{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0AF63","secondaryAccessions":["P21498","P72442","Q2M6C7"],"uniProtkbId":"NSRR_ECOLI","entryAudit":{"firstPublicDate":"2005-12-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-12-20","entryVersion":140,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"HTH-type transcriptional repressor NsrR"}}},"genes":[{"geneName":{"value":"nsrR"},"synonyms":[{"value":"yjeB"}],"orderedLocusNames":[{"value":"b4178"},{"value":"JW4136"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16428390"}],"value":"Nitric oxide-sensitive repressor of genes involved in protecting the cell against nitrosative stress, such as ytfE, hmpA and ygbA. May require iron for activity. Does not regulate its own transcription"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"[2Fe-2S] cluster","evidences":[{"evidenceCode":"ECO:0000305"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:190135"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Binds 1 [2Fe-2S] cluster per subunit."}]}}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":141,"modifier":"EXACT"}},"description":"HTH-type transcriptional repressor NsrR","featureId":"PRO_0000109555"},{"type":"Domain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"HTH rrf2-type"},{"type":"DNA binding","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"H-T-H motif","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Binding site","location":{"start":{"value":91,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:190135"}],"evidences":[{"evidenceCode":"ECO:0000255"}],"ligand":{"name":"[2Fe-2S] cluster","id":"ChEBI:CHEBI:190135"}},{"type":"Binding site","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:190135"}],"evidences":[{"evidenceCode":"ECO:0000255"}],"ligand":{"name":"[2Fe-2S] cluster","id":"ChEBI:CHEBI:190135"}},{"type":"Binding site","location":{"start":{"value":102,"modifier":"EXACT"},"end":{"value":102,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:190135"}],"evidences":[{"evidenceCode":"ECO:0000255"}],"ligand":{"name":"[2Fe-2S] cluster","id":"ChEBI:CHEBI:190135"}}],"keywords":[{"id":"KW-0001","category":"Ligand","name":"2Fe-2S"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0411","category":"Ligand","name":"Iron-sulfur"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0678","category":"Molecular function","name":"Repressor"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"}],"references":[{"referenceNumber":1,"citation":{"id":"3058695","citationType":"journal article","authors":["Wolfe S.A.","Smith J.M."],"citationCrossReferences":[{"database":"PubMed","id":"3058695"},{"database":"DOI","id":"10.1016/s0021-9258(18)37402-7"}],"title":"Nucleotide sequence and analysis of the purA gene encoding adenylosuccinate synthetase of Escherichia coli K12.","publicationDate":"1988","journal":"J. Biol. Chem.","firstPage":"19147","lastPage":"19153","volume":"263"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"K12","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"7610040","citationType":"journal article","authors":["Burland V.D.","Plunkett G. III","Sofia H.J.","Daniels D.L.","Blattner F.R."],"citationCrossReferences":[{"database":"PubMed","id":"7610040"},{"database":"DOI","id":"10.1093/nar/23.12.2105"}],"title":"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.","publicationDate":"1995","journal":"Nucleic Acids Res.","firstPage":"2105","lastPage":"2119","volume":"23"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"16428390","citationType":"journal article","authors":["Bodenmiller D.M.","Spiro S."],"citationCrossReferences":[{"database":"PubMed","id":"16428390"},{"database":"DOI","id":"10.1128/jb.188.3.874-881.2006"}],"title":"The yjeB (nsrR) gene of Escherichia coli encodes a nitric oxide-sensitive transcriptional regulator.","publicationDate":"2006","journal":"J. Bacteriol.","firstPage":"874","lastPage":"881","volume":"188"},"referencePositions":["FUNCTION"],"referenceComments":[{"value":"K12 / MC1000 / ATCC 39531","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"J04199","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U14003","properties":[{"key":"ProteinId","value":"AAA97074.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC77135.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE78179.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S56403","properties":[{"key":"EntryName","value":"S56403"}]},{"database":"RefSeq","id":"NP_418599.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001177639.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P0AF63","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0AF63","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4262026","properties":[{"key":"Interactions","value":"151"}]},{"database":"DIP","id":"DIP-48211N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P0AF63","properties":[{"key":"Number of interactors","value":"322"}]},{"database":"IntAct","id":"P0AF63","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"511145.b4178","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P0AF63","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b4178","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"948693","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC77135","properties":[{"key":"ProteinId","value":"AAC77135"},{"key":"GeneId","value":"b4178"}]},{"database":"GeneID","id":"67175203","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"93777643","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"948693","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW4136","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b4178","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_22575","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.2523","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB1197","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG1959","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_107144_2_1_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P0AF63","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AQEAFYA","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9795923at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P0AF63","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:EG11212-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P0AF63","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P0AF63","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0003700","properties":[{"key":"GoTerm","value":"F:DNA-binding transcription factor activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0003690","properties":[{"key":"GoTerm","value":"F:double-stranded DNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0045892","properties":[{"key":"GoTerm","value":"P:negative regulation of DNA-templated transcription"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006355","properties":[{"key":"GoTerm","value":"P:regulation of DNA-templated transcription"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"16428390"}]},{"database":"FunFam","id":"1.10.10.10:FF:000105","properties":[{"key":"EntryName","value":"HTH-type transcriptional repressor NsrR"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.10.10","properties":[{"key":"EntryName","value":"Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01177","properties":[{"key":"EntryName","value":"HTH_type_NsrR"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR030489","properties":[{"key":"EntryName","value":"TR_Rrf2-type_CS"}]},{"database":"InterPro","id":"IPR000944","properties":[{"key":"EntryName","value":"Tscrpt_reg_Rrf2"}]},{"database":"InterPro","id":"IPR023761","properties":[{"key":"EntryName","value":"Tscrpt_rep_HTH_NsrR"}]},{"database":"InterPro","id":"IPR036388","properties":[{"key":"EntryName","value":"WH-like_DNA-bd_sf"}]},{"database":"InterPro","id":"IPR036390","properties":[{"key":"EntryName","value":"WH_DNA-bd_sf"}]},{"database":"NCBIfam","id":"NF008240","properties":[{"key":"EntryName","value":"PRK11014.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00738","properties":[{"key":"EntryName","value":"rrf2_super"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33221:SF4","properties":[{"key":"EntryName","value":"HTH-TYPE TRANSCRIPTIONAL REPRESSOR NSRR"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33221","properties":[{"key":"EntryName","value":"WINGED HELIX-TURN-HELIX TRANSCRIPTIONAL REGULATOR, RRF2 FAMILY"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02082","properties":[{"key":"EntryName","value":"Rrf2"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF46785","properties":[{"key":"EntryName","value":"Winged helix' DNA-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01332","properties":[{"key":"EntryName","value":"HTH_RRF2_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51197","properties":[{"key":"EntryName","value":"HTH_RRF2_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVKIINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPACRLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE","length":141,"molWeight":15593,"crc64":"6A783840023134D7","md5":"26784DC35C190B306C0852D4DEBC0EF8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1},"countByFeatureType":{"Chain":1,"Domain":1,"DNA binding":1,"Binding site":3},"uniParcId":"UPI000013B551"}}