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In vitro, can also deaminate 2-aminoacrylate (PubMed:33839153)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(Z)-3-aminoacrylate + H2O + H(+) = 3-oxopropanoate + NH4(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:34947"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:28938"},{"database":"ChEBI","id":"CHEBI:33190"},{"database":"ChEBI","id":"CHEBI:59894"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33839153"}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":9.1,"unit":"mM","substrate":"3-aminoacrylate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33839153"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33839153"}],"value":"kcat is 52 min(-1) with 3-aminoacrylate as substrate."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00831"}],"value":"Homotrimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11121068"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17919280"}],"value":"Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20400551"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33839153"}],"value":"Cells lacking this gene fail to grow on uridine as the sole source of nitrogen at room temperature (PubMed:20400551, PubMed:33839153). Expression of rutC in trans did not restore growth (PubMed:33839153). However, a plasmid carrying the complete rutCDEFG operon restored growth of the deletion mutant with uridine as sole nitrogen source, suggesting that the lesion in rutC was polar on downstream gene(s) required for rut pathway function (PubMed:33839153)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33839153"}],"value":"This reaction can occur spontaneously. Under standard laboratory conditions, a Rut pathway lacking RutC generates sufficient nitrogen from uracil for growth of E.coli"}],"commentType":"MISCELLANEOUS"},{"texts":[{"value":"The Rut pathway degrades exogenous pyrimidines as the sole nitrogen source at room temperature but not at 37 degrees Celsius, a restriction that is apparently a consequence of an inadequate ability to remove toxic malonic semialdehyde at the higher temperature (RutE/YdfG function)"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00831"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the RutC family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":128,"modifier":"EXACT"}},"description":"3-aminoacrylate deaminase RutC","featureId":"PRO_0000170319"},{"type":"Mutagenesis","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"Loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33839153"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"8905232","citationType":"journal article","authors":["Oshima T.","Aiba H.","Baba T.","Fujita K.","Hayashi K.","Honjo A.","Ikemoto K.","Inada T.","Itoh T.","Kajihara M.","Kanai K.","Kashimoto K.","Kimura S.","Kitagawa M.","Makino K.","Masuda S.","Miki T.","Mizobuchi K.","Mori H.","Motomura K.","Nakamura Y.","Nashimoto H.","Nishio Y.","Saito N.","Sampei G.","Seki Y.","Tagami H.","Takemoto K.","Wada C.","Yamamoto Y.","Yano M.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"8905232"},{"database":"DOI","id":"10.1093/dnares/3.3.137"}],"title":"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.","publicationDate":"1996","journal":"DNA Res.","firstPage":"137","lastPage":"155","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. 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