{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0AFE0","secondaryAccessions":["P33605","P78236"],"uniProtkbId":"NUOJ_ECOLI","entryAudit":{"firstPublicDate":"2005-12-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-12-20","entryVersion":130,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"NADH-quinone oxidoreductase subunit J"},"ecNumbers":[{"value":"7.1.1.-"}]},"alternativeNames":[{"fullName":{"value":"NADH dehydrogenase I subunit J"}},{"fullName":{"value":"NDH-1 subunit J"}},{"fullName":{"value":"NUO10"}}]},"genes":[{"geneName":{"value":"nuoJ"},"orderedLocusNames":[{"value":"b2280"},{"value":"JW2275"}]}],"comments":[{"texts":[{"value":"NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a quinone + NADH + 5 H(+)(in) = a quinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:57888"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:24646"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:132124"}]}},{"texts":[{"value":"Composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Cell inner membrane","id":"SL-0037"},"topology":{"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the complex I subunit 6 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"NADH-quinone oxidoreductase subunit J","featureId":"PRO_0000118370"},{"type":"Transmembrane","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":92,"modifier":"EXACT"},"end":{"value":112,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":138,"modifier":"EXACT"},"end":{"value":158,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":159,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Sequence conflict","location":{"start":{"value":85,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"in Ref. 1; CAA48369","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["T"]}},{"type":"Sequence conflict","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":126,"modifier":"EXACT"}},"description":"in Ref. 1; CAA48369","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["R"]}},{"type":"Helix","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":45,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":80,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":90,"modifier":"EXACT"},"end":{"value":111,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Turn","location":{"start":{"value":134,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":138,"modifier":"EXACT"},"end":{"value":158,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":172,"modifier":"EXACT"},"end":{"value":174,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7P61"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0520","category":"Ligand","name":"NAD"},{"id":"KW-0874","category":"PTM","name":"Quinone"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"7690854","citationType":"journal article","authors":["Weidner U.","Geier S.","Ptock A.","Friedrich T.","Leif H.","Weiss H."],"citationCrossReferences":[{"database":"PubMed","id":"7690854"},{"database":"DOI","id":"10.1006/jmbi.1993.1488"}],"title":"The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I.","publicationDate":"1993","journal":"J. Mol. Biol.","firstPage":"109","lastPage":"122","volume":"233"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"K12 / AN387","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9205837","citationType":"journal article","authors":["Yamamoto Y.","Aiba H.","Baba T.","Hayashi K.","Inada T.","Isono K.","Itoh T.","Kimura S.","Kitagawa M.","Makino K.","Miki T.","Mitsuhashi N.","Mizobuchi K.","Mori H.","Nakade S.","Nakamura Y.","Nashimoto H.","Oshima T.","Oyama S.","Saito N.","Sampei G.","Satoh Y.","Sivasundaram S.","Tagami H.","Takahashi H.","Takeda J.","Takemoto K.","Uehara K.","Wada C.","Yamagata S.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9205837"},{"database":"DOI","id":"10.1093/dnares/4.2.91"}],"title":"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.","publicationDate":"1997","journal":"DNA Res.","firstPage":"91","lastPage":"113","volume":"4"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"15919996","citationType":"journal article","authors":["Daley D.O.","Rapp M.","Granseth E.","Melen K.","Drew D.","von Heijne G."],"citationCrossReferences":[{"database":"PubMed","id":"15919996"},{"database":"DOI","id":"10.1126/science.1109730"}],"title":"Global topology analysis of the Escherichia coli inner membrane proteome.","publicationDate":"2005","journal":"Science","firstPage":"1321","lastPage":"1323","volume":"308"},"referencePositions":["TOPOLOGY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X68301","properties":[{"key":"ProteinId","value":"CAA48369.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC75340.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA16108.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"F64999","properties":[{"key":"EntryName","value":"F64999"}]},{"database":"RefSeq","id":"NP_416783.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000393511.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"PDB","id":"7NYH","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.60 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7NYR","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.30 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7NYU","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.80 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7NYV","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.70 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7P61","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.20 A"},{"key":"Chains","value":"J=1-175"}]},{"database":"PDB","id":"7P62","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.60 A"},{"key":"Chains","value":"J=1-175"}]},{"database":"PDB","id":"7P63","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.40 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7P64","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.50 A"},{"key":"Chains","value":"J=1-162"}]},{"database":"PDB","id":"7P69","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"J=1-162"}]},{"database":"PDB","id":"7P7C","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.40 A"},{"key":"Chains","value":"J=1-162"}]},{"database":"PDB","id":"7P7E","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7P7J","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7P7K","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.10 A"},{"key":"Chains","value":"J=1-162"}]},{"database":"PDB","id":"7P7L","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"J=1-162"}]},{"database":"PDB","id":"7P7M","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.20 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7Z7R","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.36 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7Z7S","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.40 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7Z7T","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.10 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7Z7V","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.29 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7Z80","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.93 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7Z83","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.88 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7Z84","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.87 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7ZC5","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"7ZCI","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.69 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"9Q8I","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.35 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"9TAJ","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"9TAK","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.80 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"9TAM","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.96 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDB","id":"9TAO","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.61 A"},{"key":"Chains","value":"J=1-184"}]},{"database":"PDBsum","id":"7NYH","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7NYR","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7NYU","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7NYV","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P61","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P62","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P63","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P64","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P69","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7C","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7E","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7J","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7K","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7L","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7M","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7R","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7S","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7T","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7V","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z80","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z83","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z84","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7ZC5","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7ZCI","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9Q8I","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAJ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAK","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAM","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAO","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P0AFE0","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-12652","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-12653","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-12654","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-12655","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-52895","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0AFE0","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4260509","properties":[{"key":"Interactions","value":"43"}]},{"database":"ComplexPortal","id":"CPX-243","properties":[{"key":"EntryName","value":"Respiratory chain complex I"}]},{"database":"DIP","id":"DIP-59258N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P0AFE0","properties":[{"key":"Number of interactors","value":"436"}]},{"database":"IntAct","id":"P0AFE0","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"511145.b2280","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"3.D.1.1.1","properties":[{"key":"FamilyName","value":"the h+ or na+-translocating nadh dehydrogenase (ndh) family"}]},{"database":"jPOST","id":"P0AFE0","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b2280","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC75340","properties":[{"key":"ProteinId","value":"AAC75340"},{"key":"GeneId","value":"b2280"}]},{"database":"GeneID","id":"86947217","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"93774894","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"946756","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW2275","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b2280","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_12725","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.4456","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB2014","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0839","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_085957_0_1_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P0AFE0","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AVQFWIL","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9790848at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P0AFE0","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:NUOJ-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:NUOJ-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P0AFE0","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P0AFE0","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16079137"}]},{"database":"GO","id":"GO:0030964","properties":[{"key":"GoTerm","value":"C:NADH dehydrogenase complex"},{"key":"GoEvidenceType","value":"IDA:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"7607227"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15919996"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17309111"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"7607227"}]},{"database":"GO","id":"GO:0045271","properties":[{"key":"GoTerm","value":"C:respiratory chain complex I"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17722886"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"15736965"}]},{"database":"GO","id":"GO:0022904","properties":[{"key":"GoTerm","value":"P:respiratory electron transport chain"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18316732"}]},{"database":"DisProt","id":"DP03503","properties":[{"key":"Description","value":"-"}]},{"database":"FunFam","id":"1.20.120.1200:FF:000001","properties":[{"key":"EntryName","value":"NADH-quinone oxidoreductase subunit J"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.120.1200","properties":[{"key":"EntryName","value":"NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001457","properties":[{"key":"EntryName","value":"NADH_UbQ/plastoQ_OxRdtase_su6"}]},{"database":"InterPro","id":"IPR042106","properties":[{"key":"EntryName","value":"Nuo/plastoQ_OxRdtase_6_NuoJ"}]},{"database":"NCBIfam","id":"NF005162","properties":[{"key":"EntryName","value":"PRK06638.1-1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33269","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33269:SF17","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00499","properties":[{"key":"EntryName","value":"Oxidored_q3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFFSLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAIMLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGREERAGEVLSNRKDDSAKRKTEEHA","length":184,"molWeight":19875,"crc64":"9C9D0C9ABAEC7755","md5":"DADB23ACA088493E208AD39C45DDA147"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":5,"Topological domain":5,"Sequence conflict":2,"Helix":11,"Turn":1},"uniParcId":"UPI0000130A08"}}