{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0DUF8","secondaryAccessions":["A0A7M3S2P3"],"uniProtkbId":"BRXC_ECOHS","entryAudit":{"firstPublicDate":"2021-06-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2021-06-02","entryVersion":14,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Escherichia coli O9:H4 (strain HS)","taxonId":331112,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"30418590"}],"value":"Probable ATP-binding protein BrxC"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"30418590"}],"value":"BREX protein BrxC"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"30418590"}],"value":"brxC"},"orderedLocusNames":[{"value":"EcHS_A0338"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30418590"}],"value":"BREX systems (bacteriophage exclusion) provide immunity against bacteriophage. Part of a type 1 BREX system which protects against dsDNA phage. This system allows phage adsorption but prevents phage DNA replication, without degradation of the phage DNA. Methylation of bacterial DNA by PglX guides self/non-self discrimination. When the brxA-brxB-brxC-pglX-pglZ-brxL genes are transformed into a susceptible E.coli strain (BW25113) they confer very high resistance to infection by bacteriophage VR7 and VpaE1, about 100-fold protection against lambda, T5 and T7 and no protection against RNA phage Qbeta, ssDNA phage M13 or dSDNA phage T4 and VR5. Glycosylated phage DNA is not susceptible to BREX. The BREX system does not confer resistance to lysogenic lambda phage, i.e. prophage that are integrated into the chromosomal DNA and then induced to form phage"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30418590"}],"value":"Transcribed at slowly increasing levels as cells progress from lag to exponential to stationary phase"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30418590"}],"value":"No methylation of 5'-GGTAAG-3' in chromosomal DNA, BREX no longer confers phage resistance"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the BrxC family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1213,"modifier":"EXACT"}},"description":"Probable ATP-binding protein BrxC","featureId":"PRO_0000452158"}],"keywords":[{"id":"KW-0051","category":"Biological process","name":"Antiviral defense"}],"references":[{"referenceNumber":1,"citation":{"id":"18676672","citationType":"journal article","authors":["Rasko D.A.","Rosovitz M.J.","Myers G.S.A.","Mongodin E.F.","Fricke W.F.","Gajer P.","Crabtree J.","Sebaihia M.","Thomson N.R.","Chaudhuri R.","Henderson I.R.","Sperandio V.","Ravel J."],"citationCrossReferences":[{"database":"PubMed","id":"18676672"},{"database":"DOI","id":"10.1128/jb.00619-08"}],"title":"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates.","publicationDate":"2008","journal":"J. Bacteriol.","firstPage":"6881","lastPage":"6893","volume":"190"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"HS","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"30418590","citationType":"journal article","authors":["Gordeeva J.","Morozova N.","Sierro N.","Isaev A.","Sinkunas T.","Tsvetkova K.","Matlashov M.","Truncaite L.","Morgan R.D.","Ivanov N.V.","Siksnys V.","Zeng L.","Severinov K."],"citationCrossReferences":[{"database":"PubMed","id":"30418590"},{"database":"DOI","id":"10.1093/nar/gky1125"}],"title":"BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site.","publicationDate":"2019","journal":"Nucleic Acids Res.","firstPage":"253","lastPage":"265","volume":"47"},"referencePositions":["FUNCTION IN ANTIVIRAL DEFENSE","INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"HS","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP000802","properties":[{"key":"ProteinId","value":"ABV04726.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_001019654.1","properties":[{"key":"NucleotideSequenceId","value":"NC_009800.1"}]},{"database":"AlphaFoldDB","id":"P0DUF8","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecx:EcHS_A0338","properties":[{"key":"Description","value":"-"}]},{"database":"InterPro","id":"IPR050163","properties":[{"key":"EntryName","value":"Apolipoprotein_A1/A4/E"}]},{"database":"InterPro","id":"IPR047679","properties":[{"key":"EntryName","value":"BREX_BrxC"}]},{"database":"InterPro","id":"IPR058036","properties":[{"key":"EntryName","value":"BREX_BrxC_4th"}]},{"database":"InterPro","id":"IPR058037","properties":[{"key":"EntryName","value":"BREX_BrxC_helical"}]},{"database":"InterPro","id":"IPR058038","properties":[{"key":"EntryName","value":"BREX_BrxC_wHTH"}]},{"database":"InterPro","id":"IPR045725","properties":[{"key":"EntryName","value":"BrxC/PglY_N"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"NCBIfam","id":"NF033441","properties":[{"key":"EntryName","value":"BREX_BrxC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR18976","properties":[{"key":"EntryName","value":"APOLIPOPROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR18976:SF34","properties":[{"key":"EntryName","value":"LIPID-BINDING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF25796","properties":[{"key":"EntryName","value":"BREX_BrxC_4th"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF25792","properties":[{"key":"EntryName","value":"BREX_BrxC_helical"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF19557","properties":[{"key":"EntryName","value":"BrxC-PglY_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF25791","properties":[{"key":"EntryName","value":"WHD_BREX_BrxC"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNIEQIFKKPLKRNINGVVKAEQTDDASAYIELDEYVITRELENHLRHFFESYVPATGPERIRMENKIGVWVSGFFGSGKSHFIKILSYLLSNRKVTHNGTERNAYSFFEEKIKDALFLADINKAVHYPTEVILFNIDSRANVDDKEDAILKVFLKVFNERIGYCADFPHIAHLERELDKRGQYETFKAAFADINGSRWEDERDAYYFISDDMAQALSQATQQSLEASRQWVEQLDKNFPLDINNFCQWVKEWLDDNGKNILFMVDEVGQFIGKNTQMMLKLQTITENLGVICGGRAWVIVTSQADINAAIGGMSSRDGQDFSKIQGRFSTRLQLSSSNTSEVIQKRLLVKTDEAKAALAKVWQEKGDILRNQLAFDTTTTTALRPFTSEEEFVDNYPFVPWHYQILQKVFESIRTKGAAGKQLAMGERSQLEAFQTAAQQISAQGLDSLVPFWRFYAAIESFLEPAVSRTITQACQNGILDEFDGNLLKTLFLIRYVETLKSTLDNLVTLSIDRIDADKVELRRRVEKSLNTLERLMLIARVEDKYVFLTNEEKEIENEIRNVDVDFSAINKKLASIIFDDILKSRKYRYPANKQDFDISRFLNGHPLDGAVLNDLVVKILTPKDPTYSFYNSDATCRPYTSEGDGCILIRLPEEGRTWSDIDLVVQTEKFLKDNAGQRPEQATLLSEKARENSNREKLLRVQLESLLAEADVWAIGERLPKKSSTPSNIVDEACRYVIENTFGKLKMLRPFNGDISREIHALLTVENDTELDLGNLEESNPDAMREVETWISMNIEYNKPVYLRDILNHFARRPYGWPEDEVKLLVARLACKGKFSFSQQNNNVERKQAWELFNNSRRHSELRLHKVRRHDEAQVRKAAQTMADIAQQPFNEREEPALVEHIRQVFEEWKQELNVFRAKAEGGNNPGKNEIESGLRLLNAILNEKEDFALIEKVSSLKDELLDFSEDREDLVDFYRKQFATWQKLGAALNGSFKSNRSALEKDAAAVKALGELESIWQMPEPYKHLNRITLLIEQVQNVNHQLVEQHRQHALERIDARIEESRQRLLEAHATSELQNSVLLPMQKARKRAEVSQSIPEILAEQQETKALQMDADKKINLWIDELRKKQEAQLRAANEAKRAAESEQTYVVVEKPVIQPVPKKTHLVNVASEMRNATGGEVLETTEQVEKALDTLRTTLLAAIKAGDRIRLQ","length":1213,"molWeight":139272,"crc64":"D38EA50CD5612374","md5":"F345D035C332B2BEBAE3D9C4C8AA1488"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI00005F13E3"}}