{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0DW03","uniProtkbId":"ZORE_ECOLC","entryAudit":{"firstPublicDate":"2022-08-03","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2022-08-03","entryVersion":10,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Escherichia coli (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks)","taxonId":481805,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"4: Predicted","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"29371424"}],"value":"Zorya protein ZorE"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"29371424"}],"value":"Putative nuclease ZorE"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"29371424"}],"value":"zorE"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"ACA79492.1"}],"value":"EcolC_3890"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29371424"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"29371424"}],"value":"Component of antiviral defense system Zorya type II, composed of ZorA, ZorB and ZorE. Expression of Zorya type II in E.coli (strain MG1655) confers resistance to phages SECphi7 and T7. While most T7 infected Zorya-containing cells undergo abortive infection, a minority produce viable phage progeny. These eventually accumulate to a high multiplicity of infection, leading to culture collapse by 170 minutes after initial infection (PubMed:29371424). ZorA and ZorB probably assemble in the cell inner membrane and exert their effect there. This may be a nuclease (Probable)"}],"commentType":"FUNCTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":367,"modifier":"EXACT"}},"description":"Zorya protein ZorE","featureId":"PRO_0000456343"}],"keywords":[{"id":"KW-0051","category":"Biological process","name":"Antiviral defense"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-3M3PCFIDVR34C","citationType":"submission","authors":["Copeland A.","Lucas S.","Lapidus A.","Glavina del Rio T.","Dalin E.","Tice H.","Bruce D.","Goodwin L.","Pitluck S.","Kiss H.","Brettin T.","Detter J.C.","Han C.","Kuske C.R.","Schmutz J.","Larimer F.","Land M.","Hauser L.","Kyrpides N.","Mikhailova N.","Ingram L.","Richardson P."],"title":"Complete sequence of Escherichia coli C str. ATCC 8739.","publicationDate":"FEB-2008","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"ACA79492.1"}]},{"referenceNumber":2,"citation":{"id":"29371424","citationType":"journal article","authors":["Doron S.","Melamed S.","Ofir G.","Leavitt A.","Lopatina A.","Keren M.","Amitai G.","Sorek R."],"citationCrossReferences":[{"database":"PubMed","id":"29371424"},{"database":"DOI","id":"10.1126/science.aar4120"}],"title":"Systematic discovery of antiphage defense systems in the microbial pangenome.","publicationDate":"2018","journal":"Science","firstPage":"0","lastPage":"0","volume":"359"},"referencePositions":["FUNCTION"],"referenceComments":[{"value":"ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP000946","properties":[{"key":"ProteinId","value":"ACA79492.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_000777276.1","properties":[{"key":"NucleotideSequenceId","value":"NC_010468.1"}]},{"database":"AlphaFoldDB","id":"P0DW03","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecl:EcolC_3890","properties":[{"key":"Description","value":"-"}]},{"database":"CDD","id":"cd00085","properties":[{"key":"EntryName","value":"HNHc"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR059289","properties":[{"key":"EntryName","value":"DUF8245"}]},{"database":"InterPro","id":"IPR003615","properties":[{"key":"EntryName","value":"HNH_nuc"}]},{"database":"InterPro","id":"IPR049662","properties":[{"key":"EntryName","value":"ZorE-like_t2"}]},{"database":"NCBIfam","id":"NF041791","properties":[{"key":"EntryName","value":"anti-phage_ZorE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF26739","properties":[{"key":"EntryName","value":"DUF8245"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGKEVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRHKNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKSFSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTAKNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLLKAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLQNSEHEIIATVEAAWPGLKIAVNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL","length":367,"molWeight":42067,"crc64":"7669489C0F244123","md5":"B7537EEEFF9CEF145D96067013B5AD91"},"extraAttributes":{"countByCommentType":{"FUNCTION":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI0000F0B316"}}