{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0AG24","secondaryAccessions":["P17580","Q2M7W3"],"uniProtkbId":"SPOT_ECOLI","entryAudit":{"firstPublicDate":"2005-12-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-12-20","entryVersion":154,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Bifunctional (p)ppGpp synthase/hydrolase SpoT"}},"includes":[{"recommendedName":{"fullName":{"value":"GTP pyrophosphokinase"},"ecNumbers":[{"value":"2.7.6.5"}]},"alternativeNames":[{"fullName":{"value":"(p)ppGpp synthase"}},{"fullName":{"value":"ATP:GTP 3'-pyrophosphotransferase"}},{"fullName":{"value":"Stringent response-like protein"}},{"fullName":{"value":"ppGpp synthase II"}}]},{"recommendedName":{"fullName":{"value":"Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase"},"ecNumbers":[{"value":"3.1.7.2"}]},"alternativeNames":[{"fullName":{"value":"Penta-phosphate guanosine-3'-pyrophosphohydrolase"},"shortNames":[{"value":"(ppGpp)ase"}]}]}]},"genes":[{"geneName":{"value":"spoT"},"orderedLocusNames":[{"value":"b3650"},{"value":"JW3625"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14622409"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19460094"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21488981"}],"value":"In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response which coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the synthesis and degradation of ppGpp. The second messengers ppGpp and c-di-GMP together control biofilm formation in response to translational stress; ppGpp represses biofilm formation while c-di-GMP induces it. ppGpp activates transcription of CsrA-antagonistic small RNAs CsrB and CsrC, which down-regulate CsrA's action on translation during the stringent response (PubMed:21488981)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"GTP + ATP = guanosine 3'-diphosphate 5'-triphosphate + AMP","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:22088"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:37565"},{"database":"ChEBI","id":"CHEBI:142410"},{"database":"ChEBI","id":"CHEBI:456215"}],"ecNumber":"2.7.6.5"}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"guanosine 3',5'-bis(diphosphate) + H2O = GDP + diphosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:14253"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58189"},{"database":"ChEBI","id":"CHEBI:77828"}],"ecNumber":"3.1.7.2"}},{"commentType":"COFACTOR","cofactors":[{"name":"Mn(2+)","cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}]},{"texts":[{"value":"Purine metabolism; ppGpp biosynthesis; ppGpp from GDP: step 1/1"}],"commentType":"PATHWAY"},{"texts":[{"value":"Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15292126"}],"value":"Interacts with ObgE/CgtA (AC P42641); this association is not required for pre-50S ribosome binding. Under both amino acid and carbon starvation conditions about half of the protein dissociates from the ribosome"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P0AG24","intActId":"EBI-543228"},"interactantTwo":{"uniProtKBAccession":"P0A6A8","geneName":"acpP","intActId":"EBI-542566"},"numberOfExperiments":6,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"value":"Is associated with pre-50S ribosomal subunits in a salt-dependent manner"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17616600"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14622409"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19460094"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21488981"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26051177"}],"value":"In the presence of wild-type relA, spoT cannot be deleted because ppGpp accumulation is toxic. In the double relA/spoT deletion (a ppGpp0 mutant) there is a very large increase in biofilm formation (in a csrA-disrupted background) (PubMed:19460094). The ppGpp0 mutant makes decreased levels of CsrA and its inhibitory small RNAs (sRNA) CsrB and CsrC (PubMed:21488981). The double relA/spoT deletion obviates persister cell formation in a hipA7 mutant (PubMed:14622409, PubMed:26051177)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the RelA/SpoT family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":702,"modifier":"EXACT"}},"description":"Bifunctional (p)ppGpp synthase/hydrolase SpoT","featureId":"PRO_0000166569"},{"type":"Domain","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"HD","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01175"}]},{"type":"Domain","location":{"start":{"value":386,"modifier":"EXACT"},"end":{"value":447,"modifier":"EXACT"}},"description":"TGS","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01228"}]},{"type":"Domain","location":{"start":{"value":628,"modifier":"EXACT"},"end":{"value":702,"modifier":"EXACT"}},"description":"ACT","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01007"}]},{"type":"Mutagenesis","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"Obviates hydrolysis of ppGpp, moderately decreases biofilm formation, loss of biofilm induction in response to translation inhibitors.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19460094"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["N"]}},{"type":"Mutagenesis","location":{"start":{"value":259,"modifier":"EXACT"},"end":{"value":259,"modifier":"EXACT"}},"description":"Obviates synthesis of ppGpp, strongly increases biofilm formation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19460094"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["N"]}},{"type":"Mutagenesis","location":{"start":{"value":293,"modifier":"EXACT"},"end":{"value":293,"modifier":"EXACT"}},"description":"Unable to restore (p)ppGpp synthesis nor complement a relA/spoT double disruption.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12596879"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":294,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"Synthesizes (p)ppGpp, complements a relA/spoT double disruption.","alternativeSequence":{"originalSequence":"Y","alternativeSequences":["I"]}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"2549050","citationType":"journal article","authors":["Sarubbi E.","Rudd K.E.","Xiao H.","Ikehara K.","Kalman M.","Cashel M."],"citationCrossReferences":[{"database":"PubMed","id":"2549050"},{"database":"DOI","id":"10.1016/s0021-9258(18)63813-x"}],"title":"Characterization of the spoT gene of Escherichia coli.","publicationDate":"1989","journal":"J. Biol. Chem.","firstPage":"15074","lastPage":"15082","volume":"264"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":2,"citation":{"id":"7686882","citationType":"journal article","authors":["Burland V.D.","Plunkett G. III","Daniels D.L.","Blattner F.R."],"citationCrossReferences":[{"database":"PubMed","id":"7686882"},{"database":"DOI","id":"10.1006/geno.1993.1230"}],"title":"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication.","publicationDate":"1993","journal":"Genomics","firstPage":"551","lastPage":"561","volume":"16"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"2005134","citationType":"journal article","authors":["Xiao H.","Kalman M.","Ikehara K.","Zemel S.","Glaser G.","Cashel M."],"citationCrossReferences":[{"database":"PubMed","id":"2005134"},{"database":"DOI","id":"10.1016/s0021-9258(19)67694-5"}],"title":"Residual guanosine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations.","publicationDate":"1991","journal":"J. Biol. Chem.","firstPage":"5980","lastPage":"5990","volume":"266"},"referencePositions":["PROBABLE (P)PPGPP SYNTHESIS ACTIVITY"]},{"referenceNumber":6,"citation":{"id":"12596879","citationType":"journal article","authors":["Fujita C.","Maeda M.","Fujii T.","Iwamoto R.","Ikehara K."],"citationCrossReferences":[{"database":"PubMed","id":"12596879"},{"database":"DOI","id":"10.1271/bbb.66.2735"}],"title":"Identification of an indispensable amino acid for ppGpp synthesis of Escherichia coli SpoT protein.","publicationDate":"2002","journal":"Biosci. Biotechnol. Biochem.","firstPage":"2735","lastPage":"2738","volume":"66"},"referencePositions":["PPGPP SYNTHASE ACTIVITY","MUTAGENESIS OF ASP-293"],"referenceComments":[{"value":"CF8295","type":"STRAIN"}]},{"referenceNumber":7,"citation":{"id":"14622409","citationType":"journal article","authors":["Korch S.B.","Henderson T.A.","Hill T.M."],"citationCrossReferences":[{"database":"PubMed","id":"14622409"},{"database":"DOI","id":"10.1046/j.1365-2958.2003.03779.x"}],"title":"Characterization of the hipA7 allele of Escherichia coli and evidence that high persistence is governed by (p)ppGpp synthesis.","publicationDate":"2003","journal":"Mol. Microbiol.","firstPage":"1199","lastPage":"1213","volume":"50"},"referencePositions":["FUNCTION IN PERSISTENCE","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":8,"citation":{"id":"15292126","citationType":"journal article","authors":["Wout P.","Pu K.","Sullivan S.M.","Reese V.","Zhou S.","Lin B.","Maddock J.R."],"citationCrossReferences":[{"database":"PubMed","id":"15292126"},{"database":"DOI","id":"10.1128/jb.186.16.5249-5257.2004"}],"title":"The Escherichia coli GTPase CgtAE cofractionates with the 50S ribosomal subunit and interacts with SpoT, a ppGpp synthetase/hydrolase.","publicationDate":"2004","journal":"J. Bacteriol.","firstPage":"5249","lastPage":"5257","volume":"186"},"referencePositions":["INTERACTION WITH OBGE/CGTA"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":9,"citation":{"id":"17616600","citationType":"journal article","authors":["Jiang M.","Sullivan S.M.","Wout P.K.","Maddock J.R."],"citationCrossReferences":[{"database":"PubMed","id":"17616600"},{"database":"DOI","id":"10.1128/jb.00315-07"}],"title":"G-protein control of the ribosome-associated stress response protein SpoT.","publicationDate":"2007","journal":"J. Bacteriol.","firstPage":"6140","lastPage":"6147","volume":"189"},"referencePositions":["SUBCELLULAR LOCATION","LARGE RIBOSOMAL SUBUNIT ASSOCIATION","CHARACTERIZATION UNDER AMINO ACID OR CARBON STARVATION"],"referenceComments":[{"value":"K12","type":"STRAIN"}]},{"referenceNumber":10,"citation":{"id":"19460094","citationType":"journal article","authors":["Boehm A.","Steiner S.","Zaehringer F.","Casanova A.","Hamburger F.","Ritz D.","Keck W.","Ackermann M.","Schirmer T.","Jenal U."],"citationCrossReferences":[{"database":"PubMed","id":"19460094"},{"database":"DOI","id":"10.1111/j.1365-2958.2009.06739.x"}],"title":"Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress.","publicationDate":"2009","journal":"Mol. Microbiol.","firstPage":"1500","lastPage":"1516","volume":"72"},"referencePositions":["FUNCTION IN BIOFILM FORMATION","MUTAGENESIS OF ASP-73 AND ASP-259","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"K12 / MG1655 / AB400","type":"STRAIN"}]},{"referenceNumber":11,"citation":{"id":"21488981","citationType":"journal article","authors":["Edwards A.N.","Patterson-Fortin L.M.","Vakulskas C.A.","Mercante J.W.","Potrykus K.","Vinella D.","Camacho M.I.","Fields J.A.","Thompson S.A.","Georgellis D.","Cashel M.","Babitzke P.","Romeo T."],"citationCrossReferences":[{"database":"PubMed","id":"21488981"},{"database":"DOI","id":"10.1111/j.1365-2958.2011.07663.x"}],"title":"Circuitry linking the Csr and stringent response global regulatory systems.","publicationDate":"2011","journal":"Mol. Microbiol.","firstPage":"1561","lastPage":"1580","volume":"80"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"K12 / CF7789","type":"STRAIN"},{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":12,"citation":{"id":"26051177","citationType":"journal article","authors":["Verstraeten N.","Knapen W.J.","Kint C.I.","Liebens V.","Van den Bergh B.","Dewachter L.","Michiels J.E.","Fu Q.","David C.C.","Fierro A.C.","Marchal K.","Beirlant J.","Versees W.","Hofkens J.","Jansen M.","Fauvart M.","Michiels J."],"citationCrossReferences":[{"database":"PubMed","id":"26051177"},{"database":"DOI","id":"10.1016/j.molcel.2015.05.011"}],"title":"Obg and membrane depolarization are part of a microbial bet-hedging strategy that leads to antibiotic tolerance.","publicationDate":"2015","journal":"Mol. Cell","firstPage":"9","lastPage":"21","volume":"59"},"referencePositions":["FUNCTION IN PERSISTENCE","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"K12 / BW25113","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"M24503","properties":[{"key":"ProteinId","value":"AAB00160.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"L10328","properties":[{"key":"ProteinId","value":"AAA62003.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC76674.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE77643.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"B30374","properties":[{"key":"EntryName","value":"SHECGD"}]},{"database":"RefSeq","id":"NP_418107.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000280488.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P0AG24","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0AG24","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4259361","properties":[{"key":"Interactions","value":"497"}]},{"database":"DIP","id":"DIP-29378N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P0AG24","properties":[{"key":"Number of interactors","value":"777"}]},{"database":"IntAct","id":"P0AG24","properties":[{"key":"Interactions","value":"61"}]},{"database":"STRING","id":"511145.b3650","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P0AG24","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b3650","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC76674","properties":[{"key":"ProteinId","value":"AAC76674"},{"key":"GeneId","value":"b3650"}]},{"database":"GeneID","id":"93778365","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"948159","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW3625","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b3650","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.3056","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB0959","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0317","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_012300_3_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P0AG24","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"PIDFAYS","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PhylomeDB","id":"P0AG24","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:SPOT-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:SPOT-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"2.7.6.5","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"BRENDA","id":"3.1.7.2","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"UniPathway","id":"UPA00908","properties":[{"key":"RectionId","value":"UER00884"}]},{"database":"UniPathway","id":"UPA00908","properties":[{"key":"RectionId","value":"UER00886"}]},{"database":"PRO","id":"PR:P0AG24","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P0AG24","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18304323"}]},{"database":"GO","id":"GO:0008728","properties":[{"key":"GoTerm","value":"F:GTP diphosphokinase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0008893","properties":[{"key":"GoTerm","value":"F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"365225"}]},{"database":"GO","id":"GO:0015970","properties":[{"key":"GoTerm","value":"P:guanosine tetraphosphate biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"GO","id":"GO:0015969","properties":[{"key":"GoTerm","value":"P:guanosine tetraphosphate metabolic process"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0015949","properties":[{"key":"GoTerm","value":"P:nucleobase-containing small molecule interconversion"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"319345"}]},{"database":"GO","id":"GO:0042594","properties":[{"key":"GoTerm","value":"P:response to starvation"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd04876","properties":[{"key":"EntryName","value":"ACT_RelA-SpoT"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00077","properties":[{"key":"EntryName","value":"HDc"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd05399","properties":[{"key":"EntryName","value":"NT_Rel-Spo_like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01668","properties":[{"key":"EntryName","value":"TGS_RSH"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.70.260:FF:000006","properties":[{"key":"EntryName","value":"(P)ppGpp synthase/hydrolase SpoT"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.10.20.30:FF:000002","properties":[{"key":"EntryName","value":"GTP pyrophosphokinase (RelA/SpoT)"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.3210.10:FF:000001","properties":[{"key":"EntryName","value":"GTP pyrophosphokinase RelA"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.460.10:FF:000001","properties":[{"key":"EntryName","value":"GTP pyrophosphokinase RelA"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.10.20.30","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.260","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.460.10","properties":[{"key":"EntryName","value":"Beta Polymerase, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.3210.10","properties":[{"key":"EntryName","value":"Hypothetical protein af1432"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR045865","properties":[{"key":"EntryName","value":"ACT-like_dom_sf"}]},{"database":"InterPro","id":"IPR002912","properties":[{"key":"EntryName","value":"ACT_dom"}]},{"database":"InterPro","id":"IPR012675","properties":[{"key":"EntryName","value":"Beta-grasp_dom_sf"}]},{"database":"InterPro","id":"IPR003607","properties":[{"key":"EntryName","value":"HD/PDEase_dom"}]},{"database":"InterPro","id":"IPR006674","properties":[{"key":"EntryName","value":"HD_domain"}]},{"database":"InterPro","id":"IPR043519","properties":[{"key":"EntryName","value":"NT_sf"}]},{"database":"InterPro","id":"IPR004811","properties":[{"key":"EntryName","value":"RelA/Spo_fam"}]},{"database":"InterPro","id":"IPR045600","properties":[{"key":"EntryName","value":"RelA/SpoT_AH_RIS"}]},{"database":"InterPro","id":"IPR060374","properties":[{"key":"EntryName","value":"RelA_RIS"}]},{"database":"InterPro","id":"IPR007685","properties":[{"key":"EntryName","value":"RelA_SpoT"}]},{"database":"InterPro","id":"IPR004095","properties":[{"key":"EntryName","value":"TGS"}]},{"database":"InterPro","id":"IPR012676","properties":[{"key":"EntryName","value":"TGS-like"}]},{"database":"InterPro","id":"IPR033655","properties":[{"key":"EntryName","value":"TGS_RelA/SpoT"}]},{"database":"NCBIfam","id":"NF008303","properties":[{"key":"EntryName","value":"PRK11092.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00691","properties":[{"key":"EntryName","value":"spoT_relA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21262:SF36","properties":[{"key":"EntryName","value":"BIFUNCTIONAL (P)PPGPP SYNTHASE_HYDROLASE SPOT"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21262","properties":[{"key":"EntryName","value":"GUANOSINE-3',5'-BIS DIPHOSPHATE 3'-PYROPHOSPHOHYDROLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13291","properties":[{"key":"EntryName","value":"ACT_4"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13328","properties":[{"key":"EntryName","value":"HD_4"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF19296","properties":[{"key":"EntryName","value":"RelA_AH"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF28438","properties":[{"key":"EntryName","value":"RelA_RIS"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04607","properties":[{"key":"EntryName","value":"RelA_SpoT"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02824","properties":[{"key":"EntryName","value":"TGS"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00471","properties":[{"key":"EntryName","value":"HDc"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00954","properties":[{"key":"EntryName","value":"RelA_SpoT"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55021","properties":[{"key":"EntryName","value":"ACT-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF109604","properties":[{"key":"EntryName","value":"HD-domain/PDEase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81301","properties":[{"key":"EntryName","value":"Nucleotidyltransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81271","properties":[{"key":"EntryName","value":"TGS-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51671","properties":[{"key":"EntryName","value":"ACT"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51831","properties":[{"key":"EntryName","value":"HD"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51880","properties":[{"key":"EntryName","value":"TGS"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENIQRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQEFITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN","length":702,"molWeight":79342,"crc64":"A85F70F57D082EE8","md5":"F94417311CCD06512CC8ECFA521BBBE6"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":1,"PATHWAY":2,"SUBUNIT":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":3,"Mutagenesis":4},"uniParcId":"UPI000003EB28"}}