{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0AFE4","secondaryAccessions":["P33606","P76487","P78182"],"uniProtkbId":"NUOK_ECOLI","entryAudit":{"firstPublicDate":"2005-12-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-12-20","entryVersion":136,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01456"}],"value":"NADH-quinone oxidoreductase subunit K"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01456"}],"value":"7.1.1.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01456"}],"value":"NADH dehydrogenase I subunit K"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01456"}],"value":"NDH-1 subunit K"}},{"fullName":{"value":"NUO11"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01456"}],"value":"nuoK"},"orderedLocusNames":[{"value":"b2279"},{"value":"JW2274"}]}],"comments":[{"texts":[{"value":"NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient"}],"commentType":"FUNCTION"},{"texts":[{"value":"There are 2 NADH dehydrogenases in E.coli, however only this complex is able to use dNADH (reduced nicotinamide hypoxanthine dinucleotide, deamino-NADH) and dNADH-DB (dimethoxy-5-methyl-6-decyl-1,4-benzoquinone) as substrates"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a quinone + NADH + 5 H(+)(in) = a quinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:57888"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:24646"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:132124"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01456"}]}},{"texts":[{"value":"NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Cell inner membrane","id":"SL-0037"},"topology":{"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01456"}],"value":"Belongs to the complex I subunit 4L family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"NADH-quinone oxidoreductase subunit K","featureId":"PRO_0000118524"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":3,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01456"}]},{"type":"Topological domain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01456"}]},{"type":"Topological domain","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":80,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01456"}]},{"type":"Topological domain","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Mutagenesis","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"Retains 14% dNADH oxidase and dNADH-DB reductase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15996109"}],"alternativeSequence":{"originalSequence":"RR","alternativeSequences":["AA"]}},{"type":"Mutagenesis","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"Retains 2% dNADH oxidase and dNADH-DB reductase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15996109"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"Retains 2% dNADH oxidase and dNADH-DB reductase activity, neither a membrane potential nor a proton gradient can be formed by isolated membranes.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15996109"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"Retains 43% dNADH oxidase and dNADH-DB reductase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15996109"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"Retains 22% dNADH oxidase and dNADH-DB reductase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15996109"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["Q"]}},{"type":"Sequence conflict","location":{"start":{"value":40,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"in Ref. 1; CAA48370","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"NA","alternativeSequences":["RP"]}},{"type":"Sequence conflict","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"in Ref. 1; CAA48370","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"FVVAGSYWGQTDGQ","alternativeSequences":["LRGRRKLLGPRPTVK"]}},{"type":"Sequence conflict","location":{"start":{"value":67,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"in Ref. 1; CAA48370","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["T"]}},{"type":"Helix","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":92,"modifier":"EXACT"},"end":{"value":94,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0520","category":"Ligand","name":"NAD"},{"id":"KW-0874","category":"PTM","name":"Quinone"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"},{"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"7690854","citationType":"journal article","authors":["Weidner U.","Geier S.","Ptock A.","Friedrich T.","Leif H.","Weiss H."],"citationCrossReferences":[{"database":"PubMed","id":"7690854"},{"database":"DOI","id":"10.1006/jmbi.1993.1488"}],"title":"The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I.","publicationDate":"1993","journal":"J. Mol. Biol.","firstPage":"109","lastPage":"122","volume":"233"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"K12 / AN387","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9205837","citationType":"journal article","authors":["Yamamoto Y.","Aiba H.","Baba T.","Hayashi K.","Inada T.","Isono K.","Itoh T.","Kimura S.","Kitagawa M.","Makino K.","Miki T.","Mitsuhashi N.","Mizobuchi K.","Mori H.","Nakade S.","Nakamura Y.","Nashimoto H.","Oshima T.","Oyama S.","Saito N.","Sampei G.","Satoh Y.","Sivasundaram S.","Tagami H.","Takahashi H.","Takeda J.","Takemoto K.","Uehara K.","Wada C.","Yamagata S.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9205837"},{"database":"DOI","id":"10.1093/dnares/4.2.91"}],"title":"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.","publicationDate":"1997","journal":"DNA Res.","firstPage":"91","lastPage":"113","volume":"4"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"15996109","citationType":"journal article","authors":["Kao M.-C.","Nakamaru-Ogiso E.","Matsuno-Yagi A.","Yagi T."],"citationCrossReferences":[{"database":"PubMed","id":"15996109"},{"database":"DOI","id":"10.1021/bi050708w"}],"title":"Characterization of the membrane domain subunit NuoK (ND4L) of the NADH-quinone oxidoreductase from Escherichia coli.","publicationDate":"2005","journal":"Biochemistry","firstPage":"9545","lastPage":"9554","volume":"44"},"referencePositions":["MUTAGENESIS OF 25-ARG-ARG-26; GLU-36 AND GLU-72"],"referenceComments":[{"value":"K12 / MC4100 / ATCC 35695 / DSM 6574","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"15919996","citationType":"journal article","authors":["Daley D.O.","Rapp M.","Granseth E.","Melen K.","Drew D.","von Heijne G."],"citationCrossReferences":[{"database":"PubMed","id":"15919996"},{"database":"DOI","id":"10.1126/science.1109730"}],"title":"Global topology analysis of the Escherichia coli inner membrane proteome.","publicationDate":"2005","journal":"Science","firstPage":"1321","lastPage":"1323","volume":"308"},"referencePositions":["TOPOLOGY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X68301","properties":[{"key":"ProteinId","value":"CAA48370.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC75339.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA16107.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"E64999","properties":[{"key":"EntryName","value":"E64999"}]},{"database":"PIR","id":"S38320","properties":[{"key":"EntryName","value":"S37068"}]},{"database":"RefSeq","id":"NP_416782.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000612644.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"PDB","id":"7NYH","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.60 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7NYR","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.30 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7NYU","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.80 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7NYV","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.70 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7P61","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.20 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7P62","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.60 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7P63","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.40 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7P64","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.50 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7P69","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7P7C","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.40 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7P7E","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7P7J","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7P7K","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.10 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7P7L","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7P7M","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.20 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7Z7R","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.36 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7Z7S","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.40 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7Z7T","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.10 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7Z7V","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.29 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7Z80","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.93 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7Z83","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.88 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7Z84","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.87 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7ZC5","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"7ZCI","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.69 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"9Q8I","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.35 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"9TAJ","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"9TAK","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.80 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"9TAM","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.96 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDB","id":"9TAO","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.61 A"},{"key":"Chains","value":"K=1-100"}]},{"database":"PDBsum","id":"7NYH","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7NYR","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7NYU","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7NYV","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P61","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P62","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P63","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P64","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P69","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7C","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7E","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7J","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7K","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7L","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7M","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7R","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7S","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7T","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7V","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z80","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z83","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z84","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7ZC5","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7ZCI","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9Q8I","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAJ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAK","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAM","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAO","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P0AFE4","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-12653","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-12654","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-12655","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-52895","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0AFE4","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4260508","properties":[{"key":"Interactions","value":"70"}]},{"database":"ComplexPortal","id":"CPX-243","properties":[{"key":"EntryName","value":"Respiratory chain complex I"}]},{"database":"DIP","id":"DIP-51165N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P0AFE4","properties":[{"key":"Number of interactors","value":"349"}]},{"database":"IntAct","id":"P0AFE4","properties":[{"key":"Interactions","value":"2"}]},{"database":"STRING","id":"511145.b2279","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"3.D.1.1.1","properties":[{"key":"FamilyName","value":"the h+ or na+-translocating nadh dehydrogenase (ndh) family"}]},{"database":"jPOST","id":"P0AFE4","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b2279","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC75339","properties":[{"key":"ProteinId","value":"AAC75339"},{"key":"GeneId","value":"b2279"}]},{"database":"GeneID","id":"67176151","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"93033872","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"947580","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW2274","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b2279","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_12720","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.4457","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB2015","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0713","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_144724_0_1_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P0AFE4","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"IPMEHGL","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9801357at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P0AFE4","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:NUOK-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:NUOK-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P0AFE4","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P0AFE4","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16079137"}]},{"database":"GO","id":"GO:0030964","properties":[{"key":"GoTerm","value":"C:NADH dehydrogenase complex"},{"key":"GoEvidenceType","value":"IDA:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"7607227"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15919996"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17309111"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"7607227"}]},{"database":"GO","id":"GO:0045271","properties":[{"key":"GoTerm","value":"C:respiratory chain complex I"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17722886"}]},{"database":"GO","id":"GO:0050136","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (quinone) (non-electrogenic) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"15996109"}]},{"database":"GO","id":"GO:0042773","properties":[{"key":"GoTerm","value":"P:ATP synthesis coupled electron transport"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0022904","properties":[{"key":"GoTerm","value":"P:respiratory electron transport chain"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18316732"}]},{"database":"FunFam","id":"1.10.287.3510:FF:000001","properties":[{"key":"EntryName","value":"NADH-quinone oxidoreductase subunit K"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.287.3510","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01456","properties":[{"key":"EntryName","value":"NDH1_NuoK"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001133","properties":[{"key":"EntryName","value":"NADH_UbQ_OxRdtase_chain4L/K"}]},{"database":"InterPro","id":"IPR039428","properties":[{"key":"EntryName","value":"NUOK/Mnh_C1-like"}]},{"database":"NCBIfam","id":"NF004319","properties":[{"key":"EntryName","value":"PRK05715.1-1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF004320","properties":[{"key":"EntryName","value":"PRK05715.1-2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11434:SF16","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4L"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11434","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT ND4L"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00420","properties":[{"key":"EntryName","value":"Oxidored_q2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASALAFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG","length":100,"molWeight":10845,"crc64":"CC5AF3946A879383","md5":"26E88BB20AA45666BA298FD045B8A027"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":4,"Transmembrane":3,"Mutagenesis":5,"Sequence conflict":3,"Helix":4},"uniParcId":"UPI0000130A0D"}}