{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0CJ98","uniProtkbId":"MVP_PRSVH","entryAudit":{"firstPublicDate":"2011-05-31","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2012-10-31","entryVersion":42,"sequenceVersion":2},"annotationScore":4.0,"organism":{"scientificName":"Papaya ringspot virus (strain P / mutant HA)","taxonId":31731,"lineage":["Viruses","Riboviria","Orthornavirae","Pisuviricota","Stelpaviricetes","Patatavirales","Potyviridae","Potyvirus","Potyvirus papayanuli","Papaya ringspot virus"]},"organismHosts":[{"scientificName":"Carica papaya","commonName":"Papaya","taxonId":3649}],"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"P3N-PIPO polyprotein"}},"contains":[{"recommendedName":{"fullName":{"value":"P1 protease"},"ecNumbers":[{"value":"3.4.21.-"}]},"alternativeNames":[{"fullName":{"value":"N-terminal protein"}},{"fullName":{"value":"P1 proteinase"}}]},{"recommendedName":{"fullName":{"value":"Helper component proteinase"},"shortNames":[{"value":"HC-pro"}],"ecNumbers":[{"value":"3.4.22.45"}]}},{"recommendedName":{"fullName":{"value":"Movement protein P3N-PIPO"}},"alternativeNames":[{"fullName":{"value":"Pretty interesting potyviridae ORF"},"shortNames":[{"value":"PIPO"}]}]}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P04517"}],"value":"Required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity"}],"commentType":"FUNCTION","molecule":"Helper component proteinase"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0CK11"}],"value":"Allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding"}],"commentType":"FUNCTION","molecule":"Movement protein P3N-PIPO"},{"commentType":"CATALYTIC ACTIVITY","molecule":"Helper component proteinase","reaction":{"name":"Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.","ecNumber":"3.4.22.45"}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0CK11"}],"value":"Interacts (via PIPO domain) with host PCaP1 protein; this interaction may help to anchor the movement complex to the plasma membrane from which the complex could move to the plasmodesmata"}],"commentType":"SUBUNIT","molecule":"Movement protein P3N-PIPO"},{"commentType":"SUBCELLULAR LOCATION","molecule":"Movement protein P3N-PIPO","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0CK11"}],"value":"Host cell junction, host plasmodesma","id":"SL-0385"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Ribosomal frameshifting"],"isoforms":[{"name":{"value":"P3N-PIPO polyprotein"},"isoformIds":["P0CJ98-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"Genome polyprotein"},"isoformIds":["Q01901-1"],"isoformSequenceStatus":"External"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"The N-terminus of helper component proteinase is involved in interaction with stylets. The central part is involved in interaction with virions and the C-terminus is involved in cell-to cell movement of the virus (By similarity)"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Potyviral RNA is expressed as two polyproteins which undergo post-translational proteolytic processing. Genome polyprotein is processed by NIa-pro, P1 and HC-pro proteinases resulting in the production of at least ten individual proteins. P3N-PIPO is cleaved by P1 and HC-pro proteinases resulting in the production of three individual proteins. The P1 proteinase and the HC-pro cleave only their respective C-termini autocatalytically (By similarity)"}],"commentType":"PTM"},{"texts":[{"value":"Produced by -1 ribosomal frameshifting in P3 ORF"}],"commentType":"MISCELLANEOUS","molecule":"Isoform P3N-PIPO polyprotein"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the potyviridae P3N-PIPO polyprotein family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1228,"modifier":"EXACT"}},"description":"P3N-PIPO polyprotein","featureId":"PRO_0000420060"},{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":547,"modifier":"EXACT"}},"description":"P1 protease","evidences":[{"evidenceCode":"ECO:0000250"}],"featureId":"PRO_0000420061"},{"type":"Chain","location":{"start":{"value":548,"modifier":"EXACT"},"end":{"value":1004,"modifier":"EXACT"}},"description":"Helper component proteinase","evidences":[{"evidenceCode":"ECO:0000250"}],"featureId":"PRO_0000420062"},{"type":"Chain","location":{"start":{"value":1005,"modifier":"EXACT"},"end":{"value":1228,"modifier":"EXACT"}},"description":"Movement protein P3N-PIPO","featureId":"PRO_0000408542"},{"type":"Domain","location":{"start":{"value":408,"modifier":"EXACT"},"end":{"value":547,"modifier":"EXACT"}},"description":"Peptidase S30","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01219"}]},{"type":"Domain","location":{"start":{"value":882,"modifier":"EXACT"},"end":{"value":1004,"modifier":"EXACT"}},"description":"Peptidase C6","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01080"}]},{"type":"Motif","location":{"start":{"value":598,"modifier":"EXACT"},"end":{"value":601,"modifier":"EXACT"}},"description":"Involved in interaction with stylet and aphid transmission","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Motif","location":{"start":{"value":856,"modifier":"EXACT"},"end":{"value":858,"modifier":"EXACT"}},"description":"Involved in virions binding and aphid transmission","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Active site","location":{"start":{"value":456,"modifier":"EXACT"},"end":{"value":456,"modifier":"EXACT"}},"description":"For P1 proteinase activity","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01219"}]},{"type":"Active site","location":{"start":{"value":465,"modifier":"EXACT"},"end":{"value":465,"modifier":"EXACT"}},"description":"For P1 proteinase activity","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01219"}]},{"type":"Active site","location":{"start":{"value":499,"modifier":"EXACT"},"end":{"value":499,"modifier":"EXACT"}},"description":"For P1 proteinase activity","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01219"}]},{"type":"Active site","location":{"start":{"value":890,"modifier":"EXACT"},"end":{"value":890,"modifier":"EXACT"}},"description":"For helper component proteinase activity","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01080"}]},{"type":"Active site","location":{"start":{"value":963,"modifier":"EXACT"},"end":{"value":963,"modifier":"EXACT"}},"description":"For helper component proteinase activity","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01080"}]},{"type":"Site","location":{"start":{"value":547,"modifier":"EXACT"},"end":{"value":548,"modifier":"EXACT"}},"description":"Cleavage; by P1 proteinase","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01219"}]},{"type":"Site","location":{"start":{"value":1004,"modifier":"EXACT"},"end":{"value":1005,"modifier":"EXACT"}},"description":"Cleavage; by autolysis","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01080"}]},{"type":"Sequence uncertainty","location":{"start":{"value":1155,"modifier":"EXACT"},"end":{"value":1161,"modifier":"EXACT"}},"description":""}],"keywords":[{"id":"KW-1031","category":"Cellular component","name":"Host cell junction"},{"id":"KW-0945","category":"Biological process","name":"Host-virus interaction"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-1090","category":"Biological process","name":"Inhibition of host innate immune response by virus"},{"id":"KW-0645","category":"Molecular function","name":"Protease"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0688","category":"Coding sequence diversity","name":"Ribosomal frameshifting"},{"id":"KW-0720","category":"Molecular function","name":"Serine protease"},{"id":"KW-0941","category":"Biological process","name":"Suppressor of RNA silencing"},{"id":"KW-0813","category":"Biological process","name":"Transport"},{"id":"KW-0899","category":"Biological process","name":"Viral immunoevasion"},{"id":"KW-0916","category":"Molecular function","name":"Viral movement protein"}],"references":[{"referenceNumber":1,"citation":{"id":"IND20450567","citationType":"journal article","authors":["Wang C.H.","Bau H.J.","Yeh S.D."],"citationCrossReferences":[{"database":"AGRICOLA","id":"IND20450567"}],"title":"Comparison of the nuclear inclusion b protein and coat protein genes of five papaya ringspot virus strains distinct in geographic origin and pathogenicity.","publicationDate":"1994","journal":"Phytopathology","firstPage":"1205","lastPage":"1210","volume":"84"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC RNA]"]},{"referenceNumber":2,"citation":{"id":"1402799","citationType":"journal article","authors":["Yeh S.D.","Jan F.J.","Chiang C.H.","Doong T.J.","Chen M.C.","Chung P.H.","Bau H.J."],"citationCrossReferences":[{"database":"PubMed","id":"1402799"},{"database":"DOI","id":"10.1099/0022-1317-73-10-2531"}],"title":"Complete nucleotide sequence and genetic organization of papaya ringspot virus RNA.","publicationDate":"1992","journal":"J. Gen. Virol.","firstPage":"2531","lastPage":"2541","volume":"73"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC RNA]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X67673","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_RNA"}]},{"database":"EMBL","id":"S46722","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_RNA"}]},{"database":"SMR","id":"P0CJ98","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006688","properties":[{"key":"Component","value":"Segment"}]},{"database":"GO","id":"GO:0044219","properties":[{"key":"GoTerm","value":"C:host cell plasmodesma"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0004197","properties":[{"key":"GoTerm","value":"F:cysteine-type endopeptidase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008236","properties":[{"key":"GoTerm","value":"F:serine-type peptidase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0006508","properties":[{"key":"GoTerm","value":"P:proteolysis"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0052170","properties":[{"key":"GoTerm","value":"P:symbiont-mediated suppression of host innate immune response"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0046740","properties":[{"key":"GoTerm","value":"P:transport of virus in host, cell to cell"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0075523","properties":[{"key":"GoTerm","value":"P:viral translational frameshifting"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"Gene3D","id":"3.90.70.150","properties":[{"key":"EntryName","value":"Helper component proteinase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR022199","properties":[{"key":"EntryName","value":"DUF3725"}]},{"database":"InterPro","id":"IPR001456","properties":[{"key":"EntryName","value":"HC-pro"}]},{"database":"InterPro","id":"IPR031159","properties":[{"key":"EntryName","value":"HC_PRO_CPD_dom"}]},{"database":"InterPro","id":"IPR042308","properties":[{"key":"EntryName","value":"HC_PRO_CPD_sf"}]},{"database":"InterPro","id":"IPR002540","properties":[{"key":"EntryName","value":"Pept_S30_P1_potyvir"}]},{"database":"InterPro","id":"IPR039560","properties":[{"key":"EntryName","value":"Potyvirid-P3"}]},{"database":"Pfam","id":"PF12523","properties":[{"key":"EntryName","value":"DUF3725"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00851","properties":[{"key":"EntryName","value":"Peptidase_C6"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01577","properties":[{"key":"EntryName","value":"Peptidase_S30"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13608","properties":[{"key":"EntryName","value":"Potyvirid-P3"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51744","properties":[{"key":"EntryName","value":"HC_PRO_CPD"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51871","properties":[{"key":"EntryName","value":"PV_P1_PRO"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSSLYTLRAAAQYDRRLESKKGSGWVEHKLERKGERGNTHYCSEFDISKGAKILQLVQIGNTEVGRTFLEGNRFVRANIFEIIRKTMVGRLGYDFESELWVCRNCDKTSEKYFKKCDCGETYYYSERNLMRTMNDLMYQFDMTPSEINSVDLEYLANAVDYAEQLVKRSQVPEPVELAMMEPIVASGEGILMVSEPEVMPVTTKVEEAWTIQIGEIPVPLVVIKETPVISGVEGTLNSTGFSLEADITKLVEKEILQEEVKEAVHLALEVGNEIAEKKPELKLIPYWSASLELHKRIRKHKEHAKIAAIQVQKEREKDQKVFSALELRLNLKSRRRNQAVVCDKRGTLKWETQRGHKKSKLMQQASDFVVTQIHCDFGCKTQYSEPHIPGIKQSTSKKICKPRKHSRIVGNSKINYIMKNLCDTIIERGIPVELVTKRCKRRILQKEGRSYVQLRHMNGIRARQDVSSSPDMELLFTQFCKFLVGHKPLKSKNLTFGSSGLIFKPKFADNVGRYFGDYFVVRGRLGGKLFDGRSKLARSVYAKMDQYNDVAEKFWLGFNRAFLRHRKPTDHTCTSDMDVTMCGEVAALATIILFPCHKITCNTCMSKVKGRVIDEVGEDLNCELERLRETLSAYGGSFGHVSTLLDQLNRVLNARNMNDGAFKEIAKKIDEKKESPWTHMTTINNTLYKGSLATGYEFERASNSLREIVRWHLKRTESIKAGSVESFRNKRSGKAHFNPALTCDNQLDKNGNFLWGERQYHAKRFFANYFEKIDHSKGYEYYSQRQNPNGIRKIAIGNLVFSTNLERFRQQMVEHHIDQGPITRECIALRNNNYVHVCSCVTLDDGTPATSELKTPTKNHIVLGNSGDPKYVDLPTLESDSMYIAKKGYCYMNIFLAMLINIPENEAKDFTKRVRDLVGSKLGEWPTMLDVATCANQLVVFHPDAANAELPQILVDHRQKTMHVIDSFGSVDSGYHILKANTVNQLIQFARDPLDSEMKHYIVGGEFDPTTNCLHQLIRVIYKPHELRSLLRNEPYLIVIALMSPSVLLTLFNSGAVEHALNYWIKRDQDVVEVIVLVEQLCRKVTLARTILEQFNEIRQNARDLHELMDRNNKPWISYDRSLELLSVYANSQLTDEGLLKQGFSTLDPRLREAVEKNLRHSFAGRMACVKFVSKVALKVLCVQITTVFFRVFKAKRARRFKNCIRLLTEILCTRGRKSVPTASQGWG","length":1228,"molWeight":140607,"crc64":"AC25362634FE64A4","md5":"C1664981020940EABD3391258A8E709F"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":2,"DOMAIN":1,"PTM":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":4,"Domain":2,"Motif":2,"Active site":5,"Site":2,"Sequence uncertainty":1},"uniParcId":"UPI000287A2AB"}}