{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0ADQ7","secondaryAccessions":["P77800","Q47413"],"uniProtkbId":"YGAM_ECOLI","entryAudit":{"firstPublicDate":"2005-12-06","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2012-11-28","entryVersion":122,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Uncharacterized protein YgaM"}}},"genes":[{"geneName":{"value":"ygaM"},"orderedLocusNames":[{"value":"b2672"},{"value":"JW2647"}]}],"comments":[{"texts":[{"value":"Upon overexpression binds to ribosomes"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Single-pass membrane protein","id":"SL-9904"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22661687"}],"value":"Maximally expressed between 6 and 12 hours of growth, in early stationary phase"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the ElaB/YgaM/YqjD family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAA16537.1","note":"Extended N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"CAA56183.1","note":"Extended N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"CAA56183.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"Uncharacterized protein YgaM","featureId":"PRO_0000169297"},{"type":"Transmembrane","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":106,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]}],"keywords":[{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"8820648","citationType":"journal article","authors":["Jordan A.","Aragall E.","Gibert I.","Barbe J."],"citationCrossReferences":[{"database":"PubMed","id":"8820648"},{"database":"DOI","id":"10.1046/j.1365-2958.1996.424950.x"}],"title":"Promoter identification and expression analysis of Salmonella typhimurium and Escherichia coli nrdEF operons encoding one of two class I ribonucleotide reductases present in both bacteria.","publicationDate":"1996","journal":"Mol. Microbiol.","firstPage":"777","lastPage":"790","volume":"19"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"K12","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9205837","citationType":"journal article","authors":["Yamamoto Y.","Aiba H.","Baba T.","Hayashi K.","Inada T.","Isono K.","Itoh T.","Kimura S.","Kitagawa M.","Makino K.","Miki T.","Mitsuhashi N.","Mizobuchi K.","Mori H.","Nakade S.","Nakamura Y.","Nashimoto H.","Oshima T.","Oyama S.","Saito N.","Sampei G.","Satoh Y.","Sivasundaram S.","Tagami H.","Takahashi H.","Takeda J.","Takemoto K.","Uehara K.","Wada C.","Yamagata S.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9205837"},{"database":"DOI","id":"10.1093/dnares/4.2.91"}],"title":"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.","publicationDate":"1997","journal":"DNA Res.","firstPage":"91","lastPage":"113","volume":"4"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"22661687","citationType":"journal article","authors":["Yoshida H.","Maki Y.","Furuike S.","Sakai A.","Ueta M.","Wada A."],"citationCrossReferences":[{"database":"PubMed","id":"22661687"},{"database":"DOI","id":"10.1128/jb.00396-12"}],"title":"YqjD is an inner membrane protein associated with stationary-phase ribosomes in Escherichia coli.","publicationDate":"2012","journal":"J. Bacteriol.","firstPage":"4178","lastPage":"4183","volume":"194"},"referencePositions":["RIBOSOME-BINDING","INDUCTION"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X79787","properties":[{"key":"ProteinId","value":"CAA56183.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC75719.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA16537.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"A65047","properties":[{"key":"EntryName","value":"A65047"}]},{"database":"RefSeq","id":"NP_417158.4","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001295174.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P0ADQ7","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0ADQ7","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4261135","properties":[{"key":"Interactions","value":"20"}]},{"database":"DIP","id":"DIP-48124N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P0ADQ7","properties":[{"key":"Number of interactors","value":"154"}]},{"database":"IntAct","id":"P0ADQ7","properties":[{"key":"Interactions","value":"5"}]},{"database":"STRING","id":"511145.b2672","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P0ADQ7","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b2672","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC75719","properties":[{"key":"ProteinId","value":"AAC75719"},{"key":"GeneId","value":"b2672"}]},{"database":"GeneID","id":"93779338","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"947157","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW2647","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b2672","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.4069","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB3070","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG4575","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_132623_4_1_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P0ADQ7","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"6522731at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P0ADQ7","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:B0899-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P0ADQ7","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17309111"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0043022","properties":[{"key":"GoTerm","value":"F:ribosome binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"InterPro","id":"IPR043605","properties":[{"key":"EntryName","value":"DUF883_C"}]},{"database":"InterPro","id":"IPR043604","properties":[{"key":"EntryName","value":"DUF883_N"}]},{"database":"InterPro","id":"IPR010279","properties":[{"key":"EntryName","value":"YqjD/ElaB"}]},{"database":"NCBIfam","id":"NF007523","properties":[{"key":"EntryName","value":"PRK10132.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35893:SF4","properties":[{"key":"EntryName","value":"INNER MEMBRANE PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35893","properties":[{"key":"EntryName","value":"INNER MEMBRANE PROTEIN-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05957","properties":[{"key":"EntryName","value":"DUF883"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF19029","properties":[{"key":"EntryName","value":"DUF883_C"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAEAARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGALLSMRKS","length":109,"molWeight":11847,"crc64":"EA684649DB849E49","md5":"88DAFDD2F1E6B9926EE59FD6763D3056"},"extraAttributes":{"countByCommentType":{"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"SIMILARITY":1,"SEQUENCE CAUTION":3},"countByFeatureType":{"Chain":1,"Transmembrane":1},"uniParcId":"UPI00001B5129"}}