{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0C874","uniProtkbId":"S31D3_HUMAN","entryAudit":{"firstPublicDate":"2008-09-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2008-09-02","entryVersion":94,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Spermatogenesis-associated protein 31D3"}},"alternativeNames":[{"fullName":{"value":"Protein FAM75D3"}}]},"genes":[{"geneName":{"value":"SPATA31D3"},"synonyms":[{"value":"FAM75D3"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"May play a role in spermatogenesis"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Membrane","id":"SL-0162"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Single-pass membrane protein","id":"SL-9904"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the SPATA31 family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"AK126070","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"AK308327","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":917,"modifier":"EXACT"}},"description":"Spermatogenesis-associated protein 31D3","featureId":"PRO_0000349241"},{"type":"Transmembrane","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":49,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Region","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":80,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":195,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":773,"modifier":"EXACT"},"end":{"value":797,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"Basic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":164,"modifier":"EXACT"},"end":{"value":177,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":782,"modifier":"EXACT"},"end":{"value":797,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Sequence conflict","location":{"start":{"value":389,"modifier":"EXACT"},"end":{"value":389,"modifier":"EXACT"}},"description":"in Ref. 1; AK308327","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["R"]}},{"type":"Sequence conflict","location":{"start":{"value":541,"modifier":"EXACT"},"end":{"value":541,"modifier":"EXACT"}},"description":"in Ref. 1; AK308327","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["F"]}}],"keywords":[{"id":"KW-0221","category":"Biological process","name":"Differentiation"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0744","category":"Biological process","name":"Spermatogenesis"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"14702039","citationType":"journal article","authors":["Ota T.","Suzuki Y.","Nishikawa T.","Otsuki T.","Sugiyama T.","Irie R.","Wakamatsu A.","Hayashi K.","Sato H.","Nagai K.","Kimura K.","Makita H.","Sekine M.","Obayashi M.","Nishi T.","Shibahara T.","Tanaka T.","Ishii S.","Yamamoto J.","Saito K.","Kawai Y.","Isono Y.","Nakamura Y.","Nagahari K.","Murakami K.","Yasuda T.","Iwayanagi T.","Wagatsuma M.","Shiratori A.","Sudo H.","Hosoiri T.","Kaku Y.","Kodaira H.","Kondo H.","Sugawara M.","Takahashi M.","Kanda K.","Yokoi T.","Furuya T.","Kikkawa E.","Omura Y.","Abe K.","Kamihara K.","Katsuta N.","Sato K.","Tanikawa M.","Yamazaki M.","Ninomiya K.","Ishibashi T.","Yamashita H.","Murakawa K.","Fujimori K.","Tanai H.","Kimata M.","Watanabe M.","Hiraoka S.","Chiba Y.","Ishida S.","Ono Y.","Takiguchi S.","Watanabe S.","Yosida M.","Hotuta T.","Kusano J.","Kanehori K.","Takahashi-Fujii A.","Hara H.","Tanase T.-O.","Nomura Y.","Togiya S.","Komai F.","Hara R.","Takeuchi K.","Arita M.","Imose N.","Musashino K.","Yuuki H.","Oshima A.","Sasaki N.","Aotsuka S.","Yoshikawa Y.","Matsunawa H.","Ichihara T.","Shiohata N.","Sano S.","Moriya S.","Momiyama H.","Satoh N.","Takami S.","Terashima Y.","Suzuki O.","Nakagawa S.","Senoh A.","Mizoguchi H.","Goto Y.","Shimizu F.","Wakebe H.","Hishigaki H.","Watanabe T.","Sugiyama A.","Takemoto M.","Kawakami B.","Yamazaki M.","Watanabe K.","Kumagai A.","Itakura S.","Fukuzumi Y.","Fujimori Y.","Komiyama M.","Tashiro H.","Tanigami A.","Fujiwara T.","Ono T.","Yamada K.","Fujii Y.","Ozaki K.","Hirao M.","Ohmori Y.","Kawabata A.","Hikiji T.","Kobatake N.","Inagaki H.","Ikema Y.","Okamoto S.","Okitani R.","Kawakami T.","Noguchi S.","Itoh T.","Shigeta K.","Senba T.","Matsumura K.","Nakajima Y.","Mizuno T.","Morinaga M.","Sasaki M.","Togashi T.","Oyama M.","Hata H.","Watanabe M.","Komatsu T.","Mizushima-Sugano J.","Satoh T.","Shirai Y.","Takahashi Y.","Nakagawa K.","Okumura K.","Nagase T.","Nomura N.","Kikuchi H.","Masuho Y.","Yamashita R.","Nakai K.","Yada T.","Nakamura Y.","Ohara O.","Isogai T.","Sugano S."],"citationCrossReferences":[{"database":"PubMed","id":"14702039"},{"database":"DOI","id":"10.1038/ng1285"}],"title":"Complete sequencing and characterization of 21,243 full-length human cDNAs.","publicationDate":"2004","journal":"Nat. Genet.","firstPage":"40","lastPage":"45","volume":"36"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"15164053","citationType":"journal article","authors":["Humphray S.J.","Oliver K.","Hunt A.R.","Plumb R.W.","Loveland J.E.","Howe K.L.","Andrews T.D.","Searle S.","Hunt S.E.","Scott C.E.","Jones M.C.","Ainscough R.","Almeida J.P.","Ambrose K.D.","Ashwell R.I.S.","Babbage A.K.","Babbage S.","Bagguley C.L.","Bailey J.","Banerjee R.","Barker D.J.","Barlow K.F.","Bates K.","Beasley H.","Beasley O.","Bird C.P.","Bray-Allen S.","Brown A.J.","Brown J.Y.","Burford D.","Burrill W.","Burton J.","Carder C.","Carter N.P.","Chapman J.C.","Chen Y.","Clarke G.","Clark S.Y.","Clee C.M.","Clegg S.","Collier R.E.","Corby N.","Crosier M.","Cummings A.T.","Davies J.","Dhami P.","Dunn M.","Dutta I.","Dyer L.W.","Earthrowl M.E.","Faulkner L.","Fleming C.J.","Frankish A.","Frankland J.A.","French L.","Fricker D.G.","Garner P.","Garnett J.","Ghori J.","Gilbert J.G.R.","Glison C.","Grafham D.V.","Gribble S.","Griffiths C.","Griffiths-Jones S.","Grocock R.","Guy J.","Hall R.E.","Hammond S.","Harley J.L.","Harrison E.S.I.","Hart E.A.","Heath P.D.","Henderson C.D.","Hopkins B.L.","Howard P.J.","Howden P.J.","Huckle E.","Johnson C.","Johnson D.","Joy A.A.","Kay M.","Keenan S.","Kershaw J.K.","Kimberley A.M.","King A.","Knights A.","Laird G.K.","Langford C.","Lawlor S.","Leongamornlert D.A.","Leversha M.","Lloyd C.","Lloyd D.M.","Lovell J.","Martin S.","Mashreghi-Mohammadi M.","Matthews L.","McLaren S.","McLay K.E.","McMurray A.","Milne S.","Nickerson T.","Nisbett J.","Nordsiek G.","Pearce A.V.","Peck A.I.","Porter K.M.","Pandian R.","Pelan S.","Phillimore B.","Povey S.","Ramsey Y.","Rand V.","Scharfe M.","Sehra H.K.","Shownkeen R.","Sims S.K.","Skuce C.D.","Smith M.","Steward C.A.","Swarbreck D.","Sycamore N.","Tester J.","Thorpe A.","Tracey A.","Tromans A.","Thomas D.W.","Wall M.","Wallis J.M.","West A.P.","Whitehead S.L.","Willey D.L.","Williams S.A.","Wilming L.","Wray P.W.","Young L.","Ashurst J.L.","Coulson A.","Blocker H.","Durbin R.M.","Sulston J.E.","Hubbard T.","Jackson M.J.","Bentley D.R.","Beck S.","Rogers J.","Dunham I."],"citationCrossReferences":[{"database":"PubMed","id":"15164053"},{"database":"DOI","id":"10.1038/nature02465"}],"title":"DNA sequence and analysis of human chromosome 9.","publicationDate":"2004","journal":"Nature","firstPage":"369","lastPage":"374","volume":"429"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AK126070","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK308327","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AL158154","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"CCDS","id":"CCDS83380.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_997299.2","properties":[{"key":"NucleotideSequenceId","value":"NM_207416.3"}]},{"database":"AlphaFoldDB","id":"P0C874","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"133259","properties":[{"key":"Interactions","value":"2"}]},{"database":"FunCoup","id":"P0C874","properties":[{"key":"Number of interactors","value":"9"}]},{"database":"IntAct","id":"P0C874","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"9606.ENSP00000488117","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"P0C874","properties":[{"key":"glycosylation","value":"2 sites, 1 O-linked glycan (1 site)"}]},{"database":"iPTMnet","id":"P0C874","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"P0C874","properties":[{"key":"Description","value":"-"}]},{"database":"BioMuta","id":"SPATA31D3","properties":[{"key":"Description","value":"-"}]},{"database":"DMDM","id":"205831115","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P0C874","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"P0C874","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"9606-ENSP00000488117","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P0C874","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"52409","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"389762","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENST00000445385.3","properties":[{"key":"ProteinId","value":"ENSP00000488117.1"},{"key":"GeneId","value":"ENSG00000186788.14"}]},{"database":"GeneID","id":"389762","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hsa:389762","properties":[{"key":"Description","value":"-"}]},{"database":"MANE-Select","id":"ENST00000445385.3","properties":[{"key":"ProteinId","value":"ENSP00000488117.1"},{"key":"RefSeqNucleotideId","value":"NM_207416.3"},{"key":"RefSeqProteinId","value":"NP_997299.2"}]},{"database":"AGR","id":"HGNC:38603","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"389762","properties":[{"key":"Description","value":"-"}]},{"database":"GeneCards","id":"SPATA31D3","properties":[{"key":"Description","value":"-"}]},{"database":"HGNC","id":"HGNC:38603","properties":[{"key":"GeneName","value":"SPATA31D3"}]},{"database":"HPA","id":"ENSG00000186788","properties":[{"key":"ExpressionPatterns","value":"Tissue enriched (testis)"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000186788","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00950000183043","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_007854_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P0C874","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"FLMCKES","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9538932at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PAN-GO","id":"P0C874","properties":[{"key":"Number of GO annotations","value":"0 GO annotations based on evolutionary models"}]},{"database":"BioGRID-ORCS","id":"389762","properties":[{"key":"hits","value":"5 hits in 217 CRISPR screens"}]},{"database":"GenomeRNAi","id":"389762","properties":[{"key":"Description","value":"-"}]},{"database":"Pharos","id":"P0C874","properties":[{"key":"DevelopmentLevel","value":"Tdark"}]},{"database":"PRO","id":"PR:P0C874","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 9"}]},{"database":"RNAct","id":"P0C874","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSG00000186788","properties":[{"key":"ExpressionPatterns","value":"Expressed in male germ line stem cell (sensu Vertebrata) in testis and 6 other cell types or tissues"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"InterPro","id":"IPR039509","properties":[{"key":"EntryName","value":"SPATA31"}]},{"database":"InterPro","id":"IPR027970","properties":[{"key":"EntryName","value":"SPATA31-like"}]},{"database":"PANTHER","id":"PTHR21859","properties":[{"key":"EntryName","value":"ACROSOME-SPECIFIC PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21859:SF50","properties":[{"key":"EntryName","value":"SPERMATOGENESIS-ASSOCIATED PROTEIN 31D3-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF15371","properties":[{"key":"EntryName","value":"DUF4599"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF14650","properties":[{"key":"EntryName","value":"FAM75"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MENILCFLNSYTETGLSPDSHCLDIDLNFICLSGLGLFILYLFYMVLTLYSSPTEKNNDTQKHQGRARRKRKSVTFKDRKSLQKEAEEERKLHSFLKSFGPPVSCSPLGQHHDTTLFRRLLCPDPVCRVCNRATADIQRLLSWESLKDAAPSVSPLASSASGAESSFTLASTPSATTPEDLILSSRPKPSPPPPLILSPDLITTLADLFSPSPLRDPLPPQPVSPLDSKFPIDHSPPQQLPFPLLPPHHIERVEPSLQPEASLSLNTIFSFGSTLCQDISQAVNRTDSCARHHGPPTPSALPPEDCTVTQSKSNLTVLKTFPEMLSLGGSGGSSTSAPTTKGIDHSCPASSEFSWWQPHAKDSFSSNFVPSDFMEELLTLHSSEASLGGHSVANIIQPVNISFLSHDIPALLERQVKRRGDFLMWKENGKKPGSFPTQLRPNYQLNSSRNMLTSTAVKHDLAESFPFWASKGKLEWQHIHQQPPYSKCFEDHLEQKYVQLFWGLPSLHSESLHPTVFVQHGRSSMFVFFNGITNTSMSHESPVLPPPQPLFLPSTQPLPLPQTLPRGQSLHLTQVKSLAQPQSPFPALPPSPLFLIRVCGVCFHRPQNEARSLMPSEINHLEWNVLQKVQESVWGLPSVVQKSQEDFCPPAPNPVLVRKSFKVHVPISIIPGDFPLSSEVRKKLEQHIRKRLIQRRWGLPRRIHESLSLLRPQNKISELSVSESIHGPLNISLVEGQRCNVLKKSASSFPRSFHERSSNMLSMENVGNYQGCSQETAPKNHLLHDPETSSEEDLRSNSERDLGTHMMHLSGNDSGVRLGQKQLENALTVHLSKKFEEINEGRMPGTVHSSWHSVKQTICLPEKSHSQIKHRNLAALVSEDHRVDTSQEMSFLSSNKQKMLEAHIKSFHMKPILNLSI","length":917,"molWeight":102419,"crc64":"DB9FB7B800EA3322","md5":"DB9D13728A1257ACC1798D5BCF2756E1"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"SEQUENCE CAUTION":2},"countByFeatureType":{"Chain":1,"Transmembrane":1,"Region":3,"Compositional bias":4,"Sequence conflict":2},"uniParcId":"UPI000048D678"}}