{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0AGI1","secondaryAccessions":["P04984","Q2M868"],"uniProtkbId":"RBSC_ECOLI","entryAudit":{"firstPublicDate":"1987-08-13","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-12-20","entryVersion":139,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Ribose import permease protein RbsC"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"6327617"}],"value":"rbsC"},"orderedLocusNames":[{"value":"b3750"},{"value":"JW3729"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25533465"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"6327617"}],"value":"Part of the ABC transporter complex RbsABC involved in ribose import. Probably responsible for the translocation of the substrate across the membrane"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26305955"}],"value":"(Microbial infection) Probably transports the toxic C-terminal region of CdiA from D.dadantii strain 3937 across the inner membrane to the cytoplasm, where CdiA has a toxic effect. Toxin transport is strain-specific, mutations in this gene do not confer resistance to several other tested CdiA toxins"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25533465"}],"value":"The complex is composed of an ATP-binding protein (RbsA), two transmembrane proteins (RbsC) and a solute-binding protein (RbsB)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P0AGI1","intActId":"EBI-21444614"},"interactantTwo":{"uniProtKBAccession":"P04983","geneName":"rbsA","intActId":"EBI-1132449"},"numberOfExperiments":5,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P0AGI1","intActId":"EBI-21444614"},"interactantTwo":{"uniProtKBAccession":"P02925","geneName":"rbsB","intActId":"EBI-369930"},"numberOfExperiments":6,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15919996"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9922273"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9922273"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26305955"}],"value":"Disruption confers resistance to cellular contact-dependent growth inhibition (CDI) CdiA of D.dadantii strain 3937, but not to several other tested CdiA toxins"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the binding protein-dependent transport system permease family. AraH/RbsC subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":321,"modifier":"EXACT"}},"description":"Ribose import permease protein RbsC","featureId":"PRO_0000060223"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":22,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9922273"}]},{"type":"Transmembrane","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9922273"}]},{"type":"Topological domain","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9922273"}]},{"type":"Transmembrane","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9922273"}]},{"type":"Topological domain","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":125,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9922273"}]},{"type":"Transmembrane","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":145,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9922273"}]},{"type":"Topological domain","location":{"start":{"value":146,"modifier":"EXACT"},"end":{"value":168,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9922273"}]},{"type":"Transmembrane","location":{"start":{"value":169,"modifier":"EXACT"},"end":{"value":190,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9922273"}]},{"type":"Topological domain","location":{"start":{"value":191,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9922273"}]},{"type":"Transmembrane","location":{"start":{"value":221,"modifier":"EXACT"},"end":{"value":240,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9922273"}]},{"type":"Topological domain","location":{"start":{"value":241,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9922273"}]},{"type":"Transmembrane","location":{"start":{"value":295,"modifier":"EXACT"},"end":{"value":316,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9922273"}]},{"type":"Topological domain","location":{"start":{"value":317,"modifier":"EXACT"},"end":{"value":321,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15919996"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9922273"}]},{"type":"Sequence conflict","location":{"start":{"value":202,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"in Ref. 1; AAA51474","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["V"]}}],"keywords":[{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0762","category":"Biological process","name":"Sugar transport"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"3011793","citationType":"journal article","authors":["Bell A.W.","Buckel S.D.","Groarke J.M.","Hope J.N.","Kingsley D.H.","Hermodson M.A."],"citationCrossReferences":[{"database":"PubMed","id":"3011793"},{"database":"DOI","id":"10.1016/s0021-9258(19)57448-8"}],"title":"The nucleotide sequences of the rbsD, rbsA, and rbsC genes of Escherichia coli K12.","publicationDate":"1986","journal":"J. Biol. Chem.","firstPage":"7652","lastPage":"7658","volume":"261"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"K12","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"7686882","citationType":"journal article","authors":["Burland V.D.","Plunkett G. III","Daniels D.L.","Blattner F.R."],"citationCrossReferences":[{"database":"PubMed","id":"7686882"},{"database":"DOI","id":"10.1006/geno.1993.1230"}],"title":"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication.","publicationDate":"1993","journal":"Genomics","firstPage":"551","lastPage":"561","volume":"16"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"6327617","citationType":"journal article","authors":["Iida A.","Harayama S.","Iino T.","Hazelbauer G.L."],"citationCrossReferences":[{"database":"PubMed","id":"6327617"},{"database":"DOI","id":"10.1128/jb.158.2.674-682.1984"}],"title":"Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12.","publicationDate":"1984","journal":"J. Bacteriol.","firstPage":"674","lastPage":"682","volume":"158"},"referencePositions":["FUNCTION"]},{"referenceNumber":6,"citation":{"id":"9922273","citationType":"journal article","authors":["Park Y.","Park C."],"citationCrossReferences":[{"database":"PubMed","id":"9922273"},{"database":"DOI","id":"10.1128/jb.181.3.1039-1042.1999"}],"title":"Topology of RbsC, a membrane component of the ribose transporter, belonging to the AraH superfamily.","publicationDate":"1999","journal":"J. Bacteriol.","firstPage":"1039","lastPage":"1042","volume":"181"},"referencePositions":["TOPOLOGY","SUBCELLULAR LOCATION"]},{"referenceNumber":7,"citation":{"id":"15919996","citationType":"journal article","authors":["Daley D.O.","Rapp M.","Granseth E.","Melen K.","Drew D.","von Heijne G."],"citationCrossReferences":[{"database":"PubMed","id":"15919996"},{"database":"DOI","id":"10.1126/science.1109730"}],"title":"Global topology analysis of the Escherichia coli inner membrane proteome.","publicationDate":"2005","journal":"Science","firstPage":"1321","lastPage":"1323","volume":"308"},"referencePositions":["TOPOLOGY [LARGE SCALE ANALYSIS]","SUBCELLULAR LOCATION"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":8,"citation":{"id":"25533465","citationType":"journal article","authors":["Clifton M.C.","Simon M.J.","Erramilli S.K.","Zhang H.","Zaitseva J.","Hermodson M.A.","Stauffacher C.V."],"citationCrossReferences":[{"database":"PubMed","id":"25533465"},{"database":"DOI","id":"10.1074/jbc.m114.621573"}],"title":"In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes.","publicationDate":"2015","journal":"J. Biol. Chem.","firstPage":"5555","lastPage":"5565","volume":"290"},"referencePositions":["FUNCTION","SUBUNIT"]},{"referenceNumber":9,"citation":{"id":"26305955","citationType":"journal article","authors":["Willett J.L.","Gucinski G.C.","Fatherree J.P.","Low D.A.","Hayes C.S."],"citationCrossReferences":[{"database":"PubMed","id":"26305955"},{"database":"DOI","id":"10.1073/pnas.1512124112"}],"title":"Contact-dependent growth inhibition toxins exploit multiple independent cell-entry pathways.","publicationDate":"2015","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"11341","lastPage":"11346","volume":"112"},"referencePositions":["RECEPTOR FOR CDI TOXIN ENTRY INTO TARGET CELL CYTOPLASM (MICROBIAL INFECTION)","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"K12 / MC4100 / ATCC 35695 / DSM 6574","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"M13169","properties":[{"key":"ProteinId","value":"AAA51474.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"L10328","properties":[{"key":"ProteinId","value":"AAA62103.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC76773.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE77538.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"G65178","properties":[{"key":"EntryName","value":"G65178"}]},{"database":"RefSeq","id":"NP_418206.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000211858.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP010438.1"}]},{"database":"AlphaFoldDB","id":"P0AGI1","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4263450","properties":[{"key":"Interactions","value":"34"}]},{"database":"ComplexPortal","id":"CPX-4284","properties":[{"key":"EntryName","value":"RbsABC ribose ABC transporter"}]},{"database":"FunCoup","id":"P0AGI1","properties":[{"key":"Number of interactors","value":"312"}]},{"database":"IntAct","id":"P0AGI1","properties":[{"key":"Interactions","value":"2"}]},{"database":"STRING","id":"511145.b3750","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"3.A.1.2.1","properties":[{"key":"FamilyName","value":"the atp-binding cassette (abc) superfamily"}]},{"database":"jPOST","id":"P0AGI1","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b3750","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC76773","properties":[{"key":"ProteinId","value":"AAC76773"},{"key":"GeneId","value":"b3750"}]},{"database":"GeneID","id":"93778199","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"948262","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW3729","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b3750","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_20315","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.2950","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB0809","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG1172","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_028880_2_2_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P0AGI1","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"SFYQMVV","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"8843934at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P0AGI1","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:RBSC-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:RBSC-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P0AGI1","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P0AGI1","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0043190","properties":[{"key":"GoTerm","value":"C:ATP-binding cassette (ABC) transporter complex"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"25533465"}]},{"database":"GO","id":"GO:0055052","properties":[{"key":"GoTerm","value":"C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"},{"key":"GoEvidenceType","value":"IPI:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"25533465"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"25533465"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15919996"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"9922273"}]},{"database":"GO","id":"GO:0015591","properties":[{"key":"GoTerm","value":"F:D-ribose transmembrane transporter activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"6327617"}]},{"database":"GO","id":"GO:0015752","properties":[{"key":"GoTerm","value":"P:D-ribose transmembrane transport"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"25533465"}]},{"database":"CDD","id":"cd06579","properties":[{"key":"EntryName","value":"TM_PBP1_transp_AraH_like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001851","properties":[{"key":"EntryName","value":"ABC_transp_permease"}]},{"database":"NCBIfam","id":"NF007067","properties":[{"key":"EntryName","value":"PRK09512.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR32196:SF21","properties":[{"key":"EntryName","value":"ABC TRANSPORTER PERMEASE PROTEIN YPHD-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR32196","properties":[{"key":"EntryName","value":"ABC TRANSPORTER PERMEASE PROTEIN YPHD-RELATED-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02653","properties":[{"key":"EntryName","value":"BPD_transp_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTINNLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVAAALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADLFGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNKIKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIVGTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ","length":321,"molWeight":33452,"crc64":"C7292EE5F0C78781","md5":"9F0BE77D9917763C8C42A8459A9228F5"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"SUBUNIT":1,"INTERACTION":2,"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":7,"Transmembrane":6,"Sequence conflict":1},"uniParcId":"UPI000003EB7D"}}