{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0AGG4","secondaryAccessions":["P33636","P76593","P77000","P77001"],"uniProtkbId":"THIO2_ECOLI","entryAudit":{"firstPublicDate":"2005-12-20","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2005-12-20","entryVersion":139,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Thioredoxin 2"},"shortNames":[{"value":"Trx-2"}],"ecNumbers":[{"value":"1.8.1.8"}]},"alternativeNames":[{"fullName":{"value":"Protein-disulfide reductase"}}]},"genes":[{"geneName":{"value":"trxC"},"synonyms":[{"value":"yfiG"}],"orderedLocusNames":[{"value":"b2582"},{"value":"JW2566"}]}],"comments":[{"texts":[{"value":"Efficient electron donor for the essential enzyme ribonucleotide reductase. Is also able to reduce the interchain disulfide bridges of insulin"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"[protein]-dithiol + NAD(+) = [protein]-disulfide + NADH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:18749"},{"database":"Rhea","id":"RHEA-COMP:10593"},{"database":"Rhea","id":"RHEA-COMP:10594"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29950"},{"database":"ChEBI","id":"CHEBI:50058"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"1.8.1.8"}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"[protein]-dithiol + NADP(+) = [protein]-disulfide + NADPH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:18753"},{"database":"Rhea","id":"RHEA-COMP:10593"},{"database":"Rhea","id":"RHEA-COMP:10594"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29950"},{"database":"ChEBI","id":"CHEBI:50058"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"}],"ecNumber":"1.8.1.8"}},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P0AGG4","intActId":"EBI-549392"},"interactantTwo":{"uniProtKBAccession":"P14900","geneName":"murD","intActId":"EBI-554780"},"numberOfExperiments":2,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the thioredoxin family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":139,"modifier":"EXACT"}},"description":"Thioredoxin 2","featureId":"PRO_0000120102"},{"type":"Domain","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":139,"modifier":"EXACT"}},"description":"Thioredoxin","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00691"}]},{"type":"Zinc finger","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":18,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Disulfide bond","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"Redox-active","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00691"}]}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0520","category":"Ligand","name":"NAD"},{"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"id":"KW-0676","category":"Domain","name":"Redox-active center"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0813","category":"Biological process","name":"Transport"},{"id":"KW-0862","category":"Ligand","name":"Zinc"},{"id":"KW-0863","category":"Domain","name":"Zinc-finger"}],"references":[{"referenceNumber":1,"citation":{"id":"9388228","citationType":"journal article","authors":["Miranda-Vizuete A.","Damdimopoulos A.E.","Gustafsson J.-A.","Spyrou G."],"citationCrossReferences":[{"database":"PubMed","id":"9388228"},{"database":"DOI","id":"10.1074/jbc.272.49.30841"}],"title":"Cloning, expression, and characterization of a novel Escherichia coli thioredoxin.","publicationDate":"1997","journal":"J. Biol. Chem.","firstPage":"30841","lastPage":"30847","volume":"272"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","CHARACTERIZATION"],"referenceComments":[{"value":"K12 / TG1","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9205837","citationType":"journal article","authors":["Yamamoto Y.","Aiba H.","Baba T.","Hayashi K.","Inada T.","Isono K.","Itoh T.","Kimura S.","Kitagawa M.","Makino K.","Miki T.","Mitsuhashi N.","Mizobuchi K.","Mori H.","Nakade S.","Nakamura Y.","Nashimoto H.","Oshima T.","Oyama S.","Saito N.","Sampei G.","Satoh Y.","Sivasundaram S.","Tagami H.","Takahashi H.","Takeda J.","Takemoto K.","Uehara K.","Wada C.","Yamagata S.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9205837"},{"database":"DOI","id":"10.1093/dnares/4.2.91"}],"title":"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.","publicationDate":"1997","journal":"DNA Res.","firstPage":"91","lastPage":"113","volume":"4"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]","SEQUENCE REVISION"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-F2PRDLKQ1MS6K","citationType":"book","authors":["Nashimoto H."],"title":"Non-ribosomal proteins affecting the assembly of ribosomes in Escherichia coli.","publicationDate":"1993","bookName":"The translational apparatus","editors":["Nierhaus K.H."],"firstPage":"185","lastPage":"195","publisher":"Plenum Press","address":"New York"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 37-104"],"referenceComments":[{"value":"K12","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"CI-5HM9JMGGS8TQI","citationType":"unpublished observations","authors":["Rudd K.E."],"publicationDate":"NOV-1993"},"referencePositions":["IDENTIFICATION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U85942","properties":[{"key":"ProteinId","value":"AAB88587.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC75635.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA16469.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"D13169","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"E65036","properties":[{"key":"EntryName","value":"E65036"}]},{"database":"RefSeq","id":"NP_417077.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001098726.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P0AGG4","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0AGG4","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4263420","properties":[{"key":"Interactions","value":"38"}]},{"database":"BioGRID","id":"851399","properties":[{"key":"Interactions","value":"2"}]},{"database":"DIP","id":"DIP-48115N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P0AGG4","properties":[{"key":"Number of interactors","value":"609"}]},{"database":"IntAct","id":"P0AGG4","properties":[{"key":"Interactions","value":"26"}]},{"database":"STRING","id":"511145.b2582","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P0AGG4","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b2582","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC75635","properties":[{"key":"ProteinId","value":"AAC75635"},{"key":"GeneId","value":"b2582"}]},{"database":"GeneID","id":"67175900","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"93774504","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"947062","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW2566","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b2582","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_14310","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.4152","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB1833","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG3118","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_090389_10_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P0AGG4","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"QRVDMIN","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9790390at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P0AGG4","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:RED-THIOREDOXIN2-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:RED-THIOREDOXIN2-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P0AGG4","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P0AGG4","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"9755155"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15911532"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18304323"}]},{"database":"GO","id":"GO:0047134","properties":[{"key":"GoTerm","value":"F:protein-disulfide reductase [NAD(P)H] activity"},{"key":"GoEvidenceType","value":"IMP:CACAO"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"10644706"}]},{"database":"GO","id":"GO:0015035","properties":[{"key":"GoTerm","value":"F:protein-disulfide reductase activity"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"9755155"}]},{"database":"GO","id":"GO:0008270","properties":[{"key":"GoTerm","value":"F:zinc ion binding"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12952960"}]},{"database":"CDD","id":"cd02947","properties":[{"key":"EntryName","value":"TRX_family"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.30.30.380:FF:000002","properties":[{"key":"EntryName","value":"Thioredoxin"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.30.10:FF:000001","properties":[{"key":"EntryName","value":"Thioredoxin"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.30.10","properties":[{"key":"EntryName","value":"Glutaredoxin"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.30.30.380","properties":[{"key":"EntryName","value":"Zn-finger domain of Sec23/24"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR049299","properties":[{"key":"EntryName","value":"Thio2_N"}]},{"database":"InterPro","id":"IPR005746","properties":[{"key":"EntryName","value":"Thioredoxin"}]},{"database":"InterPro","id":"IPR036249","properties":[{"key":"EntryName","value":"Thioredoxin-like_sf"}]},{"database":"InterPro","id":"IPR017937","properties":[{"key":"EntryName","value":"Thioredoxin_CS"}]},{"database":"InterPro","id":"IPR013766","properties":[{"key":"EntryName","value":"Thioredoxin_domain"}]},{"database":"NCBIfam","id":"NF008229","properties":[{"key":"EntryName","value":"PRK10996.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01068","properties":[{"key":"EntryName","value":"thioredoxin"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45663","properties":[{"key":"EntryName","value":"GEO12009P1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45663:SF40","properties":[{"key":"EntryName","value":"THIOREDOXIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00085","properties":[{"key":"EntryName","value":"Thioredoxin"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21352","properties":[{"key":"EntryName","value":"Zn_ribbon_Thio2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00421","properties":[{"key":"EntryName","value":"THIOREDOXIN"}]},{"database":"SUPFAM","id":"SSF52833","properties":[{"key":"EntryName","value":"Thioredoxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00194","properties":[{"key":"EntryName","value":"THIOREDOXIN_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51352","properties":[{"key":"EntryName","value":"THIOREDOXIN_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGETLDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRFGIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL","length":139,"molWeight":15555,"crc64":"4C973F6FE55C8856","md5":"E9D50CFBFAEA17E8EBB59F9ADD77F07A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Zinc finger":1,"Disulfide bond":1},"uniParcId":"UPI0000136D76"}}