PubMed:37968393)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"GTP + ATP = 3',2'-cGAMP + 2 diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:68344"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:37565"},{"database":"ChEBI","id":"CHEBI:177334"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37968393"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:68345"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37968393"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DSP2"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DSP2"}],"value":"Binds 1 Mg(2+) ion per subunit."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37968393"}],"value":"Activated by a virus-derived, approximately 400 nucleotide RNA (called CBASS-activating bacteriophage RNA, cabRNA) that begins in the viral terminase subunit terS and extends into terL (PubMed:37968393). RNA secondary and/or tertiary structure, as well as viral infection itself, are important for CdnE activation (PubMed:37968393). A much longer RNA (escaper RNA) with a different secondary structure, derived from a terS-mutated virus still binds to this protein, but does not activate its nucleotide cyclase activity (PubMed:37968393). Shorter viral-derived RNAs (34 and 49 nt) with extensive predicted secondary structure also activate the enzyme, although not as well as full-length cabRNA (PubMed:37968393)"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"30787435"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"32839535"}],"value":"Belongs to the CD-NTase family. E03 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":324,"modifier":"EXACT"}},"description":"Cyclic GMP-AMP synthase CdnE03","featureId":"PRO_0000459831"},{"type":"Binding site","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DSP2"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":89,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DSP2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding 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