{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0AEY1","secondaryAccessions":["P31123","P31124","P77753"],"uniProtkbId":"MARC_ECOLI","entryAudit":{"firstPublicDate":"2005-12-20","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2005-12-20","entryVersion":119,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"UPF0056 inner membrane protein MarC"}}},"genes":[{"geneName":{"value":"marC"},"synonyms":[{"value":"ydeB"},{"value":"ydeC"}],"orderedLocusNames":[{"value":"b1529"},{"value":"JW1522"}]}],"comments":[{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Cell inner membrane","id":"SL-0037"},"topology":{"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the UPF0056 (MarC) family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"8383113"}],"value":"Was originally thought to be involved in multiple antibiotic resistance"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":221,"modifier":"EXACT"}},"description":"UPF0056 inner membrane protein MarC","featureId":"PRO_0000156897"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":7,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":90,"modifier":"EXACT"},"end":{"value":118,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":119,"modifier":"EXACT"},"end":{"value":139,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":140,"modifier":"EXACT"},"end":{"value":154,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":176,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":197,"modifier":"EXACT"},"end":{"value":217,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":218,"modifier":"EXACT"},"end":{"value":221,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]}],"keywords":[{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"8383113","citationType":"journal article","authors":["Cohen S.P.","Haechler H.","Levy S.B."],"citationCrossReferences":[{"database":"PubMed","id":"8383113"},{"database":"DOI","id":"10.1128/jb.175.5.1484-1492.1993"}],"title":"Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli.","publicationDate":"1993","journal":"J. Bacteriol.","firstPage":"1484","lastPage":"1492","volume":"175"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":2,"citation":{"id":"9097039","citationType":"journal article","authors":["Aiba H.","Baba T.","Fujita K.","Hayashi K.","Inada T.","Isono K.","Itoh T.","Kasai H.","Kashimoto K.","Kimura S.","Kitakawa M.","Kitagawa M.","Makino K.","Miki T.","Mizobuchi K.","Mori H.","Mori T.","Motomura K.","Nakade S.","Nakamura Y.","Nashimoto H.","Nishio Y.","Oshima T.","Saito N.","Sampei G.","Seki Y.","Sivasundaram S.","Tagami H.","Takeda J.","Takemoto K.","Takeuchi Y.","Wada C.","Yamamoto Y.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9097039"},{"database":"DOI","id":"10.1093/dnares/3.6.363"}],"title":"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.","publicationDate":"1996","journal":"DNA Res.","firstPage":"363","lastPage":"377","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-BSVI8J0OH60M4","citationType":"submission","authors":["Haechler H."],"publicationDate":"MAY-1998","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENE NAME"]},{"referenceNumber":6,"citation":{"id":"11867724","citationType":"journal article","authors":["Drew D.","Sjoestrand D.","Nilsson J.","Urbig T.","Chin C.-N.","de Gier J.-W.","von Heijne G."],"citationCrossReferences":[{"database":"PubMed","id":"11867724"},{"database":"DOI","id":"10.1073/pnas.052018199"}],"title":"Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis.","publicationDate":"2002","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"2690","lastPage":"2695","volume":"99"},"referencePositions":["TOPOLOGY"],"referenceComments":[{"value":"K12 / JM109 / ATCC 53323","type":"STRAIN"}]},{"referenceNumber":7,"citation":{"id":"15919996","citationType":"journal article","authors":["Daley D.O.","Rapp M.","Granseth E.","Melen K.","Drew D.","von Heijne G."],"citationCrossReferences":[{"database":"PubMed","id":"15919996"},{"database":"DOI","id":"10.1126/science.1109730"}],"title":"Global topology analysis of the Escherichia coli inner membrane proteome.","publicationDate":"2005","journal":"Science","firstPage":"1321","lastPage":"1323","volume":"308"},"referencePositions":["TOPOLOGY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":8,"citation":{"id":"17954692","citationType":"journal article","authors":["McDermott P.F.","McMurry L.M.","Podglajen I.","Dzink-Fox J.L.","Schneiders T.","Draper M.P.","Levy S.B."],"citationCrossReferences":[{"database":"PubMed","id":"17954692"},{"database":"DOI","id":"10.1128/aac.00930-07"}],"title":"The marC gene of Escherichia coli is not involved in multiple antibiotic resistance.","publicationDate":"2008","journal":"Antimicrob. Agents Chemother.","firstPage":"382","lastPage":"383","volume":"52"},"referencePositions":["LACK OF INVOLVEMENT IN ANTIBIOTIC RESISTANCE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"M96235","properties":[{"key":"ProteinId","value":"AAC16396.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC74602.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA15211.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"D64907","properties":[{"key":"EntryName","value":"D64907"}]},{"database":"RefSeq","id":"NP_416046.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000885033.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P0AEY1","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4259109","properties":[{"key":"Interactions","value":"12"}]},{"database":"FunCoup","id":"P0AEY1","properties":[{"key":"Number of interactors","value":"132"}]},{"database":"STRING","id":"511145.b1529","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"2.A.95.1.1","properties":[{"key":"FamilyName","value":"the 6 tms neutral amino acid transporter (naat) family"}]},{"database":"PaxDb","id":"511145-b1529","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC74602","properties":[{"key":"ProteinId","value":"AAC74602"},{"key":"GeneId","value":"b1529"}]},{"database":"GeneID","id":"93775693","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"947132","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW1522","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b1529","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_08835","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.737","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB1593","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG2095","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_079909_2_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P0AEY1","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"MLIMIDP","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"21094at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P0AEY1","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:EG11637-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P0AEY1","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"InterPro","id":"IPR002771","properties":[{"key":"EntryName","value":"Multi_antbiot-R_MarC"}]},{"database":"NCBIfam","id":"TIGR00427","properties":[{"key":"EntryName","value":"NAAT family transporter"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF008228","properties":[{"key":"EntryName","value":"PRK10995.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33508:SF2","properties":[{"key":"EntryName","value":"UPF0056 INNER MEMBRANE PROTEIN MARC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33508","properties":[{"key":"EntryName","value":"UPF0056 MEMBRANE PROTEIN YHCE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01914","properties":[{"key":"EntryName","value":"MarC"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQSLMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSPEAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAPPLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIKTYH","length":221,"molWeight":23617,"crc64":"7957FF9403C5F135","md5":"35B1C7934308C66F9597DC4890CA4134"},"extraAttributes":{"countByCommentType":{"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Chain":1,"Topological domain":7,"Transmembrane":6},"uniParcId":"UPI0000000D24"}}