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May influence peptidoglycan synthesis and/or repair through PBP1B-dependent processes (PubMed:41721466). May function in the periplasmic phase of peptidoglycan synthesis, likely at the step of peptidoglycan polymerization (PubMed:40402998). SanA works independently of ElyC and the synthesis of the enterobacterial common antigen (ECA) (PubMed:40402998)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"41721466"}],"value":"Interacts with the penicillin-binding protein 1B (PBP1B), a non-essential yet physiologically important peptidoglycan synthase"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"40402998"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"8550448"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Single-pass membrane protein","id":"SL-9904"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40402998"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"41721466"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8550448"}],"value":"Deletion of the gene leads to a high level of nascent peptidoglycan strand incorporation into the sacculi, and restores growth and viability to several mutants defective in either cell division or cell elongation (PubMed:40402998). The mutant is sensitive to vancomycin and treatment with SDS and EDTA at high temperature, and exhibits reduced growth rate in low-osmolarity media, indicating compromised cell envelope integrity (PubMed:40402998). Only vancomycin susceptibility is altered by SanA deficiency while susceptibility to other antibiotics is unaffected (PubMed:41721466). A strain carrying a null mutation of the gene, transferred to the E.coli chromosome by homologous recombination, is perfectly viable, but after two generations at high temperature (43 degrees Celsius), the barrier function of its envelope towards vancomycin is defective (PubMed:8550448)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"41721466"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8550448"}],"value":"Overexpression of the gene complements the permeability defects of a vancomycin-susceptible mutant in E.coli (PubMed:8550448). Overproduction confers resistance to vancomycin but does not affect susceptibility to mecillinam, and the cells appear only slightly more sensitive to aztreonam (PubMed:41721466). Overproduction changes the cell shape from rod to round, with some round cells being lysed at the cell poles (PubMed:41721466)"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the vancomycin-sensitivity protein (SanA) (TC 9.B.114) family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":239,"modifier":"EXACT"}},"description":"Peptidoglycan biosynthesis regulator SanA","featureId":"PRO_0000097576"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":4,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"40402998"}]},{"type":"Transmembrane","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":239,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"40402998"}]},{"type":"Mutagenesis","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"Loss of function. 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