{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0DW06","uniProtkbId":"DRMB_BACP9","entryAudit":{"firstPublicDate":"2022-08-03","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2022-08-03","entryVersion":9,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Bacillus paralicheniformis (strain ATCC 9945a / NCIMB 11709 / CD-2)","taxonId":766760,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"29085076"}],"value":"DISARM protein DrmB"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"29085076"}],"value":"drmB"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AGN35228.1"}],"value":"BaLi_c08330"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29085076"}],"value":"Component of antiviral defense system DISARM (defense island system associated with restriction-modification), composed of DrmE, DrmA, DrmB, DrmC and DrmMII. DISARM is probably a multi-gene restriction module, this subunit has an unknown function. Expression of DISARM in B.subtilis (strain BEST7003) confers resistance to phages Nf, phi29, phi105, phi3T, SPO1, SPR and SPP1. Protection is over 10(7)-fold against phi3T, 10(4)-10(5)-fold against Nf, phi29, phi105 and SPR, 100-fold against SPO1 and 10-fold against SPP1. DISARM does not interfere with phage adsorption, but instead interferes with (phi3T) DNA replication early in its cycle, preventing replication, circularization and lysogeny and probably causes phage DNA degradation (DNA is degraded in SPP1-infected cells)"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P50830"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P50830"}],"value":"Binds 1 Zn(2+) per subunit."}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29085076"}],"value":"When this gene is missing the DISARM system does not confer Nf, phi3T or SPO1 resistance in B.subtilis"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":561,"modifier":"EXACT"}},"description":"DISARM protein DrmB","featureId":"PRO_0000456309"},{"type":"Binding site","location":{"start":{"value":520,"modifier":"EXACT"},"end":{"value":520,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P50830"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":522,"modifier":"EXACT"},"end":{"value":522,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P50830"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":525,"modifier":"EXACT"},"end":{"value":525,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P50830"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":533,"modifier":"EXACT"},"end":{"value":533,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P50830"},{"evidenceCode":"ECO:0000305"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}}],"keywords":[{"id":"KW-0051","category":"Biological process","name":"Antiviral defense"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0680","category":"Biological process","name":"Restriction system"},{"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"23908277","citationType":"journal article","authors":["Rachinger M.","Volland S.","Meinhardt F.","Daniel R.","Liesegang H."],"citationCrossReferences":[{"database":"PubMed","id":"23908277"},{"database":"DOI","id":"10.1128/genomea.00525-13"}],"title":"First Insights into the Completely Annotated Genome Sequence of Bacillus licheniformis Strain 9945A.","publicationDate":"2013","journal":"Genome Announc.","firstPage":"0","lastPage":"0","volume":"1"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 9945a / NCIMB 11709 / CD-2","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"29085076","citationType":"journal article","authors":["Ofir G.","Melamed S.","Sberro H.","Mukamel Z.","Silverman S.","Yaakov G.","Doron S.","Sorek R."],"citationCrossReferences":[{"database":"PubMed","id":"29085076"},{"database":"DOI","id":"10.1038/s41564-017-0051-0"}],"title":"DISARM is a widespread bacterial defence system with broad anti-phage activities.","publicationDate":"2018","journal":"Nat. Microbiol.","firstPage":"90","lastPage":"98","volume":"3"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE","EXPRESSION IN B.SUBTILIS"],"referenceComments":[{"value":"ATCC 9945a / NCIMB 11709 / CD-2","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP005965","properties":[{"key":"ProteinId","value":"AGN35228.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"P0DW06","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0DW06","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"blh:BaLi_c08330","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"InterPro","id":"IPR047721","properties":[{"key":"EntryName","value":"DrmB"}]},{"database":"InterPro","id":"IPR061785","properties":[{"key":"EntryName","value":"DUF8724"}]},{"database":"InterPro","id":"IPR018973","properties":[{"key":"EntryName","value":"MZB"}]},{"database":"NCBIfam","id":"NF038324","properties":[{"key":"EntryName","value":"DrmB_fam"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF30764","properties":[{"key":"EntryName","value":"DUF8724"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF09369","properties":[{"key":"EntryName","value":"MZB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDSVNDSLVILDIKYWTNHNNKKIFDKNLMKFLKKDYFRKIPSTKHKDLPAIPFPNYHVCSNVKCSRLFDLRDNFVMSDYLKGGPKCPDCSRKSYPARFVVSCQENHLDDFPWRWWAHGKQDTECKGKLRLWSTGNTSSLDSLYVECECKKKKSLRGAMQDSSFENYKCTGNHPHKLNEKSNCNEPVIPLQRGASNVYFPALRSAIVIPSNASEDNNLDDFFTSVKEVINTYADLIGENWHYKFYSDRLTGHSEFSDAEDFIAKWTSYLNSNDSEEEIEYNQIKEAEYRAFTAFDRKVKMGDFEAEVETVPDDFQPYFNRIVKAHRLKEILVLLGFMRNDSPEPDVNEPKKIVWLESDGYENWLPAIEVHGEGIFIEFNHTTISKWLEENSELPKRSEKFSSLYASWIESKGWEVRDEKDIVYVMLHTFAHLLIKQLSLQSGYSSVAIKERIYCGKNMAGVLLYTGSTDQEGSLGGLVEMGSIDKLRPLIVDALEEAIFCSNDPSCASLEPSEDNQLNGCACFACSMVAETSCETGNRLLDRSLLVKTIDSKYSPFFKGLL","length":561,"molWeight":64343,"crc64":"82AC677A6DE14B66","md5":"83494D19898E7E1D95CB644EE9CA6EAE"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Chain":1,"Binding site":4},"uniParcId":"UPI0003422A15"}}