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The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron(3+) atoms. EntB is a bifunctional protein that serves as an isochorismate lyase and an aryl carrier protein (ArCP). Catalyzes the conversion of isochorismate to 2,3-dihydro-2,3-dihydroxybenzoate (2,3-diDHB), the precursor of 2,3-dihydroxybenzoate (DHB). In the enterobactin assembly, EntB functions as an aryl carrier protein phosphopantetheinylated near the C terminus by EntD to yield holo-EntB, which is then acylated by EntE with 2,3-dihydroxybenzoyl-AMP to form DHB-holo-EntB. Then this product will serve in the formation of the amide bond between 2,3-dihydroxybenzoate (DHB) and L-serine"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3 2,3-dihydroxybenzoate + 3 L-serine + 6 ATP = enterobactin + 6 AMP + 6 diphosphate + 4 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:30571"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:33384"},{"database":"ChEBI","id":"CHEBI:36654"},{"database":"ChEBI","id":"CHEBI:77805"},{"database":"ChEBI","id":"CHEBI:456215"}],"ecNumber":"6.3.2.14","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16632253"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2139796"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9214294"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9485415"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"isochorismate + H2O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11112"},{"database":"ChEBI","id":"CHEBI:15361"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:29780"},{"database":"ChEBI","id":"CHEBI:58764"}],"ecNumber":"3.3.2.1","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16632253"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2139796"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9214294"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9485415"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16632253"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2139796"}],"value":"Inhibited by 3-[(carboxyethenyl)oxy]-6-hydroxy-1-benzoic acid and 3-[(carboxyethenyl)oxy]benzoic acid"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":18.5,"unit":"umol/min/mg","enzyme":"enzyme (at pH 7 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2139796"}]}],"michaelisConstants":[{"constant":14.7,"unit":"uM","substrate":"isochorismate (at pH 7 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2139796"}]},{"constant":23.0,"unit":"uM","substrate":"4,5-dihydroisochorismate (at pH 7 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2139796"}]},{"constant":86.0,"unit":"uM","substrate":"3-[(carboxyethenyl)oxy]-1-cyclohexene-1-carboxylic acid (at pH 7 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2139796"}]},{"constant":120.0,"unit":"uM","substrate":"3-[(1-carboxylatoethenyl)oxy]-cyclohepta-1,6-diene-1-carboxylate (at pH 7 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2139796"}]},{"constant":280.0,"unit":"uM","substrate":"cis-3-[(carboxyethenyl)oxy]-4-cyclohexene-1-carboxylic acid (at pH 7 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2139796"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2139796"}],"value":"kcat is 600 min(-1) for isochorismatase activity with isochorismate as substrate (at pH 7 and 37 degrees Celsius). kcat is 540 min(-1) for isochorismatase activity with 3-[(1-carboxylatoethenyl)oxy]-cyclohepta-1,6-diene-1-carboxylate as substrate (at pH 7 and 37 degrees Celsius). kcat is 310 min(-1) for isochorismatase activity with 4,5-dihydroisochorismate as substrate (at pH 7 and 37 degrees Celsius)."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2139796"}],"value":"Optimum pH is between 6.5 and 7.5. At pH 5.5, EntB retains 50% of isochorismatase activity."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9214294"}],"value":"Siderophore biosynthesis; enterobactin biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16632253"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2139796"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22365602"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35952947"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9485415"}],"value":"Proteins EntB, EntD, EntE, and EntF form a multienzyme complex called enterobactin synthase. Homodimer (PubMed:16632253). Also forms a specific pairwise interaction with EntC; this interaction likely facilitates substrate channeling to connect the EntB and EntC active sites (PubMed:35952947)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P0ADI4","intActId":"EBI-547993"},"interactantTwo":{"uniProtKBAccession":"P0ADI4","geneName":"entB","intActId":"EBI-547993"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P0ADI4","intActId":"EBI-547993"},"interactantTwo":{"uniProtKBAccession":"P10378","geneName":"entE","intActId":"EBI-550322"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P0ADI4","intActId":"EBI-547993"},"interactantTwo":{"uniProtKBAccession":"P0A8Y8","geneName":"entH","intActId":"EBI-1118982"},"numberOfExperiments":3,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10692387"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2521621"}],"value":"Under conditions of iron deficiency and by the fur protein"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22365602"}],"value":"4'-phosphopantetheine is transferred from CoA to a specific serine of apo-EntB by EntD. 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