{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0AFC7","secondaryAccessions":["P33598","P78090","P78186","P78187"],"uniProtkbId":"NUOB_ECOLI","entryAudit":{"firstPublicDate":"2005-12-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-12-20","entryVersion":141,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01356"}],"value":"NADH-quinone oxidoreductase subunit B"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01356"}],"value":"7.1.1.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01356"}],"value":"NADH dehydrogenase I subunit B"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01356"}],"value":"NDH-1 subunit B"}},{"fullName":{"value":"NUO2"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01356"}],"value":"nuoB"},"orderedLocusNames":[{"value":"b2287"},{"value":"JW5875"}]}],"comments":[{"texts":[{"value":"NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a quinone + NADH + 5 H(+)(in) = a quinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:57888"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:24646"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:132124"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01356"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"[4Fe-4S] cluster","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01356"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:49883"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01356"}],"value":"Binds 1 [4Fe-4S] cluster."}]}},{"texts":[{"value":"NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Cell inner membrane","id":"SL-0037"},"topology":{"value":"Peripheral membrane protein","id":"SL-9903"},"orientation":{"value":"Cytoplasmic side","id":"SL-9910"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01356"}],"value":"Belongs to the complex I 20 kDa subunit family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"NADH-quinone oxidoreductase subunit B","featureId":"PRO_0000118765"},{"type":"Binding site","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01356"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}},{"type":"Binding site","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":64,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01356"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}},{"type":"Binding site","location":{"start":{"value":129,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01356"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}},{"type":"Binding site","location":{"start":{"value":158,"modifier":"EXACT"},"end":{"value":158,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01356"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}},{"type":"Sequence conflict","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":71,"modifier":"EXACT"}},"description":"in Ref. 1; CAA48361","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["L"]}},{"type":"Beta strand","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":6,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Beta strand","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":18,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7S"}]},{"type":"Beta strand","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":25,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7S"}]},{"type":"Helix","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Beta strand","location":{"start":{"value":56,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Beta strand","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":64,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Helix","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Helix","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7AWT"}]},{"type":"Turn","location":{"start":{"value":74,"modifier":"EXACT"},"end":{"value":76,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Beta strand","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Helix","location":{"start":{"value":90,"modifier":"EXACT"},"end":{"value":92,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7P7C"}]},{"type":"Beta strand","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":98,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Turn","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":106,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Helix","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Beta strand","location":{"start":{"value":122,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Helix","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Beta strand","location":{"start":{"value":141,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7P7M"}]},{"type":"Helix","location":{"start":{"value":145,"modifier":"EXACT"},"end":{"value":147,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Beta strand","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Beta strand","location":{"start":{"value":157,"modifier":"EXACT"},"end":{"value":159,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Helix","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":176,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Beta strand","location":{"start":{"value":178,"modifier":"EXACT"},"end":{"value":180,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7P61"}]},{"type":"Beta strand","location":{"start":{"value":183,"modifier":"EXACT"},"end":{"value":188,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7Z7V"}]},{"type":"Turn","location":{"start":{"value":193,"modifier":"EXACT"},"end":{"value":197,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Helix","location":{"start":{"value":200,"modifier":"EXACT"},"end":{"value":203,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]},{"type":"Helix","location":{"start":{"value":205,"modifier":"EXACT"},"end":{"value":209,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7NZ1"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0004","category":"Ligand","name":"4Fe-4S"},{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0411","category":"Ligand","name":"Iron-sulfur"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0520","category":"Ligand","name":"NAD"},{"id":"KW-0874","category":"PTM","name":"Quinone"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"id":"KW-0813","category":"Biological process","name":"Transport"},{"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"7690854","citationType":"journal article","authors":["Weidner U.","Geier S.","Ptock A.","Friedrich T.","Leif H.","Weiss H."],"citationCrossReferences":[{"database":"PubMed","id":"7690854"},{"database":"DOI","id":"10.1006/jmbi.1993.1488"}],"title":"The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I.","publicationDate":"1993","journal":"J. Mol. Biol.","firstPage":"109","lastPage":"122","volume":"233"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"K12 / AN387","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9205837","citationType":"journal article","authors":["Yamamoto Y.","Aiba H.","Baba T.","Hayashi K.","Inada T.","Isono K.","Itoh T.","Kimura S.","Kitagawa M.","Makino K.","Miki T.","Mitsuhashi N.","Mizobuchi K.","Mori H.","Nakade S.","Nakamura Y.","Nashimoto H.","Oshima T.","Oyama S.","Saito N.","Sampei G.","Satoh Y.","Sivasundaram S.","Tagami H.","Takahashi H.","Takeda J.","Takemoto K.","Uehara K.","Wada C.","Yamagata S.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9205837"},{"database":"DOI","id":"10.1093/dnares/4.2.91"}],"title":"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.","publicationDate":"1997","journal":"DNA Res.","firstPage":"91","lastPage":"113","volume":"4"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]","SEQUENCE REVISION"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"9298646","citationType":"journal article","authors":["Link A.J.","Robison K.","Church G.M."],"citationCrossReferences":[{"database":"PubMed","id":"9298646"},{"database":"DOI","id":"10.1002/elps.1150180807"}],"title":"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.","publicationDate":"1997","journal":"Electrophoresis","firstPage":"1259","lastPage":"1313","volume":"18"},"referencePositions":["PROTEIN SEQUENCE OF 1-12"],"referenceComments":[{"value":"K12 / EMG2","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"7607227","citationType":"journal article","authors":["Leif H.","Sled V.D.","Ohnishi T.","Weiss H.","Friedrich T."],"citationCrossReferences":[{"database":"PubMed","id":"7607227"},{"database":"DOI","id":"10.1111/j.1432-1033.1995.tb20594.x"}],"title":"Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli.","publicationDate":"1995","journal":"Eur. J. Biochem.","firstPage":"538","lastPage":"548","volume":"230"},"referencePositions":["PROTEIN SEQUENCE OF 1-6"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X68301","properties":[{"key":"ProteinId","value":"CAA48361.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC75347.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA16121.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"E65000","properties":[{"key":"EntryName","value":"E65000"}]},{"database":"RefSeq","id":"NP_416790.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000386733.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"RefSeq","id":"WP_046608242.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_LN832404.1"}]},{"database":"PDB","id":"7AWT","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.73 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7NYR","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.30 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7NYU","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.80 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7NYV","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.70 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7NZ1","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.10 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7P61","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.20 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7P62","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.60 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7P63","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.40 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7P64","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.50 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7P69","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7P7C","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.40 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7P7E","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7P7J","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7P7K","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.10 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7P7L","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7P7M","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.20 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7Z7R","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.36 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7Z7S","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.40 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7Z7T","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.10 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7Z7V","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.29 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7Z80","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.93 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7Z83","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.88 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7Z84","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.87 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7ZC5","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"7ZCI","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.69 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"9Q8I","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.35 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"9TAJ","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"9TAK","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.80 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"9TAL","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.66 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"9TAM","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.96 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDB","id":"9TAN","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.59 A"},{"key":"Chains","value":"B=1-220"}]},{"database":"PDBsum","id":"7AWT","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7NYR","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7NYU","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7NYV","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7NZ1","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P61","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P62","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P63","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P64","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P69","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7C","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7E","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7J","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7K","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7L","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7P7M","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7R","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7S","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7T","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z7V","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z80","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z83","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7Z84","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7ZC5","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7ZCI","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9Q8I","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAJ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAK","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAL","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAM","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9TAN","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P0AFC7","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-12653","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-12654","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-12655","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-12661","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-52895","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0AFC7","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4262975","properties":[{"key":"Interactions","value":"66"}]},{"database":"ComplexPortal","id":"CPX-243","properties":[{"key":"EntryName","value":"Respiratory chain complex I"}]},{"database":"FunCoup","id":"P0AFC7","properties":[{"key":"Number of interactors","value":"569"}]},{"database":"IntAct","id":"P0AFC7","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"511145.b2287","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"3.D.1.1.1","properties":[{"key":"FamilyName","value":"the h+ or na+-translocating nadh dehydrogenase (ndh) family"}]},{"database":"jPOST","id":"P0AFC7","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b2287","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC75347","properties":[{"key":"ProteinId","value":"AAC75347"},{"key":"GeneId","value":"b2287"}]},{"database":"GeneID","id":"67176143","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"93774887","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"946738","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW5875","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b2287","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_12760","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.4449","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB2008","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0377","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_055737_7_3_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P0AFC7","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"CGGPYWE","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9786737at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P0AFC7","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:NUOB-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:NUOB-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P0AFC7","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P0AFC7","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16079137"}]},{"database":"GO","id":"GO:0030964","properties":[{"key":"GoTerm","value":"C:NADH dehydrogenase complex"},{"key":"GoEvidenceType","value":"IDA:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"7607227"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"7607227"}]},{"database":"GO","id":"GO:0045271","properties":[{"key":"GoTerm","value":"C:respiratory chain complex I"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"11997136"}]},{"database":"GO","id":"GO:0051539","properties":[{"key":"GoTerm","value":"F:4 iron, 4 sulfur cluster binding"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"12975362"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0050136","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (quinone) (non-electrogenic) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0048038","properties":[{"key":"GoTerm","value":"F:quinone binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0009060","properties":[{"key":"GoTerm","value":"P:aerobic respiration"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"9495756"}]},{"database":"GO","id":"GO:0015990","properties":[{"key":"GoTerm","value":"P:electron transport coupled proton transport"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"16807239"}]},{"database":"GO","id":"GO:0022904","properties":[{"key":"GoTerm","value":"P:respiratory electron transport chain"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18316732"}]},{"database":"DisProt","id":"DP03500","properties":[{"key":"Description","value":"-"}]},{"database":"FunFam","id":"3.40.50.12280:FF:000002","properties":[{"key":"EntryName","value":"NADH-quinone oxidoreductase subunit B"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.12280","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01356","properties":[{"key":"EntryName","value":"NDH1_NuoB"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006137","properties":[{"key":"EntryName","value":"NADH_UbQ_OxRdtase-like_20kDa"}]},{"database":"InterPro","id":"IPR006138","properties":[{"key":"EntryName","value":"NADH_UQ_OxRdtase_20Kd_su"}]},{"database":"NCBIfam","id":"TIGR01957","properties":[{"key":"EntryName","value":"nuoB_fam"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF005012","properties":[{"key":"EntryName","value":"PRK06411.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11995","properties":[{"key":"EntryName","value":"NADH DEHYDROGENASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11995:SF14","properties":[{"key":"EntryName","value":"NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01058","properties":[{"key":"EntryName","value":"Oxidored_q6"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56770","properties":[{"key":"EntryName","value":"HydA/Nqo6-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01150","properties":[{"key":"EntryName","value":"COMPLEX1_20K"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLNDMVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPPRPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPDEI","length":220,"molWeight":25056,"crc64":"EBD6268505993880","md5":"F9CF5EF975A24D6CD284EBCE2BD80383"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":4,"Sequence conflict":1,"Beta strand":13,"Helix":11,"Turn":3},"uniParcId":"UPI00001309D6"}}