{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0C9U3","uniProtkbId":"5057R_ASFM2","entryAudit":{"firstPublicDate":"2009-05-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2009-05-05","entryVersion":41,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983)","commonName":"ASFV","taxonId":10500,"lineage":["Viruses","Varidnaviria","Bamfordvirae","Nucleocytoviricota","Pokkesviricetes","Asfuvirales","Asfarviridae","Asfivirus","Asfivirus haemorrhagiae","African swine fever virus"]},"organismHosts":[{"scientificName":"Ornithodoros","commonName":"relapsing fever ticks","taxonId":6937},{"scientificName":"Phacochoerus aethiopicus","commonName":"Warthog","taxonId":85517},{"scientificName":"Phacochoerus africanus","commonName":"Warthog","taxonId":41426},{"scientificName":"Potamochoerus larvatus","commonName":"Bushpig","taxonId":273792},{"scientificName":"Sus scrofa","commonName":"Pig","taxonId":9823}],"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Protein MGF 505-7R"}}},"genes":[{"orderedLocusNames":[{"value":"Mal-039"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q89925"}],"value":"Plays a role in virus cell tropism, and may be required for efficient virus replication in macrophages. Interferes with host NF-kappa-B promoter activity mediated by TLR8. Mechanistically, inhibits the phosphorylation and subsequent nuclear translocation of host NF-kappa-B RELA subunit downstream of TLR8. Promotes the expression of the autophagy-related protein host ULK1 to degrade host STING and inhibit the interferon response. Also inhibits JAK1- and JAK2-mediated signaling and thus negatively regulates the IFN-gamma signaling"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q89925"}],"value":"Interacts with host STING1. Interacts with host JAK1; this interaction leads to JAK1 degradation. Interacts with host JAK2; this interaction leads to JAK2 degradation. Interacts with host RELA; this interaction inhibits NF-kappa-B promoter activity"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q89925"}],"value":"Host cytoplasm","id":"SL-0381"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q89925"}],"value":"Expressed in the early phase of the viral replicative cycle"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the asfivirus MGF 505 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":528,"modifier":"EXACT"}},"description":"Protein MGF 505-7R","featureId":"PRO_0000373341"},{"type":"Repeat","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"ANK 1"},{"type":"Repeat","location":{"start":{"value":261,"modifier":"EXACT"},"end":{"value":291,"modifier":"EXACT"}},"description":"ANK 2"}],"keywords":[{"id":"KW-0040","category":"Domain","name":"ANK repeat"},{"id":"KW-0244","category":"Developmental stage","name":"Early protein"},{"id":"KW-1035","category":"Cellular component","name":"Host cytoplasm"},{"id":"KW-0945","category":"Biological process","name":"Host-virus interaction"},{"id":"KW-1090","category":"Biological process","name":"Inhibition of host innate immune response by virus"},{"id":"KW-1114","category":"Biological process","name":"Inhibition of host interferon signaling pathway by virus"},{"id":"KW-1096","category":"Biological process","name":"Inhibition of host JAK1 by virus"},{"id":"KW-0922","category":"Biological process","name":"Interferon antiviral system evasion"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0899","category":"Biological process","name":"Viral immunoevasion"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-780ILRT06KVP4","citationType":"submission","authors":["Kutish G.F.","Rock D.L."],"title":"African swine fever virus genomes.","publicationDate":"MAR-2003","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY261361","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"SMR","id":"P0C9U3","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000860","properties":[{"key":"Component","value":"Segment"}]},{"database":"GO","id":"GO:0030430","properties":[{"key":"GoTerm","value":"C:host cell cytoplasm"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0052170","properties":[{"key":"GoTerm","value":"P:symbiont-mediated suppression of host innate immune response"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0039576","properties":[{"key":"GoTerm","value":"P:symbiont-mediated suppression of host JAK-STAT cascade via inhibition of JAK1 activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0039502","properties":[{"key":"GoTerm","value":"P:symbiont-mediated suppression of host type I interferon-mediated signaling pathway"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"Gene3D","id":"1.25.40.20","properties":[{"key":"EntryName","value":"Ankyrin repeat-containing domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036770","properties":[{"key":"EntryName","value":"Ankyrin_rpt-contain_sf"}]},{"database":"InterPro","id":"IPR004858","properties":[{"key":"EntryName","value":"MGF_505"}]},{"database":"Pfam","id":"PF03158","properties":[{"key":"EntryName","value":"DUF249"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48403","properties":[{"key":"EntryName","value":"Ankyrin repeat"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFSLQDLCRKNTFFLPNGFNKHTLQLLGLYWKEHGSVHRVEKDNIMIQNELVLSINDALLLAGEEGDTDVVQLLLLWEGNLHYAIIGALKTEKYNLICEYHSQIEDWHILLPMIQDPETFEKCHDLSLQCDFICLLQHAIKYNKLSILVKYKEDLLNIRIRHRVQSLFVLACENRRTEIIEWIGQNLSIPEPDAIFSIAIATKDLELFSLGYKIIFDYMQRQGILQLSNGLRMVVLNRHISMAIDNGLLPFVLETLKHGGNIHRALSYAVSHNRRKILDYLIRQKNIPPNTIERLLYLAVKNQSSRKTLNLLLSYINYKVKNVKKLVEHVVDHNSTLVVTILLEKKINLIDAVLTRLVKHSTYFRVREFIQEFSISPEKFIKIAVREKRNVIIKGISEDIWENPAERITYLKQIVHTIKYESGREFLINIIHTIYQSYSLKPEEILKLARFYVKHNATTHFKDLCKYLWLNRGTESKKLFLECLEIADKKDFPAIKNIVSEYINYLFTAGAITKEEIMQVYTLEYAMY","length":528,"molWeight":61838,"crc64":"B806D2C74D5992A5","md5":"CCB513DA9ED8AE952C998B4BD8CB316A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Repeat":2},"uniParcId":"UPI00019B22CC"}}