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It post-transcriptionally regulates the synthesis of the phosphoglyceride-linked enterobacterial common antigen (ECAPG), greatly inhibiting its synthesis during normal growth (PubMed:34809459). Full function of ElyC requires the presence of cyclic enterobacterial common antigen (ECACYC), the periplasmic form of enterobacterial common antigen (ECA) (PubMed:34809459). ElyC may provide feedback regulation of ECAPG production based on the periplasmic levels of ECACYC (PubMed:34809459). Also plays a critical role in the metabolism of the essential lipid carrier (Und-P) used for cell wall synthesis (PubMed:24391520). It contributes to maintaining reactive oxygen species (ROS) homeostasis in the envelope at lower temperatures (PubMed:35170991)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"24391520"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"34809459"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24391520"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34809459"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35170991"}],"value":"Deletion of the gene increases ECAPG levels (PubMed:34809459). Deletion causes a severe growth defect at low temperature and an elevated frequency of cell lysis resulting from impaired metabolism of the essential lipid precursor required for peptidoglycan synthesis (PubMed:24391520). Cells lacking this gene overproduce hydroxyl radicals (HO) in their envelope compartment and that HO overproduction is directly or indirectly responsible for the peptidoglycan synthesis arrest, cell envelope integrity defects and cell lysis of the mutant (PubMed:35170991)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the ElyC family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":259,"modifier":"EXACT"}},"description":"Envelope biogenesis factor ElyC","featureId":"PRO_0000168768"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"24391520"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"34809459"}]},{"type":"Transmembrane","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":32,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":33,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"24391520"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"34809459"}]},{"type":"Transmembrane","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":259,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"24391520"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"34809459"}]},{"type":"Sequence conflict","location":{"start":{"value":89,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"in Ref. 4; D26440","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["N"]}},{"type":"Sequence conflict","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":170,"modifier":"EXACT"}},"description":"in Ref. 4; D26440","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"GVPREQIITLDLPKDTEEEAA","alternativeSequences":["ACRASKLSPWICQKIPKKKLQ"]}}],"keywords":[{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0961","category":"Biological process","name":"Cell wall biogenesis/degradation"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"8905232","citationType":"journal article","authors":["Oshima T.","Aiba H.","Baba T.","Fujita K.","Hayashi K.","Honjo A.","Ikemoto K.","Inada T.","Itoh T.","Kajihara M.","Kanai K.","Kashimoto K.","Kimura S.","Kitagawa M.","Makino K.","Masuda S.","Miki T.","Mizobuchi K.","Mori H.","Motomura K.","Nakamura Y.","Nashimoto H.","Nishio Y.","Saito N.","Sampei G.","Seki Y.","Tagami H.","Takemoto K.","Wada C.","Yamamoto Y.","Yano M.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"8905232"},{"database":"DOI","id":"10.1093/dnares/3.3.137"}],"title":"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.","publicationDate":"1996","journal":"DNA Res.","firstPage":"137","lastPage":"155","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. 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