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Poorly inhibited by 5'-isobutylthioinosine, 5'-n-butylthioinosine, 5'-methylthio-3-deaza-adenosine, 5'-isobutylthio-3-deaza-adenosine, S-n-6-methyl-3-deaza-adenosylhomocysteine, S-adenosylhomocysteine sulphoxide and Sinefungin"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":0.4,"unit":"uM","substrate":"5'-methylthioadenosine (at pH 7 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"3911944"}]},{"constant":0.8,"unit":"uM","substrate":"5'-methylthioadenosine (at pH 7 and 22 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16101288"}]},{"constant":4.3,"unit":"uM","substrate":"S-adenosylhomocysteine (at pH 7 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"3911944"}]},{"constant":1.3,"unit":"uM","substrate":"S-adenosylhomocysteine (at pH 7 and 22 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16101288"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16101288"}],"value":"kcat is 3.0 sec(-1) and 2.6 sec(-1) with 5'-methylthioadenosine and S-adenosylhomocysteine as substrate, respectively."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8941345"}],"value":"Exhibits activity across a broad pH range. 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